Abstract:
:Over the last years, RNA interference (RNAi) has become a widely used technique that permits the knock-down, and hence functional analysis, of individual genes in vertebrate cells. However, the high failure rate of the RNA molecules used in RNAi experiments continues to be a problem. In this paper, I describe a set of design criteria, experimental steps and expression vectors that can facilitate the effective knock-down of almost any vertebrate gene product in cultured cells or in experimental animals.Gene Therapy (2006) 13, 503-508. doi:10.1038/sj.gt.3302656; published online 29 September 2005.
journal_name
Gene Therjournal_title
Gene therapyauthors
Cullen BRdoi
10.1038/sj.gt.3302656subject
Has Abstractpub_date
2006-03-01 00:00:00pages
503-8issue
6eissn
0969-7128issn
1476-5462pii
3302656journal_volume
13pub_type
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