Abstract：
:The possibility of inducing immune responses to murine interleukin-4 (IL-4) and IL-13 and generating anti-cytokine monoclonal antibodies (mAbs) was studied in IL-4- and IL-13-knockout mice. The minimal doses of IL-4 or IL-13 that could induce significant anti-cytokine responses with titers of 5000-10,000 were 20 microg per injection with the total doses of 100 microg. The highest titers in a range of 20,480-40,960 were achieved by triple immunization of IL-13-knockout mice with 30 microg of IL-13 per injection. Anti-IL-4 mAbs were generated at an antibody serum titer about 400; however, only 0.5% of primary hybridoma clones were anti-IL-4-positive. Anti-IL-13 cell fusions were successful at titers of 20,480 and 40,960 with 50% of the primary clones positive. Both hybridomas secreted low-affinity IgMkappa mAbs and were IL-6-dependent. These data demonstrate that IL-4- and IL-13-deficient mice may develop high polyclonal immune responses to "syngeneic" murine cytokines but fail to generate high-affinity mAbs at the tested conditions.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Shichkin VP,Spivak NYdoi
10.1016/j.imlet.2005.08.005subject
Has Abstractpub_date
2006-02-15 00:00:00pages
148-57issue
2eissn
0165-2478issn
1879-0542pii
S0165-2478(05)00254-3journal_volume
102pub_type
杂志文章abstract：:As one studies more and more the immune systems of invertebrates their multiplicity and diversity increase. On one hand, the number of different local and systemic innate mechanisms (or cascades of mechanisms) increases with every phylum studied, on the other hand the diversity increases within each system as multiple...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2003.10.012
更新日期：2004-03-29 00:00:00
abstract：:Interleukin-33 is an IL-1 family cytokine which signals via its T1/ST2 receptor, and acts as a key regulator of inflammation. This study aims to measure the expression of soluble ST2 (sST2) and IL-33 in endometriosis. We investigated thirty women with laparoscopic and histopathological confirmed endometriosis and 20 c...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2015.05.002
更新日期：2015-07-01 00:00:00
abstract：:The quasimonoclonal (QM) mouse provides a model to analyze B cell selection because major B cell antigen receptors (BCR) are composed of the knockin V(H)DJ(H) 17.2.25 (V(H)T) encoded H chain and the lambda1 or lambda2 L chain, thereby being specific for (4-hydoxy-3-nitrophenyl)acetyl (NP). We have reported that during...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2006.12.011
更新日期：2007-03-15 00:00:00
abstract：:Aging is associated to dysfunctional changes in the immune system, a process termed immunosenescence. Elderly individuals usually present chronic low level inflammation, likely as the consequence of continued exposure to antigens combined with poor immune function, increases in production of pro-inflammatory cytokines...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2014.06.015
更新日期：2014-11-01 00:00:00
abstract：:The attenuated androgen Danazol can partially reverse the biochemical defect and prevent angioedema in patients with inherited C1-inhibitor (C1-INH) deficiency (hereditary angioedema, HAE). Though its clinical effectiveness is independent from significant increase of C1-INH plasma levels, its mechanism of action remai...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(03)00029-4
更新日期：2003-05-01 00:00:00
abstract：:Dendritic cells (DCs) differentiated in the presence of IL-10 preferentially induce regulatory T-cells and tolerance. Whether the tolerogenic properties displayed by these DCs (Tol-DCs) can be overcome has not been fully explored. Here we show for the first time that Tol-DCs express higher levels of TLR5 mRNA, but not...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2009.06.007
更新日期：2009-08-15 00:00:00
abstract：:The leukocyte common antigen, CD45, is one of the major glycoproteins on cells of hemopoietic origin showing considerable heterogeneity in both structure and expression. Biochemical heterogeneity has been attributed to differences in the primary sequence and glycosylation. In this paper we report an additional basis f...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(94)90198-8
更新日期：1994-04-01 00:00:00
abstract：:The carboxy-terminus of the major merozoite surface protein of Plasmodium has been shown to be the target of protective immunity in a number of non-vivax malaria parasite species. In an effort to develop a protective vaccine for Plasmodium vivax, the most prevalent form of human malaria, we expressed in Saccharomyces ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(96)02570-9
更新日期：1996-07-01 00:00:00
