Abstract：
:A cell line of murine osteogenic progenitor cells, Kusa, was established from femoral bone marrow stromal cells with other types of mesenchymal progenitor cells. We characterized two sublines of Kusa (Kusa-A1 and Kusa-O) from several aspects, including the use of an expression profiling system, a cDNA microarray. The original Kusa subline (Kusa-A1) had high alkaline phosphatase activity and high accumulation of calcium deposits in a condition inducing mineralization, with ascorbic acid and beta-glycerophosphate. Kusa-O, a low osteogenic subline of Kusa, had high alkaline phosphatase activity but slow accumulation of calcium deposits even in the inducing condition. These two Kusa sublines differed in the expression of the osteogenic marker genes, osteocalcin and osteopontin, during mineralization. A type of cDNA microarray revealed marked downregulation of gene expression in the inducing condition in both Kusa-A1 and Kusa-O. Another type of high-throughput microarray was performed to examine the difference in gene expression patterns between Kusa-A1 and Kusa-O. By this analysis, periostin, which would be involved in a stage of osteogenesis, was low in Kusa-A1. On the contrary, Myocyte enhancer factor 2C (MEF2C), a myogenic transcriptional factor, was high in Kusa-A1, although no expression of any other myogenic genes was shown.
journal_name
J Bone Miner Metabjournal_title
Journal of bone and mineral metabolismauthors
Kawashima N,Shindo K,Sakamoto K,Kondo H,Umezawa A,Kasugai S,Perbal B,Suda H,Takagi M,Katsube Kdoi
10.1007/s00774-004-0550-ysubject
Has Abstractpub_date
2005-01-01 00:00:00pages
123-33issue
2eissn
0914-8779issn
1435-5604journal_volume
23pub_type
杂志文章abstract：:Bone loss varies according to disease and age and these variations affect bone cells and extracellular matrix. Osteoporosis rat models are widely investigated to assess mechanical and structural properties of bone; however, bone matrix proteins and their discrepant regulation of diseased and aged bone are often overlo...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-017-0844-5
更新日期：2018-05-01 00:00:00
abstract：:Senile or age-related/dependent osteoporosis is caused by reduced bone formation, rather than increased bone resorption as in postmenopausal osteoporosis. Here we review genetically engineered mouse models with defects in osteoblastic proliferation or differentiation with focus on IGF signaling and stem cells. Model m...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章,评审
doi：10.1007/s00774-004-0517-z
更新日期：2004-01-01 00:00:00
abstract：INTRODUCTION:Previous studies have reported that the peak in lean body mass (LBM) precedes the peak in bone mineral content (BMC). However, it is unknown whether the trunk region growth is similar. MATERIALS AND METHODS:We investigated the difference between pubertal peak age in the increase of LBM in the trunk (trunk...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-019-01060-6
更新日期：2020-05-01 00:00:00
abstract：:Digital X-ray radiogrammetry (DXR) is a computer-assisted diagnosis technique for quantifying cortical hand bone mineral density (BMD) as well as the metacarpal index (MCI) in the metacarpal bones from radiographs. The objective was to compare DXR-BMD and DXR-MCI between healthy individuals and patients with rheumatoi...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-016-0741-3
更新日期：2017-03-01 00:00:00
abstract：:In this study, we focused on collagen metabolism as a factor involved in menatetrenone (MK-4)-related improvement in bone quality. Using rats with a congenital ascorbic acid (AA) deficiency, osteogenic disorder Shionogi (ODS) rats, we established a model in which abnormal collagen metabolism reduced bone mechanical pr...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-009-0069-3
更新日期：2009-01-01 00:00:00
abstract：:Disturbed zinc (Zn) homeostasis in mammals is mainly characterized by impaired bone generation accompanied with growth retardation. However, the underlying mechanisms that determine how Zn controls bone homeostasis remain to be defined. Zn homeostasis is tightly controlled by Zn transporter families. Recent studies ha...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章,评审
doi：10.1007/s00774-012-0409-6
更新日期：2013-03-01 00:00:00
abstract：:We have retrospectively studied postmenopausal elderly Japanese women (n = 288; age range, 60-75 years, 65.8 +/- 4.5 [mean +/- SD]) for the evaluation of biochemical measurements in assessment of bone mass and vertebral fracture, comparing with several bone mineral measurements and quantitative ultrasound (QUS) measur...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s007740050107
更新日期：2000-01-01 00:00:00
