Novel fluorescent oligoDNA probe bearing a multi-conjugated nucleoside with a fluorophore and a non-fluorescent intercalator as a quencher.

Abstract:

:A set of 15mer linear oligoDNA probes bearing a modified nucleoside conjugated with a polyamine/fluorescein/anthraquinone reporting moiety were synthesized. In a single-stranded form, the fluorescence generated by the excitation of fluorescein was efficiently quenched, while marked recovery of the fluorescence was observed when the probes formed duplexes with the fully complementary strand.

journal_name

Bioorg Med Chem Lett

authors

Kodama S,Asano S,Moriguchi T,Sawai H,Shinozuka K

doi

10.1016/j.bmcl.2006.02.016

subject

Has Abstract

pub_date

2006-05-15 00:00:00

pages

2685-8

issue

10

eissn

0960-894X

issn

1464-3405

pii

S0960-894X(06)00198-3

journal_volume

16

pub_type

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