Abstract：
:The cellular and molecular events underlying the formation and differentiation of mesoderm to derivatives such as blood are critical to our understanding of the development and function of many tissues and organ systems. How different mesodermal populations are set aside to form specific lineages is not well understood. Although previous genetic studies in the mouse embryo have pointed to a critical role for the homeobox gene Mix-like (mMix) in gastrulation, its function in mesoderm development remains unclear. Hematopoietic defects have been identified in differentiating embryonic stem cells in which mMix was genetically inactivated. Here we show that conditional induction of mMix in embryonic stem cell-derived embryoid bodies results in the early activation of mesodermal markers prior to expression of Brachyury/T and acceleration of the mesodermal developmental program. Strikingly, increased numbers of mesodermal, hemangioblastic, and hematopoietic progenitors form in response to premature activation of mMix. Differentiation to primitive (embryonic) and definitive (adult type) blood cells proceeds normally and without an apparent bias in the representation of different hematopoietic cell fates. Therefore, the mouse Mix gene functions early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages.
journal_name
Bloodjournal_title
Bloodauthors
Willey S,Ayuso-Sacido A,Zhang H,Fraser ST,Sahr KE,Adlam MJ,Kyba M,Daley GQ,Keller G,Baron MHdoi
10.1182/blood-2005-10-4120subject
Has Abstractpub_date
2006-04-15 00:00:00pages
3122-30issue
8eissn
0006-4971issn
1528-0020pii
2005-10-4120journal_volume
107pub_type
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