Epidermal growth factor receptor expression regulates proliferation in the postnatal rat retina.


:Epidermal growth factor (EGF) is known to promote proliferation of both retinal progenitors and Muller glia in vitro, but several questions remain concerning an in vivo role for this factor. In this study, we investigated whether the EGF receptor (EGFR) is necessary for the maintenance of normal levels of progenitor and Muller glial proliferation in vivo. Here, we show that (1) mice with homozygous deletion of the Egfr gene have reduced proliferation in late stages of retinal histogenesis, (2) EGF is mitogenic for Müller glia in vivo during the first two postnatal weeks in the rodent retina, (3) the effectiveness of EGF as a Müller glial mitogen declines in parallel with the decline in EGFR expression as the retina matures, and (4) following damage to the retina from continuous light exposure, EGFR expression is up-regulated in Müller glia to levels close to those in the neonatal retina, resulting in a renewed mitotic response to EGF. Together with previous results from other studies, these data indicate that the downregulation of a growth factor receptor is one mechanism by which glial cells maintain mitotic quiescence in the mature nervous system.






Close JL,Liu J,Gumuscu B,Reh TA




Has Abstract


2006-08-01 00:00:00














  • The CpG island shore of the GLT-1 gene acts as a methylation-sensitive enhancer.

    abstract::Astrocytic lineage commitment and brain region-dependent specialization of glia are partly ascribed to epigenetic processes. Clearance of glutamate is an essential task, which astrocytes assume in a temporal-spatial fashion by distinct glutamate transporter expression. Glutamate transporter subtype 1 (GLT-1) is predom...


    pub_type: 杂志文章


    authors: Perisic T,Holsboer F,Rein T,Zschocke J

    更新日期:2012-09-01 00:00:00

  • Nogo-A inhibits vascular regeneration in ischemic retinopathy.

    abstract::Nogo-A is a potent glial-derived inhibitor of axon growth in the injured CNS and acts as a negative regulator of developmental angiogenesis by inhibiting vascular endothelial cell migration. However, its function in pathological angiogenesis has never been studied after ischemic injury in the CNS. Using the mouse mode...


    pub_type: 杂志文章


    authors: Joly S,Dejda A,Rodriguez L,Sapieha P,Pernet V

    更新日期:2018-10-01 00:00:00

  • Cellular distribution of superoxide dismutases in the rat CNS.

    abstract::Superoxide dismutase (SOD) is considered to be a major factor in protection of nervous tissue against excitotoxic and ischemic/hypoxic lesion. Controversial reports about the localization of SOD after such an insult prompted us to re-investigate immunocytochemically the localization of the enzyme in the brain and spin...


    pub_type: 杂志文章


    authors: Lindenau J,Noack H,Possel H,Asayama K,Wolf G

    更新日期:2000-01-01 00:00:00

  • Neuregulins and erbB receptor expression in adult human oligodendrocytes.

    abstract::We have previously reported that neonatal rat oligodendrocytes (OLGs) express and secrete neuregulins (NRGs) (Raabe et al., 1997). This laboratory has also shown that NRGs stimulate the differentiation of neonatal rat OLGs and that these cells express the erbB receptors for NRGs (Raabe et al., 1997). In this study, we...


    pub_type: 杂志文章


    authors: Deadwyler GD,Pouly S,Antel JP,Devries GH

    更新日期:2000-12-01 00:00:00

  • Intermediate filament protein synemin is present in human reactive and malignant astrocytes and associates with ruffled membranes in astrocytoma cells.

    abstract::Synemin, a very unique type VI intermediate filament (IF) protein, exhibits alternative splice variants termed alpha and beta. Unlike other IF proteins, synemin binds to actin-associated proteins, including alpha-actinin, vinculin, and alpha-dystrobrevin. Our previous work has demonstrated the presence of synemin in d...


    pub_type: 杂志文章


    authors: Jing R,Pizzolato G,Robson RM,Gabbiani G,Skalli O

    更新日期:2005-04-15 00:00:00

  • Natural and induced cytotoxicity of oligodendrocytes by microglia is inhibitable by TGF beta.

    abstract::Blood macrophages and brain macrophages (microglia) have been implicated in demyelination and destruction of the oligodendrocyte in multiple sclerosis (MS), a disease affecting primarily white matter of the central nervous system (CNS). In this study, we demonstrate that at high effector to target cell ratios, normal ...


    pub_type: 杂志文章


    authors: Merrill JE,Zimmerman RP

    更新日期:1991-01-01 00:00:00

  • Bioenergetic regulation of microglia.

