Abstract:
:On the Mycobacterium tuberculosis genome there are four mce operons, all of which are similar in sequence and organization, and code for putatively exported proteins. To investigate whether Mce proteins are essential for virulence, we generated knock-out mutants in mce1, mce2 and mce3 operons of M. tuberculosis and evaluated their ability to multiply in a mammalian host. The allelic replacement was confirmed in each mutant strain by Southern blotting. RT-PCR experiments demonstrated the lack of in vitro expression of mutated genes in Deltamce1 and Deltamce2 mutants. On the other hand, no expression of mce3 was detected in either the wild-type or mutant strains. Similar doubling time and growth characteristics in in vitro culture were observed for mutants and parental strains. The intratracheal route was used to infect BALB/c mice with the Deltamce3, Deltamce2 and Deltamce1 mutants. Ten weeks after infection, all mice infected with the Deltamce mutants survived, while those infected with the wild-type strain died. This long survival correlated with very low counts of colony-forming units (CFU) in the lungs. Deltamce1-infected mice developed very few and small granulomas, while animals infected with Deltamce3 or Deltamce2 mutants showed delayed granuloma formation. Mice infected with Deltamce1 did not develop pneumonia, while animals infected with Deltamce3 and Deltamce2 mutants showed small pneumonic patches. In spleens, bacterial counts of mutant strains were less reduced than in lungs, compared with those of wild-type. In contrast, no such attenuation was observed when the intraperitoneal route was used for infection. Moreover, Deltamce1 mutants appear to be more virulent in lungs after intraperitoneal inoculation. In conclusion, mce operons seem to affect the virulence of M. tuberculosis in mice, depending on the route of infection. Hypotheses are discussed to explain this last issue. Thus, mutants in these genes seem to be good candidates for vaccine testing.
journal_name
Microbes Infectjournal_title
Microbes and infectionauthors
Gioffré A,Infante E,Aguilar D,Santangelo MP,Klepp L,Amadio A,Meikle V,Etchechoury I,Romano MI,Cataldi A,Hernández RP,Bigi Fdoi
10.1016/j.micinf.2004.11.007subject
Has Abstractpub_date
2005-03-01 00:00:00pages
325-34issue
3eissn
1286-4579issn
1769-714Xpii
S1286-4579(05)00009-2journal_volume
7pub_type
杂志文章abstract::There is increasing evidence that myeloid cells express several receptor families, which include both inhibitory and stimulatory isoforms. The expression of receptor isoforms with similar specificities but opposite functions on the same cell is intriguing. What might be the interplay between these receptors? Some clue...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/s1286-4579(00)00294-x
更新日期:2000-03-01 00:00:00
abstract::We previously found that, in a mouse model of Chagas cardiomyopathy, 18% of the 9390 quantified unigenes were significantly regulated by Trypanosoma cruzi infection. However, treatment with bone marrow-derived mononuclear cells (MNCs) resulted in 84% transcriptomic recovery. We have applied new algorithms to reanalyze...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2017.11.003
更新日期:2018-03-01 00:00:00
abstract::This study analyzed macrophage inflammatory protein-2 (MIP-2) production and neutrophil recruitment in urinary tract in response to Pseudomonas aeruginosa in an ascending model of urinary tract infection (UTI) in mice. Both planktonic and biofilm cells of P. aeruginosa were used for inducing UTI in mice. MIP-2 levels ...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2004.08.008
更新日期:2004-11-01 00:00:00
abstract::We investigated whether a 6-amino acid insertion/deletion polymorphism in the mucin domain of TIM-1 (T-cell immunoglobulin and mucin domain 1), modulates susceptibility to HIV-1 infection. The polymorphism was genotyped in three case/control cohorts of HIV-1 exposed seronegative individuals (HESN) and HIV-1 infected s...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2016.09.005
更新日期:2017-01-01 00:00:00
abstract::We investigated the thymic and peripheral T-lymphocyte subsets in BALB/c mice undergoing acute or chronic Trypanosoma cruzi infection, in terms of expression of particular Vbeta rearrangements of the T-cell receptor. We first confirmed the severe depletion of CD4(+)CD8(+) thymocytes following acute T. cruzi infection....
