Regioselectivity of 7-O-methyltransferase of poplar to flavones.


:POMT-7, an O-methyltransferase from poplar (Populus deltoids) was used to modify a variety of flavonoid compounds. POMT-7 was able to transfer a methyl group to several flavonoids containing a C-7 hydroxyl group. However, POMT-7 showed a higher affinity toward flavonol and flavone such as apigenin, kaempferol, luteolin, and quercetin than flavanone and isoflavone. Based on comparison of HPLC retention times with authentic compounds and corresponding nuclear magnetic resonance spectroscopy data, the methylation position of the reaction products was determined to be at the hydroxyl group of C-7. Biotransformation kinetics indicated that the enzyme converted more than 80% of the apigenin, kaempferol, luteolin and quercetin substrates, which were added at concentration of 70 microM, into corresponding 7-methoxy compounds within 24 h.


J Biotechnol


Journal of biotechnology


Kim BG,Kim H,Hur HG,Lim Y,Ahn JH




Has Abstract


2006-11-01 00:00:00














  • Complete genome sequence of Streptomyces fulvissimus.

    abstract::The complete genome sequence of Streptomyces fulvissimus (DSM 40593), consisting of a linear chromosome with a size of 7.9Mbp, is reported. Preliminary data indicates that the chromosome of S. fulvissimus contains 32 putative gene clusters involved in the biosynthesis of secondary metabolites, two of them showing very...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Myronovskyi M,Tokovenko B,Manderscheid N,Petzke L,Luzhetskyy A

    更新日期:2013-10-10 00:00:00

  • Effective delivery of anti-miRNA DNA oligonucleotides by functionalized gold nanoparticles.

    abstract::MicroRNAs (miRNAs) are gaining recognition as essential regulators involved in many biological processes, and they are emerging as therapeutic targets for treating disease. Here, we introduce a method for effective delivery of anti-miRNA oligonucleotides (AMOs) using functionalized gold nanoparticles (AuNPs). To demon...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Kim JH,Yeom JH,Ko JJ,Han MS,Lee K,Na SY,Bae J

    更新日期:2011-09-20 00:00:00

  • Existence of beta-methylnorleucine in recombinant hirudin produced by Escherichia coli.

    abstract::A gene encoding for hirudin, a potent thrombin inhibitor, was expressed in Escherichia coli, which is the most widely used host. When the recombinant hirudin analog, CX-397, was overproduced by E. coli (600 mg l(-1)) in the absence of nutrient amino acids in the culture medium, the presence of two derivatives in the f...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Muramatsu R,Negishi T,Mimoto T,Miura A,Misawa S,Hayashi H

    更新日期:2002-02-14 00:00:00

  • Pellet morphology, culture rheology and lovastatin production in cultures of Aspergillus terreus.

    abstract::Pellet growth of Aspergillus terreus ATCC 20542 in submerged batch fermentations in stirred bioreactors was used to examine the effects of agitation (impeller tip speed u(t) of 1.01-2.71 ms(-1)) and aeration regimens (air or an oxygen-enriched mixture containing 80% oxygen and 20% nitrogen by volume) on the fungal pel...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Casas López JL,Sánchez Pérez JA,Fernández Sevilla JM,Rodríguez Porcel EM,Chisti Y

    更新日期:2005-03-02 00:00:00

  • Impact of sample processing on human airways microbial metagenomes.

    abstract::Whole metagenome shotgun sequencing provides information about the gene content and the composition of microbial communities provided that the processing of the samples does not introduce a methodology-driven bias. We tested the impact of DNA isolation and storage period on the metagenome profile. Deep throat swabs we...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Wiehlmann L,Pienkowska K,Hedtfeld S,Dorda M,Tümmler B

    更新日期:2017-05-20 00:00:00

  • The effect of the partition locus on plasmid stability and expression of a prolonged chemostat culture.

    abstract::The stability, copy number, and gene expression of the pBR322 plasmid containing the par-locus under prolonged cultivation were studied. In the initial stage of the experiment it was observed that the par-locus had a stabilization effect on plasmid maintenance. This observation was consistent with previously reported ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Weber AE,Yu P,San KY

    更新日期:1991-04-01 00:00:00

  • Improved fermentative production of the compatible solute ectoine by Corynebacterium glutamicum from glucose and alternative carbon sources.

