Immuno-diagnosis of bubaline fasciolosis with Fasciola gigantica cathepsin-L and recombinant cathepsin L 1-D proteases.

Abstract:

:Fasciola gigantica cathepsin-L cysteine proteinase and recombinant cathepsin L 1-D were assessed for their potential in the immuno-diagnosis of F. gigantica infection in buffaloes. A diagnostic ELISA, based on these two antigens, was developed to detect antibodies against F. gigantica in water buffaloes. Sensitivity of the ELISA was assessed using sera from buffaloes experimentally or naturally infected with F. gigantica from F. gigantica endemic areas and its specificity by probing the sera of the host from F. gigantica non-endemic area. Our earlier studies under experimental setting showed 100% sensitivity of cathepsin-L ELISA in the diagnosis of fasciolosis in buffaloes, with the earliest detection of infection at 4 weeks post-infection. However, under field situation of natural F. gigantica infection, this sensitivity declined to 97.1% but specificity of the test remained 100%. Cross-reactivity of the antigen was checked with Schistosoma indicum, S. spindale, Paramphistomum epiclitum, Gastrothylax spp., Gigantocotyle explanatum, hydatid and Strongyloides papilossus in the bubaline host, naturally infected with these helminths. F. gigantica cathepsin-L and the recombinant cathepsin L-1D does not cross-react with these helminth parasites in natural mono or mixed infection of the host. The present ELISA contributes a relatively sensitive and reliable tool for the early serodiagnosis of bubaline fasciolosis.

journal_name

Acta Trop

journal_title

Acta tropica

authors

Raina OK,Yadav SC,Sriveny D,Gupta SC

doi

10.1016/j.actatropica.2006.03.004

subject

Has Abstract

pub_date

2006-05-01 00:00:00

pages

145-51

issue

2

eissn

0001-706X

issn

1873-6254

pii

S0001-706X(06)00069-6

journal_volume

98

pub_type

杂志文章
  • Advantages of bioconjugated silica-coated nanoparticles as an innovative diagnosis for human toxoplasmosis.

    abstract::Nanotechnology is a promising arena for generating new applications in Medicine. To successfully functionalised nanoparticles for a given biomedical application, a wide range of chemical, physical and biological factors have to be taken into account. Silica-coated nanoparticles, (SiO2NP) exhibit substantial diagnostic...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2017.09.024

    authors: Aly I,Taher EE,El Nain G,El Sayed H,Mohammed FA,Hamad RS,Bayoumy EM

    更新日期:2018-01-01 00:00:00

  • Study of antimicrobial resistance and physiological biomarkers with special reference to Salmonellosis in diarrheic foals in Punjab, Pakistan.

    abstract::Antimicrobial resistance results in selective colonization in animals. In the present study, 447 diarrheic foals (235 horse foals, 165 donkey foals and 47 mule foal) were selected from Lahore and Sahiwal districts of Punjab, Pakistan. Fresh fecal and blood samples from diarrheic foals were collected for isolation and ...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2017.08.003

    authors: Haq I,Durrani AZ,Khan MS,Mushtaq MH,Ahmad I

    更新日期:2017-12-01 00:00:00

  • Schistosomiasis infection in relation to the ABO blood groups among school children in Zimbabwe.

    abstract::The study aimed to establish if there was any relationship between the blood group of the human host and schistosomiasis prevalence, intensity, incidence and related organ pathology. Urine and stool specimens were collected from the 735 school children attending a rural school in Zimbabwe to determine the Schistosoma ...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/s0001-706x(97)00671-2

    authors: Ndamba J,Gomo E,Nyazema N,Makaza N,Kaondera KC

    更新日期:1997-05-30 00:00:00

  • First detection of Leishmania major in peridomestic Phlebotomus papatasi from Isfahan province, Iran: comparison of nested PCR of nuclear ITS ribosomal DNA and semi-nested PCR of minicircle kinetoplast DNA.

