Abstract:
:Fluid shear stress caused by blood flow is a major determinant of vascular remodeling and arterial tone and can lead to development of atherosclerosis. The endothelial monolayer in vivo acts as a signal transduction interface for hemodynamic forces; these forces determine the shape, cytoskeletal organization, and function of endothelial cells, allowing the vessels to cope with physiological or pathological conditions. The Ras superfamily of GTPases have been revealed to be master regulators of many cellular activities. In particular, the GTPases RhoA, Rac1, and Cdc42 are known to regulate cell shape changes through effects on the cytoskeleton, but their ability to influence polarity, microtubule dynamics, and transcription factor activity is just as significant. Shear stress modulates the activity of small GTPases, which are critical for both cytoskeletal reorganization and changes in gene expression in response to shear stress. The goal of this article is to review what is known about Ras and more so about Rho GTPases in mechanotransduction and the responses of cells to fluid flow. Several distinct signaling pathways can be coordinately activated by flow, and small GTPases are strongly implicated in some of them; thus possible connections will be explored and a unifying hypothesis offered.
journal_name
Circ Resjournal_title
Circulation researchauthors
Tzima Edoi
10.1161/01.RES.0000200162.94463.d7subject
Has Abstractpub_date
2006-02-03 00:00:00pages
176-85issue
2eissn
0009-7330issn
1524-4571pii
98/2/176journal_volume
98pub_type
杂志文章,评审abstract::Interaction of carotid sinus and aortic arch reflex control of total peripheral resistance was studied in eight dogs anesthetized with sodium pentobarbital and placed on constant flow cardiac bypass. Carotid sinus and aortic arch baroreceptor areas were isolated and separately perfused at controlled pressures. Combina...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.55.6.740
更新日期:1984-12-01 00:00:00
abstract:RATIONALE:Loss-of-function studies in cardiac myocytes (CMs) are currently limited by the need for appropriate conditional knockout alleles. The factors that regulate CM maturation are poorly understood. Previous studies on CM maturation have been confounded by heart dysfunction caused by whole organ gene inactivation....
journal_title:Circulation research
pub_type: 杂志文章,评审
doi:10.1161/CIRCRESAHA.116.310283
更新日期:2017-06-09 00:00:00
abstract::Raising the hepatic venous pressure experimentally duplicates the type of hepatic congestion seen in many clinical situations including congestive heart failure. Venous pressure was controlled using a hepatic venous long circuit preparation and was raised by 6 cm blood (4.7 mm Hg) or 10 cm (7.8 mm Hg). Total splanchni...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.41.6.787
更新日期:1977-12-01 00:00:00
abstract::Remodeling of the uterine spiral arteries during pregnancy transforms them from high to low resistance vessels that lack vasoconstrictive properties. This process is essential to meet the demand for increased blood flow imposed by the growing fetus. Loss of endothelial and smooth muscle cells (SMC) is evident in remod...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000261352.81736.37
更新日期:2007-03-30 00:00:00
abstract::Originally identified as moderate biological modifiers, microRNAs have recently emerged as powerful regulators of diverse cellular processes with especially important roles in disease and tissue remodeling. The rapid pace of studies on microRNA regulation and function necessitates the development of suitable technique...
