Screening for control genes in mouse hippocampus after transient forebrain ischemia using high-density oligonucleotide array.


:In conventional relative gene expression analysis (Northern blotting, RT-PCR, and in situ hybridization), housekeeping genes such as the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and beta-actin genes, whose expression levels are considered stable, have been used as control genes for normalization of RNA quantitation. However, it has been reported that the expression levels of these two control genes are affected by ischemia. Therefore, we have been searching for novel control genes whose expression levels are stable in a mouse model of transient forebrain ischemia. Using the GeneChip Mu6500 array set, we monitored the expression levels of approximately 6000 murine genes in the mouse hippocampus during 24 h of ischemia-reperfusion. To select stable genes, we applied the restricted criterion of a 1.5-fold change in expression level as the threshold. By adding statistical analysis with this criterion, we identified 10 genes as candidates for control genes from the GeneChip data. In this criterion, GAPDH and beta-actin genes were not included in the 10 genes as candidates for control genes. The present findings might be relevant to the use of control genes in quantitation of RNA, particularly in the study of mouse transient forebrain ischemia.


J Pharmacol Sci


Nishida Y,Sugahara-Kobayashi M,Takahashi Y,Nagata T,Ishikawa K,Asai S




Has Abstract


2006-05-01 00:00:00














  • History of the G protein-coupled receptor (GPCR) assays from traditional to a state-of-the-art biosensor assay.

    abstract::The G protein-coupled receptors (GPCRs) form the largest and the most versatile superfamily that share a seven-transmembrane-spanning architecture. GPCR-signaling is involved in vision, taste, olfaction, sympathetic/parasympathetic nervous functions, metabolism, and immune regulation, indicating that GPCRs are extreme...

    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章,评审


    authors: Miyano K,Sudo Y,Yokoyama A,Hisaoka-Nakashima K,Morioka N,Takebayashi M,Nakata Y,Higami Y,Uezono Y

    更新日期:2014-01-01 00:00:00

  • D-Serine ameliorates neonatal PolyI:C treatment-induced emotional and cognitive impairments in adult mice.

    abstract::Polyriboinosinic-polyribocytidilic acid (polyI:C) is a synthetic analog that elicits viral-like immune responses in mammals. We have recently found that polyI:C treatment in neonatal mice induced abnormalities of emotional, cognitive, and sensorimotor gating and dysfunction of glutamatergic neurotransmission in adulth...

    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章


    authors: Nagai T,Yu J,Kitahara Y,Nabeshima T,Yamada K

    更新日期:2012-01-01 00:00:00

  • Interaction between anti-Alzheimer and antipsychotic drugs in modulating extrapyramidal motor disorders in mice.

    abstract::Antipsychotics are often used in conjunction with anti-Alzheimer drugs to treat the behavioral and psychological symptoms of dementia (BPSD). Here, we examined the effects of cholinesterase inhibitors (ChEIs), donepezil and galantamine, on antipsychotic-induced extrapyramidal side effects (EPS) in mice. The effects of...

    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章


    authors: Shimizu S,Mizuguchi Y,Sobue A,Fujiwara M,Morimoto T,Ohno Y

    更新日期:2015-04-01 00:00:00

  • Activation of phosphoinositide 3-kinase, protein kinase C, and extracellular signal-regulated kinase is required for oridonin-enhanced phagocytosis of apoptotic bodies in human macrophage-like U937 cells.

    abstract::Our previous study showed that oridonin isolated from Rabdosia rubescens enhanced phagocytosis of apoptotic cells by macrophage-like U937 cells through tumor necrosis factor (TNF) alpha and interleukin (IL)-1beta release. In this study, we further investigated signaling events involved in oridonin-augmented phagocytos...

    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章


    authors: Liu YQ,You S,Tashiro S,Onodera S,Ikejima T

    更新日期:2005-08-01 00:00:00

  • Unique immunomodulatory effect of paeoniflorin on type I and II macrophages activities.

    abstract::It has been widely accepted that macrophages are divided into M1 "pro-inflammatory" macrophages and M2 "anti-inflammatory" macrophages and an uncontrolled macrophage polarization plays an important role in the pathogenesis of different diseases. As the main substance of total glucosides of peony, paeoniflorin (PF), ha...

