Aberrant methylation of secreted apoptosis-related protein 2 (SARP2) in pure pancreatic juice in diagnosis of pancreatic neoplasms.

Abstract:

OBJECTIVE:Secreted apoptosis-related protein (SARP) families are considered to counteract the oncogenic Wnt signaling pathway, and inactivation of this gene may aid cancer development and progression. Recently, the aberrant methylation of SARP2 was detected frequently in pancreatic carcinoma (PCa) tissue, but not in normal pancreatic tissue. We evaluated the hypermethylation of SARP2 in pure pancreatic juices (PPJ) aspirated endoscopically from patients with PCa, intraductal papillary mucinous neoplasm of the pancreas (IPMN), chronic pancreatitis (CP), and a control group who were consequently free of pancreatic diseases according to the differential diagnosis of PCa. METHODS:We investigated the aberrant methylation of SARP2 in 9 human PCa cell lines and in the PPJ samples from 33 patients with PCa, 20 with IPMN, 19 with CP, and 10 control patients. DNAs extracted from not only sediment, but also the supernatant of the PPJ and PCa cell lines were treated with sodium bisulfite and analyzed by methylation-specific polymerase chain reaction (PCR) (MSP). Moreover, real-time MSP was also performed for the melting curve analysis and the quantitative analysis of SARP2 in the PPJ. RESULTS:The incidence of the aberrant methylation of SARP2 using MSP was 8/9 (89%) in PCa cell lines, 26/33 (79%) in the PPJ with PCa, and 17/20 (85%) with IPMN. However, it was only 1/19 (5%) in the PPJ with CP, and 0/10 (0%) in the PPJ of the control patients, respectively. The incidences of aberrant methylation of SARP2 in the PPJ with PCa and IPMN were significantly higher than that in the PPJ with CP (P < 0.001, P < 0.001). Melting curve analysis by real-time MSP revealed that the incidences of aberrant methylation of SARP2 in PPJ was 28/33 (85%) with PCa, 9/11 (82%) with the malignant group of IPMN, 5/9 (56%) with the benign group of IPMN and 5/19 (26%) with CP. In this analysis, there were significant differences between PCa and CP (P < 0.001), and between the malignant group of IPMN and CP (P < 0.005). In the quantitative analysis by real-time MSP with a suitable cut-off value, the incidences of aberrant methylation of SARP2 in the PPJ with PCa, the malignant group of IPMN, the benign group of IPMN, and CP were 19/33 (58%), 6/11 (55%), 3/9 (33%), and 2/19 (11%), respectively. The incidence of the aberrant methylation of SARP2 in the PPJ was significantly different between PCa and CP and between the malignant group of IPMN and CP (P < 0.005 and P < 0.05, respectively). CONCLUSIONS:Hypermethylation of SARP2 in the PPJ may be a highly sensitive and useful marker for the detection of pancreatic neoplasms, including PCa and the malignant group of IPMN.

journal_name

Pancreas

journal_title

Pancreas

authors

Watanabe H,Okada G,Ohtsubo K,Yao F,Jiang PH,Mouri H,Wakabayashi T,Sawabu N

doi

10.1097/01.mpa.0000221617.89376.38

subject

Has Abstract

pub_date

2006-05-01 00:00:00

pages

382-9

issue

4

eissn

0885-3177

issn

1536-4828

pii

00006676-200605000-00008

journal_volume

32

pub_type

杂志文章

相关文献

PANCREAS文献大全
  • Peptide YY inhibits nutrient-, hormonal-, and vagally-stimulated pancreatic exocrine secretion.

    abstract::Peptide YY (PYY) is a recently isolated gut peptide that is found primarily in the mucosal endocrine cells of the terminal ileum, colon, and rectum of several mammalian species, including humans. The purpose of this study was to characterize the effect of PYY on pancreatic exocrine secretion in six conscious dogs prep...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-198707000-00013

    authors: Lluis F,Gomez G,Fujimura M,Greeley GH Jr,Thompson JC

    更新日期:1987-01-01 00:00:00

  • Conversion of human pancreatic acinar cells toward a ductal-mesenchymal phenotype and the role of transforming growth factor β and activin signaling.