abstract：:Even though IgA is considered to play an important role in immunity, surprisingly little is known about the presence of IgA Fc receptor (Fc alpha R)-expressing effector cells in tissues. Difficulties in obtaining human tissue macrophages, led us to study peritoneal macrophages in a human Fc alpha RI transgenic (Tg) mo...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(99)00034-6
更新日期：1999-05-03 00:00:00
abstract：:Type I Diabetes mellitus (DM1) is the effect of T cell dependent autoimmune destruction of insulin producing beta cells in the pancreas islet. T cells are activated in response to islet dominant autoantigens, the result being the development of DM1. Insulin is one of the islet autoantigens responsible for activation o...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(00)00309-6
更新日期：2001-01-15 00:00:00
abstract：:Lymphoid cell infiltration of periodontal tissues undergoing pathological destruction suggests a functional interdependence between connective tissue proteins such as collagen, fibronectin and elastin and mononuclear cells, mainly T lymphocytes. The aim of the study was to assess T lymphocyte proliferation after stimu...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(98)00060-1
更新日期：1998-10-01 00:00:00
abstract：:It is well known that most physiological functions change with aging, including the immune response. Data concerning the aging of lymphocyte subpopulations are conflicting. The antigen density of peripheral blood lymphocytes has been determined by fluorescently tagged OKT-3, OKT-4, OKT-8, OKT-11 and OKM1 monoclonal an...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(90)90072-x
更新日期：1990-02-01 00:00:00
abstract：:Transforming growth factor-beta (TGF-beta) is usually known as an immunosuppressive cytokine, but we and others have shown stimulatory effects of TGF-beta on activation of Th2 T-lymphocytes. In the present investigation we have studied the effect of TGF-beta1 on phosphorylation of ERK, a MAP-kinase downstream of the R...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(00)00184-x
更新日期：2000-06-01 00:00:00
abstract：:The regulatory effect of Z-100 on the balance of Th1/Th2 cell responses in BALB/c mice bearing Meth-A fibrosarcoma was investigated. In tumor bearing mice, Th1 cytokine production (IL-2, IFN-gamma) are suppressed and Th2 cytokine production (IL-4, IL-10) are increased, as compared with those of normal mice. The admini...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(99)00130-3
更新日期：1999-11-01 00:00:00
abstract：:Mechanistic target for rapamycin (mTOR) is a serine/threonine protein kinase that forms two distinct complexes mTORC1 and mTORC2, integrating mitogen and nutrient signals to regulate cell survival and proliferation; processes which are commonly deregulated in human cancers. mTORC1 and mTORC2 have divergent molecular a...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/j.imlet.2018.01.015
更新日期：2018-04-01 00:00:00
abstract：:Age-related changes of different individuals multicentric and polymorphic. We have approached this problem of age-related changes by examining (1) membrane densities of receptors of various ligands in different organs, and (2) changes in different classes of T cells and of antibody response. Three strategies have been...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/0165-2478(87)90147-7
更新日期：1987-12-01 00:00:00
abstract：:Using micro-electrode technique we studied the effects of interleukin-2 (50 ng/ml) on bio-electric activity of rat atrial myocardium under normal conditions and after gradual stretching of the tissue. It was shown that interleukin-2 caused increasing in the duration of action potential at the levels of 25, 50, and 90%...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2015.06.009
更新日期：2015-09-01 00:00:00
abstract：:Mouse spleen cells were activated with IL2 for 4 days in the presence or absence of paraformaldehyde fixed YAC (PFY) tumor cells (spleen cell: PFY ratio 100:1). Fresh spleen cells had poor expression of Ly49A but the expression was significantly upregulated by IL2 activation. Addition of PFYs resulted in a further boo...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(01)00206-1
更新日期：2001-06-01 00:00:00
abstract：:At least three B cell subsets, B-1a (Ly-1B), B-1b and B-2, are present in the mouse periphery. B-1a and B1-b cells represent a small population in the adult spleen and are abundant in the peritonial and pleural cavities. It has been demonstrated in our laboratory that B-1b cells spontaneously proliferated in stationar...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(02)00266-3
更新日期：2003-03-03 00:00:00
