当前位置： SCI文献检索 > BIOLOGICAL & PHARMACEUTICAL BULLETIN期刊下所有文献 > Molecular and sugar-binding heterogeneity of C-type lectins from Osmerus (Spirinchus) lanceolatus eggs.

Molecular and sugar-binding heterogeneity of C-type lectins from Osmerus (Spirinchus) lanceolatus eggs.

Abstract：

:Two C-type lectins (OLLafs and OLLafl) were isolated from Osmerus (Spirinchus) lanceolatus eggs using asialofetuin-Sepharose column. OLLafs and OLLafl were eluted with 0.2 M sucrose and 0.2 M lactose from the same column, respectively. OLLafl has been estimated to be a heterodimeric protein composed of H- and L-subunit and involved C-type lectin like domain (CTLD). In this study we revealed that OLLafs was a homodimeric protein composed of L-subunit of OLLafl. Although adding EDTA diminished the hemagglutinating activity of OLLafs, the activity of OLLafl was not influenced. Recombinant lectins (rOLLafl-H and -L) and mutant lectins replaced Cys(123, 131 and 136) with Ala (mOLLafl-L(123, 131 and 136)) were established. The activity of mOLLafl-L(136) was comparable to rOLLafl-L, and rOLLafl-H was 15 times lower than rOLLafl-L. On the other hand, the activity of mOLLafl-L(123) and mOLLafl-L(131) were lower than that of rOLLafl-H. Therefore, Cys(136) may not participate in hemagglutinating activity of rOLLafl-L. In contrast, Cys(123) and Cys(131) may partially contribute this activity. Although hemagglutination inhibition profiles of rOLLafl-L, rOLLafl-H and mOLLafl-L(136) were similar, m-OLLafl-L(131)-induced hemagglutination was not inhibited by any sugars tested even at a concentration of 150 mM. Then, Cys(131) may directly contribute to the sugar-binding capacity of OLLafl. Affinities of mOLLafl-L(123) for these sugars were lower than the others. These results suggest that Cys(136) might contribute to the intermolecular disulfide bond in the rOLLafl-L dimer, and that the intramolecular disulfide bond concerning Cys(131) might important for lectin activity.

journal_name

Biol Pharm Bull

journal_title

Biological & pharmaceutical bulletin

authors

Sugawara S,Hosono M,Ogawa Y,Takayanagi M,Nitta K

doi

10.1248/bpb.28.791

subject

Has Abstract

pub_date

2005-05-01 00:00:00

pages

791-6

issue

5

eissn

0918-6158

issn

1347-5215

pii

JST.JSTAGE/bpb/28.791

journal_volume

28

pub_type

杂志文章

相关文献

BIOLOGICAL & PHARMACEUTICAL BULLETIN文献大全
  • Differential Development of Facial and Hind Paw Allodynia in a Nitroglycerin-Induced Mouse Model of Chronic Migraine: Role of Capsaicin Sensitive Primary Afferents.

    abstract：:Despite the relatively high prevalence of migraine or headache, the pathophysiological mechanisms triggering headache-associated peripheral hypersensitivities, are unknown. Since nitric oxide (NO) is well known as a causative factor in the pathogenesis of migraine or migraine-associated hypersensitivities, a mouse mod...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.b17-00589

    authors： Kim SJ,Yeo JH,Yoon SY,Kwon SG,Lee JH,Beitz AJ,Roh DH

    更新日期：2018-02-01 00:00:00

  • Search for new type of PPARγ agonist-like anti-diabetic compounds from medicinal plants.

    abstract：:Potent ligands of peroxisome proliferator-activated receptor γ (PPARγ) such as thiazolidinediones (pioglitazone, troglitazone, etc.) improve insulin sensitivity by increasing the levels of adiponectin, an important adipocytokine associated with insulin sensitivity in adipose tissue. Several constituents from medicinal...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章,评审

    doi：10.1248/bpb.b14-00037

    authors： Matsuda H,Nakamura S,Yoshikawa M

    更新日期：2014-01-01 00:00:00

  • Immunomodulatory effect of Astragali Radix extract on murine TH1/TH2 cell lineage development.

