Polyubiquitin RNA characteristics and conditional induction in sea urchin embryos.


:A cDNA of the sea urchin Strongylocentrotus purpuratus was identified as encoding polyubiquitin and used to detect a single gene with transcripts containing multiple ubiquitin coding units. Polyubiquitin transcripts exist as a 3.2-kb RNA in polyribosomes and as three higher molecular weight RNAs in purified nuclei. The amount of polyubiquitin RNA is essentially constant at 10(4) -10(5) transcripts per embryo during the egg-to-blastula period and then declines during further development. Heat shock elicits a transient increase in the level of polyubiquitin RNA, while Zn(II) ions induce a sustained accumulation, that is influenced by developmental parameters: One round of Zn(II) induction elicits the accumulation of the nuclear 7.6- and 5.6-kb RNAs, as well as the 3.2-kb polysomal RNA; however, a second round of induction yields only the 5.6- and 3.2-kb RNAs, suggestive of a change in pre-mRNA size or processing. Polyubiquitin RNA is expressed equally in ectodermal and mesoendodermal tissues and is induced in both tissue fractions by treatment of pluteus larvae with Zn(II). However, in isolated and cultured tissue fractions, polyubiquitin RNA is not inducible by Zn(II), in contrast to the full inducibility of metallothionein mRNAs. Polyubiquitin RNA induction thus appears to be conditioned by the integrity of the embryo, as well as by previous exposure to inducer.


Dev Biol


Developmental biology


Nemer M,Rondinelli E,Infante D,Infante AA




Has Abstract


1991-06-01 00:00:00














  • Glycoprotein constituents of the vitelline coat of Phallusia mammillata (Ascidiacea) with fertilization inhibiting activity.

    abstract::Vitelline coats (VCs) of Phallusia mammillata were isolated and purified following homogenization of live eggs in order to investigate the molecular basis of sperm-egg recognition. Clean VCs were partly solubilized by sonication in H2O and the soluble fraction (SFVC), derived from the outer surface of VCs, was used fo...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Litscher E,Honegger TG

    更新日期:1991-12-01 00:00:00

  • The insulator protein Suppressor of Hairy wing is required for proper ring canal development during oogenesis in Drosophila.

    abstract::Chromatin insulators orchestrate gene transcription during embryo development and cell differentiation by stabilizing interactions between distant genomic sites. Mutations in genes encoding insulator proteins are generally lethal, making in vivo functional analyses of insulator proteins difficult. In Drosophila, howev...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Hsu SJ,Plata MP,Ernest B,Asgarifar S,Labrador M

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  • Characterization of chicken syndecan-3 as a heparan sulfate proteoglycan and its expression during embryogenesis.

    abstract::Syndecan-3 is one of four identified members of a family of transmembrane proteoglycans (the syndecans) that possess highly similar cytoplasmic and transmembrane domains and may function as extracellular matrix receptors and/or low affinity receptors for signaling molecules such as FGF. We previously reported the clon...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Gould SE,Upholt WB,Kosher RA

    更新日期:1995-04-01 00:00:00

  • Analysis of transcriptional codes for zebrafish dopaminergic neurons reveals essential functions of Arx and Isl1 in prethalamic dopaminergic neuron development.

    abstract::Distinct groups of dopaminergic neurons develop at defined anatomical sites in the brain to modulate function of a large diversity of local and far-ranging circuits. However, the molecular identity as judged from transcription factor expression has not been determined for all dopaminergic groups. Here, we analyze regi...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Filippi A,Jainok C,Driever W

    更新日期:2012-09-01 00:00:00

  • The embryonic cell lineage of the nematode Caenorhabditis elegans.

    abstract::The embryonic cell lineage of Caenorhabditis elegans has been traced from zygote to newly hatched larva, with the result that the entire cell lineage of this organism is now known. During embryogenesis 671 cells are generated; in the hermaphrodite 113 of these (in the male 111) undergo programmed death and the remaind...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Sulston JE,Schierenberg E,White JG,Thomson JN

    更新日期:1983-11-01 00:00:00

  • Zygotic Wnt activity is required for Brachyury expression in the early Xenopus laevis embryo.

    abstract::The canonical, beta-catenin-dependent Wnt pathway is a crucial player in the early events of Xenopus development. Dorsal axis formation and mesoderm patterning are accepted effects of this pathway, but the regulation of expression of genes involved in mesoderm specification is not. This conclusion is based largely on ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Vonica A,Gumbiner BM

    更新日期:2002-10-01 00:00:00

  • Loss of ascl1a prevents secretory cell differentiation within the zebrafish intestinal epithelium resulting in a loss of distal intestinal motility.

    abstract::The vertebrate intestinal epithelium is renewed continuously from stem cells at the base of the crypt in mammals or base of the fold in fish over the life of the organism. As stem cells divide, newly formed epithelial cells make an initial choice between a secretory or enterocyte fate. This choice has previously been ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Roach G,Heath Wallace R,Cameron A,Emrah Ozel R,Hongay CF,Baral R,Andreescu S,Wallace KN

