当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > In vitro transcription termination activity of the Drosophila mitochondrial DNA-binding protein DmTTF.

In vitro transcription termination activity of the Drosophila mitochondrial DNA-binding protein DmTTF.

Abstract：

:DmTTF is a Drosophila melanogaster mitochondrial DNA-binding protein which binds specifically to two homologous non-coding sequences located at the 3' ends of blocks of genes encoded on opposite strands. In order to test whether this protein acts as transcription termination factor, we assayed the capacity of DmTTF to arrest in vitro the transcription catalyzed by mitochondrial and bacteriophage RNA polymerases. Experiments with human S-100 extracts showed that DmTTF is able to arrest the transcription catalyzed by human mitochondrial RNA polymerase bidirectionally, independently of the orientation of the protein-DNA complex. On the contrary when T3 or T7 RNA polymerases were used, we found that DmTTF prevalently arrests transcription when the DNA-binding site was placed in the reverse orientation with respect to the incoming enzymes. These results demonstrate that DmTTF is a transcription termination factor with a biased polarity and suggest that the DNA-bound protein is structurally asymmetrical, exposing two different faces to RNA polymerases travelling on opposite directions.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Roberti M,Fernandez-Silva P,Polosa PL,Fernandez-Vizarra E,Bruni F,Deceglie S,Montoya J,Gadaleta MN,Cantatore P

doi

10.1016/j.bbrc.2005.03.173

subject

Has Abstract

pub_date

2005-05-27 00:00:00

pages

357-62

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(05)00681-9

journal_volume

331

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Nucleotide sequence of a cDNA clone for human aldolase: a messenger RNA in the liver.

    abstract：:Nearly complete cDNA clones for human aldolase A mRNA were isolated from human liver cDNA library and the nucleotide sequence determined. Using the cDNA clone as a probe the length of human aldolase A mRNAs, isolated from the skeletal muscle, liver and placenta tissues, was measured by RNA blotting and estimated to be...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)91818-2

    authors： Sakakibara M,Mukai T,Hori K

    更新日期：1985-08-30 00:00:00

  • Ethanol infusion increases ANP and p21 gene expression in isolated perfused rat heart.

    abstract：:Whether alcohol-induced heart failure is caused by a direct toxic effect of ethanol, metabolites, or whether it is a secondary result of neurohumoral, hormonal, or nutritional factors is not clear. To address this question a Langendorff retrograde coronary perfusion model of rat heart was used to study the effect of 0...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.4343

    authors： Jänkälä H,Eklund KK,Kokkonen JO,Kovanen PT,Linstedt KA,Härkönen M,Mäki T

    更新日期：2001-02-23 00:00:00

  • Mechanism and significance of specific proteolytic cleavage of Reelin.

    abstract：:Reelin is a secreted glycoprotein essential for normal brain development and function. In the extracellular milieu, Reelin is subject to specific cleavage at two (N-t and C-t) sites. The N-t cleavage of Reelin is implicated in psychiatric and Alzheimer's diseases, but the molecular mechanism and physiological signific...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.01.039

    authors： Kohno S,Kohno T,Nakano Y,Suzuki K,Ishii M,Tagami H,Baba A,Hattori M

    更新日期：2009-02-27 00:00:00

  • Synthesis of nuclear lamins in BHK-21 cells synchronized with aphidicolin.

    abstract：:Lamins A, B and C are the major proteins of mammalian nuclear lamina and have been well studied in BHK-21 cells. By synchronizing BHK-21 cells with aphidicolin, a potent inhibitor of DNA alpha-polymerase, we were able to detect a differential pattern of synthesis for nuclear lamins during the cell cycle. Lamin B start...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80825-8

    authors： Foisy S,Bibor-Hardy V

    更新日期：1988-10-14 00:00:00

  • Bone stroma-derived cells change coregulators recruitment to androgen receptor and decrease cell proliferation in androgen-sensitive and castration-resistant prostate cancer cells.