    abstract::The cyclic amino acid ectoine is a compatible solute serving as a protective substance against osmotic stress. Ectoine finds various applications due to its moisturizing effect. To avoid the disadvantages of the prevailing so-called "bacterial milking ectoine production process" caused by the high salt concentration, ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Pérez-García F,Ziert C,Risse JM,Wendisch VF

    更新日期:2017-09-20 00:00:00

  • Genetic engineering of the Trichoderma reesei endoglucanase I (Cel7B) for enhanced partitioning in aqueous two-phase systems containing thermoseparating ethylene oxide--propylene oxide copolymers.

    abstract::Endoglucanases (endo-1,4-beta-D-glucan-4-glucanohydrolase, EC are industrially important enzymes. In this study endoglucanase I (EGI or Cel7B) of the filamentous fungi Trichoderma reesei has been genetically engineered to investigate the influence of tryptophan rich peptide extensions (tags) on partitioning i...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Collén A,Ward M,Tjerneld F,Stålbrand H

    更新日期:2001-05-04 00:00:00

  • Screening for improved isoprenoid biosynthesis in microorganisms.

    abstract::The production of isoprenoids in recombinant microbes for flavor & fragrance, pharmaceutical, agricultural or fuel applications is a booming research field. Isoprenoid extraction from natural resources and chemical synthesis is frequently neither ecological nor commercially profitable. However, recombinant microbes al...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Emmerstorfer-Augustin A,Moser S,Pichler H

    更新日期:2016-10-10 00:00:00

  • Cross-linked enzyme aggregates of Mung bean epoxide hydrolases: a highly active, stable and recyclable biocatalyst for asymmetric hydrolysis of epoxides.

    abstract::A highly active and stable cross-linked enzyme aggregates (CLEAs) of epoxide hydrolases (EHs) from Mung bean, which plays a crucial role in synthesis of valuable enantiopure diols, were successfully prepared and characterized. Under the optimum preparation conditions, the activity recovery of CLEAs recorded 92%. The C...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Yu CY,Li XF,Lou WY,Zong MH

    更新日期:2013-06-20 00:00:00

  • Structures of microbial communities found in anaerobic batch runs that produce methane from propionic acid--Seeded from full-scale anaerobic digesters above a certain threshold.

    abstract::The volatile fatty acid propionate inhibits anaerobic digestion during organic waste treatments. To examine potential microbial interactions that accelerate propionate oxidation, anaerobic digestion systems seeded with various types of anaerobic sludge were analyzed. Seed samples were collected from 10 different full-...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Kim W,Shin SG,Han G,Cho K,Hwang S

    更新日期:2015-11-20 00:00:00

  • The slow rotating centrifuge microscope NIZEMI--a versatile instrument for terrestrial hypergravity and space microgravity research in biology and materials science.

    abstract::NIZEMI (slow rotating centrifuge microscope) is a tool for optical investigations of small biological and non-biological specimens during variable accelerations. Two laboratory models for ground research designed for accelerations from 1 to 5 x g and 10 x g respectively are used for terrestrial research, especially i...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Friedrich UL,Joop O,Pütz C,Willich G

    更新日期:1996-06-27 00:00:00

  • Production of 5-methyluridine by immobilized thermostable purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus JTS 859.

    abstract::5-Methyluridine was produced continuously from thymine and inosine by immobilized enzymes, which consisted of thermostable purine nucleoside phosphorylase and thermostable pyrimidine nucleoside phosphorylase obtained from Bacillus stearothermophilus JTS 859. The process was carried out in a column reactor at 60 degree...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Hori N,Watanabe M,Sunagawa K,Uehara K,Mikami Y

    更新日期:1991-02-01 00:00:00

  • Strategy for producing new foot-and-mouth disease vaccines that display complex epitopes.

    abstract::Widely used inactivated vaccines for foot-and-mouth disease (FMD) induce protective immunity, but vaccine production plants and residual virus in the vaccine itself have been implicated in disease outbreaks. The structure of the FMD virion has been determined, and although much of the surface of the viral particle is ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: McKenna TS,Rieder E,Lubroth J,Burrage T,Baxt B,Mason PW

    更新日期:1996-01-26 00:00:00

  • Studies on the properties of Celluclast/Eudragit L-100 conjugate.