    abstract：:Astragali Radix (AR), is a popular herbal medicine used to treat allergic diseases in Korea, Japan and China. Our study examined the effect of an AR ethanol extract on both in vitro and in vivo murine CD4 T cells' differentiation into Th1 and Th2 subsets. CD4 T cells from Balb/c mice were activated with anti-CD3/anti-...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.27.1946

    authors： Kang H,Ahn KS,Cho C,Bae HS

    更新日期：2004-12-01 00:00:00

  • The cytotoxic activity of the total alkaloids isolated from different parts of Solanum pseudocapsicum.

    abstract：:The total alkaloid fractions of the methanolic extracts of the leaves, ripe fruits, roots, seeds and stem of Solanum pseudocapsicum were subjected to in-vitro cytotoxicity, short-term toxicity and long-term survival studies. All the five fractions exhibited potent activity. The total alkaloid fraction of leaves was fo...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.27.528

    authors： Vijayan P,Vijayaraj P,Setty PH,Hariharpura RC,Godavarthi A,Badami S,Arumugam DS,Bhojraj S

    更新日期：2004-04-01 00:00:00

  • Inhibition of hepatocyte growth factor induction in human dermal fibroblasts by tryptanthrin.

    abstract：:In addition to regulation of normal cell functions, hepatocyte growth factor (HGF) has also been shown to be involved in malignant cell transformation and in growth, invasion and metastasis in cancer cells. Inhibitors of HGF production have a potential for interfering with malignant cell transformation and progression...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.28.260

    authors： Motoki T,Takami Y,Yagi Y,Tai A,Yamamoto I,Gohda E

    更新日期：2005-02-01 00:00:00

  • Antiallergic, antipyretic, hypoglycemic and hepatoprotective effects of aqueous extract of Coronopus didymus Linn.

    abstract：:The aqueous extract of whole plant of Coronopus didymus LINN (CD) [Family: Bracicacea] was screened for antiallergic, antipyretic and hepatoprotective effects in rats and hypoglycemic activity in mice. The extract showed significant antiallergic, antipyretic, hypoglycemic and hepatoprotective activity at 200 and 400 m...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.28.468

    authors： Mantena SK,Mutalik S,Srinivasa H,Subramanian GS,Prabhakar KR,Reddy KR,Srinivasan KK,Unnikrishnan MK

    更新日期：2005-03-01 00:00:00

  • Effect on free radical processes of some ascorbic acid analogues.

    abstract：:Ascorbic acid, present in plasma from humans at concentrations of 50 to 200 mumol/l, has multiple antioxidant properties. Structural modification of this vitamin by the introduction of lipophilic moieties has allowed to the development of ascorbate esters and ethers active as free radical quenchers. Thus, a new series...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.19.220

    authors： Coudert P,Leal F,Duroux E,Rubat C,Couquelet J

    更新日期：1996-02-01 00:00:00

  • Co-localization of TRPV2 and insulin-like growth factor-I receptor in olfactory neurons in adult and fetal mouse.

    abstract：:TRPV2, a member of the transient receptor potential family, has been isolated as a capsaicin-receptor homolog and is thought to respond to noxious heat. Here we show that TRPV2 mRNA is predominantly expressed in the subpopulation of olfactory sensory neurons (OSNs). We carried out histochemical analyses of TRPV2 and i...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.b14-00413

    authors： Matsui H,Noguchi T,Takakusaki K,Kashiwayanagi M

    更新日期：2014-01-01 00:00:00

  • Histamine-releasing properties of T-3762, a novel fluoroquinolone antimicrobial agent in intravenous use. II. Dermovascular permeability-increasing effect and action on peritoneal mast cells.

    abstract：:To predict the actions of T-3762, a newly developed fluoroquinolone antimicrobial agent, as well as ciprofloxacin (CPFX) and ofloxacin (OFLX), on injection sites when dosed parenterally, their ability to increase cutaneous vascular permeability in dogs and to release histamine from rat peritoneal mast cells was examin...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.21.461

    authors： Furuhata K,Fukuda Y,Soumi K,Arai H,Watanabe Y,Narita H

    更新日期：1998-05-01 00:00:00

  • Berberine protects mesenchymal stem cells against hypoxia-induced apoptosis in vitro.