    abstract:OBJECTIVE:Epithelial-mesenchymal transition may interfere with the differentiation of cultured pancreatic acinar cells toward endocrine cells. Therefore, it will be important to investigate into detail the reprogramming of human pancreatic acinar cells toward a mesenchymal phenotype: the association with acinoductal tr...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0000000000000154

    authors: De Waele E,Wauters E,Ling Z,Bouwens L

    更新日期:2014-10-01 00:00:00

  • Endoscopic stent therapy for patients with chronic pancreatitis: results from a prospective follow-up study.

    abstract:OBJECTIVES:Obstruction of the pancreatic duct is a common feature of chronic pancreatitis and often requires interventional therapy. The current prospective study investigated clinical success in 19 patients after initial endoscopic retrograde pancreaticography and relapse rates during a 2-year follow-up period. METHO...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/mpa.0b013e3180325ba6

    authors: Weber A,Schneider J,Neu B,Meining A,Born P,Schmid RM,Prinz C

    更新日期:2007-04-01 00:00:00

  • Acute Pancreatitis and Use of Pancreatitis-Associated Drugs: A 10-Year Population-Based Cohort Study.

    abstract:OBJECTIVES:To assess the use of acute pancreatitis (AP)-associated drugs in patients with AP, the relation between sales of these drugs and the incidence of AP, and the potential impact on AP severity and recurrence. METHODS:All patients with incident AP between 2003 and 2012, in a well-defined area, were retrospectiv...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0000000000000406

    authors: Bertilsson S,Kalaitzakis E

    更新日期:2015-10-01 00:00:00

  • Involvement of endogenous prostaglandins in pancreatic endocrine and exocrine secretion in dog pancreas.

    abstract::The involvement of endogenous prostaglandins (PGs) in pancreatic endocrine and exocrine secretion was investigated, using the isolated and perfused dog pancreas. Spontaneous production of both PGE2 and 6-keto-PGF1 alpha was recorded in venous effluent. Prostaglandin production increased following stimulation with both...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-198912000-00008

    authors: Kuroda T,Sodeyama H,Hanazaki K,Horigome N,Kajikawa S,Yamagishi K,Horiuchi A,Iwatsuki K,Chiba S,Homma T

    更新日期:1989-01-01 00:00:00

  • Prior History of Pancreatitis Accelerates the Development of Pancreatic Adenocarcinoma.

    abstract:OBJECTIVES:Presentation of pancreatic adenocarcinoma (PC) as acute pancreatitis (AP), association of chronic pancreatitis (CP) with PC, and role of inflammation in PC carcinogenesis are well recognized. We hypothesized that inflammatory changes associated with remote history of AP (≥2 years before PC diagnosis) would r...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0000000000001160

    authors: Phillips AE,Shah N,Borhani AA,Yadav D,Brand RE

    更新日期:2018-11-01 00:00:00

  • Apigenin Decreases Acinar Cell Damage in Pancreatitis.

    abstract:OBJECTIVE:Chronic pancreatitis is the consequence of multiple episodes of recurrent acute pancreatitis (RAP). We hypothesized that apigenin can minimize the sequelae of RAP by limiting acinar cells' proinflammatory signaling pathways. METHODS:AR42J acinar cells were treated in vitro with transforming growth factor β (...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0000000000001310

    authors: Mrazek AA,Bhatia V,Falzon M,Spratt H,Chao C,Hellmich MR

    更新日期:2019-05-01 00:00:00

  • Three female familial cases of solid pseudopapillary tumors with a protease serine 1 gene mutation.

    abstract::Solid pseudopapillary tumors (SPTs) are a rare pancreatic neoplastic lesion. Familial aggregation has not been reported in this disease. The objectives of this study were to report the history, clinicopathological features, and gene mutations of 3 familial cases of SPT. Three female cases of SPT presented in 1 family....

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0b013e3182554276

    authors: Gou S,Yu J,Wang C,Liu T,Cui P,Li X

    更新日期:2013-01-01 00:00:00

  • Successful resection of a liver metastasis from AFP-producing pancreatic cancer resulting in long-term survival: a case report and review of literature.

    abstract::The prognosis of patients with alpha-fetoprotein (AFP)-producing pancreatic cancer is generally very poor. We, however, present a case of a 33-year-old man who survived AFP-producing pancreatic cancer and its metachronous liver metastasis. The patient had undergone a curative total pancreatectomy for AFP-producing pan...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/01.mpa.0000179727.15716.82

    authors: Natsume T,Watanabe Y,Maruyama T,Tanaka H,Sunouchi K,Tohma T,Noju M,Muto Y,Tohnosu N,Uehara T,Shimizu S,Ochiai T,Nabeya Y

