Abstract：
BACKGROUND/AIMS:The administration of a methionine and choline deficient (MCD) diet to mice serves as an animal model of NASH. The multidrug resistant 2 (Mdr2) P-glycoprotein encodes for the canalicular phospholipid transporter, and Mdr2 (+/-) mice secrete 40% less phosphatidylcholine than wild-type mice. We have hypothesized that phosphatidylethanolamine-N-methyl transferase (PEMT) up-regulation is a consequence of MCD diet administration, and is important for the pathogenesis of steatohepatitis in this model. However, the effect of decreased phosphatidylcholine secretion and modulation of PEMT on the development of diet-induced steatohepatitis in Mdr2 (+/-) mice has not been explored. Thus, the purpose of the study is to examine the effects of the MCD diet on Mdr2 (+/-) mice. METHODS:Mdr2 (+/-) and Mdr2 (+/+) mice were treated with an MCD or control diet for up to 30 days, and the severity of steatohepatitis, PEMT activity and hepatic S-adenosylmethionine (SAM), and S-adenosylhomocysteine (SAH) levels were measured. RESULTS:Serum ALT levels, hepatic inflammation, and PEMT activity were significantly lower, and hepatic SAM:SAH ratios were significantly higher in Mdr2 (+/-) mice at 7 and 30 days on the MCD diet. CONCLUSIONS:Mdr2 (+/-) mice have diminished susceptibility to MCD diet-induced NASH, which is associated with a relative decrease in PEMT activity and increased SAM:SAH ratios.
journal_name
J Hepatoljournal_title
Journal of hepatologyauthors
Igolnikov AC,Green RMdoi
10.1016/j.jhep.2005.08.026subject
Has Abstractpub_date
2006-03-01 00:00:00pages
586-92issue
3eissn
0168-8278issn
1600-0641pii
S0168-8278(05)00620-3journal_volume
44pub_type
杂志文章abstract：BACKGROUND/AIMS:The natural history of chronic hepatitis C infection during pregnancy has not been clearly established, and thus our aim was to assess serum alanine aminotransferase levels and serum HCV RNA levels during pregnancy. METHODS:Twenty-six pregnant women with chronic hepatitis C were studied. Serum alanine ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(00)80075-6
更新日期：2000-02-01 00:00:00
abstract：BACKGROUND/AIMS:Lamivudine is an antiviral drug that is used to treat hepatitis B virus (HBV) infection. Long-term therapy does not completely suppress viral replication, and resistant mutants emerge. Resistance is mediated by changes in the tyrosine-methionine-apartate-aspartate (YMDD) motif in the catalytic site of t...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2004.02.009
更新日期：2004-06-01 00:00:00
abstract：BACKGROUND & AIMS:Cholangiocarcinoma (CCA) carries a very poor prognosis due to a typically late clinical presentation and a poor response to current therapeutics. While these tumors commonly express PD-1 and PD-L1, the response to immune checkpoint inhibitors (ICI) is poor. Stimulating antigen-presenting cells includi...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2020.11.037
更新日期：2020-12-01 00:00:00
abstract：:Hepatocytes synthesise the majority of serum proteins. This production occurs in the endoplasmic reticulum (ER) and is adjusted by complex local and systemic regulatory mechanisms. Accordingly, serum levels of hepatocyte-made proteins constitute important biomarkers that reflect both systemic processes and the status ...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/j.jhep.2018.04.018
更新日期：2018-08-01 00:00:00
abstract：BACKGROUND & AIMS:Simeprevir is an oral hepatitis C virus (HCV) NS3/4A protease inhibitor approved for treatment of chronic HCV infection. Baseline NS3 polymorphisms in all patients and emerging mutations in patients who failed to achieve sustained virologic response (SVR) with simeprevir plus peginterferon/ribavirin (...