    abstract::Two PCR methods were compared for their sensitivity in detecting cultured Leishmania major, before being used to estimate infection rates in female sandflies (Phlebotomus papatasi) collected from peridomestic animal shelters and the nearby burrows of the gerbil reservoir hosts, Rhombomys opimus, in Isfahan province, c...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2004.09.007

    authors: Parvizi P,Mauricio I,Aransay AM,Miles MA,Ready PD

    更新日期:2005-01-01 00:00:00

  • The effect of parasitization by Leishmania mexicana mexicana on macrophage function in vitro.

    abstract::Macrophages infected with amastigotes of Leishmania mexicana mexicana as compared to normal macrophages show decreased migration both randomly and through a 5 microns pore in response to a known chemotaxin, an increased ability to pinocytose and an increased bactericidal ability. Unless very heavily parasitized their ...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:

    authors: Bray RS,Heikal B,Kaye PM,Bray MA

    更新日期:1983-03-01 00:00:00

  • Trypanosoma cruzi persistence at oral inflammatory foci in chronic chagasic patients.

    abstract::The persistence of Trypanosoma cruzi in seropositive individuals, previously diagnosed as chronic chagasic patients (CCP), was detected for the first time in biopsies taken from gingival inflammatory foci processed by polymerase chain reaction (PCR). Seven out of 31 (22.5%) gum samples from selected unquestionably CCP...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2010.12.010

    authors: Añez N,Crisante G,Caraballo F,Delgado W,Parada H

    更新日期:2011-03-01 00:00:00

  • Ethnic differences in susceptibility to malaria: what have we learned from immuno-epidemiological studies in West Africa?

    abstract::There are many fundamental aspects of the immunobiology of Plasmodium falciparum infections that are not fully understood, therefore limiting our comprehension of how people become immune to malaria and why some ethnic groups living in malaria endemic areas are less susceptible than others. The complexity of parasite-...

    journal_title:Acta tropica

    pub_type: 杂志文章,评审

    doi:10.1016/j.actatropica.2015.03.023

    authors: Arama C,Maiga B,Dolo A,Kouriba B,Traoré B,Crompton PD,Pierce SK,Troye-Blomberg M,Miller LH,Doumbo OK

    更新日期:2015-06-01 00:00:00

  • New Cryptosporidium parvum subtypes of IIa subfamily in dairy calves from Brazil.

    abstract::Bovine cryptosporidiosis is mainly caused by four distinct species: Cryptosporidium parvum, C. bovis, C. ryanae and C. andersoni. The first, C. parvum, is a major concern in livestock causing economic losses, in addition to public health impact because of its zoonotic characteristics. The present study aimed to determ...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2013.11.002

    authors: do Couto MC,Lima Mde F,do Bomfim TC

    更新日期:2014-02-01 00:00:00

  • Comparison of disturbance stridulations in five species of triatominae bugs.

    abstract::It has been observed that Triatoma infestans and Rhodnius prolixus females stridulate to reject copulatory attempts performed by males. In addition, triatomines stridulate when disturbed or handled. In the present study, the temporal structure and frequency spectra of vibrational signals produced by mechanically distu...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/s0001-706x(01)00095-x

    authors: Schilman PE,Lazzari CR,Manrique G

    更新日期:2001-05-25 00:00:00

  • Control of soil-transmitted helminths in sub-Saharan Africa: diagnosis, drug efficacy concerns and challenges.

    abstract::Soil-transmitted helminthiasis (STH) occur throughout the developing world and remain a major public health problem in the poorest communities with enormous consequences on health and development of school-aged and preschool children. The extent of the problem has long been neglected because these diseases rarely kill...

    journal_title:Acta tropica

    pub_type: 杂志文章,评审

    doi:10.1016/j.actatropica.2010.07.001

    authors: Tchuem Tchuenté LA

    更新日期:2011-09-01 00:00:00

  • Chemical composition of Litomosoides carinii microfilarial sheaths.