    更新日期:2013-04-15 00:00:00

  • Elk3 is essential for the progression from progenitor to definitive neural crest cell.

    abstract::Elk3/Net/Sap2 (here referred to as Elk3) is an Ets ternary complex transcriptional repressor known for its involvement in angiogenesis during embryonic development. Although Elk3 is expressed in various tissues, additional roles for the protein outside of vasculature development have yet to be reported. Here, we chara...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Rogers CD,Phillips JL,Bronner ME

    更新日期:2013-02-15 00:00:00

  • Identification of a major polypeptide component of the sea urchin fertilization envelope.

    abstract::A monoclonal antibody (MAb No. 25-16), raised against purified cortical secretory vesicles (CVs) from the eggs of Strongylocentrotus purpuratus, has been used to identify a previously uncharacterized CV-derived polypeptide component of the sea urchin fertilization envelope (FE). MAb No. 25-16, an IgG1, bound to a grou...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Vater CA,Jackson RC

    更新日期:1989-03-01 00:00:00

  • Lineage specific trimethylation of H3 on lysine 4 during C. elegans early embryogenesis.

    abstract::In many organisms early embryogenesis is characterised by a period refractory to transcription. In Caenorhabditis elegans, the one-cell embryo is transcriptionally inactive, but at around eight-cell stage transcription is activated in the somatic lineage. This model suggests that histone tail modifications associated ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Wang S,Fisher K,Poulin GB

    更新日期:2011-07-15 00:00:00

  • Coordinate regulation of the expression of axonal proteins by the axonal microenvironment.

    abstract::The axonal functions that act in the formation of the neuronal network have been shown to occur in close interdependence with the tissue that surrounds the growing axons. However, little is known about the molecular building blocks underlying axonal functions, although more than 400 axonal proteins have been identifie...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Sonderegger P,Lemkin PF,Lipkin LE,Nelson PG

    更新日期:1986-11-01 00:00:00

  • Role of basic FGF and oxygen in control of proliferation, survival, and neuronal differentiation in carotid body chromaffin cells.

    abstract::Crest-derived glomus cells of the carotid body (CB) are O(2)-sensitive chemoreceptors, which resemble sympathoadrenal (SA) chromaffin cells. In this study, we tested whether perinatal rat glomus cells are sensitive to basic fibroblast growth factor (bFGF) in vitro and whether their sensitivity is regulated by oxygen. ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Nurse CA,Vollmer C

    更新日期:1997-04-15 00:00:00

  • Dynamic expression of broad-complex isoforms mediates temporal control of an ecdysteroid target gene at the onset of Drosophila metamorphosis.

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    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Mugat B,Brodu V,Kejzlarova-Lepesant J,Antoniewski C,Bayer CA,Fristrom JW,Lepesant JA

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  • Zebrafish Hoxb1a regulates multiple downstream genes including prickle1b.

    abstract::Despite 30 years of Hox gene study, we have a remarkably limited knowledge of the downstream target genes that Hox transcription factors regulate to confer regional identity. Here, we have used a microarray approach to identify genes that function downstream of a single vertebrate Hox gene, zebrafish hoxb1a. This gene...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Rohrschneider MR,Elsen GE,Prince VE

    更新日期:2007-09-15 00:00:00

  • Plant developmental responses to climate change.

    abstract::Climate change is multi-faceted, and includes changing concentrations of greenhouse gases in the atmosphere, rising temperatures, changes in precipitation patterns, and increasing frequency of extreme weather events. Here, we focus on the effects of rising atmospheric CO2 concentrations, rising temperature, and drough...

    journal_title:Developmental biology

    pub_type: 杂志文章,评审


    authors: Gray SB,Brady SM

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  • Evolutionary developmental transition from median to paired morphology of vertebrate fins: Perspectives from twin-tail goldfish.

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    pub_type: 杂志文章,评审


    authors: Abe G,Ota KG

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  • The Pit-1 gene is regulated by distinct early and late pituitary-specific enhancers.

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    journal_title:Developmental biology

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    authors: DiMattia GE,Rhodes SJ,Krones A,Carrière C,O'Connell S,Kalla K,Arias C,Sawchenko P,Rosenfeld MG

    更新日期:1997-02-01 00:00:00

  • The involvement of Eph-Ephrin signaling in tissue separation and convergence during Xenopus gastrulation movements.

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    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Park EC,Cho GS,Kim GH,Choi SC,Han JK

    更新日期:2011-02-15 00:00:00

  • Sprouty gain of function disrupts lens cellular processes and growth by restricting RTK signaling.

