A novel protein factor is required for use of distal alternative 5' splice sites in vitro.


:Adenovirus E1A pre-mRNA was used as a model to examine alternative 5' splice site selection during in vitro splicing reactions. Strong preference for the downstream 13S 5' splice site over the upstream 12S or 9S 5' splice sites was observed. However, the 12S 5' splice site was used efficiently when a mutant pre-mRNA lacking the 13S 5' splice site was processed, and 12S splicing from this substrate was not reduced by 13S splicing from a separate pre-mRNA, demonstrating that 13S splicing reduced 12S 5' splice site selection through a bona fide cis-competition. DEAE-cellulose chromatography of nuclear extract yielded two fractions with different splicing activities. The bound fraction contained all components required for efficient splicing of simple substrates but was unable to utilize alternative 5' splice sites. In contrast, the flow-through fraction, which by itself was inactive, contained an activity required for alternative splicing and was shown to stimulate 12S and 9S splicing, while reducing 13S splicing, when added to reactions carried out by the bound fraction. Furthermore, the activity, which we have called distal splicing factor (DSF), enhanced utilization of an upstream 5' splice site on a simian virus 40 early pre-mRNA, suggesting that the factor acts in a position-dependent, substrate-independent fashion. Several lines of evidence are presented suggesting that DSF is a non-small nuclear ribonucleoprotein protein. Finally, we describe a functional interaction between DSF and ASF, a protein that enhances use of downstream 5' splice sites.


Mol Cell Biol


Harper JE,Manley JL




Has Abstract


1991-12-01 00:00:00












  • Developmental characterization and chromosomal mapping of the 5-azacytidine-sensitive fluF locus of Aspergillus nidulans.

    abstract::In Aspergillus nidulans, a fungus that possesses negligible, if any, levels of methylation in its genome, low concentrations of 5-azacytidine (5-AC) convert a high percentage of the cell population to fluffy phenotypic variants through a heritable modification of a single nuclear gene (M. Tamame, F. Antequera, J. R. V...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Tamame M,Antequera F,Santos E

    更新日期:1988-08-01 00:00:00

  • Carcinoembryonic antigen-related cell adhesion molecule 10 expressed specifically early in pregnancy in the decidua is dispensable for normal murine development.

    abstract::The carcinoembryonic antigen (CEA) family consists of a large group of evolutionarily and structurally divergent glycoproteins. The murine CEACAM9 and CEACAM11-related proteins as well as the pregnancy-specific glycoproteins (PSG) are secreted members of the CEA family which are differentially expressed in fetal troph...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Finkenzeller D,Fischer B,Lutz S,Schrewe H,Shimizu T,Zimmermann W

    更新日期:2003-01-01 00:00:00

  • Tiam1 regulates the Wnt/Dvl/Rac1 signaling pathway and the differentiation of midbrain dopaminergic neurons.

    abstract::Understanding the mechanisms that drive the differentiation of dopaminergic (DA) neurons is crucial for successful development of novel therapies for Parkinson's disease, in which DA neurons progressively degenerate. However, the mechanisms underlying the differentiation-promoting effects of Wnt5a on DA precursors are...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Čajánek L,Ganji RS,Henriques-Oliveira C,Theofilopoulos S,Koník P,Bryja V,Arenas E

    更新日期:2013-01-01 00:00:00

  • Inhibition of IkappaB kinase by a new class of retinoid-related anticancer agents that induce apoptosis.

    abstract::The transcription factor NF-kappaB is overexpressed or constitutively activated in many cancer cells, where it induces expression of antiapoptotic genes correlating with resistance to anticancer therapies. Small molecules that inhibit the NF-kappaB signaling pathway could therefore be used to induce apoptosis in NF-ka...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Bayon Y,Ortiz MA,Lopez-Hernandez FJ,Gao F,Karin M,Pfahl M,Piedrafita FJ

    更新日期:2003-02-01 00:00:00

  • Unraveling the intricate organization of mammalian mitochondrial presequence translocases: existence of multiple translocases for maintenance of mitochondrial function.

    abstract::Mitochondria are indispensable organelles implicated in multiple aspects of cellular processes, including tumorigenesis. Heat shock proteins play a critical regulatory role in accurately delivering the nucleus-encoded proteins through membrane-bound presequence translocase (Tim23 complex) machinery. Although altered e...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Sinha D,Srivastava S,Krishna L,D'Silva P

    更新日期:2014-05-01 00:00:00

  • Densely methylated DNA islands in mammalian chromosomal replication origins.