    abstract：:Prostate cancer (CaP) bone metastasis is an early event that remains inactive until later-stage progression. Reduced levels of circulating androgens, due to andropause or androgen deprivation therapies, alter androgen receptor (AR) coactivator expression. Coactivators shift the balance towards enhanced AR-mediated gen...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.10.009

    authors： Villagran MA,Gutierrez-Castro FA,Pantoja DF,Alarcon JC,Fariña MA,Amigo RF,Muñoz-Godoy NA,Pinilla MG,Peña EA,Gonzalez-Chavarria I,Toledo JR,Rivas CI,Vera JC,McNerney EM,Onate SA

    更新日期：2015-11-27 00:00:00

  • Regulation of hepatic stearoyl-CoA desaturase gene 1 by vitamin A.

    abstract：:The effect of vitamin A supplementation on stearoyl-CoA desaturase gene 1 expression in mouse liver was characterized. Normal BALB/c mice were fed 0.01% and 0.1% retinol palmitate as components of nonpurified diets. This treatment resulted in a 3-fold and a 7-fold induction of SCD1 mRNA levels, respectively, as determ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6070

    authors： Miller CW,Waters KM,Ntambi JM

    更新日期：1997-02-03 00:00:00

  • Analysis of human terminal deoxynucleotidyl transferase cDNA expressible in mammalian cells.

    abstract：:Human Molt3 cDNA library was constructed using pcD vector system which permits the expression of cDNA inserts in mammalian cells. Nearly full-length human terminal deoxynucleotidyltransferase (TdT) cDNA was cloned using a fragment of bovine TdT cDNA as a probe. The human TdT cDNA contains an open reading frame of 1,55...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(87)80493-x

    authors： Koiwai O,Kaneda T,Morishita R

    更新日期：1987-04-14 00:00:00

  • HSF1 and NF-κB p65 participate in the process of exercise preconditioning attenuating pressure overload-induced pathological cardiac hypertrophy.

    abstract：:Pathological cardiac hypertrophy, often accompanied by hypertension, aortic stenosis and valvular defects, is typically associated with myocyte remodeling and cardiac dysfunction. Exercise preconditioning (EP) has been proven to enhance the tolerance of the myocardium to cardiac ischemia-reperfusion injury. However, t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.03.079

    authors： Xu T,Zhang B,Yang F,Cai C,Wang G,Han Q,Zou L

    更新日期：2015-05-08 00:00:00

  • Restrictase free generation of targeting vectors for disruption of complex mouse genes.

    abstract：:Molecular cloning of targeting vectors (TgVs) is a prerequisite procedure for gene disruption in embryonic stem cells. In cases where target genes display complex features (e.g., gene overlap, alternative exon usage), TgVs must mediate deletions with very high precision to prevent unwanted effects. This is often diffi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.04.131

    authors： Miazek A,Cebula A,Skwarek M,Cebrat M,Kisielow P

    更新日期：2007-06-29 00:00:00

  • The identification of Escherichia coli ispB (cel) gene encoding the octaprenyl diphosphate synthase.

    abstract：:The gene located upstream of the nlp gene at min 69 on the chromosome of Escherichia coli was cloned from the plasmid pLC7-42 constructed by Clarke and Carbon. The extract of the strain harboring the plasmid containing this gene showed increased activity of the prenyltransferase. The product of the enzyme reaction was...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1933

    authors： Asai K,Fujisaki S,Nishimura Y,Nishino T,Okada K,Nakagawa T,Kawamukai M,Matsuda H

    更新日期：1994-07-15 00:00:00

  • Gangliosides expressed on breast cancer cells are E-selectin ligands.