    abstract::Microglia have diverse actions, ranging from synapse pruning in development to cytotoxic effects in disease. Brain energy metabolism and substrate availability vary under normal and disease states, but how these variations influence microglial function is relatively unknown. Microglia, like most other cell types, expr...


    pub_type: 杂志文章,评审


    authors: Ghosh S,Castillo E,Frias ES,Swanson RA

    更新日期:2018-06-01 00:00:00

  • Astroglia contribute to the pathogenesis of spinocerebellar ataxia Type 1 (SCA1) in a biphasic, stage-of-disease specific manner.

    abstract::Spinocerebellar ataxia type 1 (SCA1) is a fatal, dominantly inherited neurodegenerative disease caused by the expansion of CAG repeats in the Ataxin-1 (ATXN1) gene. SCA1 is characterized by balance and coordination deficits due to the predominant loss of Purkinje neurons in the cerebellum. We previously demonstrated t...


    pub_type: 杂志文章


    authors: Kim JH,Lukowicz A,Qu W,Johnson A,Cvetanovic M

    更新日期:2018-09-01 00:00:00

  • beta-catenin mediates insulin-like growth factor-I actions to promote cyclin D1 mRNA expression, cell proliferation and survival in oligodendroglial cultures.

    abstract::By promoting cell proliferation, survival and maturation insulin-like growth factor (IGF)-I is essential to the normal growth and development of the central nervous system. It is clear that IGF-I actions are primarily mediated by the type I IGF receptor (IGF1R), and that phosphoinositide 3 (PI3)-Akt kinases and MAP ki...


    pub_type: 杂志文章


    authors: Ye P,Hu Q,Liu H,Yan Y,D'ercole AJ

    更新日期:2010-07-01 00:00:00

  • The enteric glia: identity and functions.

    abstract::Enteric glial cells were first described at the end of the 19th century, but they attracted more interest from researchers only in the last decades of the 20th. Although, they have a different embryological origin, the enteric GLIA share many characteristics with astrocytes, the main glial cell type of the central ner...


    pub_type: 杂志文章,评审


    authors: Coelho-Aguiar Jde M,Bon-Frauches AC,Gomes AL,Veríssimo CP,Aguiar DP,Matias D,Thomasi BB,Gomes AS,Brito GA,Moura-Neto V

    更新日期:2015-06-01 00:00:00

  • Ultrastructural relationships between noradrenergic nerve fibers and non-neuronal elements in the rat cerebral cortex.

    abstract::Pharmacological and biochemical data suggest that noradrenaline (NA)-containing fibers not only regulate the activity of cortical neurons but also influence the functional state of non-neuronal elements. In the present study, immunocytochemistry with an antiserum against NA, followed by silver-gold intensification of ...


    pub_type: 杂志文章


    authors: Paspalas CD,Papadopoulos GC

    更新日期:1996-06-01 00:00:00

  • Mice lacking NT-3, and its receptor TrkC, exhibit profound deficiencies in CNS glial cells.

    abstract::Neurotrophin-3 (NT-3) and its receptor TrkC are known to be important for neuronal survival. More recently, NT-3 has been implicated as playing a role in oligodendrocyte (OL) proliferation and survival in vitro. Examination of NT-3 and TrkC knockout mice revealed a reduction in NT-3-dependent neurons. To date, no stud...


    pub_type: 杂志文章


    authors: Kahn MA,Kumar S,Liebl D,Chang R,Parada LF,De Vellis J

    更新日期:1999-04-01 00:00:00

  • NMDA receptor couples Rac1-GEF Tiam1 to direct oligodendrocyte precursor cell migration.

    abstract::Oligodendrocyte precursor cells (OPCs) originate from restricted regions of the brain and migrate into the developing white matter, where they differentiate into oligodendrocytes and myelinate axons in the central nervous system (CNS). The molecular mechanisms that orchestrate these long distance trips of OPCs to popu...


    pub_type: 杂志文章


    authors: Xiao L,Hu C,Yang W,Guo D,Li C,Shen W,Liu X,Aijun H,Dan W,He C

    更新日期:2013-12-01 00:00:00

  • Brain-immune connection: immuno-regulatory properties of CNS-resident cells.

    abstract::Even though the immune privileged status of the central nervous system (CNS) limits access of systemic immune cells through the blood brain barrier (BBB), an immune response can occur in this compartment with or without major breach of the BBB. In this review, we consider properties of resident cells of the CNS, that ...


    pub_type: 杂志文章,评审


    authors: Becher B,Prat A,Antel JP

    更新日期:2000-02-15 00:00:00

  • Astrocytes protect neurons from neurotoxic injury by serum glutamate.