    更新日期:2005-11-01 00:00:00

  • Tumor angiogenesis in chronic pancreatitis and pancreatic adenocarcinoma: impact of K-ras mutations.

    abstract::Chronic pancreatitis (CP) is one condition in which epidemiologic studies have demonstrated a definite association with pancreatic adenocarcinoma (PAC). The pathophysiologic and molecular events that either predispose to the development of, or potentiate the growth of, PAC are unknown. Mutation of the codon 12 K-ras g...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-200004000-00005

    authors: Banerjee SK,Zoubine MN,Mullick M,Weston AP,Cherian R,Campbell DR

    更新日期:2000-04-01 00:00:00

  • Chronic Asymptomatic Pancreatic Hyperenzymemia: A Long-term Follow-up.

    abstract:OBJECTIVES:Chronic asymptomatic pancreatic hyperenzymemia (CAPH) was described as a benign disease. However, we already described clinically relevant findings requiring surgery or follow-up in half of the subjects. The aim of this study was to evaluate the long-term outcome of CAPH in terms of symptoms and evolution to...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0000000000001272

    authors: Amodio A,De Marchi G,Granato A,de Pretis N,Gabbrielli A,Manfredi R,Pozzi Mucelli R,Frulloni L

    更新日期:2019-04-01 00:00:00

  • Prevalence and Associated Factors of Abdominal Pain and Disability at 1-Year Follow-up After an Attack of Acute Pancreatitis.

    abstract:OBJECTIVE:The aim of the study was to report the prevalence and predictors of abdominal pain and disability 1 year after an acute pancreatitis (AP) attack. METHODS:Patients were prospectively enrolled between December 2012 and April 2016. Enrolled subjects were contacted at a median of 13 months after enrollment. Mult...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0000000000001434

    authors: Gougol A,Machicado JD,Matta B,Paragomi P,Pothoulakis I,Slivka A,Whitcomb DC,Yadav D,Papachristou GI

    更新日期:2019-11-01 00:00:00

  • Suppression of human pancreatic carcinoma cell growth and invasion by epigallocatechin-3-gallate.

    abstract:INTRODUCTION:The consumption of green tea is associated with a lower risk of several types of human carcinomas. A number of studies have focused on the possible mechanisms of cancer prevention by tea extracts, especially polyphenols such as epigallocatechin-3-gallate (EGCG). AIMS AND METHODOLOGY:Green tea-derived EGCG...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-200207000-00012

    authors: Takada M,Nakamura Y,Koizumi T,Toyama H,Kamigaki T,Suzuki Y,Takeyama Y,Kuroda Y

    更新日期:2002-07-01 00:00:00

  • Effects of parathyroid hormone on pancreatic exocrine secretion.

    abstract::The role of parathyroid hormone (PTH) and calcium on pancreatic exocrine secretion were observed using sham-operated and parathyroidectomized dogs. First, exocrine secretion of the pancreas stimulated with secretin and cholecystokinin octapeptide (CCK-8) was examined in vivo 3 weeks after parathyroidectomy. Secondly, ...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-199311000-00010

    authors: Kuroda T,Shiohara E,Haba Y,Kaneko G,Hanazaki K,Kajikawa S,Iida F

    更新日期:1993-11-01 00:00:00

  • Laparoscopic management of severe acute pancreatitis.

    abstract:INTRODUCTION:Severe acute pancreatitis (SAP) remains a serious disease state difficult to manage. Laparoscopic surgery represents a relatively new solution to this problem. This study was aimed to investigate the feasibility of laparoscopic treatment of SAP and the selection of laparoscopic procedures in various stages...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-200310000-00019

    authors: Zhou ZG,Zheng YC,Shu Y,Hu WM,Tian BL,Li QS,Zhang ZD

    更新日期:2003-10-01 00:00:00

  • Immunohistochemical and molecular analysis of giant cell carcinoma of the pancreas: a report of three cases.