    abstract::Litomosoides carinii microfilariae were exsheathed by freezing and thawing, and the sheaths were separated by filtration. Samples of pure sheaths thus obtained were hydrolyzed, methanolyzed or oxidized with nitric acid under pressure at 300 degrees C, respectively, and were analyzed for amino acids, sugars, fatty acid...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/0001-706x(92)90080-h

    authors: Bardehle G,Jepp-Libutzki A,Linder D,Moehnle K,Schott HH,Zahner H,Zähringer U,Stirm S

    更新日期:1992-02-01 00:00:00

  • Risk of infection of Wuchereria bancrofti to humans by Culex quinquefasciatus in Pondicherry and its relationship with microfilaria prevalence.

    abstract::The risk of infection (RI) with Wuchereria bancrofti by vector mosquitoes has been conventionally estimated from the biting population of all age classes. This estimate of RI had no correlation with microfilaria (mf) prevalence and intensity of infection in humans. A new method for estimating RI from a resting populat...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/0001-706x(93)90081-l

    authors: Vanamail P,Ramaiah KD,Das PK

    更新日期:1993-12-01 00:00:00

  • Prevalence and risk factors for hepatitis B and C viruses in patients with leprosy.

    abstract::It has been reported a higher seroprevalence of HBV and HCV in leprosy patients than in the general population, but the reasons for these findings are not yet clear. On the other hand, there is evidence that these viruses may influence the onset of leprosy reactional episodes, an important cause of neurological sequel...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2017.04.024

    authors: Costa JEF,Morais VMS,Gonçales JP,Silva DM,Coêlho MRCD

    更新日期:2017-08-01 00:00:00

  • Population genetic structure of Schistosoma mansoni and Schistosoma haematobium from across six sub-Saharan African countries: implications for epidemiology, evolution and control.

    abstract::We conducted the first meta-analysis of ten Schistosoma haematobium (one published and nine unpublished) and eight Schistosoma mansoni (two published and six unpublished) microsatellite datasets collected from individual schistosome-infected school-children across six sub-Saharan Africa countries. High levels of genet...

    journal_title:Acta tropica

    pub_type: 杂志文章,meta分析

    doi:10.1016/j.actatropica.2012.09.014

    authors: Gower CM,Gouvras AN,Lamberton PH,Deol A,Shrivastava J,Mutombo PN,Mbuh JV,Norton AJ,Webster BL,Stothard JR,Garba A,Lamine MS,Kariuki C,Lange CN,Mkoji GM,Kabatereine NB,Gabrielli AF,Rudge JW,Fenwick A,Sacko M,Dembel

    更新日期:2013-11-01 00:00:00

  • Vectorial capacity and genetic diversity of Anopheles annularis (Diptera: Culicidae) mosquitoes in Odisha, India from 2009 to 2011.

    abstract::Anopheles annularis is one of the major vectors of malaria in Odisha, India. The present study was undertaken to determine the vectorial capacity and assess the genetic diversity of An. annularis collected from different endemic regions of Odisha. Mosquitoes were collected from thirteen endemic districts using standar...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2014.05.002

    authors: Das B,Patra AP,Das M,Mahapatra N,Tripathy H,Kar SK,Hazra RK

    更新日期:2014-09-01 00:00:00

  • Comparative efficacy of intramuscular artemether and intravenous quinine in Nigerian children with cerebral malaria.

    abstract::The efficacy of a 5-day treatment with intramuscular artemether (3.2-mg/kg loading dose followed by 1.6 mg/kg daily) was compared to that of the standard 7-day treatment with quinine (20-mg/kg loading dose followed by 10 mg/kg every 8 h) in a randomised clinical trial including 103 children aged 12-60 months with cere...

    journal_title:Acta tropica

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1016/s0001-706x(99)00031-5

    authors: Olumese PE,Björkman A,Gbadegesin RA,Adeyemo AA,Walker O

    更新日期:1999-10-15 00:00:00

  • The Clean India Mission: Public and animal health benefits.

    abstract::The Clean India Mission is a national campaign that aims for complete elimination of open defecation from the country. In India, 564 million people do not have access to toilets and defecate in the open environment. The 'Millennium development goals' have given increased weightage to elimination of open defecation for...