abstract：:Microparticles are recently recognized players of intercellular communication. They are involved in signal transduction, cell activation and apoptosis. Their importance is also suggested in autoimmune diseases such as rheumatoid arthritis and systemic sclerosis. We investigated the role of microparticles in polymyosit...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2009.12.018
更新日期：2010-02-16 00:00:00
abstract：:E1, E3-deleted, replication-deficient recombinant adenoviruses are widely studied as vectors for their capacity to transfer therapeutic genes in vivo. They can infect a wide variety of dividing and quiescent cells from different organs and possess a large packaging capacity. One of the major limitations in the use of ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(97)00049-7
更新日期：1997-06-01 00:00:00
abstract：PURPOSE:Transforming growth factor β (TGF-β) is one of the major immune and inflammation factors responsible for regulating cell proliferation, differentiation, angiogenesis, and immune responses. Deregulated TGF-β activity, especially its influence in peritoneal cytokine cross-talk, has been implicated in pathologies ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2018.10.011
更新日期：2018-09-01 00:00:00
abstract：:Interferon-gamma (IFN-gamma) stimulates the development of monocytic features in human myeloid precursors. Because transcriptional regulation of IFN-gamma and the pituitary hormone prolactin (PRL) has been described to involve common Jak-STAT pathways, we addressed here the question of whether PRL plays a role in mono...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(96)02622-3
更新日期：1996-11-01 00:00:00
abstract：:Experiments with malaria in mice suggest that protective immunity depends not only on antibody but also on activation of macrophages. Activated macrophages may cause intra-erythrocytic death of parasites by releasing reactive oxygen intermediates and/or tumour necrosis factor. Macrophage activation for both types of p...
journal_title：Immunology letters
pub_type： 杂志文章,评审
doi：10.1016/0165-2478(85)90173-7
更新日期：1985-01-01 00:00:00
abstract：:We infected umbilical cord blood lymphocytes (CBL) with EBV in vitro. Analysis of the cell population in 3- and 6-day-old cultures showed a relative increase of B cells and outgrowth of B cells after prolonged culture period. The immunomodulator PSK was added to parallel cultures. In these cultures, B cell growth was ...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/s0165-2478(01)00330-3
更新日期：2002-03-01 00:00:00
abstract：:The antibody response in humans naturally primed to a malaria vaccine candidate antigen (Pf155/RESA) is genetically regulated. Here, the impact of antigen presenting cells (APC) on the control of in vitro T-cell responses induced by Pf155/RESA or synthetic peptides corresponding to its major Pf155/RESA epitopes was st...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(94)00163-4
更新日期：1994-12-01 00:00:00
abstract：:The spontaneous migration in vitro, production of T cell migration inhibitory factor (TIF) and the response to TIF of OKT4+ and OKT8+ human T cell subsets were studied. The OKT4+ lymphocytes migrated far better than the OKT8+ cells although the movement of both subsets was comparably inhibited by TIF. The OKT8+ subset...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(86)90122-7
更新日期：1986-08-01 00:00:00
abstract：:This report describes an immunoradiometric assay for Plasmodium falciparum in infected blood, based on a cross-reacting monoclonal antibody (mAb) raised against P. berghei. In this assay, binding of the mAb to intact P. berghei parasites coated on microtiter plates is inhibited by solubilized P. falciparum infected re...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(85)90046-x
更新日期：1985-01-01 00:00:00
abstract：:Differentiation between a non-infective and an infective Leishmania promastigote population was demonstrated. Promastigotes in the stationary phase (day 5) were found to be highly infective in vitro to BALB/c mouse peritoneal macrophages, compared with those of the logarithmic phase (day 3). The infective promastigote...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/0165-2478(85)90114-2
更新日期：1985-01-01 00:00:00
abstract：:Malaria, a major endemic tropical disease, is caused by the infection of blood cells by Plasmodium protozoa. Most patients control their parasitemia by a not fully understood spleen-dependent mechanism. SDF-1alpha is a chemokine produced by stromal cells such as reticular spleen cells. Nitric oxide (NO) has several im...
journal_title：Immunology letters
pub_type： 杂志文章
doi：10.1016/j.imlet.2003.05.001
更新日期：2003-10-31 00:00:00