    abstract::A cellulase from Trichoderma reesei was immobilized on Eudragit L-100, a reversibly soluble polymer depending on the pH of the medium. The solubility of the modified cellulase was studied at different pH values. By changing the pH, the adsorption equilibrium of the derivatized proteins is switched towards the liquid p...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Dourado F,Bastos M,Mota M,Gama FM

    更新日期:2002-10-23 00:00:00

  • Enhancement of wound healing by chitosan/hyaluronan polyelectrolyte membrane loaded with glutathione: in vitro and in vivo evaluations.

    abstract::The inflammation of chronic wounds generally causes delaying their healing process. The present work aims to formulate a wound dressing polyelectrolyte membrane based on chitosan (Ch) and sodium hyaluronate (HA) loaded with glutathione (GSH). The membrane types (Ch/HA and Ch/HA/GSH) were examined by Fourier transform ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Tamer TM,Hassan MA,Valachová K,Omer AM,El-Shafeey MEA,Mohy Eldin MS,Šoltés L

    更新日期:2020-02-20 00:00:00

  • Continuous supply of glucose and glycerol enhances biotransformation of ricinoleic acid to (E)-11-(heptanoyloxy) undec-9-enoic acid in recombinant Escherichia coli.

    abstract::This study aimed at the development of biotransformation strategies with feeding of energy sources for bioconversion of ricinoleic acid to (E)-11-(heptanoyloxy) undec-9-enoic acid (11-HOUA), a key intermediate of brassylic acid, by recombinant Escherichia coli overexpressing an alcohol dehydrogenase from Micrococcus l...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Cho YH,Kim SJ,Kim HW,Kim JY,Gwak JS,Chung D,Kim KH,Park K,Park YC

    更新日期:2017-07-10 00:00:00

  • Enrichment of Plasticicumulans acidivorans at pilot-scale for PHA production on industrial wastewater.

    abstract::A PHA producing microbial culture dominated by Plasticicumulans acidivorans was enriched in a pilot plant using fermented wastewater from the Mars candy bar factory. The pilot plant comprised (1) anaerobic fermentation, (2) enrichment of a PHA-producing microbial community and (3) accumulation for maximization of the ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Tamisa J,Lužkov K,Jiang Y,van Loosdrecht MC,Kleerebezem R

    更新日期:2014-12-20 00:00:00

  • A simple statistical test of taxonomic or functional homogeneity using replicated microbiome sequencing samples.

    abstract::One important question in microbiome analysis is how to assess the homogeneity of the microbial composition in a given environment, with respect to a given analysis method. Do different microbial samples taken from the same environment follow the same taxonomic distribution of organisms, or the same distribution of fu...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Huson DH,Steel M,El-Hadidi M,Mitra S,Peter S,Willmann M

    更新日期:2017-05-20 00:00:00

  • Direct oligonucleotide synthesis onto super-paramagnetic beads.

    abstract::Super-paramagnetic beads (SPMB)s used for a variety of molecular diagnostic assays are prepared by attaching pre-synthesized oligonucleotides to the surface via a cumbersome and low efficient method of carbodiimide-mediated amide bond formation. To mainstream the process, we describe a novel procedure of direct oligon...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Jensen MA,Akhras MS,Fukushima M,Pourmand N,Davis RW

    更新日期:2013-09-20 00:00:00

  • Chemostat studies of bacteriophage M13 infected Escherichia coli JM109 for continuous ssDNA production.

    abstract::Steady state studies in a chemostat enable the control of microbial growth rate at defined reaction conditions. The effects of bacteriophage M13 infection on maximum growth rate of Escherichia coli JM109 were studied in parallel operated chemostats on a milliliter-scale to analyze the steady state kinetics of phage pr...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Kick B,Behler KL,Severin TS,Weuster-Botz D

    更新日期:2017-09-20 00:00:00

  • A flow cytometric study of hydrodynamic damage to mammalian cells.

    abstract::Flow cytometry has been used to study the mechanisms of damage to mammalian cells by hydrodynamic forces. Cell damage resulted from the stresses created both by bubble entrainment and by bubble bursting caused by vortex formation in highly agitated culture. Damage to the antigen molecules on the cell surface together ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: al-Rubeai M,Emery AN,Chalder S,Goldman MH

