Abstract:
:Thiazolidinediones, such as pioglitazone, seem to exert direct antiatherosclerotic and antirestenotic effects on type 2 diabetes, in part due to an induction of vascular smooth muscle cell (VSMC) apoptosis. We aimed to study the role of transforming growth factor (TGF)-beta in rat aortic VSMC. Pioglitazone at 100 micromol/l increased apoptosis without affecting DNA synthesis, and this effect was reversed by an anti-TGF-beta1 antibody. Extracellular TGF-beta1 levels were rapidly increased after treatment with pioglitazone in a peroxisome proliferator-activated receptor (PPAR)-gamma-dependent mechanism because this secretion was blocked by the PPAR-gamma inhibitor GW9662. Pioglitazone subsequently increased the nuclear recruitment of phospho-Smad2, without any effect on protein expression. According to our results, we propose that the apoptotic effect of pioglitazone on VSMC depends on the following sequence: PPAR-gamma activation, TGF-beta1 release, and selective phospho-Smad2 nuclear recruitment. Management of Smad signaling on VSMC might provide future clinical benefits in vascular diseases.
journal_name
Diabetesjournal_title
Diabetesauthors
Redondo S,Ruiz E,Santos-Gallego CG,Padilla E,Tejerina Tdoi
10.2337/diabetes.54.3.811subject
Has Abstractpub_date
2005-03-01 00:00:00pages
811-7issue
3eissn
0012-1797issn
1939-327Xpii
54/3/811journal_volume
54pub_type
杂志文章相关文献
DIABETES文献大全abstract::A total of 32 pairs of obese fathers or mothers and their obese offsprings, aged 20 yr or younger, from 25 families with established hyperinsulinemic obesity aged 11-20 yr equally showed an insulin hyperresponse to glucose, the offspring 10 yr or younger revealed a rather normal insulin response, despite the presence ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.30.1.14
更新日期:1981-01-01 00:00:00
abstract::Ectopic lipids in peripheral tissues have been implicated in attenuating insulin action in vivo. The botanical extract of Artemisia dracunculus L. (PMI 5011) improves insulin action, yet the precise mechanism is not known. We sought to determine whether the mechanism by which PMI 5011 improves insulin signaling is thr...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db11-0396
更新日期:2012-03-01 00:00:00
abstract::Liver IGF-1 deficient (LID) mice demonstrate a 75% reduction in circulating IGF-1 levels and a corresponding fourfold increase in growth hormone (GH) levels. At 16 weeks of age, LID mice demonstrate, using the hyperinsulinemic-euglycemic clamp, insulin insensitivity in muscle, liver, and fat tissues. In contrast, mice...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.52.10.2483
更新日期:2003-10-01 00:00:00
abstract::The selective impairment of glucose-induced insulin secretion in NIDDM can be attributed to defects in the glucose-signaling system. An alteration in the activity of phosphofructokinase (PFK), a key enzyme in the glycolytic pathway, may play a role in the abnormal glucose-induced insulin secretion. In this study, we e...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.46.9.1414
更新日期:1997-09-01 00:00:00
abstract::Progression to clinical type 1 diabetes varies among children who develop β-cell autoantibodies. Differences in autoantibody patterns could relate to disease progression and etiology. Here we modeled complex longitudinal autoantibody profiles by using a novel wavelet-based algorithm. We identified clusters of similar ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db18-0594
更新日期:2019-01-01 00:00:00
abstract::An A-to-G transition in the mitochondrial tRNALeu(UUR) gene at base pair 3243 has been shown to be associated with the maternally transmitted clinical phenotype of NIDDM and sensorineural hearing loss in white and Japanese pedigrees. We have detected this mutation in 25 of 50 tested members of five white French pedigr...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.45.4.478
更新日期:1996-04-01 00:00:00
abstract::Muscle insulin sensitivity for stimulating glucose uptake is enhanced in the period after a single bout of exercise. We recently demonstrated that AMPK is necessary for AICAR, contraction, and exercise to enhance muscle and whole-body insulin sensitivity in mice. Correlative observations from both human and rodent ske...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db18-0769