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/s1286-4579(03)00156-4
更新日期:2003-08-01 00:00:00
abstract::Drug-resistant tuberculosis (TB), including the more severe forms of multidrug- and extensively drug-resistant forms, is an increasing public health concern globally. In Sweden the majority of patients with TB are immigrants from countries with a high incidence of TB including the drug-resistant forms. In this study, ...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2008.03.006
更新日期:2008-05-01 00:00:00
abstract::Emerging evidence identifies multiple roles for nucleoside-diphosphate-kinase in host-microbe interaction. We provide the first synopsis of utilization of this molecule by various microorganisms during colonization of host tissues. Additionally, we propose novel mechanisms this effector may participate in, which could...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2011.10.002
更新日期:2012-03-01 00:00:00
abstract::Superinfection rates of human immunodeficiency virus type 1 (HIV-1) have increasingly been leading to more variation in HIV-1, as evidenced by the emergence of circulating recombinant forms (CRFs). We recently reported complementation in a persistently replication-defective subtype B-infected cell clone, L-2, by super...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2008.01.012
更新日期:2008-04-01 00:00:00
abstract::The role of virus-derived small RNAs (vsRNAs) has been identified as an antiviral mechanism in plants, arthropods, and nematodes. Although mammalian DNA viruses have been observed to encode functional miRNAs, whether RNA virus infection generates functional vsRNAs remains under discussion. This article reviews the mos...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2015.04.005
更新日期:2015-08-01 00:00:00
abstract::Parasites of the genus Schistosoma are organisms capable of living for decades within the definitive host. They interfere with the immune response by interacting with host's receptors. In this review, we discuss from the first reports to the most recent discoveries regarding the ability of Schistosoma antigens in trig...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2020.08.001
更新日期:2020-01-01 00:00:00
abstract::The primary purpose of preoperative dental screening of medical patients is to detect acute or chronic oral conditions that may require management prior to planned medical interventions. The aim of this communication is to discuss the background of preoperative dental screening and the link between dental pathologies ...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2016.10.002
更新日期:2017-02-01 00:00:00
abstract::Cytolytic T-cell responses from 63 normal blood donors were monitored in a Mycobacterium bovis BCG infection system in vitro. We wanted to know whether cultured dendritic cells were capable of potentiating the cytolytic T-cell responses to M. bovis BCG. Infected cultured dendritic cells were up to ten times more effec...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/s1286-4579(99)80077-x
更新日期:1999-08-01 00:00:00
abstract::Enterovirus 71 (EV71) is a neurotropic pathogen that can cause severe neural diseases and complications on infected patients. Clinical observations showed that EV71-induced immune responses may be associated with virus induced neurogenic pulmonary edema. Here reviewed studies that discovered several host molecules as ...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2010.03.006
更新日期:2010-07-01 00:00:00
abstract::The early interferon-gamma (IFN-gamma) response after Salmonella infection was compared in resistant and susceptible congenic mice strains differing by the allele of Nramp1. IFN-gamma gene expression in vivo was studied by quantitative reverse transcription-PCR performed from the spleen of mice challenged by Salmonell...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/s1286-4579(01)01419-8
更新日期:2001-07-01 00:00:00
abstract::Amoebiasis is responsible for 50000-100000 deaths annually. Invasive amoebic disease begins with the attachment of Entamoeba histolytica trophozoites to colonic mucin, a process mediated by the amoebic Gal/GalNAc lectin. The non-pathogenic counterpart, E. dispar, is morphologically identical but genetically distinct. ...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/s1286-4579(00)01330-7
更新日期:2000-11-01 00:00:00
abstract::While the Vif protein of human immunodeficiency virus type 1 (HIV-1) is essential for viral replication in non-permissive cells, it is rapidly degraded intracellularly. We have previously suggested that the rapid turn-over of Vif is biologically meaningful to prevent detrimental effects of this protein at high express...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2004.04.011
更新日期:2004-07-01 00:00:00
abstract::Two candidate DNA vaccines based on the proteins CFP10 and CFP21 encoded by regions of difference (RDs) of Mycobacterium tuberculosis were evaluated individually and in multivalent combination with the immunodominant protein Ag85B for induction of protective immune responses against experimental tuberculosis. Experime...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.04.025
更新日期:2006-08-01 00:00:00