    abstract::We performed molecular biological studies as well as immunohistochemical analysis of three cases of giant cell carcinoma of the pancreas. Histologically, one case was a pleomorphic giant cell carcinoma consisting of pleomorphic giant/ small cells and spindle cells, one an osteoclast-like giant cell tumor composed of o...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-199904000-00013

    authors: Imai Y,Morishita S,Ikeda Y,Toyoda M,Ashizawa T,Yamamoto K,Inoue T,Ishikawa T

    更新日期:1999-04-01 00:00:00

  • External validation of 2 prognostic indices for patients with advanced pancreatic cancer treated with first-line therapy.

    abstract:OBJECTIVES:The objective of this study was to perform an external validation of 2 Asian prognostic indices for patients with advanced pancreatic cancer. METHODS:A score was calculated in patients treated with frontline therapy derived from the factors Eastern Cooperative Oncology Group Performance Status, localization...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0b013e31823bcc7b

    authors: Haas M,Laubender RP,Klose C,Schulz C,Mansmann U,Boeck S,Heinemann V

    更新日期:2012-07-01 00:00:00

  • Hypertriglyceridemia Induced Pancreatitis: Inpatient Management at a Single Pediatric Institution.

    abstract:OBJECTIVES:Hypertriglyceridemia-induced pancreatitis is an important cause of acute pancreatitis (AP) in children, which lacks established guidelines. The aim of this study was to review management approaches at a single pediatric center. METHODS:This retrospective study included all inpatients younger than 21 years w...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0000000000001505

    authors: Ippisch HM,Alfaro-Cruz L,Fei L,Zou Y,Thompson T,Abu-El-Haija M

    更新日期:2020-03-01 00:00:00

  • Synthesis of steroids in pancreas: evidence of cytochrome P-450scc activity.

    abstract::In pancreas, the activities of several sex steroid-transforming enzymes have been reported. Data have been obtained in perfused organs, total tissue homogenates, and subcellular organelles. These data, concurrent with the description of the presence of ligand-regulated steroid receptors, as well as the sexually dimorp...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-199907000-00006

    authors: Morales A,Cuéllar A,Ramírez J,Vilchis F,Díaz-Sánchez V

    更新日期:1999-07-01 00:00:00

  • Nonsurgical treatment of acute necrotizing pancreatitis.

    abstract::Our objective was to analyze conservative management in a large series of acute necrotizing pancreatitis (ANP) patients in a specialized center for pancreatic disease in the Internal Medicine and Emergency Department of a 1,500-bed hospital. One hundred ninety-nine patients with ANP were considered (mean age, 55.4 +/-...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-199603000-00006

    authors: Uomo G,Visconti M,Manes G,Calise F,Laccetti M,Rabitti PG

    更新日期:1996-03-01 00:00:00

  • The National Pancreas Foundation fellows symposium program 2006 to 2009.

    abstract::Clinical and translational research is critical for the development of improvement in care of pancreatic diseases. Major concerns are the lack of dedicated trainees in pancreatic research and the difficulty for the remaining trainees to develop independent research careers to be included into the pancreas research com...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0b013e3181c63422

    authors: Gelrud A,Whitcomb DC

    更新日期:2010-04-01 00:00:00

  • Redistribution of clathrin heavy and light chains in anoxic pancreatic acinar cells.

    abstract::In the present study, antibodies directed against clathrin light (33 kDa-36 kDa) and heavy (180 kDa) chains were used to confirm, by immunocytochemistry, that coated vesicles increase in number in the exocrine cells of pancreatic lobules incubated under anoxic (N2) conditions. The same antibodies were used to check wh...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-198603000-00002

    authors: Merisko EM,Farquhar MG,Palade GE

    更新日期:1986-01-01 00:00:00

  • Protective effect of misoprostol, a synthetic prostaglandin E1 analog, on cerulein-induced acute pancreatitis in rats.

    abstract::This study was performed to assess the effects of misoprostol, a synthetic prostaglandin E1 analog, on cerulein-induced pancreatitis. Per group of 10 each, male Wistar rats received either cerulein (2.5 micrograms/kg/h subcutaneously), cerulein and misoprostol (500 micrograms/kg intraperitoneally at 0 and 4 h), or sal...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-199003000-00008

    authors: Buscail L,Bussenot I,Bouisson M,Sénégas-Balas F,Bertrand C,Balas D,Vaysse N,Ribet A

    更新日期:1990-03-01 00:00:00

  • Is Multifocality an Indicator of Aggressive Behavior in Small Bowel Neuroendocrine Tumors?