    abstract::Serum is used widely for culturing neurons and glial cells, and is thought to provide essential, albeit undefined, factors such as hormones, growth factors, and trace elements that promote the growth of cells in vitro. Moreover, serum can have profound effects on cell proliferation, differentiation, and cell morpholog...


    pub_type: 杂志文章


    authors: Ye ZC,Sontheimer H

    更新日期:1998-03-01 00:00:00

  • Organization of glial cells in the adult sea cucumber central nervous system.

    abstract::The nervous system of echinoderms has long been considered too unique to be directly comparable to the nervous system of other Deuterostomia. Using two novel monoclonal antibodies in combination with epifluorescence, confocal, and electron microscopy, we demonstrate here that the central nervous system of the sea cucu...


    pub_type: 杂志文章


    authors: Mashanov VS,Zueva OR,Garcia-Arraras JE

    更新日期:2010-10-01 00:00:00

  • Calcium dependence of serotonin-evoked conductance in C6 glioma cells.

    abstract::Whole-cell membrane currents and imaging of intracellular calcium concentrations ([Ca2+]i) were used to investigate the role of calcium in a response to serotonin of C6 glioma cells. Activation of a high-affinity serotonin receptor induced a transient rise in calcium concentration in these cells and activated a predom...


    pub_type: 杂志文章


    authors: Manor D,Moran N,Segal M

    更新日期:1992-01-01 00:00:00

  • Neurotrophic and migratory properties of an olfactory ensheathing cell line.

    abstract::Olfactory ensheathing cells (OECs) are a unique type of macroglia required for normal olfactory axonal regeneration throughout the lifetime of an individual. Recent evidence in the literature suggests that OECs transplanted into injured spinal cords may facilitate axonal regeneration. In this study, we evaluated the n...


    pub_type: 杂志文章


    authors: Boruch AV,Conners JJ,Pipitone M,Deadwyler G,Storer PD,Devries GH,Jones KJ

    更新日期:2001-03-01 00:00:00

  • Matrix metalloproteinase-9 interplays with the IGFBP2-IGFII complex to promote cell growth and motility in astrocytomas.

    abstract::Insulin-like growth factor II (IGFII) acts as a potent mitogen for several tumor types and has been reported to positively influence astrocytoma cell growth and motility. In the central nervous system, IGFII bioavailability is mainly modulated by insulin-like growth factor binding protein 2 (IGFBP2), which sequestrate...


    pub_type: 杂志文章


    authors: Rorive S,Berton A,D'haene N,Takacs CN,Debeir O,Decaestecker C,Salmon I

    更新日期:2008-11-15 00:00:00

  • Leukemia inhibitory factor promotes oligodendrocyte survival after spinal cord injury.

    abstract::Injury to the mammalian spinal cord is accompanied by a delayed, secondary wave of oligodendrocyte apoptosis that arises several days after the initial injury. A strong candidate to support oligodendrocyte survival after spinal cord injury is the pleiotropic cytokine, leukemia inhibitory factor (LIF). In vitro, LIF po...


    pub_type: 杂志文章


    authors: Kerr BJ,Patterson PH

    更新日期:2005-07-01 00:00:00

  • Isoform-specific expression of sodium channels in astrocytes in vitro: immunocytochemical observations.

    abstract::The expression of sodium channel alpha-subunit isoforms in astrocytes cultured from P-0 rat spinal cord and P-7 rat optic nerve was examined utilizing immunocytochemical methods with antibodies generated against conserved and isoform-specific amino acid sequences of the rat brain sodium channel. In spinal cord culture...


    pub_type: 杂志文章


    authors: Black JA,Westenbroek R,Minturn JE,Ransom BR,Catterall WA,Waxman SG

    更新日期:1995-06-01 00:00:00

  • Cleavage of membrane-associated ICAM-1 from astrocytes: involvement of a metalloprotease.

    abstract::Disease of the central nervous system (CNS) with immune-mediated pathogenesis is frequently associated with enhanced expression of intercellular adhesion molecule-1 (ICAM-1) on resident glial cells, including astrocytes. Recently, a soluble form of ICAM-1 (sICAM-1) has been demonstrated within the CNS and cerebrospina...


    pub_type: 杂志文章


    authors: Lyons PD,Benveniste EN

    更新日期:1998-02-01 00:00:00

  • Distribution of Aquaporin 4 in rodent spinal cord: relationship with astrocyte markers and chondroitin sulfate proteoglycans.