journal_title:Circulation research
pub_type: 杂志文章,评审
doi:10.1161/CIRCRESAHA.110.227496
更新日期:2011-01-21 00:00:00
abstract:RATIONALE:Lymphatic vasculature constitutes a second vascular system essential for immune surveillance and tissue fluid homeostasis. Maturation of the hierarchical vascular structure, with a highly branched network of capillaries and ducts, is crucial for its function. Environmental cues mediate the remodeling process,...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.116.308825
更新日期:2017-04-14 00:00:00
abstract::The accumulation of inositol 1,4,5-trisphosphate (IP3) after hormonal stimulation has a physiological role, possibly by alteration of Ca2+ levels in cardiac myocyte. However, this accumulation has not been studied under pathophysiological conditions. In this report, we examine phosphatidylinositol metabolism during ce...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.72.5.966
更新日期:1993-05-01 00:00:00
abstract::A plasma membrane preparation purified from guinea pig ventricles without the use of high concentrations of detergents or structure-disrupting salts was used to compare the mechanisms of controlling sodium, potassium-activated adenosinetriphosphatase (Na, K-ATPase) and adenylate cyclase activities. The basal ATPase ac...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.36.1.8
更新日期:1975-01-01 00:00:00
abstract::The pathogenesis of atherosclerosis and abdominal aortic aneurysm involves substantial proteolysis of the arterial extracellular matrix. The lysosomal cysteine proteases can exert potent elastolytic and collagenolytic activity. Human atherosclerotic plaques have increased cysteine protease content and decreased levels...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000155964.34150.F7
更新日期:2005-02-18 00:00:00
abstract::Tyrosine kinase cascades may play a role in the hypoxic regulation of hypoxia-inducible factor (HIF)-1. We investigated the role of tyrosine kinase phosphorylation and of the Shc/Ras cascade on hypoxic HIF-1 stabilization. Exposure of human umbilical vein endothelial cells to hypoxia results in HIF protein stabilizati...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.0000024412.24491.ca
更新日期:2002-07-12 00:00:00
abstract::A period of prolonged vasodilation follows flow-restricted exercise of skeletal muscle. We tested the hypothesis that adenosine participates in mediating this vascular response. Vascularly isolated, anterior calf muscles of anesthetized dogs were stimulated to contract at a rate of 4 twitches/sec. Blood flow was held ...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.44.6.759
更新日期:1979-06-01 00:00:00
abstract::This article examines the role of innervation in regulating expression of acetylcholinesterase (AchE), butyrylcholinesterase (BuchE), and the muscarinic acetylcholine receptor (mAchR) in avian heart. Two distinct approaches are taken. The first approach examines the relation between the onsets of parasympathetic and s...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.70.4.633
更新日期:1992-04-01 00:00:00
abstract::Structural remodeling of the ventricular wall is a key determinant of clinical outcome in heart disease. Such remodeling involves the production and destruction of extracellular matrix proteins, cell proliferation and migration, and apoptotic and necrotic cell death. Cardiac fibroblasts are crucially involved in these...
journal_title:Circulation research
pub_type: 杂志文章,评审
doi:10.1161/01.res.0000046452.67724.b8
更新日期:2002-12-13 00:00:00
abstract::We examined the function of the interventricular septum in six open-chest dogs by inserting piezoelectric crystals into the interventricular septum. Continuous ultrasonic recordings showed that the changes in myocardial chord length (MCL) in the septum and free walls of the right and left ventricle were similar during...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.49.1.52
更新日期:1981-07-01 00:00:00
abstract::Cyclic GMP modulates gene expression in vascular smooth muscle cells (SMCs) in part by stimulating cGMP-dependent protein kinase I (PKGI) and the phosphorylation of transcription factors. In some cells, cGMP increases nuclear translocation of PKGI and PKGI-dependent phosphorylation of transcription regulators; however...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.108.176321
更新日期:2008-07-03 00:00:00
abstract::Studies of the stellate ganglion and middle cervical ganglion indicate that sympathetic efferent nerve activity can be modified by peripheral excitatory inputs and that these neural connections may function as pathways for a peripheral reflex at the level of the thoracic sympathetic ganglia. This excitatory synaptic i...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.64.3.554
更新日期:1989-03-01 00:00:00
abstract:RATIONALE:Postconditioning at the time of primary percutaneous coronary intervention (PCI) for ST-segment-elevation myocardial infarction may reduce infarct size and improve myocardial salvage. However, clinical trials have shown inconsistent benefit. OBJECTIVE:We performed the first National Heart, Lung, and Blood In...