abstract::We previously showed that human corneal epithelial cells (HCECs) express Toll-like receptors (TLRs), which recognize gram-positive bacteria and respond to Staphylococcus aureus infection by the expression and secretion of proinflammatory cytokines and beta-defensin-2 (hBD2). In this study, we further elucidated the un...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2005.07.006
更新日期:2006-02-01 00:00:00
abstract::Campylobacter jejuni is the predominant cause of antecedent infection in Guillain-Barré syndrome (GBS) or Miller Fisher syndrome (MFS). C. jejuni probably triggers GBS or MFS through molecular mimicry between bacterial sialylated lipo-oligosaccharides (LOS) and gangliosides in peripheral nerve tissue. We investigated ...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2005.06.022
更新日期:2006-01-01 00:00:00
abstract::Flaviviruses enter their host cells by receptor-mediated endocytosis, a well-orchestrated process of receptor recognition, penetration and uncoating. Recent findings on these early steps in the life cycle of flaviviruses are the focus of this review. ...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2010.09.005
更新日期:2011-01-01 00:00:00
abstract::In the present study, we evaluated prophylactic prospective of liposome based DNA vaccine co-expressing Cu-Zn superoxide dismutase (SOD) along with interleukin-18 (IL-18) against experimental murine brucellosis. The immunization schedule involves liposome-mediated delivery of pVsod (encoding SOD of Brucella abortus) a...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2008.05.007
更新日期:2008-08-01 00:00:00
abstract::Fungal allergy including allergic rhinitis, conjunctivitis, bronchial asthma, and allergic bronchopulmonary mycoses results from exposure to spores. In this review we have dealt with the common allergenic fungi and allergens, immunopathogenesis, diagnostic assays, and the possible control of allergy in the future base...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/s1286-4579(00)01264-8
更新日期:2000-07-01 00:00:00
abstract::Gliotoxin is a mycotoxin having a considerable number of immuno-suppressive actions and is produced by several moulds such as Aspergillus fumigatus. In this study, we investigated its toxic effects on human neutrophils at concentrations corresponding to those found in the blood of patients with invasive aspergillosis....
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.10.009
更新日期:2007-01-01 00:00:00
abstract::To obtain monkey-tropic viruses that are more closely related to HIV-1 than the original NL-DT5/NL-DT5R clones, we constructed six vif-chimeric and two site-specific vif-mutant viruses, and examined their growth ability. Different from NL-DT5/NL-DT5R, these viruses did not grow in monkey cells. We monitored the capabi...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2008.06.007
更新日期:2008-08-01 00:00:00
abstract::Helicobacter pylori activates extracellular-signal related (ERK) kinases in gastric epithelial cells, via transactivation of the EGF receptor (EGFR). H. pylori activation of EGFR may be relevant to epithelial hyperproliferation and gastric carcinogenesis. The aim of this study was to develop an 'In-Cell Western' (ICW)...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2007.03.004
更新日期:2007-06-01 00:00:00
abstract::The systemic inflammatory response syndrome (SIRS) is the body's response to an infectious or noninfectious insult. Although the definition of SIRS refers to it as an "inflammatory" response, it actually has pro- and anti-inflammatory components. This review outlines the pathophysiology of SIRS and highlights potentia...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2005.12.016
更新日期:2006-04-01 00:00:00
abstract::Anaerobic bacteria can cause a variety of endogenous infections in children. Because of their fastidious nature, they are difficult to isolate from infectious sites, and are often overlooked. Anaerobic infections can occur in all body sites, including the central nervous system, oral cavity, head and neck, chest, abdo...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/s1286-4579(02)01656-8
更新日期:2002-10-01 00:00:00
abstract::Intensive care unit (ICU)-acquired lower respiratory tract infections include acute tracheobronchitis and hospital-acquired and ventilator-associated pneumonia (VAP). Nosocomial pneumonia is the second most common hospital-acquired infection and the leading cause of death in hospital-acquired infections. The mortality...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2004.12.001
更新日期:2005-02-01 00:00:00
abstract::Francisella tularensis causes the zoonosis tularemia in humans, and inhaled F. tularensis ssp. novicida induces lethal murine tularemia. Transcription of virulence factors in F. novicida is regulated by macrophage growth locus A (mglA), a global regulator required for bacterial replication in macrophages in vitro. We ...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2008.04.008
更新日期:2008-06-01 00:00:00
abstract::Human infection with Toxoplasma gondii is generally asymptomatic in immunocompetent adults while it causes significant morbidity in congenitally infected children. Cell mediated immunity plays the main role in host resistance to T. gondii infection and a Th1 cytokine profile is necessary for protection and control of ...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.10.017
更新日期:2007-02-01 00:00:00