    abstract：:Cancer cell adhesion to vascular endothelium is a critical process in hematogenous metastasis. We hypothesized that breast cancer cells express ligands that bind under blood flow conditions to E-selectin expressed by endothelial cells. At a hemodynamic wall shear rate, BT-20 and MDA-MB-468 breast cancer cells adhered ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.02.061

    authors： Shirure VS,Henson KA,Schnaar RL,Nimrichter L,Burdick MM

    更新日期：2011-03-18 00:00:00

  • Antiangiogenic activity of 4-O-methylgallic acid from Canavalia gladiata, a dietary legume.

    abstract：:Development of nontoxic and biologically safe antiangiogenic agent has been highlighted as a promising way to treat angiogenesis related diseases including cancer. Herein, we isolated 4-O-methylgallic acid (4-OMGA) from the seed of Canavalia gladiata, a dietary legume, on the basis of the growth inhibitory activity fo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.03.109

    authors： Jeon KS,Na HJ,Kim YM,Kwon HJ

    更新日期：2005-05-20 00:00:00

  • Inhibition of calcium and calmodulin-dependent phosphodiesterase activity in rats by capsaicin.

    abstract：:Capsaicin, reported to elevate hormone sensitive lipase (HSL), is also found to inhibit the Ca++ and calmodulin-dependent cAMP phosphodiesterase (PDE) activity in adipose tissue of rats, fed high fat diet. The dependence of the enzyme activity on Ca++ and calmodulin in vitro, in control rats, is shown by its substanti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)91109-0

    authors： Salimath BP,Satyanarayana MN

    更新日期：1987-10-14 00:00:00

  • Effects of hyperthyroidism on expression of a phosphoenolpyruvate carboxykinase/bovine growth hormone gene in transgenic mice.

    abstract：:Thyroid hormone (T3) responsiveness of the PEPCK promoter in vivo was examined in both PEPCK/bGH(460) and PEPCK/bGH(335) mouse lines. Transgenic and non-transgenic littermates were treated with methimazole or PTU for 6 or 4 weeks, respectively, then treated +/- T3 for 10 days. In PEPCK/bGH(460) and PEPCK/bGH(355) tran...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0084

    authors： Xu C,McGrane MM

    更新日期：1996-01-17 00:00:00

  • FGF-20, a novel neurotrophic factor, preferentially expressed in the substantia nigra pars compacta of rat brain.

    abstract：:We have isolated cDNA encoding a novel FGF (212 amino acids) from rat brain. Because this is the 20th documented member of the FGF family, we tentatively term it FGF-20. Among FGF family members, FGF-20 is most similar to FGF-9 and FGF-16 (70 and 62% amino acid identity, respectively). Human FGF-20 gene was found in t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3675

    authors： Ohmachi S,Watanabe Y,Mikami T,Kusu N,Ibi T,Akaike A,Itoh N

    更新日期：2000-10-22 00:00:00

  • Do rat hepatic microsomes contain multiple NADPH-supported fatty acid chain elongation pathways or a single pathway?

    abstract：:[2-14C]-trans-2-hexadecenoyl CoA (16:1) and [2-14C]-trans-2-cis-8,11,14-eicosatetraenoyl CoA (20:4) were chemically synthesized and employed as competitive substrates for the liver microsomal trans-2-enoyl CoA reductase component of the fatty acid chain elongation system. Both 7.5 microM and 15 microM 20:4 competitive...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)91059-4

    authors： Nagi MN,Cook L,Prasad MR,Cinti DL

    更新日期：1986-10-15 00:00:00

  • Acyl-trafficking in membrane phospholipid fatty acid turnover: the transfer of fatty acid from the acyl-L-carnitine pool to membrane phospholipids in intact human erythrocytes.

    abstract：:In this work we have investigated the transfer of radioactive palmitic acid between membrane phospholipids and acyl-L-carnitines in intact human erythrocytes. During the incubation period of labeled erythrocyte in non-defatted bovine serum albumin, radioactivity in phosphatidylcholine and phosphatidylethanolamine incr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81500-1

    authors： Arduini A,Tyurin V,Tyuruna Y,Arrigoni-Martelli E,Molajoni F,Dottori S,Federici G