journal_title:Circulation research
pub_type: 杂志文章,随机对照试验
doi:10.1161/CIRCRESAHA.118.314060
更新日期:2019-03-01 00:00:00
abstract::In 36 normolipemic pigs randomized to a 4-week feeding with regular pig chow (n = 18, control group) or chow supplemented with cod liver oil (1 ml/kg per day) (n = 18, treated group), treatment with cod liver oil produced a significant decrease in serum cholesterol, low density lipoprotein cholesterol, and triglycerid...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.71.4.769
更新日期:1992-10-01 00:00:00
abstract::The rate of renin secretion from renal juxtaglomerular (JG) cells is the major determinant of the activity of the renin-angiotensin system. However, the mechanisms involved in the excretion and turnover of secretory granules in the JG cells remain obscure. Therefore, in the present study, the whole-cell patch-clamp te...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.84.8.929
更新日期:1999-04-30 00:00:00
abstract::Angiotensin II (Ang II) has powerful modulatory actions on cardiovascular function that are mediated by specific receptors located on neurons within the hypothalamus and brain stem. Incubation of neuronal cocultures of rat hypothalamus and brain stem with Ang II elicits an Ang II type 1 (AT1) receptor-mediated inhibit...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.84.3.352
更新日期:1999-02-19 00:00:00
abstract::The subcellular distribution of cardiac muscarinic receptors was defined in canine ventricular myocardium, and receptors were solubilized from subcellular fractions enriched in muscarinic receptor content. The subcellular location of muscarinic receptors in cardiac tissue was determined by measurement of the distribut...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.52.6.664
更新日期:1983-06-01 00:00:00
abstract::During periodic stimulation of spontaneously beating chick heart cell aggregates, there is often an evolution of coupling patterns between the stimulator and the aggregate action potential. For example, at rapid stimulation frequencies, a rhythm that is initially 1:1 (stimulus frequency:aggregate frequency) can evolve...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.69.4.1022
更新日期:1991-10-01 00:00:00
abstract::Changes in angiotensin-converting enzyme were measured in the lungs of fetal rabbits isolated and perfused in situ at varying ages from 22 days gestation to 7 days of age under controlled conditions of flow, pH, and temperature. Enzyme activity was assessed by infusing bradykinin or angiotensin I in Krebs-Henseleit so...
journal_title:Circulation research
pub_type: 杂志文章,收录出版
doi:10.1161/01.res.43.5.705
更新日期:1978-11-01 00:00:00
abstract::The effect of hypercapnia on coronary vascular resistance (CVR) was studied in seven open-chest dogs. Coronary blood flow was supplied to the cannulated left main coronary artery from the femoral artery by a precision pump. Coronary arterial PCO2 was locally controlled with a small membrane oxygenator in the coronary ...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.39.4.558
更新日期:1976-10-01 00:00:00
abstract::The transcriptional regulation of cardiovascular development requires precise spatiotemporal control of gene expression, and heterozygous mutations of transcription factors have frequently been implicated in human cardiovascular malformations. A novel mechanism involving posttranscriptional regulation by small, noncod...
journal_title:Circulation research
pub_type: 杂志文章,评审
doi:10.1161/CIRCRESAHA.108.192872
更新日期:2009-03-27 00:00:00
abstract::The present study investigated the effect of adrenomedullin, a novel vasorelaxant peptide, on the migration of cultured rat vascular smooth muscle cells (SMCs) by using the Boyden-chamber method. Fetal calf serum (FCS) and platelet-derived growth factor (PDGF)-BB strongly stimulated SMC migration. Adrenomedullin clear...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.77.4.660
更新日期:1995-10-01 00:00:00
abstract::The extracellular pH (pHo) and intracellular pH (pHi) were simultaneously measured with H(+)-sensitive microelectrodes in the rabbit papillary muscle during normal arterial perfusion and no-flow ischemia. The preparation was kept in an artificial gaseous atmosphere (N2 and CO2 during ischemia) without a surrounding fl...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.71.2.460
更新日期:1992-08-01 00:00:00
abstract::Using alpha-chloralose-anesthetized cats, we studied blood pressure and heart rate responses to static contraction and passive stretch of the triceps surae muscle before and after microdialyzing the mu-opioid agonist [D-Ala2]-methionine enkephalinamide (DAME, 200 mumol/L) into the L-7 dorsal horn of the spinal cord. I...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.77.2.326
更新日期:1995-08-01 00:00:00
abstract::Isolated cardiac conduction disease is an autosomal dominant defect that includes various combinations of bundle branch or fascicular blocks. These defects can cause sudden death due to a complete heart block. We used a genome-wide screening approach with polymorphic (CA)n repeat markers to determine the chromosomal p...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.77.4.735
更新日期:1995-10-01 00:00:00
abstract::Hyperhomocysteinemia, a risk factor for cardiovascular disease, is caused by nutritional or genetic disturbances in homocysteine metabolism. A polymorphism in methylenetetrahydrofolate reductase (MTHFR) is the most common genetic cause of mild hyperhomocysteinemia. To examine mechanisms by which an elevation in plasma...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000204825.66410.0b
更新日期:2006-03-03 00:00:00