    abstract::Water balance between cells and extracellular compartments is essential for proper functioning of the central nervous system, as demonstrated by its perturbations in pathological conditions. Aquaporin 4 (AQP4) is the predominant water channel in brain and spinal cord, where it is present mainly on astrocytic endfeet c...


    pub_type: 杂志文章


    authors: Vitellaro-Zuccarello L,Mazzetti S,Bosisio P,Monti C,De Biasi S

    更新日期:2005-08-01 00:00:00

  • Peripheral astrocyte processes: monitoring by selective immunostaining for the actin-binding ERM proteins.

    abstract::Astrocytes extend thin lamellate processes in the neuropil, in particular around synapses, where they can modulate synaptic function or mediate glial-neuronal communication. Previous studies have shown that these lamellate perisynaptic processes change their shape in response to neuronal activity, but the underlying m...


    pub_type: 杂志文章


    authors: Derouiche A,Frotscher M

    更新日期:2001-12-01 00:00:00

  • Downregulation of miR-7116-5p in microglia by MPP+ sensitizes TNF-α production to induce dopaminergic neuron damage.

    abstract::Activation of microglia resulting in exacerbated inflammation expression plays an important role in degeneration of dopaminergic (DA) neurons in the pathogenesis of Parkinson's disease (PD). However, how this enhanced inflammation is induced in microglia remains largely unclear. Here, in the mouse PD model induced by ...


    pub_type: 杂志文章


    authors: He Q,Wang Q,Yuan C,Wang Y

    更新日期:2017-08-01 00:00:00

  • The voltage-gated potassium channel Kv1.3 is required for microglial pro-inflammatory activation in vivo.

    abstract::Microglia show a rich repertoire of activation patterns regulated by a complex ensemble of surface ion channels, receptors, and transporters. We and others have investigated whether microglia vary their K+ channel expression as a means to achieve functional diversity. However, most of the prior studies were conducted ...


    pub_type: 杂志文章


    authors: Di Lucente J,Nguyen HM,Wulff H,Jin LW,Maezawa I

    更新日期:2018-09-01 00:00:00

  • Thioredoxin reductase and glutathione synthesis is upregulated by t-butylhydroquinone in cortical astrocytes but not in cortical neurons.

    abstract::The electron donors glutathione and thioredoxin play many vital roles in the mechanisms of cells to cope with oxidative stress. Critical to such antioxidant functions are the ability to synthesize glutathione and keep it reduced via glutathione reductase and the ability to reduce oxidized-thioredoxin via thioredoxin r...


    pub_type: 杂志文章


    authors: Eftekharpour E,Holmgren A,Juurlink BH

    更新日期:2000-09-01 00:00:00

  • NADPH oxidase and aging drive microglial activation, oxidative stress, and dopaminergic neurodegeneration following systemic LPS administration.

    abstract::Parkinson's disease is characterized by a progressive degeneration of substantia nigra (SN) dopaminergic neurons with age. We previously found that a single systemic lipopolysaccharide (LPS, 5 mg/kg, i.p.) injection caused a slow progressive loss of tyrosine hydroxylase immunoreactive (TH+IR) neurons in SN associated ...


    pub_type: 杂志文章


    authors: Qin L,Liu Y,Hong JS,Crews FT

    更新日期:2013-06-01 00:00:00

  • Defined astrocytic expression of human amyloid precursor protein in Tg2576 mouse brain.

    abstract::Transgenic Tg2576 mice expressing human amyloid precursor protein (hAPP) with the Swedish mutation are among the most frequently used animal models to study the amyloid pathology related to Alzheimer's disease (AD). The transgene expression in this model is considered to be neuron-specific. Using a novel hAPP-specific...


    pub_type: 杂志文章


    authors: Heiland T,Zeitschel U,Puchades MA,Kuhn PH,Lichtenthaler SF,Bjaalie JG,Hartlage-Rübsamen M,Roßner S,Höfling C

    更新日期:2019-02-01 00:00:00

  • Comparative biochemical, morphological, and immunocytochemical studies between C-6 glial cells of early and late passages and advanced passages of glial cells derived from aged mouse cerebral hemispheres.

    abstract::We have used C6 glial cells (2B clone), early and late passage, as well as advanced passages (8-17) of glial cells derived from aged (18-month-old) mouse cerebral hemispheres (MACH), as model systems for studying glial properties. In this study passages 20-24 were considered "early" and passages 73-90 were considered ...


    pub_type: 杂志文章


    authors: Lee K,Kentroti S,Billie H,Bruce C,Vernadakis A

    更新日期:1992-01-01 00:00:00