    abstract:OBJECTIVES:Many patients with small bowel neuroendocrine tumors (SBNETs) have multifocal tumors (MFTs), but the frequency of MFTs has varied widely across SBNET studies. It is also unclear whether patients with MFTs have more advanced disease or worse clinical course than do those with unifocal SBNETs. We set out to de...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0000000000000911

    authors: Choi AB,Maxwell JE,Keck KJ,Bellizzi AJ,Dillon JS,OʼDorisio TM,Howe JR

    更新日期:2017-10-01 00:00:00

  • Nutritional links to plausible mechanisms underlying pancreatic cancer: a conference report.

    abstract::Adenocarcinoma of the pancreas is one of most catastrophic and least understood of cancers. Evidence from clinical studies indicates that the development of pancreas cancer progresses over many years before symptoms appear. Most people with pancreatic cancer die within six months of diagnosis. The lack of early diseas...

    journal_title:Pancreas

    pub_type:

    doi:10.1097/00006676-200311000-00014

    authors: Hine RJ,Srivastava S,Milner JA,Ross SA

    更新日期:2003-11-01 00:00:00

  • Nicotine ameliorates experimental severe acute pancreatitis via enhancing immunoregulation of CD4+ CD25+ regulatory T cells.

    abstract:OBJECTIVES:Activation of "nicotinic anti-inflammatory pathway" could reduce severity of inflammation and injury induced by acute pancreatitis. However, the role of regulatory T (Treg) cells in this pathway is unclear. METHODS:Severe acute pancreatitis (SAP) was induced in mice through retrograde injection of 50-μL 2% ...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0000000000000294

    authors: Zheng YS,Wu ZS,Zhang LY,Ke L,Li WQ,Li N,Li JS

    更新日期:2015-04-01 00:00:00

  • Diagnostic Performance of 48-Hour Fasting Test and Insulin Surrogates in Patients With Suspected Insulinoma.

    abstract:OBJECTIVES:This study aimed to evaluate the usefulness of the 48-hour fasting test and insulin surrogates followed by a glucagon stimulatory test (GST) for the diagnosis of insulinoma. METHODS:Thirty-five patients with suspected insulinoma who underwent 48-hour fasting test and GST were retrospectively included in our...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0000000000000772

    authors: Ueda K,Kawabe K,Lee L,Tachibana Y,Fujimori N,Igarashi H,Oda Y,Jensen RT,Takayanagi R,Ito T

    更新日期:2017-04-01 00:00:00

  • Inhibitory effect of islet amyloid polypeptide of glucose-induced proinsulin biosynthesis in rat insulinoma cells.

    abstract::Islet amyloid polypeptide (IAPP) has been recently identified as the principal constituent of amyloid deposits in pancreatic islets of patients with type 2 (non-insulin-dependent) diabetes mellitus and causes insulin resistance in some target cells. In addition, glucose-induced insulin secretion is inhibited by IAPP. ...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-199207000-00009

    authors: Chuang LM,Wu HP,Jou TS,Tai TY,Lin BJ

    更新日期:1992-01-01 00:00:00

  • Management of a malignant case of solid pseudopapillary tumor of pancreas: a case report and literature review.

    abstract::Solid pseudopapillary tumor of the pancreas is a rare neoplasm with low malignant potential, which affects predominantly young females. Only approximately 10% to 15% cases of solid pseudopapillary tumors (SPTs) are malignant. We present the case of a 57-year-old woman who developed malignant SPT of the pancreas. Meanw...

    journal_title:Pancreas

    pub_type: 杂志文章,评审

    doi:10.1097/MPA.0b013e31824d52c7

    authors: Ji S,Xu J,Zhang B,Xu Y,Liu C,Long J,Ni Q,Yu X

    更新日期:2012-11-01 00:00:00

  • Effect of chronic amylase inhibition on pancreatic growth and acinar cell secretory function in rats.

    abstract::Wheat amylase inhibitor (WAI) was given to growing rats to determine whether chronic inhibition of intraluminal amylase activity alters pancreatic growth, pancreatic enzyme composition, and secretory responsiveness to cholecystokinin octapeptide (CCK-OP) and carbachol. For 21 days 13 rats were fed amylase inhibitor (A...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-199807000-00006

    authors: Kataoka K,DiMagno EP

    更新日期:1998-07-01 00:00:00