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Irreversibly inhibitory kinetics of 3,5-dihydroxyphenyl decanoate on mushroom (Agaricus bisporus) tyrosinase.

Abstract：

:3,5-Dihydroxyphenyl decanoate (DPD) is found to inhibit the diphenolase activity of tyrosinase from mushroom (Agaricus bisporus). The effects of DPD on the diphenolase activity of mushroom tyrosinase have been studied. The results show that the enzyme activity decreases very slowly with an increase in DPD concentrations at lower concentrations of DPD (between 5 and 60 microM). But at higher concentrations of DPD, DPD can strongly inhibit the diphenolase activity of the enzyme and the inhibition is irreversible. The IC50 value was estimated to be 96.5 microM. The inhibition mechanism of DPD has been investigated and the results show that DPD can bind to the free enzyme molecule and enzyme-substrate complex and lose the enzyme activity completely. The inhibition kinetics has been studied in detail by using the kinetic method of the substrate reaction described by Tsou. The microscopic rate constants of the enzyme inhibited by DPD at higher concentrations have been determined.

journal_name

Bioorg Med Chem

journal_title

Bioorganic & medicinal chemistry

authors

Qiu L,Chen QX,Wang Q,Huang H,Song KK

doi

10.1016/j.bmc.2005.06.034

subject

Has Abstract

pub_date

2005-11-15 00:00:00

pages

6206-11

issue

22

eissn

0968-0896

issn

1464-3391

pii

S0968-0896(05)00568-7

journal_volume

13

pub_type

杂志文章

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