    abstract::Densely methylated DNA sequence islands, designated DMIs, have been observed in two Chinese hamster cell chromosomal replication origins by using a PCR-based chemical method of detection. One of the origins, oriS14, is located within or adjacent to the coding sequence for ribosomal protein S14 on chromosome 2q, and th...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Tasheva ES,Roufa DJ

    更新日期:1994-09-01 00:00:00

  • Identification and analysis of a Saccharomyces cerevisiae copper homeostasis gene encoding a homeodomain protein.

    abstract::Yeast metallothionein, encoded by the CUP1 gene, and its copper-dependent transcriptional activator ACE1 play a key role in mediating copper resistance in Saccharomyces cerevisiae. Using an ethyl methanesulfonate mutant of a yeast strain in which CUP1 and ACE1 were deleted, we isolated a gene, designated CUP9, which p...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Knight SA,Tamai KT,Kosman DJ,Thiele DJ

    更新日期:1994-12-01 00:00:00

  • The Sgs1 helicase of Saccharomyces cerevisiae inhibits retrotransposition of Ty1 multimeric arrays.

    abstract::Ty1 retrotransposons in the yeast Saccharomyces cerevisiae are maintained in a genetically competent but transpositionally dormant state. When located in the ribosomal DNA (rDNA) locus, Ty1 elements are transcriptionally silenced by the specialized heterochromatin that inhibits rDNA repeat recombination. In addition, ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Bryk M,Banerjee M,Conte D Jr,Curcio MJ

    更新日期:2001-08-01 00:00:00

  • Selection of optimal kappa B/Rel DNA-binding motifs: interaction of both subunits of NF-kappa B with DNA is required for transcriptional activation.

    abstract::Analysis of the p50 and p65 subunits of the NF-kappa B transcription factor complex has revealed that both proteins can interact with related DNA sequences through either homo- or heterodimer formation. In addition, the product of the proto-oncogene c-rel can bind to similar DNA motifs by itself or as a heterodimer wi...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Kunsch C,Ruben SM,Rosen CA

    更新日期:1992-10-01 00:00:00

  • ATR1, a Saccharomyces cerevisiae gene encoding a transmembrane protein required for aminotriazole resistance.

    abstract::In Saccharomyces cerevisiae, 3-amino-1,2,4-triazole (aminotriazole) competitively inhibits the activity of imidazoleglycerolphosphate dehydratase, the product of the HIS3 gene. Wild-type strains are able to grow in the presence of 10 mM aminotriazole because they induce the level of imidazoleglycerolphosphate dehydrat...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Kanazawa S,Driscoll M,Struhl K

    更新日期:1988-02-01 00:00:00

  • Construction of microcell hybrid clones containing specific mouse chromosomes: application to autosomes 8 and 17.

    abstract::Fibroblast cultures prepared from mice homozygous for a Robertsonian translocation (centric fusion) between autosomes 8 and 17 [Rb(8.17)] were used as donors in microcell-mediated chromosome transfer experiments. By using hamster recipient cells deficient in adenine phosphoribosyltransferase (APRT-) and selecting for ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Fournier RE,Frelinger JA

    更新日期:1982-05-01 00:00:00

  • Human SNF5/INI1, a component of the human SWI/SNF chromatin remodeling complex, promotes nucleotide excision repair by influencing ATM recruitment and downstream H2AX phosphorylation.

    abstract::Recent studies have implicated the role of the SWI/SNF ATP-dependent chromatin remodeling complex in nuclear excision repair (NER), but the mechanism of its function has remained elusive. Here, we show that the human SWI/SNF component human SNF5 (hSNF5) interacts with UV damage recognition factor XPC and colocalizes w...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Ray A,Mir SN,Wani G,Zhao Q,Battu A,Zhu Q,Wang QE,Wani AA

    更新日期:2009-12-01 00:00:00

  • Association of MEK1 with p21ras.GMPPNP is dependent on B-Raf.

    abstract::We have previously reported that immobilized p21ras forms a GMPPNP-dependent complex with a MEK activity. Furthermore, the association of the MEK activity was found to be independent of the presence of Raf-1. We have extended those observations to show that MEK1 is the MEK activity previously described to associate wi...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Moodie SA,Paris MJ,Kolch W,Wolfman A

    更新日期:1994-11-01 00:00:00

  • A yeast ARS-binding protein activates transcription synergistically in combination with other weak activating factors.