    journal_title:Acta tropica

    pub_type: 杂志文章,评审

    doi:10.1016/j.actatropica.2018.06.024

    authors: Thakur R,Singh BB,Jindal P,Aulakh RS,Gill JPS

    更新日期:2018-10-01 00:00:00

  • Characterization of catalytic and non-catalytic activities of EgGST2-3, a heterodimeric glutathione transferase from Echinococcus granulosus.

    abstract::Glutathione transferases (GSTs) perform several catalytic and non-catalytic roles in the defense against toxicities of electrophile compounds and oxidative stress, and therefore are involved in stress-response and cell detoxification. Previously, we have provided evidence indicating that EgGST2 and EgGST3, two phyloge...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2018.01.007

    authors: Lopez-Gonzalez V,La-Rocca S,Arbildi P,Fernandez V

    更新日期:2018-04-01 00:00:00

  • Knowledge and practice of residents about malaria in southeast Iran (1994).

    abstract::A survey was undertaken between November 1994 and January 1995 to assess the knowledge and practice with respect to malaria of 1946 randomly selected households in endemic urban and rural areas of Hormozgan, Kerman and Sistan and Baluchistan Provinces, southeast Iran. Knowledge of inhabitants was relatively poor in th...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/s0001-706x(96)00609-2

    authors: Zaim M,Naseeri-Nejad D,Azoordegan F,Emadi AM

    更新日期:1997-04-15 00:00:00

  • Geographic variation of Trypanosoma cruzi discrete typing units from Triatoma infestans at different spatial scales.

    abstract::We assessed the diversity and distribution of Trypanosoma cruzi discrete typing units (DTU) in Triatoma infestans populations and its association with local vector-borne transmission levels at various geographic scales. At a local scale, we found high predominance (92.4%) of TcVI over TcV in 68 microscope-positive T. ...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2014.07.014

    authors: Fernández Mdel P,Cecere MC,Lanati LA,Lauricella MA,Schijman AG,Gürtler RE,Cardinal MV

    更新日期:2014-12-01 00:00:00

  • Advances in malaria genomics since MIM Arusha, 2002.

    abstract::The promise of new interventions against malaria and other infectious diseases of global health importance derived from pathogen genomic sequence data may only be realized through the coordinated effort of genomic and post-genomics scientists, vaccine and drug developers along with lab- and field-based investigators. ...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2005.06.010

    authors: Carucci DJ

    更新日期:2005-09-01 00:00:00

  • [Polymorphism of Schistosoma mansoni eggs in the Guadeloupe (French Antilles) focus: presence of S. rodhaini-like parasites?].

    abstract::A comparative study of egg morphology of Schistosoma mansoni carried out between 9 parasite populations from Guadeloupean foci demonstrates the existence of an intraspecific polymorphism. Three types of lateral spined eggs have been described and their relative proportions observed between populations are related to t...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:

    authors: Théron A

    更新日期:1986-12-01 00:00:00

  • Flight dispersal of the Chagas disease vectors Triatoma brasiliensis and Triatoma pseudomaculata in northeastern Brazil.

    abstract::The present paper reports for the first time the capture of wild Triatoma brasiliensis and Triatoma pseudomaculata by means of light traps in Brazil. We tested commercially available lighting devices powered by batteries to attract the bugs to a white piece of cloth in the field. Two main findings showed to be signifi...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2006.12.007

    authors: Carbajal de la Fuente AL,Minoli SA,Lopes CM,Noireau F,Lazzari CR,Lorenzo MG

    更新日期:2007-02-01 00:00:00

  • Spatio-temporal dynamics of mosquitoes in stream pools of a biosphere reserve of Southern Western Ghats, India.

