Regioselectivity of 7-O-methyltransferase of poplar to flavones.

Abstract:

:POMT-7, an O-methyltransferase from poplar (Populus deltoids) was used to modify a variety of flavonoid compounds. POMT-7 was able to transfer a methyl group to several flavonoids containing a C-7 hydroxyl group. However, POMT-7 showed a higher affinity toward flavonol and flavone such as apigenin, kaempferol, luteolin, and quercetin than flavanone and isoflavone. Based on comparison of HPLC retention times with authentic compounds and corresponding nuclear magnetic resonance spectroscopy data, the methylation position of the reaction products was determined to be at the hydroxyl group of C-7. Biotransformation kinetics indicated that the enzyme converted more than 80% of the apigenin, kaempferol, luteolin and quercetin substrates, which were added at concentration of 70 microM, into corresponding 7-methoxy compounds within 24 h.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Kim BG,Kim H,Hur HG,Lim Y,Ahn JH

doi

10.1016/j.jbiotec.2006.04.019

subject

Has Abstract

pub_date

2006-11-01 00:00:00

pages

241-7

issue

2

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(06)00333-6

journal_volume

126

pub_type

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