    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章


    authors: Zhai T,Sun Y,Li H,Zhang J,Huo R,Li H,Shen B,Li N

    更新日期:2016-03-01 00:00:00

  • New aspects of neurotransmitter release and exocytosis: regulation of neurotransmitter release by phosphorylation.

    abstract::Synaptic transmission is conducted by neurotransmitters released from nerve terminals. Neurotransmitter release is regulated both positively and negatively by multiple mechanisms, and its regulation is believed to be one of the important mechanisms of synaptic plasticity underlying learning and memory. Various protein...

    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章,评审


    authors: Takahashi M,Itakura M,Kataoka M

    更新日期:2003-09-01 00:00:00

  • Neurotoxin A2NTX Blocks Fast Inhibitory and Excitatory Transmitter Release From Presynaptic Terminals.

    abstract::Our recent study showed a possibility that newly developed A2 type botulinum toxin (A2NTX) inhibits both spontaneous and evoked transmitter release from inhibitory (glycinergic or GABAergic) and excitatory (glutamatergic) nerve terminals using rat spinal sacral dorsal commissural nucleus neurons. In the present study,...

    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章


    authors: Yamaga T,Aou S,Shin MC,Wakita M,Akaike N

    更新日期:2012-01-01 00:00:00

  • Roxatidine- and cimetidine-induced angiogenesis inhibition suppresses growth of colon cancer implants in syngeneic mice.

    abstract::Cimetidine is known to suppress the growth of several tumors, including gastrointestinal cancer, in humans and animals. Nonetheless, whether other histamine H(2)-receptor antagonists exert such tumor suppressive effects remains unclear. The effect of roxatidine acetate hydrochloride (roxatidine), an H(2)-receptor anta...

    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章


    authors: Tomita K,Izumi K,Okabe S

    更新日期:2003-11-01 00:00:00

  • The effect of antioxidant on development of fibrosis by cisplatin in rats.

    abstract::Cisplatin causes chronic interstitial disease with fibrosis, but the development mechanism of interstitial fibrosis is not yet understood. We examined the effect of an antioxidant, N,N'-diphenyl-1,4-phenylenediamine (DPPD), on development of interstitial fibrosis induced by cisplatin. Cisplatin increased blood urea ni...

    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章


    authors: Kawai Y,Satoh T,Hibi D,Ohno Y,Kohda Y,Miura K,Gemba M

    更新日期:2009-12-01 00:00:00

  • Colonic hydrogen sulfide-induced visceral pain and referred hyperalgesia involve activation of both Ca(v)3.2 and TRPA1 channels in mice.

    abstract::Luminal hydrogen sulfide (H(2)S), a gasotransmitter, causes colonic pain / referred hyperalgesia in mice, most probably via activation of T-type Ca(2+) channels. Here we analyzed the mechanisms for H(2)S-induced facilitation of colonic pain signals. Intracolonic administration of NaHS, an H(2)S donor, evoked visceral ...

    journal_title:Journal of pharmacological sciences

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    authors: Tsubota-Matsunami M,Noguchi Y,Okawa Y,Sekiguchi F,Kawabata A

    更新日期:2012-01-01 00:00:00

  • Cyclic ADP-ribose mediates formyl methionyl leucyl phenylalanine (fMLP)-induced intracellular Ca(2+) rise and migration of human neutrophils.

    abstract::Although cyclic ADP-ribose (cADPR), a novel Ca(2+)-mobilizing mediator, is suggested to be involved in the functions of neutrophils in rodents, its role in human neutrophils remains unclear. The present study examined the ability of cADPR to mobilize Ca(2+) and mediate formyl methionyl leucyl phenylalanine (fMLP)-stim...