    abstract::The spatial and temporal dynamics of mosquitoes in stream pools were examined in a biosphere reserve of the Southern Western Ghats, India. The immature mosquitoes in stream pools were collected from stream substrates of bedrock pool, boulder cavity and sand puddle. The collected larvae and pupae were reared and identi...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2015.09.024

    authors: Anbalagan S,Arunprasanna V,Kannan M,Dinakaran S,Krishnan M

    更新日期:2015-12-01 00:00:00

  • Mansonella perstans filariasis in Africa.

    abstract::Mansonella perstans is a vector-borne human filarial nematode, transmitted by tiny blood-sucking flies (biting midges). It is widespread in many parts of Sub-Saharan Africa and also occurs in parts of Central and South America. Despite the commonness of this parasite very few studies have been carried out on its epide...

    journal_title:Acta tropica

    pub_type: 杂志文章,评审

    doi:10.1016/j.actatropica.2010.01.014

    authors: Simonsen PE,Onapa AW,Asio SM

    更新日期:2011-09-01 00:00:00

  • Leishmania donovani surface glycoconjugate GP36 is the major immunogen component of the fucose-mannose ligand (FML).

    abstract::Leishmania donovani promastigote glycoconjugate ligands, studied in our laboratory, that interact with the internalization receptors on BALB/c macrophages: the 'fucose mannose ligand' (FML), the 'phosphate mannogalactan ligand' (PMGL), and the 'lipopeptidephosphoglycan' (LPPD), interfered also with interaction between...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/0001-706x(93)90006-w

    authors: Palatnik-de-Sousa CB,Dutra HS,Borojevic R

    更新日期:1993-03-01 00:00:00

  • Knowledge, attitude, and practices (KAP) and risk factors analysis related to cystic echinococcosis among residents in Tibetan communities, Xiahe County, Gansu Province, China.

    abstract::Cystic echinococcosis (CE) is a global parasitic zoonosis caused by Echinococcus granulosus. The disease is highly endemic in western China, especially in Tibetan areas, because of poor economic development and hygiene conditions, limited community knowledge of CE, a large scale of dogs, and home slaughtering of lives...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2015.02.018

    authors: Li D,Gao Q,Liu J,Feng Y,Ning W,Dong Y,Tao L,Li J,Tian X,Gu J,Xin D

    更新日期:2015-07-01 00:00:00

  • Relationships between circulating S-antigens, naturally acquired antibodies to Plasmodium falciparum exoantigens and malaria attack in a mesoendemic area.

    abstract::A survey involving 77 individuals living in two savannah villages near Bobo Dioulasso (Burkina Faso, West Africa), was performed in June 1987 (before), August-September (during) and January 1988 after the seasonal transmission. The clinical longitudinal study during the seasonal period permitted us to define three dif...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/0001-706x(92)90064-5

    authors: Chumpitazi BF,Peyron F,Boudin C,Picot S,Oury B,Ambroise-Thomas P

    更新日期:1992-04-01 00:00:00

  • Fluke egg characteristics for the diagnosis of human and animal fascioliasis by Fasciola hepatica and F. gigantica.

    abstract::In trematodiases, shape and size of the fluke eggs shed with faeces are crucial diagnostic features because of their typically reduced intraspecific variability. In fascioliasis, the usual diagnosis during the biliary stage of infection is based on the classification of eggs found in stools, duodenal contents or bile....

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2009.04.005

    authors: Valero MA,Perez-Crespo I,Periago MV,Khoubbane M,Mas-Coma S

    更新日期:2009-08-01 00:00:00

  • Bioinformatics analysis of four proteins of Leishmania donovani to guide epitopes vaccine design and drug targets selection.

    abstract::Visceral leishmaniasis (VL) is a serious and widespread parasitic disease caused by Leishmania donovani complex. The threat of this fatal disease continues due to the lack of ideal drugs or vaccines. In this study, we selected Amastin, CaNA2, Kmp-11 and PDI proteins of Leishmania donovani for study, which are VL vacci...

    journal_title:Acta tropica

    pub_type: 杂志文章

    doi:10.1016/j.actatropica.2018.12.035

    authors: He J,Huang F,Li J,Chen Q,Chen D,Chen J

    更新日期:2019-03-01 00:00:00