    abstract：:Bone marrow mesenchymal stem cells (MSCs) have the potential to be used in the cellular therapy of solid organs. However, tissue regeneration is limited by the death of transplanted cells. One of the main mechanisms of stem cell death in transplanted organs is through ischemia. In the present study, we sought to inves...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.32.1335

    authors： Zhang W,Su X,Gao Y,Sun B,Yu Y,Wang X,Zhang F

    更新日期：2009-08-01 00:00:00

  • Hair analysis for drugs of abuse. X. Effect of physicochemical properties of drugs on the incorporation rates into hair.

    abstract：:To determine the mechanism involved, the incorporation rate (ICR) of drugs into hair was compared to melanin affinity, lipophilicity and membrane permeability. The following 20 drugs were tested; amphetamine, methamphetamine, p-hydroxyamphetamine, p-hydroxymethamphetamine, cocaine, benzoylecgonine, ecgonine methyl est...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.18.1223

    authors： Nakahara Y,Takahashi K,Kikura R

    更新日期：1995-09-01 00:00:00

  • Determination of urinary and serum pentosidine and its application to elder patients.

    abstract：:Pentosidine, a fluorescent cross-linking compound, accumulates in extracellular matrix, especially in the collagen, and is formed by the nonenzymatic process of advanced Maillard reaction. We developed a method of determination of pentosidine and tried to examine its level in urine and serum of elder patients. The met...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.21.1005

    authors： Yoshihara K,Nakamura K,Kanai M,Nagayama Y,Takahashi S,Saito N,Nagata M

    更新日期：1998-10-01 00:00:00

  • Potentiation of cytotoxic activity in naïve and tumor-bearing mice by oral administration of hot-water extracts from Agaricus brazei fruiting bodies.

    abstract：:When hot-water extracts of mushroom Agaricus brazei MURRILL fruiting bodies (Agaricus extracts) were administered orally into Meth A-bearing mice, tumor growth was significantly inhibited compared with controls given water orally. Treatment with Agaricus extracts for five successive days significantly increased natura...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.27.404

    authors： Takimoto H,Wakita D,Kawaguchi K,Kumazawa Y

    更新日期：2004-03-01 00:00:00

  • Effects of 3,6-dimethylpyrazine-2-thiol on reproductive and accessory reproductive organs in male rats.

    abstract：:The weights of prostate and seminal vesicle were decreased by treatment with 3,6-dimethylpyrazine-2-thiol(3,6-DMP-SH) but the weights of testes and the plasma testosterone levels were not changed. The recovery of the weight of prostate and seminal vesicles of testosterone-treated orchidectomized rats were inhibited by...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.19.360

    authors： Yamada K,Komatsu H,Ohta A

    更新日期：1996-03-01 00:00:00

  • Deoxynybomycin is a selective anti-tumor agent inducing apoptosis and inhibiting topoisomerase I.

    abstract：:Deoxynybomycin was identified as an inducer of p21the/WAF1 gene following screening using a reporter, p21/luciferase. The present study examined its anti-proliferative effect on human tumor cell lines. Deoxynybomycin selectively inhibited growth of human osteoblastic sarcoma Saos-2, gastric cancer TMK-1, and monocytic...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.23.1036

    authors： Egawa K,Yamori T,Nosaka C,Kunimoto S,Takeuchi T,Nos K

    更新日期：2000-09-01 00:00:00

  • Enhanced Oral Bioavailability of Pueraria Flavones by a Novel Solid Self-microemulsifying Drug Delivery System (SMEDDS) Dropping Pills.

    abstract：:To improve bioavailability of pueraria flavones (PF), a self-microemulsifying drug delivery system (SMEDDS) dropping pills composed of PF, Crodamol GTCC, Maisine 35-1, Cremophor RH 40, 1,2-propylene glycol and polyethylene glycol 6000 (PEG6000) was developed. Particle size, zeta potential, morphology and in vitro drug...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.b15-00854

    authors： Guan Q,Zhang G,Sun S,Fan H,Sun C,Zhang S

    更新日期：2016-05-01 00:00:00

  • Silibinin prevents UV-induced HaCaT cell apoptosis partly through inhibition of caspase-8 pathway.