    abstract::ABFI (ARS-binding protein I) is a yeast protein that binds specific DNA sequences associated with several autonomously replicating sequences (ARSs). ABFI also binds sequences located in promoter regions of some yeast genes, including DED1, an essential gene of unknown function that is transcribed constitutively at a h...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Buchman AR,Kornberg RD

    更新日期:1990-03-01 00:00:00

  • Identification of amino acids essential for DNA binding and dimerization in p67SRF: implications for a novel DNA-binding motif.

    abstract::The serum response factor (p67SRF) binds to a palindromic sequence in the c-fos serum response element (SRE). A second protein, p62TCF binds in conjunction with p67SRF to form a ternary complex, and it is through this complex that growth factor-induced transcriptional activation of c-fos is thought to take place. A 90...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Sharrocks AD,Gille H,Shaw PE

    更新日期:1993-01-01 00:00:00

  • MdmX promotes bipolar mitosis to suppress transformation and tumorigenesis in p53-deficient cells and mice.

    abstract::Mdm2 and MdmX are structurally related p53-binding proteins that function as critical negative regulators of p53 activity in embryonic and adult tissue. The overexpression of Mdm2 or MdmX inhibits p53 tumor suppressor functions in vitro, and the amplification of Mdm2 or MdmX is observed in human cancers retaining wild...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Matijasevic Z,Steinman HA,Hoover K,Jones SN

    更新日期:2008-02-01 00:00:00

  • c-Myb contributes to G2/M cell cycle transition in human hematopoietic cells by direct regulation of cyclin B1 expression.

    abstract::Myb family proteins are ubiquitously expressed transcription factors. In mammalian cells, they play a critical role in regulating the G(1)/S cell cycle transition but their role in regulating other cell cycle checkpoints is incompletely defined. Herein, we report experiments which demonstrate that c-Myb upregulates cy...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Nakata Y,Shetzline S,Sakashita C,Kalota A,Rallapalli R,Rudnick SI,Zhang Y,Emerson SG,Gewirtz AM

    更新日期:2007-03-01 00:00:00

  • Cell cycle-specific expression and nuclear binding of DNA polymerase alpha.

    abstract::The expression and distribution of DNA polymerase alpha was measured by cytometry and confocal laser scanning microscopy. Expression was proportional to DNA content in proliferating cells, while only S-phase cells retained DNA polymerase alpha after detergent extraction. Nuclear DNA polymerase alpha binding may be one...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Stokke T,Erikstein B,Holte H,Funderud S,Steen HB

    更新日期:1991-06-01 00:00:00

  • Epstein-Barr virus suppresses a G(2)/M checkpoint activated by genotoxins.

    abstract::Several Epstein-Barr virus (EBV)-negative Burkitt lymphoma-derived cell lines (for example, BL41 and Ramos) are extremely sensitive to genotoxic drugs despite being functionally null for the tumor suppressor p53. They rapidly undergo apoptosis, largely from G(2)/M of the cell cycle. 5-bromo-2'-deoxyuridine labeling ex...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Wade M,Allday MJ

    更新日期:2000-02-01 00:00:00

  • Regulation of CDC9, the Saccharomyces cerevisiae gene that encodes DNA ligase.

    abstract::We have cloned CDC9, the structural gene for Saccharomyces cerevisiae DNA ligase, and investigated its transcriptional regulation both as a function of cell cycle stage and after UV irradiation. The steady-state level of DNA ligase mRNA increases at least fourfold in late G1, after the completion of start but before S...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Peterson TA,Prakash L,Prakash S,Osley MA,Reed SI

    更新日期:1985-01-01 00:00:00

  • Regulated degradation of ornithine decarboxylase requires interaction with the polyamine-inducible protein antizyme.

    abstract::Intracellular degradation of vertebrate ornithine decarboxylase (ODC) is accelerated by polyamines, the products of the pathway controlled by ODC. Antizyme, a reversible, tightly binding protein inhibitor of ODC activity, is believed to be involved in this process. Mouse and Trypanosoma brucei ODCs are structurally si...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Li X,Coffino P

    更新日期:1992-08-01 00:00:00

  • Expression of human Hemojuvelin (HJV) is tightly regulated by two upstream open reading frames in HJV mRNA that respond to iron overload in hepatic cells.

    abstract::The gene encoding human hemojuvelin (HJV) is one of the genes that, when mutated, can cause juvenile hemochromatosis, an early-onset inherited disorder associated with iron overload. The 5' untranslated region of the human HJV mRNA has two upstream open reading frames (uORFs), with 28 and 19 codons formed by two upstr...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Onofre C,Tomé F,Barbosa C,Silva AL,Romão L