journal_title：Journal of hepatology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.jhep.2014.11.032
更新日期：2015-05-01 00:00:00
abstract：BACKGROUND/AIMS:Hepatitis C virus (HCV) has been recognized as a major cause of liver disease, but little is known about its diffusion at population level. To estimate the prevalence and incidence of HCV infection and to explore potential risk factors at population level, an epidemiologic study was carried out. METHOD...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(97)80276-0
更新日期：1997-07-01 00:00:00
abstract：BACKGROUND/AIMS:Protein-L-isoaspartyl methyltransferase (PIMT) is a methyltransferase that plays a crucial role in the repair of damaged proteins. In this study, we investigated whether ethanol exposure causes an accumulation of modified proteins bearing atypical isoaspartyl residues that may be related to impaired PIM...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2007.01.026
更新日期：2007-06-01 00:00:00
abstract：BACKGROUND/AIMS:The pathogenesis of post-transplantation bone loss is poorly understood, although glucocorticoid therapy is believed to play an important role. In the present study we have measured plasma parathyroid hormone concentrations in the first few months after orthotopic liver transplantation, in order to exam...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(96)80243-1
更新日期：1996-11-01 00:00:00
abstract：BACKGROUND & AIMS:Prevalence data for hepatitis B and C and an evaluation of a guideline based screening in the primary care setting are not yet available. We therefore implemented a hepatitis B surface antigen (HBsAg) and anti-hepatitis C virus (HCV) screening and developed guideline based screening strategies. METHO...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2015.01.011
更新日期：2015-06-01 00:00:00
abstract：BACKGROUND/AIMS:Little is known about the nucleotide excision repair (NER) pathway in the resistance of human hepatocellular carcinoma (HCC) to chemotherapeutics. We investigated expression of several NER genes in human HCC and matching non-tumor tissue (NT) and in normal liver. METHODS:Expression of CSA, CSB, XPC, hH...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2005.02.020
更新日期：2005-08-01 00:00:00
abstract：BACKGROUND/AIM:Restitution of periportal liver necrosis induced by allyl alcohol involves proliferation and differentiation of putative liver stem cells. The participation of different non-epithelial cell types required to restore the liver cord structure in this process has not been well documented. The aim of the stu...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(99)80043-9
更新日期：1999-09-01 00:00:00
abstract：:Pre-S1 (large S) proteins are components of the envelope of HBV. The presence of pre-S1 proteins is correlated with viral replication. To test sera of patients with HBV infection for the presence of antibodies against pre-S1 proteins (anti-pre-S1), an E. coli extract containing a pre-S fusion protein covering the grea...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(87)80005-3
更新日期：1987-02-01 00:00:00
abstract：BACKGROUND/AIM:The aim of this study was to sequence the precore region of HBV isolated from serum and tumorous and non-tumorous liver tissue from patients with hepatocellular carcinoma to identify mutations that might play a role in malignant transformation. METHODS:HBV DNA was extracted from 62 sera, 14 tumorous and...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(98)80212-2
更新日期：1998-01-01 00:00:00
abstract：BACKGROUND:Individuals who reach the antibody threshold level of 10IU/l against the surface protein of the hepatitis B virus (HBV) after completion of a series of hepatitis B vaccination are considered to be long-term protected against a clinically manifest HBV infection. CASE REPORT:Here we describe an acute hepatiti...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2008.07.040
更新日期：2009-02-01 00:00:00
abstract：:The prolylhydroxylase inhibitor HOE 077 which has proven to be liver-selective in animals was tested in general pharmacological and toxicological studies for unwanted effects and safety. The results of these studies permit clinical development. A single-dose study in healthy volunteers was performed which showed that ...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/0168-8278(91)90009-z
更新日期：1991-01-01 00:00:00
abstract：:Hepatitis B virus (HBV) contributes to hepatocellular carcinoma (HCC) development through direct and indirect mechanisms. HBV DNA integration into the host genome occurs at early steps of clonal tumor expansion and induces both genomic instability and direct insertional mutagenesis of diverse cancer-related genes. Pro...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/j.jhep.2016.02.021
更新日期：2016-04-01 00:00:00
abstract：BACKGROUND/AIMS:Herpes simplex virus (HSV) is known to induce fulminant hepatic failure. The aim of this study is to clarify the molecular basis of liver injury in HSV-induced hepatitis. METHODS:Immunocompetent mice were inoculated with HSV or ultraviolet-inactivated HSV (UV-HSV) intravenously. Extent of liver injury ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(03)00385-4
更新日期：2003-11-01 00:00:00
abstract：:Histological fibrosis stage is one of the most important prognostic factors in compensated and decompensated alcohol-related liver disease (ALD). Morphological assessment of fibrosis is useful for patient stratification, enabling individualised management, and for evaluation of treatment effects in clinical studies. I...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/j.jhep.2018.12.003
更新日期：2019-02-01 00:00:00
abstract：BACKGROUND/AIMS:A simple and reproducible hepatic immunohistochemical staining (IHS) for hepatitis C virus (HCV) is not available. We aimed to validate hepatic IHS with monoclonal antibody (Mab) IG222, directed against the HCV-envelope 2 (E2) protein. METHODS:A three-step indirect immunoperoxidase method was used for ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(02)00389-6
更新日期：2003-02-01 00:00:00
abstract：BACKGROUND & AIMS:Accurate evaluation of renal function in patients with liver cirrhosis is critical for clinical management. However, there are still discrepancies between the measured glomerular filtration rate (mGFR) and creatinine-based estimated GFR (eGFR). In this study, we compared the performance of 2 common eG...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2018.12.030
更新日期：2019-05-01 00:00:00
abstract：BACKGROUND & AIMS:Data from clinical trials suggest a potential role for on-treatment monitoring of serum HBsAg titres during interferon-alpha (pegIFN) therapy in predicting virological responses. However, baseline HBsAg titres during the natural history of chronic hepatitis B (CHB) have not been well-characterized. We...