    更新日期：1992-08-31 00:00:00

  • Runx2 function in cells of neural crest origin during intramembranous ossification.

    abstract：:Runt-related transcription factor 2 (Runx2), also known as core binding factor 1 (Cbfa1), is a multifunctional transcription factor and an essential master gene controlling osteoblast differentiation. We previously demonstrated the in vivo functions of Runx2 in mesoderm-derived cells. However, no studies have been con...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.01.059

    authors： Shirai Y,Kawabe K,Tosa I,Tsukamoto S,Yamada D,Takarada T

    更新日期：2019-02-19 00:00:00

  • Solubilization of bacterial cells in organic solvents via reverse micelles.

    abstract：:A reverse micellar system containing Tween 85 and water in isopropylpalmitate was developed which permitted the solubilization of bacteria in the form of homogenous organic solutions. The presence of the bacteria in solution was demonstrated by light microscopy. Immediately after solubilization, isolated bacterial cel...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(85)80030-9

    authors： Häring G,Luisi PL,Meussdoerffer F

    更新日期：1985-03-29 00:00:00

  • A partially folded structure of amyloid-beta(1-40) in an aqueous environment.

    abstract：:Aggregation of the Aβ(1-40) peptide is linked to the development of extracellular plaques characteristic of Alzheimer's disease. While previous studies commonly show the Aβ(1-40) is largely unstructured in solution, we show that Aβ(1-40) can adopt a compact, partially folded structure. In this structure (PDB ID: 2LFM)...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.06.133

    authors： Vivekanandan S,Brender JR,Lee SY,Ramamoorthy A

    更新日期：2011-07-29 00:00:00

  • Molecular markers distinguish bone marrow mesenchymal stem cells from fibroblasts.

    abstract：:To characterize mesenchymal stem cells (MSC), we compared gene expression profiles in human bone marrow MSC (11 lines) and human fibroblasts (4 lines) by RT-PCR and real time PCR. Messenger RNA levels of MHC-DR-alpha, MHC-DR-beta, MHC-DR-associated protein CD74, tissue factor pathway inhibitor-2, and neuroserpin were ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.04.118

    authors： Ishii M,Koike C,Igarashi A,Yamanaka K,Pan H,Higashi Y,Kawaguchi H,Sugiyama M,Kamata N,Iwata T,Matsubara T,Nakamura K,Kurihara H,Tsuji K,Kato Y

    更新日期：2005-06-24 00:00:00

  • Paradoxical effect of L-arginine: acceleration of endothelial cell senescence.

    abstract：:We have recently shown that inhibition of nitric oxide (NO) synthesis by asymmetrical dimethylarginine (ADMA) accelerated endothelial cell (EC) senescence which was prevented by coincubation with L-arginine; however the effect of long-term treatment of l-arginine alone on senescence of ECs have not been investigated. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.06.091

    authors： Scalera F,Closs EI,Flick E,Martens-Lobenhoffer J,Boissel JP,Lendeckel U,Heimburg A,Bode-Böger SM

    更新日期：2009-09-04 00:00:00

  • Molecular characterization of genetic mutation in human lactate dehydrogenase-A (M) deficiency.

    abstract：:Human lactate dehydrogenase-A mutant gene was isolated from the genomic DNA library of a patient deficient in LDH-A (Muscle) subunit. The nucleotide sequences of seven protein-coding exons were determined and a deletion of 20 base-pairs in exon 6 was found. This mutation results in a frame-shift translation and premat...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)92374-9

    authors： Maekawa M,Sudo K,Kanno T,Li SS

    更新日期：1990-04-30 00:00:00

  • Mice lacking DUSP6/8 have enhanced ERK1/2 activity and resistance to diet-induced obesity.