    更新日期:2015-04-01 00:00:00

  • Mutant mouse models reveal the relative roles of E2F1 and E2F3 in vivo.

    abstract::The E2F1, -2, and -3 transcription factors are key downstream targets of the retinoblastoma protein (pRB) tumor suppressor that drive expression of proliferation-associated genes. Here we use mutant mouse strains to investigate E2F3's role in vivo. We show that E2F3 is essential for embryonic viability in the pure 129...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Cloud JE,Rogers C,Reza TL,Ziebold U,Stone JR,Picard MH,Caron AM,Bronson RT,Lees JA

    更新日期:2002-04-01 00:00:00

  • Stabilization of the E3 ubiquitin ligase Nrdp1 by the deubiquitinating enzyme USP8.

    abstract::Nrdp1 is a RING finger-containing E3 ubiquitin ligase that physically interacts with and regulates steady-state cellular levels of the ErbB3 and ErbB4 receptor tyrosine kinases and has been implicated in the degradation of the inhibitor-of-apoptosis protein BRUCE. Here we demonstrate that the Nrdp1 protein undergoes e...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Wu X,Yen L,Irwin L,Sweeney C,Carraway KL 3rd

    更新日期:2004-09-01 00:00:00

  • UV radiation facilitates methotrexate resistance and amplification of the dihydrofolate reductase gene in cultured 3T6 mouse cells.

    abstract::Pretreatment of 3T6 murine cells with the carcinogen UV radiation or N-acetoxy-N-acetylaminofluorene increased the number of methotrexate-resistant colonies. This carcinogen-induced enhancement was seen only at low toxicities. The enhancement was transient and was observed at its maximum when cells were subjected to m...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Tlsty TD,Brown PC,Schimke RT

    更新日期:1984-06-01 00:00:00

  • Molecular cloning of the mouse grb2 gene: differential interaction of the Grb2 adaptor protein with epidermal growth factor and nerve growth factor receptors.

    abstract::We report the isolation and molecular characterization of the mouse grb2 gene. The product of this gene, the Grb2 protein, is highly related to the Caenorhabditis elegans sem-5 gene product and the human GRB2 protein and displays the same SH3-SH2-SH3 structural motifs. In situ hybridization studies revealed that the m...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Suen KL,Bustelo XR,Pawson T,Barbacid M

    更新日期:1993-09-01 00:00:00

  • Retinoic acid receptor-mediated induction of ABCA1 in macrophages.

    abstract::ABCA1, the mutant molecule in Tangier Disease, mediates efflux of cellular cholesterol to apoA-I and is induced by liver X receptor (LXR)/retinoid X receptor (RXR) transcription factors. Retinoic acid receptor (RAR) activators (all-trans-retinoic acid [ATRA] and TTNPB) were found to increase ATP-binding cassette trans...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Costet P,Lalanne F,Gerbod-Giannone MC,Molina JR,Fu X,Lund EG,Gudas LJ,Tall AR

    更新日期:2003-11-01 00:00:00

  • Yap, a novel family of eight bZIP proteins in Saccharomyces cerevisiae with distinct biological functions.

    abstract::Saccharomyces cerevisiae contains eight members of a novel and fungus-specific family of bZIP proteins that is defined by four atypical residues on the DNA-binding surface. Two of these proteins, Yap1 and Yap2, are transcriptional activators involved in pleiotropic drug resistance. Although initially described as AP-1...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Fernandes L,Rodrigues-Pousada C,Struhl K

    更新日期:1997-12-01 00:00:00

  • Induction of yeast histone genes by stimulation of stationary-phase cells.

    abstract::In the cell cycle of the budding yeast Saccharomyces cerevisiae, expression of the histone genes H2A and H2B of the TRT1 and TRT2 loci is regulated by the performance of "start," the step that also regulates the cell cycle. Here we show that histone production is also subject to an additional form of regulation that i...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Drebot MA,Veinot-Drebot LM,Singer RA,Johnston GC

    更新日期:1990-12-01 00:00:00

  • Construction and characterization of Chinese hamster cell EmtA EmtB double mutants.

    abstract::Starting with hybrid cell lines between a Chinese hamster cell EmtA mutant and a Chinese hamster cell EmtB mutant, we have constructed cell lines that are homozygous for mutant alleles at both the emtA locus and the emtB locus, by using a two-step segregation protocol. The EmtA EmtB double mutants are approximately 10...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Chang S,Wasmuth JJ

    更新日期:1983-05-01 00:00:00