Depression of MAD2 inhibits apoptosis of gastric cancer cells by upregulating Bcl-2 and interfering mitochondrion pathway.


:Mitotic arrest deficient 2 (MAD2) is an essential component of the mitotic spindle checkpoint pathway. It was previously shown to be associated with drug resistance of tumor cells. To further explore the roles of MAD2 in responses of gastric cancer cells to chemotherapy drugs, we constructed the siRNA vectors of MAD2 and transfected them into gastric cancer SGC7901 cells to inhibit expression of MAD2. MTT assay showed that the downregulation of MAD2 increased the resistance of SGC7901 cells to spindle inhibitors and DNA damaging agents. The apoptosis rates of gastric cancer cells transfected with MAD2-siRNA were 10.7% and 10%, respectively, after treated by 1.0microg/ml VCR and cisplatin. In contrast, the apoptosis rates of SGC7901 and SGC7901/psilencer3.1 induced by VCR were 43.2%, 38.7%; and that induced by cispaltin were 34.1%, 31.4%. The ratio of Bcl-2 to Bax was much higher in the MAD2-siRNA transfectants compared with the SGC7901/psilencer. In SGC7901/psilencer, cytochrome c and cleaved caspase 3 protein levels increased along with the exposure time increased. However, these protein levels of SGC7901/MAD2-siRNA had no changes during the drug treatment. These results indicate that down regulation of MAD2 could promote the drug resistance of gastric cancer cells and inhibit anticancer drugs induced-apoptosis by upregulating Bcl-2 and interfering the mitochondrion apoptosis pathway.


Du Y,Yin F,Liu C,Hu S,Wang J,Xie H,Hong L,Fan D




Has Abstract


2006-07-07 00:00:00














  • Synemin contains the rod domain of intermediate filaments.

    abstract::We have screened a lambda gt11 cDNA library from chicken gizzard muscle with polyclonal antiserum to avian synemin. Immunopositive clones were characterized and sequenced. Computer searches identified primarily intermediate filament (IF) proteins as being homologous to the synemin clones. Synemin's sequence contained ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Becker B,Bellin RM,Sernett SW,Huiatt TW,Robson RM

    更新日期:1995-08-24 00:00:00

  • Molecular cloning and expression of mouse procalcitonin.

    abstract::The nucleotide sequence for mouse calcitonin was determined from a cDNA obtained using a polymerase chain reaction (PCR) based method, the rapid amplification of cDNA ends (RACE)-PCR. Primers designed from highly conserved regions in the coding sequences of known rat and human calcitonin cDNAs were used to amplify cal...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Rehli M,Luger K,Beier W,Falk W

    更新日期:1996-09-13 00:00:00

  • Modulation of androgen and progesterone receptors by phytochemicals in breast cancer cell lines.

    abstract::We have used a tissue culture system based on breast carcinoma cell lines to investigate a large number of naturally occurring compounds and beverages for steroid hormone agonist and antagonist activity. The cell lines used, T-47D and BT-474, produce prostate specific antigen (PSA) upon stimulation with androgens, pro...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Rosenberg RS,Grass L,Jenkins DJ,Kendall CW,Diamandis EP

    更新日期:1998-07-30 00:00:00

  • Intermediate filament steady-state mRNA levels in amyotrophic lateral sclerosis.

    abstract::We have examined the steady-state levels of intermediate filament mRNA in amyotrophic lateral sclerosis using the RNAse protection assay (NFL, NFM, NFH; corrected against GAPDH) or by PCR (peripherin, alpha-internexin, nestin, and vimentin; corrected against beta-actin). Significant elevations of NFL and peripherin mR...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Strong MJ,Leystra-Lantz C,Ge WW

    更新日期:2004-04-02 00:00:00

  • Artefacts in cell culture: α-Ketoglutarate can scavenge hydrogen peroxide generated by ascorbate and epigallocatechin gallate in cell culture media.

    abstract::Ascorbate and several phenolic compounds readily oxidise in cell culture media to generate hydrogen peroxide. However, addition of α-ketoglutarate, which is known to be released by several cell types, decreased the levels of H(2)O(2), and the α-ketoglutarate was depleted and converted to succinate. These observations ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Long LH,Halliwell B

    更新日期:2011-03-04 00:00:00

  • Evidence of L-arginine/nitric oxide pathway in endothelium and smooth muscle of human internal mammary artery.

    abstract:UNLABELLED:The L-arginine-nitric oxide (NO) pathway was investigated in human internal mammary artery (HIMA) in vitro. HIMA rings were mounted in organ bath, and then incubated in Krebs buffer for 1 to 8 hrs, relaxing agents were tested. Under these conditions, L-arginine (0.1 microM - 1 mM) elicited only minor relaxat...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Qi X,Chen D,Nottin R,Mace L,Herve P,Weiss M