abstract：:In this study, we tested the interleukin-6 (IL-6) gene as an important candidate gene for its linkage and association with the variation of bone phenotypes (bone mineral density [BMD] and bone size) in young Chinese female subjects. We genotyped the IL-6 gene at the -634C/G restriction fragment length polymorphism (RF...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-004-0607-y
更新日期：2005-01-01 00:00:00
abstract：INTRODUCTION:Aromatase inhibitors are known to accelerate bone loss in patients with breast cancer. However, how much AIs affect the efficacy of antiresorptive agents has not been studied. The study aimed to compare the effect of alendronate on bone mineral density (BMD) between patients with and without AI treatment. ...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-020-01111-3
更新日期：2020-09-01 00:00:00
abstract：:Inactivating mutations and/or deletions of PHEX ( Phosphate-regulating gene with Homologies to Endopeptidase on the X chromosome) are responsible for X-linked hypophosphatemic rickets in humans. In the present study, three Drosophila PHEX homologues (dPHEX-1, -2, -3) were isolated by the screening of a Drosophila cDNA...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-003-0440-8
更新日期：2004-01-01 00:00:00
abstract：:After acute spinal cord injury (SCI), rapid depletion of the sublesional skeleton occurs, particularly at the distal femur and proximal tibia. Subsequently, fragility fractures of the knee may occur. We determined the efficacy of zoledronic acid to prevent sublesional bone mineral density (BMD) loss at 6 and 12 months...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-014-0602-x
更新日期：2015-07-01 00:00:00
abstract：:Serum 25-hydroxyvitamin D (25-OHD) concentrations are thought to accurately reflect vitamin D stores, and vitamin D deficiency causes secondary hyperparathyroidism, irreversible bone loss, and increased risk of fracture. Recent studies suggest that decrease of serum 25-OHD level in mothers could increase the risk of p...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-011-0264-x
更新日期：2011-09-01 00:00:00
abstract：:High serum homocysteine (HCY) and indirectly deficiency of folate and/or vitamin B(12) stimulate bone resorption and adversely affect collagen cross-linking. The aim of this study was the evaluation of serum levels of HCY, folate and vitamin B(12) in patients with Paget's disease of bone (PDB) and the effect of zoledr...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-009-0131-1
更新日期：2010-05-01 00:00:00
abstract：:The aim of the study was to assess body composition in subjects with phenylketonuria (PKU). Forty-five patients aged 13.8 ± 5.2 years were evaluated. Among them, 15 patients had not reached sexual maturity, showing normal serum values of phenylalanine (Phe) (subgroup 1), and 30 subjects were sexually mature (Tanner 5 ...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-010-0216-x
更新日期：2011-03-01 00:00:00
abstract：INTRODUCTION:Etelcalcetide binds to the extracellular domain of the calcium-sensing receptor (CaSR), while cinacalcet binds to the 7-transmembrane domain of the CaSR; however, it is unknown, whether etelcalcetide has similar effects to cinacalcet on parathyroid hormone (PTH) secretion. MATERIALS AND METHODS:The PTH-ca...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-020-01169-z
更新日期：2020-11-16 00:00:00
abstract：:The objective of this study was to record the clinical course of osteoporosis over time in Japanese women. We hypothesized that successful control of acute pain associated with osteoporosis will allow better treatment for health-related problems. To confirm this working hypothesis, we developed the Japan Questionnaire...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-013-0458-5
更新日期：2013-11-01 00:00:00
abstract：:Stimulation of osteoblast proliferation and differentiation is important for the in vivo bone-bonding ability of biomaterials. Previous in vitro studies have used biochemical assays to analyze osteoblast-specific gene expression in cultured osteoblasts. In this study, we generated transgenic mice harboring a monomeric...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-013-0544-8
更新日期：2014-11-01 00:00:00
abstract：:Low levels of vitamin D nutrition (i.e., vitamin D insufficiency), which potentially cause osteoporosis, have been recognized as a common health problem for elderly people in European and North American countries, but there have been no studies on the prevalence of vitamin D insufficiency in Japan until recently. The ...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章,评审
doi：10.1007/s00774-005-0637-0
更新日期：2006-01-01 00:00:00
abstract：:Abeta-2M-amyloidosis is a type of systemic amyloidosis that is specifically seen in patients with chronic kidney diseases. The precursor protein of Abeta-2M-amyloid fibril is beta2-microglobulin, and its elevated serum level is the main cause of Abeta-2M-amyloidosis in patients with kidney failure. However, the precis...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章,评审