    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章


    authors: Morita K,Saida M,Morioka N,Kitayama T,Akagawa Y,Dohi T

    更新日期:2008-03-01 00:00:00

  • Different chronotherapeutic effects of valsartan and olmesartan in non-dipper hypertensive patients during valsartan treatment at morning.

    abstract::This study was undertaken to evaluate the differences in chronotherapeutic effects of angiotensin-II receptor blockers, valsartan and olmesartan in hypertensive patients with non-dipper blood pressure (BP) pattern during valsartan at morning. Ninety four patients were enrolled, and 40 patients were judged to be non-di...

    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Ushijima K,Nakashima H,Shiga T,Harada K,Ishikawa S,Ioka T,Ando H,Fujimura A

    更新日期:2015-01-01 00:00:00

  • QT PRODACT: usability of miniature pigs in safety pharmacology studies: assessment for drug-induced QT interval prolongation.

    abstract::To investigate whether miniature pigs are useful for evaluating the potential of drugs for drug-induced prolongation of the QT interval, we performed an in vivo QT assay using conscious and unrestricted miniature pigs. Compared with the vehicle average baseline values, haloperidol at 3 and 10 mg/kg, p.o. prolonged the...

    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章


    authors: Kano M,Toyoshi T,Iwasaki S,Kato M,Shimizu M,Ota T

    更新日期:2005-01-01 00:00:00

  • Antidepressant-like effect of venlafaxine is abolished in μ-opioid receptor-knockout mice.

    abstract::Although the opioid system is known to modulate depression-like behaviors, its role in the effects of antidepressants is not yet clear. We investigated the role of μ-opioid receptors (MOPs) in the effects of venlafaxine, a serotonin and norepinephrine reuptake inhibitor, in the forced swim test using MOP-knockout (KO)...

    journal_title:Journal of pharmacological sciences

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    authors: Ide S,Fujiwara S,Fujiwara M,Sora I,Ikeda K,Minami M,Uhl GR,Ishihara K

    更新日期:2010-01-01 00:00:00

  • Alleviating effects of AS1892802, a Rho kinase inhibitor, on osteoarthritic disorders in rodents.

    abstract::The effects of AS1892802, a selective Rho-associated coiled coil kinase (ROCK) inhibitor, on knee cartilage damage and pain behavior were examined in a rat model of osteoarthritis (OA). Monoiodoacetate (MIA) was intraarticularly injected into the right knee joints of rats. ROCK I and II mRNA levels increased in knee j...

    journal_title:Journal of pharmacological sciences

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    authors: Takeshita N,Yoshimi E,Hatori C,Kumakura F,Seki N,Shimizu Y

    更新日期:2011-01-01 00:00:00

  • Chronopharmacology of angiotensin II-receptor blockers in stroke-prone spontaneously hypertensive rats.

    abstract::Protective effect of valsartan (Val), an angiotensin II (AII)-receptor blocker (ARB), against organ damage is reported to depend on the dosing time in hypertensive patients. Dosing-time-dependent effect of Val on survival of stroke-prone spontaneously hypertensive rats (SHRSP) under a 12-h lighting cycle was examined....

    journal_title:Journal of pharmacological sciences

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    authors: Liu Y,Ushijima K,Ohmori M,Takada M,Tateishi M,Ando H,Fujimura A

    更新日期:2011-01-01 00:00:00

  • Protective effect of T3 peptide, an active fragment of tumstatin, against ischemia/reperfusion injury in rat heart.

    abstract::Ischemia/reperfusion (I/R)-induced oxidative stress is a serious clinical problem in the reperfusion therapy for ischemic diseases. Tumstatin is an endogenous bioactive peptide cleaved from type IV collagen α3 chain. We previously reported that T3 peptide, an active subfragment of tumstatin, exerts cytoprotective effe...

    journal_title:Journal of pharmacological sciences

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    authors: Yasuda J,Okada M,Yamawaki H

    更新日期:2019-03-01 00:00:00

  • The novel compounds that activate farnesoid X receptor: the diversity of their effects on gene expression.