    更新日期:1993-08-31 00:00:00

  • Expression of the calcium-sensing receptor in pancreatic islet B-cells.

    abstract::Exposure of pancreatic islet B-cells to D-glucose and many other insulinotropic agents results in an increase of cytoplasmic calcium concentration, which triggers the exocytosis of secretroy granules. Previous studies have demonstrated that calcium itself, at concentrations ranging from 2 to 18 mM, is able to induce a...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Rasschaert J,Malaisse WJ

    更新日期:1999-11-02 00:00:00

  • Identification of a novel human sterol-sensitive ATP-binding cassette transporter (ABCA7).

    abstract::We report the identification of the full-length cDNA for a novel ATP-binding cassette (ABC) transporter from human macrophages. The mRNA is of 6.8 kb size and contains an open reading frame encoding a polypeptide of 2146 amino acids with a calculated molecular weight of 220 kDa. The predicted protein product is compos...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kaminski WE,Orsó E,Diederich W,Klucken J,Drobnik W,Schmitz G

    更新日期:2000-07-05 00:00:00

  • Poly(rC)-binding protein 2 interacts with the oligo(rC) tract of coxsackievirus B3.

    abstract::The human poly(rC)-binding protein (PCBP) 2 is known to interact with enteroviral RNA. Here, the interaction of PCBP2 with RNA target sequences at the 5' end of the coxsackievirus B3 genome was investigated. Using the electrophoretic mobility shift assay and the yeast three-hybrid system, a short oligo(rC) tract conne...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zell R,Ihle Y,Seitz S,Gündel U,Wutzler P,Görlach M

    更新日期:2008-02-22 00:00:00

  • The role of adenine nucleotide translocation in the energization of the inner membrane of mitochondria isolated from rho + and rho degree strains of Saccharomyces cerevisiae.

    abstract::The energization of rho + and rho degrees isolated mitochondria was measured using a tetraphenylphosphonium selective electrode. In both strains translocase mediated ATP/ADP exchange energization was observed. This energization was more sensitive to uncoupler than that induced by respiration in rho + mitochondria. Thi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Dupont CH,Mazat JP,Guerin B

    更新日期:1985-11-15 00:00:00

  • Characterization of a putative promoter region of the human poly(ADP-ribose) polymerase gene: structural similarity to that of the DNA polymerase beta gene.

    abstract::The 5'-flanking region of the human poly(ADP-ribose) polymerase gene was isolated and characterized. The nucleotide sequence of a part of the poly(ADP-ribose) polymerase gene completely matched that of the cDNA. The transcriptional initiation sites (cap sites) of this gene, located about 166-bp upstream from the trans...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ogura T,Nyunoya H,Takahashi-Masutani M,Miwa M,Sugimura T,Esumi H

    更新日期:1990-03-16 00:00:00

  • Cell cycle regulation of the BRCA1/acetyl-CoA-carboxylase complex.

    abstract::Germ-line alterations in BRCA1 are associated with an increased susceptibility to breast and ovarian cancer. The BRCA1 protein has been implicated in multiple cellular functions. We have recently demonstrated that BRCA1 reduces acetyl-CoA-carboxylase alpha (ACCA) activity through its phospho-dependent binding to ACCA,...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ray H,Suau F,Vincent A,Dalla Venezia N

    更新日期:2009-01-16 00:00:00

  • Transcript decay mediated by RNase III in Borrelia burgdorferi.

    abstract::The causative agent of Lyme disease, Borrelia burgdorferi, requires shifts in gene expression to undergo its natural enzootic cycle between tick and vertebrate hosts. mRNA decay mechanisms play significant roles in governing gene expression in other bacteria, but are not yet characterized in B. burgdorferi. RNase III ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Snow S,Bacon E,Bergeron J,Katzman D,Wilhelm A,Lewis O,Syangtan D,Calkins A,Archambault L,Anacker ML,Schlax PJ

    更新日期:2020-08-20 00:00:00

  • Crystal structure of the PSPTO-PSP protein from Pseudomonas syringae pv. tomato str. DC3000 in complex with D-glucose.

    abstract::The perchloric acid-soluble protein (PSP) is an endoribonuclease and on the basis of sequence similarity has been assigned to the YjgF/YER057c/UK114 family. These family members are ubiquitous and highly conserved in evolution, and participate in regulating basic cellular metabolism. Here we present the 2.1A crystal s...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zhang HM,Gao Y,Li M,Chang WR

    更新日期:2010-06-18 00:00:00

  • Binding and effect of atrial natriuretic factor on cyclic GMP formation and alpha-MSH secretion of intermediate pituitary cells.