    abstract：:Silymarin is a polyphenolic flavonoid from milk thistle (Silybum marianum), which has anti-inflammatory, cytoprotective, and anticarcinogenic effects. In this study, we assessed the effect of silibinin (Fig. 1), the major active compound in silymarin, on ultraviolet light (UV)-induced cell apoptosis in HaCaT cells, a ...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.29.1096

    authors： Li LH,Wu LJ,Tashiro S,Onodera S,Uchiumi F,Ikejima T

    更新日期：2006-06-01 00:00:00

  • Differential induction of chromosomal aberrations by topoisomerase inhibitors in cultured Chinese hamster cells.

    abstract：:The inducibility of chromosomal aberration was studied using five mammalian topoisomerase inhibitors in Chinese hamster lung fibroblastic cells (CHL) in two protocols involving 6 and 24 h treatment, with respect to the mechanism of chromosomal aberration. In the 6 h treatment plus 18 h recovery culture (6 h treatment)...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.17.222

    authors： Suzuki H,Nakane S

    更新日期：1994-02-01 00:00:00

  • The Anti-hyperlipidemia Effects of Raw Polygonum multiflorum Extract in Vivo.

    abstract：:Polygonum multiflorum is widely used in the prevention and treatment of hyperlipidemia in traditional Chinese Medicine. In this study, the effects and relevant mechanisms of lipid-regulation by raw Polygonum multiflorum (RPM) were investigated. The results indicated that the basal plasma lipids, such as low-density li...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.b17-00218

    authors： Xian Z,Liu Y,Xu W,Duan F,Guo Z,Xiao H

    更新日期：2017-01-01 00:00:00

  • Difference between yeast and bovine mitochondrial ADP/ATP carriers in terms of conformational properties of the first matrix loop as deduced by use of copper-o-phenanthroline.

    abstract：:The mitochondrial ADP/ATP carrier (AAC) has 6 transmembrane regions and 3 matrix loops. Our previous mutational study on the Cys residue in the LM1s of chimeric bovine type 1 AAC (yN-bAAC1), in which the N-terminal 11 amino acids of bovine type 1 AAC are substituted with the corresponding 26 amino acids of yeast type ...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.30.885

    authors： Kihira Y,Ueno M,Terada H

    更新日期：2007-05-01 00:00:00

  • Characterization of the lactose transport system in Citrobacter freundii.

    abstract：:The lactose transport system of Citrobacter freundii was characterized. Both the lactose transport system and beta-galactosidase were induced with either lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG), the latter being the better inducer. The Km values for methyl-beta-D-thiogalactopyranoside (TMG) transport ...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.17.794

    authors： Okazaki N,Tsuda M,Wilson TH,Tsuchiya T

    更新日期：1994-06-01 00:00:00

  • Small-molecule anthracene-induced cytotoxicity and induction of apoptosis through generation of reactive oxygen species.

    abstract：:A series of anthracene derivatives have been synthesized, and their potential individual cytotoxicity was evaluated using Jurkat T cells and peripheral blood mononuclear cells (PBMCs) in vitro. These compounds, except for 2l, showed less cytotoxicity in PBMCs than mitoxantrone. We also analyzed the antiproliferative a...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.27.838

    authors： Chen RF,Chou CL,Wang MR,Chen CF,Liao JF,Ho LK,Tao CW,Huang HS

    更新日期：2004-06-01 00:00:00

  • Extracellular ATP is involved in the induction of apoptosis in murine hematopoietic cells.

    abstract：:Extracellular nucleotides have multiple biological actions in processes such as proliferation, differentiation, chemotaxis, and cytokine secretion through P2X receptors on the cell surface. To determine the biological activity of adenosine triphosphate (ATP) and the expression of P2 nucleotide receptors in murine bone...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.30.671

    authors： Yoon MJ,Lee HJ,Lee YS,Kim JH,Park JK,Chang WK,Shin HC,Kim DK

    更新日期：2007-04-01 00:00:00

  • Investigation of phagocyte-inducible factor in tumor-implanted mice.