    abstract::Farnesoid X receptor (FXR) controls the expression of critical genes in bile acid and cholesterol homeostasis. To study FXR and to develop a regulator of cholesterol, some non-steroidal and steroidal ligands have been found in addition to endogenous ligands for FXR. In this study, we discovered five bile acid derivati...

    journal_title:Journal of pharmacological sciences

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    authors: Suzuki T,Tamehiro N,Sato Y,Kobayashi T,Ishii-Watabe A,Shinozaki Y,Nishimaki-Mogami T,Hashimoto T,Asakawa Y,Inoue K,Ohno Y,Yamaguchi T,Kawanishi T

    更新日期:2008-07-01 00:00:00

  • Antihistamines suppress upregulation of histidine decarboxylase gene expression with potencies different from their binding affinities for histamine H1 receptor in toluene 2,4-diisocyanate-sensitized rats.

    abstract::Antihistamines inhibit histamine signaling by blocking histamine H1 receptor (H1R) or suppressing H1R signaling as inverse agonists. The H1R gene is upregulated in patients with pollinosis, and its expression level is correlated with the severity of nasal symptoms. Here, we show that antihistamine suppressed upregulat...

    journal_title:Journal of pharmacological sciences

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    authors: Mizuguchi H,Das AK,Maeyama K,Dev S,Shahriar M,Kitamura Y,Takeda N,Fukui H

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  • Embelin induces apoptosis in human glioma cells through inactivating NF-κB.

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    journal_title:Journal of pharmacological sciences

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    authors: Park SY,Lim SL,Jang HJ,Lee JH,Um JY,Kim SH,Ahn KS,Lee SG

    更新日期:2013-01-01 00:00:00

  • Novel findings for the development of drug therapy for various liver diseases: Current state and future prospects for our liver regeneration therapy using autologous bone marrow cells for decompensated liver cirrhosis patients.

    abstract::We have developed an in vivo mouse model [the green fluorescent protein (GFP) / carbon tetrachloride (CCl(4)) model] and reported that infused GFP-positive bone marrow cells administered via a tail vein efficiently repopulated cirrhotic liver tissue under conditions of persistent liver damage induced by CCl(4). Moreov...

    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章,评审


    authors: Takami T,Terai S,Sakaida I

    更新日期:2011-01-01 00:00:00

  • Bcl-2 family proteins were involved in pseudolaric acid B-induced autophagy in murine fibrosarcoma L929 cells.

    abstract::Pseudolaric acid B (PAB) exerted cytostatic activity on murine fibrosarcoma L929 cells. The cytostatic mechanism of PAB on L929 cells was investigated in this paper. At 36 h, after 80 microM PAB treatment, the inhibitory ratio was 65.37 +/- 4.12%, and the MDC staining ratio was strongest in L929 cells. 3-Methyladenine...

    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章


    authors: Yu J,Li X,Tashiro S,Onodera S,Ikejima T

    更新日期:2008-07-01 00:00:00

  • Genome-wide association study identifies polymorphisms associated with the analgesic effect of fentanyl in the preoperative cold pressor-induced pain test.

    abstract::Opioid analgesics are widely used for the treatment of moderate to severe pain. The analgesic effects of opioids are well known to vary among individuals. The present study focused on the genetic factors that are associated with interindividual differences in pain and opioid sensitivity. We conducted a multistage geno...

    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章


    authors: Takahashi K,Nishizawa D,Kasai S,Koukita Y,Fukuda KI,Ichinohe T,Ikeda K

    更新日期:2018-03-01 00:00:00

  • Anti-tumor activity of Escherichia coli Shiga toxin A subunit delivered by SF9 insect cells.