    abstract::The present study shows for the first time that in proopiomelanocortin cells of the rat intermediate pituitary gland ANF binds to two receptor forms, with apparent molecular weights of 150K and 70K. Scatchard plots revealed specific and high affinity non-interacting sites, with a KD value of about 3 nM and a density o...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lutz-Bucher B,Boudjada T,Heisler S,Pelletier G,Koch B

    更新日期:1988-08-30 00:00:00

  • Quinolone antibiotics inhibit eucaryotic DNA polymerase alpha and beta, terminal deoxynucleotidyl transferase but not DNA ligase.

    abstract::DNA polymerases alpha and beta, Terminal deoxynucleotidyl Transferase and DNA ligases from chicken thymus were purified to homogeneity. Quinolone antibiotics (nalidixic acid, oxolinic acid and pefloxacin ) known to inhibit DNA replication were tested for their effects on these enzymes. DNA ligase activity was not affe...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Rusquet R,Bonhommet M,David JC

    更新日期:1984-06-29 00:00:00

  • Transport characteristics of 5-aminosalicylic acid into colonic epithelium: Involvement of sodium-coupled monocarboxylate transporter SMCT1-mediated transport system.

    abstract::5-Aminosalicylic acid (5-ASA) is conventionally used as a first line drug for inflammatory bowel disease (IBD). Because 5-ASA is well absorbed in the small intestine, very high dose of 5-ASA is required to deliver it to the large intestine which is a target site. Interestingly, 5-ASA is reported to be transported into...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yuri T,Kono Y,Fujita T

    更新日期:2020-04-09 00:00:00

  • Hairpin antisense oligonucleotides containing 2'-methoxynucleosides with base-pairing in the stem region at the 3'-end: penetration, localization, and Anti-HIV activity.

    abstract::Hairpin antisense oligodeoxyribonucleotides containing 2'-methoxynucleosides were more active in the micromolar concentration range than linear and DNA hairpin phosphorothioate oligonucleotides with the same sequence. Furthermore, the abilities of hairpin antisense and random hairpin phosphorothioate oligonucleotides ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kuwasaki T,Hosono K,Takai K,Ushijima K,Nakashima H,Saito T,Yamamoto N,Takaku H

    更新日期:1996-11-12 00:00:00

  • Blood group A glycolipid (Ax) with globo-series structure which is specific for blood group A1 erythrocytes: one of the chemical bases for A1 and A2 distinction.

    abstract::A new blood group A-active glycolipid fraction, termed Ax, showing a chromatographic mobility between Aa and Ab was found in blood group A1 erythrocytes but not in A2 erythrocytes. Ax was identified by its conversion to "globo H" by alpha-N-acetylgalactosaminidase and by 1H-NMR spectroscopy as GalNAc alpha l----3[Fuc ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Clausen H,Watanabe K,Kannagi R,Levery SB,Nudelman E,Arao-Tomono Y,Hakomori S

    更新日期:1984-10-30 00:00:00

  • Active transducin alpha subunit carries PDE6 to detergent-resistant membranes in rod photoreceptor outer segments.

    abstract::cGMP-Phosphodiesterase 6 (PDE6) is the central effector enzyme in the phototransduction system of vertebrate photoreceptors. We have recently found that PDE6 accumulates in a detergent-resistant membrane (DRM) fraction in response to excitation of bovine rod phototransduction system. Here, we studied the molecular mec...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Liu H,Seno K,Hayashi F

    更新日期:2003-03-28 00:00:00

  • Potent inhibitors of amyloid β fibrillization, 4,5-dianilinophthalimide and staurosporine aglycone, enhance degradation of preformed aggregates of mutant Notch3.

    abstract::Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is caused by mutations in human NOTCH3. We have recently reported that mutant Notch3 shows a greater propensity to form aggregates, and these aggregates resist degradation, leading to accumulation in the endoplasmic re...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Takahashi K,Adachi K,Kunimoto S,Wakita H,Takeda K,Watanabe A

    更新日期:2010-11-05 00:00:00

  • Inhibition of LPS binding to MD-2 co-receptor for suppressing TLR4-mediated expression of inflammatory cytokine by 1-dehydro-10-gingerdione from dietary ginger.

    abstract::Myeloid differentiation protein 2 (MD-2) is a co-receptor of toll-like receptor 4 (TLR4) for innate immunity. Here, we delineated a new mechanism of 1-dehydro-10-gingerdione (1D10G), one of pungent isolates from ginger (Zingiber officinale), in the suppression of lipopolysaccharide (LPS)-induced gene expression of inf...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Park SH,Kyeong MS,Hwang Y,Ryu SY,Han SB,Kim Y