doi：10.1007/s00774-005-0669-5
更新日期：2006-01-01 00:00:00
abstract：:It has been suggested that there are several possible linkages between vascular calcification and osteoporosis. In addition, the processes of vascular calcification may have a common etiology with bone formation. Thus, we hypothesized that the serum levels of bone metabolic markers would be different between osteoporo...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-004-0528-9
更新日期：2004-01-01 00:00:00
abstract：:The aim of this study was to explore the effects of α-zearalanol (α-ZAL) on the proliferation of mouse bone-marrow-derived mesenchymal stem cells (BMSCs) and their differentiation into osteoblasts. Six- to eight-week-old BALB/C mice were used either as recipients or as bone marrow donors. BMSCs were isolated and colle...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-015-0659-1
更新日期：2016-03-01 00:00:00
abstract：:The prevalence and pattern of joint involvement in radiographic hand osteoarthritis (OA) have been reported in Western populations, but similar data are lacking for Japanese. We examined this issue in 551 Japanese women aged > or = 40 years. Radiographs were obtained of both hands and graded according to the Kellgren-...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-006-0693-0
更新日期：2006-01-01 00:00:00
abstract：:Osteopathic changes sometimes occur in patients with complex regional pain syndrome (reflex sympathetic dystrophy and causalgia). We aimed to investigate whether such osteopathic changes occurred in rats with chronic constriction injury (CCI) of the sciatic nerve. A CCI of the sciatic nerve was established in a unilat...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s007740200012
更新日期：2002-01-01 00:00:00
abstract：:Rodent hindlimb unloading (HU) by tail-suspension is a model to investigate disuse-induced bone loss in vivo. Previously, we have shown that osteopontin (OPN, also known as Spp1) is required for unloading-induced bone loss. However, how unloading affects OPN expression in the body is not fully understood. Here, we exa...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-014-0568-8
更新日期：2015-01-01 00:00:00
abstract：:Until now, the effects of phytoestrogen on bone in both women and ovarian hormone-deficient animal models of osteoporosis have remained uncertain. We have aimed here to investigate the effect of genistein (GEN) on trabecular bone quality in ovariectomized (OVX) rats. Forty 7-month-old female Sprague-Dawley rats were r...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-007-0830-4
更新日期：2008-01-01 00:00:00
abstract：:Vertebral fractures are the most common complication associated with osteoporosis. The definition of prevalent or incident vertebral fractures is important in epidemiological evaluation and in clinical trials of osteoporosis treatment. There have been few reports regarding the morphometric cutoff criteria for incident...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-003-0457-z
更新日期：2004-01-01 00:00:00
abstract：:We previously reported that simvastatin induces estrogen receptor-alpha (ERα) in murine bone marrow stromal cells in vitro. In this study, we investigated the effect of simvastatin on ERα expression in bone and uterus in ovariectomized (OVX) rats and evaluated bone mass, bone strength, and uterine wet weight. Three-mo...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-010-0231-y
更新日期：2011-07-01 00:00:00
abstract：:Skull changes are poorly described in vitamin D insufficiency [serum 25-hydroxyvitamin D (s-25(OH)D) 25-50 nmol/L]. We aimed to investigate factors associated with cranial anthropometrics in infants, especially s-25(OH)D. In infants 2.5-6 months old from the Odense Child Cohort, associations between cord and pregnancy...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-017-0881-0
更新日期：2018-11-01 00:00:00
abstract：:The purpose of this study was to investigate the association of the Singh index, cortical thickness index (CTI) and calcar-to-canal (CC) ratio with bone mineral density (BMD) as well as their relationship with physical parameters [age, gender, height, and body mass index (BMI)] in order to assess their potential as an...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-012-0348-2
更新日期：2012-09-01 00:00:00
abstract：:Aromatase inhibitors (AIs) are the gold standard therapy for breast cancer in postmenopausal women. AI suppresses the conversion of androgens to estrogens; however, this results in osteopenia, osteoporosis, and bone fracture, thus reducing the patient's quality of life. The use of adjuvant denosumab reduces the risk o...
journal_title：Journal of bone and mineral metabolism
pub_type： 临床试验,杂志文章
doi：10.1007/s00774-018-00985-8
更新日期：2019-09-01 00:00:00