    更新日期:1993-11-01 00:00:00

  • A repeat-batch membrane bioreactor with a phase inversion for the desaturation of isopropyl palmitate by a mutant Rhodococcus strain.

    abstract::A repeat-batch membrane bioreactor was constructed for the novel bioconversion of isopropyl hexadecanoate to isopropyl cis-6-hexadecenoate by a Rhodococcus mutant. The addition of glutamate, thiamine, and MgSO(4) was very effective in improving not only the rate and yield of the bioconversion but also the maintenance ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Koike K,Takeuchi K,Mino H,Takaiwa M,Tohoh T,Tadokoro T,Tsutoh K,Ito S

    更新日期:2000-06-23 00:00:00

  • Synthesis, characterization of penicillin G capped silver nanoconjugates to combat β-lactamase resistance in infectious microorganism.

    abstract::In the present study, a novel strategy was adopted to synthesize, β lactamase resistant penicillin G molecules by using the unique properties of silver nanoparticles. Ascorbic acid-stabilized spherical monodispersed silver nanoparticles were prepared by a simple chemical reaction. The formation processes of the silver...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Ahmed V,Kumar J,Kumar M,Chauhan MB,Vij M,Ganguli M,Chauhan NS

    更新日期:2013-02-20 00:00:00

  • Effect of oligosaccharide elicitors on bacitracin A production and evidence of transcriptional level control.

    abstract::The role of oligosaccharides as biotic elicitors for the enhanced production of antibiotics and enzymes has been established in recent years. These findings could have significant promise for the pharmaceutical and biotechnology industries. Before the elicitors could be used at large-scale, reproducible production and...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Murphy T,Roy I,Harrop A,Dixon K,Keshavarz T

    更新日期:2007-09-30 00:00:00

  • Directed enzyme evolution and selections for catalysis based on product formation.

    abstract::Enzyme engineering by molecular modelling and site-directed mutagenesis can be remarkably efficient. Directed enzyme evolution appears as a more general strategy for the isolation of catalysts as it can be applied to most chemical reactions in aqueous solutions. Selections, as opposed to screening, allow the simultane...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Jestin JL,Kaminski PA

    更新日期:2004-09-30 00:00:00

  • Cloning and functional expression of glucose dehydrogenase complex of Burkholderia cepacia in Escherichia coli.

    abstract::The thermostable glucose dehydrogenase (GDH) from Burkholderia cepacia sp. SM4 is composed of a catalytic subunit (alpha), an electron transfer subunit (beta), and a small gamma subunit of unknown function. We cloned a 1428-nucleotide gene encoding the beta subunit located immediately downstream of the alpha subunit. ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Tsuya T,Ferri S,Fujikawa M,Yamaoka H,Sode K

    更新日期:2006-05-17 00:00:00

  • Site-specific tetrameric streptavidin-protein conjugation using sortase A.

    abstract::Streptavidin is tetrameric protein which has tight and specific biotin binding affinity, and streptavidin modification of proteins or small molecules is widely used for biotechnology tool. Here, we demonstrate site-specific streptavidin-protein conjugation using enzymes. We focused on sortase A, a transpeptidase from ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Matsumoto T,Sawamoto S,Sakamoto T,Tanaka T,Fukuda H,Kondo A

    更新日期:2011-03-10 00:00:00

  • Development of a FIA system with immobilized enzymes for specific post-column detection of purine bases and their nucleosides separated by HPLC column.

    abstract::A sensitive and highly selective method for the simultaneous determination of purine bases and their nucleosides is proposed. An amperometric flow-injection system with the two immobilized enzyme reactors (guanase immobilized reactor and purine nucleoside phosphorylase/xanthine oxidase co-immobilized reactor) is used ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Yao T,Matsumoto Y,Wasa T

    更新日期:1990-04-01 00:00:00

  • The membrane bioreactor with coenzyme recycling system.

    abstract::Much attention has been paid to membrane bioreactors, especially to the newly developed charged membrane bioreactor (CMBR) for recycling the native form of the coenzyme NAD(P)H. Charged membranes with anionic groups effectively retained the free nicotinamide coenzyme due to the electrostatic repulsion between membrane...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Ikemi M,Ishimatsu Y

    更新日期:1990-05-01 00:00:00