Abstract:
:Arylamine N-acetyltransferase (NAT) genes were targeted for inhibition using short hairpin RNA (shRNA) using two different RNA polymerase III promoters. Constructs were developed for NAT1 and NAT2, the endogenous mouse genes, and for human NAT1. There were fetal and neonatal deaths with these constructs, perhaps due in part to an interferon response as reflected in increases in oligoadenylate synthetase I mRNA levels. Seven out of 8 founders with the U6 promoter generated offspring but only 2 gave positive offspring. Out of 15 founders for H1 promoted constructs, only 4 had positive offspring. When transgenic lines were successfully established, the expression of the targeted genes was variable between animals and was not generally inhibitory.
journal_name
J Appl Genetjournal_title
Journal of applied geneticsauthors
Cao W,Hunter R,Strnatka D,McQueen CA,Erickson RPsubject
Has Abstractpub_date
2005-01-01 00:00:00pages
217-25issue
2eissn
1234-1983issn
2190-3883pii
271journal_volume
46pub_type
杂志文章abstract::Pigs as a source of grafts for xenotransplantation can help to overcome the rapidly growing shortage of human donors. However, in the case of pig-to-human transplantation, the antibody-xenoantigen complexes lead to the complement activation and immediate hyperacute rejection. Methods eliminating hyperacute rejection (...
journal_title:Journal of applied genetics
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journal_title:Journal of applied genetics
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更新日期:2005-01-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章,评审
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更新日期:2002-01-01 00:00:00
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journal_title:Journal of applied genetics
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更新日期:2004-01-01 00:00:00
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journal_title:Journal of applied genetics
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更新日期:2005-01-01 00:00:00
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