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    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章


    authors: Oloomi M,Imani M,Behzadi R,Asori M,Bouzari S,Mokhlesi B

    更新日期:2018-09-01 00:00:00

  • Nitric oxide plays a critical role in methotrexate-induced hyperplasia of enterochromaffin cells containing 5-hydroxytryptamine in rat small intestine.

    abstract::The role of nitric oxide (NO) in the changes in enterochromaffin cells and ileal 5-hydroxytryptamine (5-HT) content induced by a single i.p. administration of methotrexate was investigated in rats. Methotrexate significantly increased inducible NO synthase (iNOS) mRNA and protein expressions in the intestinal tissue a...

    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章


    authors: Takano Y,Hirano M,Machida T,Obara Y,Hamaue N,Fujita K,Taniguchi M,Endo T,Shiga S,Machida M,Iizuka K,Hirafuji M

    更新日期:2019-09-01 00:00:00

  • p38 mitogen-activated protein kinase contributes to angiotensin II-stimulated migration of rat aortic smooth muscle cells.

    abstract::In this study, we clarified the intracellular mechanism of angiotensin II (Ang II) in promoting migration in rat aortic smooth muscle cells (RASMCs). RASMC migration was measured with the Boyden chamber assay, and the result was confirmed with an aortic sprout assay. The activities of kinases were investigated by west...

    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章


    authors: Lee HM,Lee CK,Lee SH,Roh HY,Bae YM,Lee KY,Lim J,Park PJ,Park TK,Lee YL,Won KJ,Kim B

    更新日期:2007-09-01 00:00:00

  • Heterotrimeric G protein G alpha13-induced induction of cytokine mRNAs through two distinct pathways in cardiac fibroblasts.

    abstract::Overexpression of constitutively active (CA)-G alpha13 significantly increased the expression of interleukin (IL)-1beta and IL-6 mRNAs and proteins in rat cardiac fibroblasts. IL-1beta mRNA induction by CA-G alpha13 was suppressed by diphenyleneiodonium (DPI), an NADPH oxidase inhibitor, but not by BAPTA-AM, an intrac...

    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章


    authors: Nagamatsu Y,Nishida M,Onohara N,Fukutomi M,Maruyama Y,Kobayashi H,Sato Y,Kurose H

    更新日期:2006-06-01 00:00:00

  • Role of neurosteroid allopregnanolone on age-related differences in exercise-induced hypoalgesia in rats.

    abstract::The beneficial effects of physical activity for pain are denominated exercise-induced hypoalgesia (EIH). Here, we examined the age-related change and potential role of the neurosteroid allopregnanolone (ALLO) on EIH in rats. Adult and aged rats were randomly divided into one of three groups; non-exercise control, Low-...

    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章


    authors: Aoyama B,Kawano T,Iwata H,Nishigaki A,Yamanaka D,Tateiwa H,Shigematsu-Locatelli M,Eguchi S,Locatelli FM,Yokoyama M

    更新日期:2019-02-01 00:00:00

  • Receptor subtypes mediating the inotropic effects and Ca(2+) signaling induced by endothelin-1 through crosstalk with norepinephrine in canine ventricular myocardium.

    abstract::In canine ventricular myocardium, endothelin-1 (ET-1) alone induced only a weak transient negative inotropic effect (NIE). However, ET-1 induced a marked sustained positive inotropic effect (PIE) subsequent to a transient NIE in the presence of norepinephrine (NE) at low concentrations (0.1 - 1 nM) and elicited a pron...

    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章


    authors: Chu L,Zhang JX,Norota I,Endoh M

    更新日期:2005-03-01 00:00:00

  • Age-related effects of dexmedetomidine on myocardial contraction and coronary circulation in isolated guinea pig hearts.

    abstract::Dexmedetomidine is a selective α2 adrenergic agonist. Although dexmedetomidine is widely used for sedation and analgesia, it frequently produces hypotension and bradycardia. The present study aimed to evaluate the effects of dexmedetomidine on cardiac function and coronary circulation using Langendorff-perfused guinea...

    journal_title:Journal of pharmacological sciences

    pub_type: 杂志文章


    authors: Hongo M,Fujisawa S,Adachi T,Shimbo T,Shibata S,Ohba T,Ono K

    更新日期:2016-06-01 00:00:00