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    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Shin EH,Zhao G,Wang Q,Lovicu FJ

    更新日期:2015-10-15 00:00:00

  • Nerve and muscle development in paralysé mutant mice.

    abstract::Nerve and muscle development was studied in paralysé mutant mice. The mutant phenotype is first recognizable 6-7 days after birth (PN 6-PN 7) as cessation of muscle growth and weakness and incoordination of movement. Mutant animals die between 2 and 3 weeks of age. Muscle fibers from paralysé mutants had a unimodal di...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Blondet B,Duxson MJ,Harris AJ,Melki J,Guénet JL,Pinçon-Raymond M,Rieger F

    更新日期:1989-03-01 00:00:00

  • Wnt5a is a transcriptional target of Gli3 and Trps1 at the onset of chondrocyte hypertrophy.

    abstract::During endochondral ossification, the differentiation of proliferating into hypertrophic chondrocytes is a key step determining the pace of bone formation and the future length of the skeletal elements. A variety of transcription factors are expressed at the onset of hypertrophy coordinating the expression of differen...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Wuelling M,Schneider S,Schröther VA,Waterkamp C,Hoffmann D,Vortkamp A

    更新日期:2020-01-01 00:00:00

  • The trafficking protein Tmed2/p24beta(1) is required for morphogenesis of the mouse embryo and placenta.

    abstract::During vesicular transport between the endoplasmic reticulum and the Golgi, members of the TMED/p24 protein family form hetero-oligomeric complexes that facilitate protein-cargo recognition as well as vesicle budding. In addition, they regulate each other's level of expression. Despite analyses of TMED/p24 protein dis...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Jerome-Majewska LA,Achkar T,Luo L,Lupu F,Lacy E

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  • Signals from the dorsal blastopore lip region during gastrulation bias the ectoderm toward a nonepidermal pathway of differentiation in Xenopus laevis.

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    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Savage R,Phillips CR

    更新日期:1989-05-01 00:00:00

  • Generation and analysis of an improved Foxg1-IRES-Cre driver mouse line.

    abstract::Foxg1 expression is highly restricted to the telencephalon and other head structures in the early embryo. This expression pattern has been exploited to generate conditional knockout mice, based on a widely used Foxg1-Cre knock-in line (Foxg1(tm1(cre)Skm)), in which the Foxg1 coding region was replaced by the Cre gene....

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Kawaguchi D,Sahara S,Zembrzycki A,O'Leary DDM

    更新日期:2016-04-01 00:00:00

  • Analysis of mouse kreisler mutants reveals new roles of hindbrain-derived signals in the establishment of the otic neurogenic domain.

    abstract::The inner ear, the sensory organ responsible for hearing and balance, contains specialized sensory and non-sensory epithelia arranged in a highly complex three-dimensional structure. To achieve this complexity, a tight coordination between morphogenesis and cell fate specification is essential during otic development....

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Vázquez-Echeverría C,Dominguez-Frutos E,Charnay P,Schimmang T,Pujades C

    更新日期:2008-10-01 00:00:00

  • SpGCF1, a sea urchin embryo DNA-binding protein, exists as five nested variants encoded by a single mRNA.

    abstract::Several Strongylocentrotus purpuratus gene cis-regulatory regions contain asymmetric C4 sequences which are core elements of target sites for a specific DNA-protein interaction. Blastula stage nuclear extract contains five proteins which specifically bind to these target sites, resulting in a characteristic pattern of...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Zeller RW,Coffman JA,Harrington MG,Britten RJ,Davidson EH

    更新日期:1995-06-01 00:00:00

  • Genetic patterning in the adult capitate antenna of the beetle Tribolium castaneum.

    abstract::Antenna structure varies widely among insects, in contrast to the well-conserved structure of legs. The adult capitate antenna of the red flour beetle, Tribolium castaneum, is composed of eleven articles, organized into four distinct morphological regions (scape, pedicel, funicle and club). Here, we report the use of ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Angelini DR,Kikuchi M,Jockusch EL

    更新日期:2009-03-01 00:00:00

  • Functional analysis of zebrafish GDNF.

    abstract::We have identified zebrafish orthologues of glial cell line-derived neurotrophic factor (GDNF) and the ligand-binding component of its receptor GFRalpha1. We examined the mRNA expression pattern of these genes in the developing spinal cord primary motor neurons (PMN), kidney, and enteric nervous systems (ENS) and have...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Shepherd IT,Beattie CE,Raible DW

    更新日期:2001-03-15 00:00:00

  • Epigenetic discrimination by mouse metaphase II oocytes mediates asymmetric chromatin remodeling independently of meiotic exit.

    abstract::In mammalian fertilization, paternal chromatin is exhaustively remodeled, yet the maternal contribution to this process is unknown. To address this, we prevented the induction of meiotic exit by spermatozoa and examined sperm chromatin remodeling in metaphase II (mII) oocytes. Methylation of paternal H3-K4 and H3-K9 r...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Yoshida N,Brahmajosyula M,Shoji S,Amanai M,Perry AC

    更新日期:2007-01-15 00:00:00

  • The Caenorhabditis elegans NR4A nuclear receptor is required for spermatheca morphogenesis.

    abstract::The gene nhr-6 encodes the Caenorhabditis elegans ortholog of the NR4A nuclear receptor. We determined the biological functions of NHR-6 through the isolation and characterization of a deletion allele of nhr-6, lg6001. We demonstrate that nhr-6 has an essential role in the development of the C. elegans somatic gonad. ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Gissendanner CR,Kelley K,Nguyen TQ,Hoener MC,Sluder AE,Maina CV

    更新日期:2008-01-15 00:00:00