    更新日期:2012-03-23 00:00:00

  • IL-33 signaling fuels outgrowth and metastasis of human lung cancer.

    abstract::IL-33 is a member of IL-1 superfamily that drives production of Th2-related cytokines. Recently, accumulating evidence suggest an involvement of IL-33 in carcinogenesis. Herein, we determine a close correlation of IL-33 expression and cancer progress in patients with non-small-cell lung cancer (NSCLC). Overexpression ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wang C,Chen Z,Bu X,Han Y,Shan S,Ren T,Song W

    更新日期:2016-10-21 00:00:00

  • The transcription factor MEF2C mediates cardiomyocyte hypertrophy induced by IGF-1 signaling.

    abstract::Myocyte enhancer factor 2C (MEF2C) plays an important role in cardiovascular development and is a key transcription factor for cardiac hypertrophy. Here, we describe MEF2C regulation by insulin-like growth factor-1 (IGF-1) and its role in IGF-1-induced cardiac hypertrophy. We found that IGF-1 addition to cultured rat ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Muñoz JP,Collao A,Chiong M,Maldonado C,Adasme T,Carrasco L,Ocaranza P,Bravo R,Gonzalez L,Díaz-Araya G,Hidalgo C,Lavandero S

    更新日期:2009-10-09 00:00:00

  • N-methyl-N-nitrosourea evidences cell cycle associated transient sequences in hepatic replicating DNA.

    abstract::Experiments of molecular hybridization were carried out with the aim of singling out DNA sequences which might be important in the hepatocarcinogenic action of N-methyl-N-nitrosourea. Results were obtained suggesting the synthesis of transitory DNA sequences during compensatory cell proliferation. ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kanduc D

    更新日期:1995-03-17 00:00:00

  • A reversible unfolding reaction of swine pepsin; implications for pepsinogen's folding mechanism.

    abstract::Above pH 6, swine pepsin undergoes a conformational change to a neutral form which has 80% of the secondary structure of the native protein. In contrast to native pepsin, this form of the enzyme can be reversibly unfolded by urea in a rapid, cooperative reaction. Since all of pepsin's sequence is present in its precur...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: McPhie P

    更新日期:1989-01-16 00:00:00

  • Inhibition of lysine-specific demethylase 1 by the acyclic diterpenoid geranylgeranoic acid and its derivatives.

    abstract::Lysine-specific demethylase 1 (LSD1) is upregulated in many cancers, especially neuroblastoma. We set out to explore whether geranylgeranoic acid (GGA) inhibits LSD1 activity by using recombinant human LSD1. GGA inhibited LSD1 activity with IC50 similar to that of the clinically used drug tranylcypromine. In human neu...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sakane C,Okitsu T,Wada A,Sagami H,Shidoji Y

    更新日期:2014-01-31 00:00:00

  • Leukotriene signaling via ALOX5 and cysteinyl leukotriene receptor 1 is dispensable for in vitro growth of CD34+CD38- stem and progenitor cells in chronic myeloid leukemia.

    abstract::Tyrosine kinase inhibitors targeting the BCR-ABL oncoprotein in chronic myeloid leukemia (CML) are remarkably effective inducing deep molecular remission in most patients. However, they are less effective to eradicate the leukemic stem cells (LSC), resulting in disease persistence. Therefore, there is great need to de...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Dolinska M,Piccini A,Wong WM,Gelali E,Johansson AS,Klang J,Xiao P,Yektaei-Karin E,Strömberg UO,Mustjoki S,Stenke L,Ekblom M,Qian H

    更新日期:2017-08-19 00:00:00

  • Calcium release associated with discharge of specific granule contents from human neutrophils.

    abstract::Neutrophil discharge of calcium into the ambient medium was measured, using an ion-sensitive electrode, after cells were stimulated with phorbol myristate acetate. Dose-dependent calcium efflux was observed over the same range of stimulus concentrations associated with specific granule marker release. Calcium efflux w...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Styrt B,Schwartz MA,Klempner MS

    更新日期:1988-08-15 00:00:00

  • Processing of the Hepatitis C virus precursor protein expressed in the methylotrophic yeast Pichia pastoris.

    abstract::The expression and processing of the Hepatitis C virus core protein (HCcAg) were analyzed in the methylotrophic yeast Pichia pastoris. Two proteins with 21 (p21) and 23 kDa (p23) were detected in immunoblot with a serum from a chronic carrier patient, as the major products for HCcAg. Both proteins, p21 and p23, produc...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Acosta-Rivero N,Musacchio A,Lorenzo L,Alvarez C,Morales J

    更新日期:2002-07-05 00:00:00