    abstract：:The number of spleen Mac-1-positive cells was markedly increased in methylcholanthrene-induced Meth-A fibrosarcoma (Meth-A)-implanted mice. When the sera of Meth-A-implanted mice were added to normal bone marrow cells, granulocyte and macrophage colony stimulating factor (GM-CSF)-dependent growth of bone marrow cells ...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.18.1643

    authors： Watanabe T,Okada A,Mikami T,Matsumoto T,Suzuki M

    更新日期：1995-12-01 00:00:00

  • Antimicrobial resistance and production of biofilms in clinical isolates of coagulase-negative Staphylococcus strains.

    abstract：:Coagulase-negative Staphylococcus (CNS) strains are frequently associated with bacteremia and hospital-acquired infections. 293 CNS strains were isolated from 744 samples from a dialysis center in S. M. de Tucumán, Argentina, from hemocultures, catheters and urine and identified as S. epidermidis, S. haemolyticus, S. ...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.29.1592

    authors： de Allori MC,Jure MA,Romero C,de Castillo ME

    更新日期：2006-08-01 00:00:00

  • Comparative study of skin permeation profiles between brand and generic tulobuterol patches.

    abstract：:Tulobuterol patches are long-acting bronchodilators for percutaneous absorption including the β(2)-adrenoreceptor agonist tulobuterol, as a main ingredient, used for long-term management of pediatric asthma. Since patients who have pediatric asthma often also have atopic dermatitis in which the skin barrier is impaire...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.33.1763

    authors： Yoshihara S,Fukuda H,Abe T,Arisaka O

    更新日期：2010-01-01 00:00:00

  • Is Vulnerability of the Dentate Gyrus to Aging and Amyloid-β1-42 Neurotoxicity Linked with Modified Extracellular Zn2+ Dynamics?

    abstract：:The basal levels of extracellular Zn2+ are in the range of low nanomolar concentrations in the hippocampus and perhaps increase age-dependently. Extracellular Zn2+ dynamics is critical for cognitive activity and excess influx of extracellular Zn2+ into hippocampal neurons is a known cause of cognitive decline. The den...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章,评审

    doi：10.1248/bpb.b17-00871

    authors： Takeda A,Tamano H

    更新日期：2018-01-01 00:00:00

  • Effects of the acetylene compound from Atractylodes rhizome on experimental gastric ulcers induced by active oxygen species.

    abstract：:This study was conducted to determine the beneficial effects of treating digestive disorders of (6E,12E)-tetradecadiene-8,10-diyne-1,3-diol diacetate (TDEYA) detected in the plasma in hydrolyzed form: (6E,12E)-tetradecadiene-8,10-diyne-1,3-diol (TDEY), following the oral administration of a decoction of Atractylodes r...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.17.1364

    authors： Sakurai T,Sugawara H,Saito K,Kano Y

    更新日期：1994-10-01 00:00:00

  • Docetaxel Upregulates HMGB1 Levels in Non-small Cell Lung Cancer.

    abstract：:Immune checkpoint inhibitors (ICIs) exert beneficial effects in non-small cell lung cancer (NSCLC) patients. However, ICIs are only advantageous for a limited population of NSCLC patients. Therefore to enhance their effects, combination therapies with ICIs have been developed. To identify preferable chemotherapy to co...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.b19-00702

    authors： Haruna M,Hirata M,Iwahori K,Kanazawa T,Yamamoto Y,Goto K,Kawashima A,Morimoto-Okazawa A,Funaki S,Shintani Y,Kumanogoh A,Wada H

    更新日期：2020-01-01 00:00:00

  • Multifunctional drug delivery system using starch-alginate beads for controlled release.

    abstract：:Utilizing starch-containing alginate beads, a novel drug delivery system (DDS) was developed. With the starch inside, the composite bead could be dried in its original bead shape and handled in the dried state. By employing alginate multi-coating strategy on the starch-alginate beads, detained or controlled release wa...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.28.394

    authors： Kim YJ,Park HG,Yang YL,Yoon Y,Kim S,Oh E

    更新日期：2005-02-01 00:00:00