journal_title：Journal of hepatology
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1016/j.jhep.2010.01.007
更新日期：2010-04-01 00:00:00
abstract：BACKGROUND & AIMS:Non-selective beta blockers (NSBBs) have been shown to have deleterious outcomes in patients with refractory ascites, alcoholic hepatitis and spontaneous bacterial peritonitis leading many physicians to stop the drug in these cases. Acute-on-chronic liver failure (ACLF) is characterized by systemic in...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2015.10.018
更新日期：2016-03-01 00:00:00
abstract：:The clinical relevance of a new antimitochondrial antibody, anti-M9, reacting with an outer membrane-associated antigen on liver mitochondria is described. Sera from 22 anti-M2-negative patients with histologically proven primary biliary cirrhosis (PBC) who had been followed for 5-15 years were tested for anti-M9 in t...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(88)80046-1
更新日期：1988-06-01 00:00:00
abstract：BACKGROUND/AIMS:In hepatitis C infection, the production of inappropriate cytokine levels appears to contribute to viral persistence and to affect the response to antiviral therapy. Additionally, polymorphisms in the cytokine genes may affect the production of the cytokines. In this study, we determined the frequency o...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(01)00243-4
更新日期：2002-02-01 00:00:00
abstract：:This review summarizes recent progress in the knowledge of catecholamines in cirrhosis. Compensated patients have normal plasma concentration of noradrenaline. Highly elevated plasma noradrenaline concentration in decompensated patients indicates that the sympathetic nervous system is enhanced in this condition. This ...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/s0168-8278(85)80068-4
更新日期：1985-01-01 00:00:00
abstract：BACKGROUND/AIMS:Little is known about genuine roles of androgens and their receptor in hepatocellular carcinoma. METHODS:In the present study, two sublines derived from a human hepatocellular carcinoma cell line KYN-1 were used: KYN-1/SM10 with androgen receptor and KYN-1/SM2 without androgen receptor. RESULTS:The bi...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/0168-8278(95)80282-7
更新日期：1995-03-01 00:00:00
abstract：BACKGROUND/AIMS:Patients with cholestatic liver disease have 'low-turnover' osteoporosis. Since we reported that bile-duct ligated (BDL) rats develop bone disease with low bone formation and mass, we examined whether their reduced bone mass results in skeletal fragility, and whether the reduction in osteoprogenitor cel...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2003.11.032
更新日期：2004-03-01 00:00:00
abstract：BACKGROUND/AIMS:To analyze the kinetics of the hepatitis C virus and the patterns of resistance to interferon alpha, we assessed HCV RNA levels early during retreatment with high-dose interferon in patients who did not respond to standard treatment. METHODS:Eleven non-responders to previous therapy with 3-6 MU interfe...
journal_title：Journal of hepatology
pub_type： 临床试验,杂志文章
doi：10.1016/s0168-8278(98)80343-7
更新日期：1998-06-01 00:00:00
abstract：:TGR5 is a G protein-coupled receptor expressed in brown adipose tissue and muscle, where its activation by bile acids triggers an increase in energy expenditure and attenuates diet-induced obesity. Using a combination of pharmacological and genetic gain- and loss-of-function studies invivo, we show here that TGR5 sign...
journal_title：Journal of hepatology
pub_type： 评论,杂志文章
doi：10.1016/j.jhep.2010.02.003
更新日期：2010-06-01 00:00:00
abstract：BACKGROUND & AIMS:CD4(+) CD25(+) Foxp3(+) regulatory T cells (Tregs) have a profound ability to control immune responses. We have previously shown that the liver is a major source of peripherally induced Tregs. Here, we investigate the liver cell types and molecular mechanisms responsible for hepatic Treg induction. M...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2014.04.027
更新日期：2014-09-01 00:00:00
