Abstract:
:We find that the degree of impairment of protein function by missense variants is predictable by comparative sequence analysis alone. The applicable range of impairment is not confined to binary predictions that distinguish normal from deleterious variants, but extends continuously from mild to severe effects. The accuracy of predictions is strongly dependent on sequence variation and is highest when diverse orthologs are available. High predictive accuracy is achieved by quantification of the physicochemical characteristics in each position of the protein, based on observed evolutionary variation. The strong relationship between physicochemical characteristics of a missense variant and impairment of protein function extends to human disease. By using four diverse proteins for which sufficient comparative sequence data are available, we show that grades of disease, or likelihood of developing cancer, correlate strongly with physicochemical constraint violation by causative amino acid variants.
journal_name
Genome Resjournal_title
Genome researchauthors
Stone EA,Sidow Adoi
10.1101/gr.3804205subject
Has Abstractpub_date
2005-07-01 00:00:00pages
978-86issue
7eissn
1088-9051issn
1549-5469pii
gr.3804205journal_volume
15pub_type
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