    abstract：:Extracellular signal-regulated kinase 1 and 2 (ERK1/2) have been implicated as important regulators of metabolic homeostasis. Here we generated a new mouse model with genetic deletion of two ERK1/2 phosphatases, dual specificity phosphatase (DUSP) 6 and 8, to further define the role of ERK1/2 in obesity development. D...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.08.106

    authors： Liu R,Peters M,Urban N,Knowlton J,Napierala T,Gabrysiak J

    更新日期：2020-11-26 00:00:00

  • Differential regulation of dynein-driven melanosome movement.

    abstract：:Cytoplasmic dyneins are multisubunit minus-end-directed microtubule motors. Different isoforms of dynein are thought to provide a means for independent movement of different organelles. We investigated the differential regulation of dynein-driven transport of pigment organelles (melanosomes) in Xenopus melanophores. A...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.08.047

    authors： Reilein AR,Serpinskaya AS,Karcher RL,Dujardin DL,Vallee RB,Gelfand VI

    更新日期：2003-09-26 00:00:00

  • SDF-1alpha-induced intracellular calcium transient involves Rho GTPase signalling and is required for migration of hematopoietic progenitor cells.

    abstract：:Signalling through the chemokine stromal derived factor (SDF)-1alpha and its receptor CXCR4 has been recognized as a key event in the migratory response of hematopoietic stem and progenitor cells (HPC). Small GTPases of the Rho/Rac family might be involved in SDF-1alpha signalling at several different levels. In the p...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.10.112

    authors： Henschler R,Piiper A,Bistrian R,Möbest D

    更新日期：2003-11-28 00:00:00

  • Secretion of N-terminal domain of α-dystroglycan in cerebrospinal fluid.

    abstract：:α-Dystroglycan (α-DG) plays crucial roles in maintaining the stability of cells. We demonstrated previously that the N-terminal domain of α-DG (α-DG-N) is secreted by cultured cells into the culture medium. In the present study, to clarify its function in vivo, we generated a monoclonal antibody against α-DG-N and inv...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.06.150

    authors： Saito F,Saito-Arai Y,Nakamura-Okuma A,Ikeda M,Hagiwara H,Masaki T,Shimizu T,Matsumura K

    更新日期：2011-07-29 00:00:00

  • Phospholipase C mimics tumor promoter-induced chemiluminescence in murine epidermal cells.

    abstract：:Treatment of newborn murine epidermal cells with phospholipase C results in the generation of a chemiluminescence response similar to that previously described for phorbol ester tumor promoters. Based on inhibitor studies, the oxidant, believed to be superoxide anion, is most likely generated from lipoxygenase metabol...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)90204-9

    authors： Fischer SM,Baldwin JK,Adams LM

    更新日期：1985-09-30 00:00:00

  • Phosphorylation of the human 1,25-dihydroxyvitamin D3 receptor by cAMP-dependent protein kinase, in vitro, and in transfected COS-7 cells.

    abstract：:We report that the human 1,25-dihydroxyvitamin D3 receptor is an efficient substrate for cAMP-dependent protein kinase, in vitro. This phosphorylation reaction is rapid and neither dependent upon nor significantly affected by the presence of the 1,25-dihydroxyvitamin D3 ligand. Preliminary mapping experiments utilizin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1328

    authors： Jurutka PW,Hsieh JC,Haussler MR

    更新日期：1993-03-31 00:00:00

  • Tyrosine kinase-dependent phosphatidylinostiol turnover and functional responses in the Fc epsilon R1 signalling pathway.

    abstract：:In RBL-2H3 rat basophilic leukemia cells, Fc epsilon R1 crosslinking by multivalent antigen stimulates phosphatidylinositol (PI) turnover and Ca2+ influx and causes functional responses that include secretion, membrane ruffling and actin polymerization. Here, we show that the tyrosine kinase inhibitor, genistein, inhi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91406-3

    authors： Deanin GG,Martinez AM,Pfeiffer JR,Gardner ME,Oliver JM

    更新日期：1991-08-30 00:00:00