更新日期:2019-09-01 00:00:00
abstract::Mitochondrial injury is uniformly observed in several murine models as well as in individuals with diabetic kidney disease (DKD). Although emerging evidence has highlighted the role of key transcriptional regulators in mitochondrial biogenesis, little is known about the regulation of mitochondrial cytochrome c oxidase...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db17-0958
更新日期:2018-11-01 00:00:00
abstract::Lymphocyte antibodies have been described in autoimmune disorders, including insulin-dependent diabetes mellitus (IDDM). We have developed a quantitative method to measure autoantibodies directed against T-lymphocytes, based on two-color fluorescence labeling of Wistar mononuclear cells and analysis of fluorescence by...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.43.1.137
更新日期:1994-01-01 00:00:00
abstract::Insulin and glucagon release and insulin sensitivity were investigated in patients with glucokinase deficiency. Five subjects with a missense mutation (Glu256Lys) were studied. They were compared with six healthy subjects with low insulin response but normal glucose tolerance. Insulin and glucagon levels were measured...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.43.12.1402
更新日期:1994-12-01 00:00:00
abstract::Low-dose antithymocyte globulin (ATG) plus pegylated granulocyte colony-stimulating factor (G-CSF) preserves β-cell function for at least 12 months in type 1 diabetes. Herein, we describe metabolic and immunological parameters 24 months following treatment. Patients with established type 1 diabetes (duration 4-24 mont...
journal_title:Diabetes
pub_type: 杂志文章,随机对照试验
doi:10.2337/db16-0823
更新日期:2016-12-01 00:00:00
abstract::Obesity is a strong risk factor for the development of type 2 diabetes. We have previously reported that in adipose tissue of obese (ob/ob) mice, the expression of adipogenic genes is decreased. When made genetically obese, the BTBR mouse strain is diabetes susceptible and the C57BL/6J (B6) strain is diabetes resistan...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.52.3.688
更新日期:2003-03-01 00:00:00
abstract::We tested the hypothesis that green tea prevents diabetes-related tissue dysfunctions attributable to oxidation. Diabetic rats were treated daily with tap water, vitamins C and E, or fresh Japanese green tea extract. After 12 months, body weights were decreased, whereas glycated lysine in aorta, tendon, and plasma wer...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.54.2.517
更新日期:2005-02-01 00:00:00
abstract::The IGF system is increasingly implicated in the development of cardiovascular disease. The effects of circulating IGFs on the vasculature are largely modulated by IGFBPs, which control their access to cell-surface IGF receptors. IGFBP-1 has been proposed as the acute regulator of IGF bioavailability because of its me...
journal_title:Diabetes
pub_type: 杂志文章,多中心研究
doi:10.2337/diabetes.51.8.2629
更新日期:2002-08-01 00:00:00
abstract::Peptide-YY (PYY) is secreted from endocrine L-cells of the gastrointestinal tract in response to caloric ingestion and may mediate postprandial satiety through the hypothalamic neuropeptide Y2 receptor (Y2R). We examined whether variants in the genes encoding PYY and Y2R might be associated with obesity-related phenot...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.53.9.2461
更新日期:2004-09-01 00:00:00
abstract::The significance of the portohepatic glucosensors for counterregulation in deep hypoglycemia (i.e., glycemia < 2.8 mM) was studied in chronically cannulated male mongrel dogs in the conscious state. A total of 16 experiments were carried out on 6 dogs using the liver clamp technique under hyperinsulinemic conditions (...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.43.8.1052
更新日期:1994-08-01 00:00:00
abstract::One subtype of maturity-onset diabetes of the young (MODY)-3 results from mutations in the gene encoding hepatocyte nuclear factor (HNF)-1 alpha. We generated transgenic mice expressing a naturally occurring dominant-negative form of human HNF-1 alpha (P291fsinsC) in pancreatic beta-cells. A progressive hyperglycemia ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.51.1.114
更新日期:2002-01-01 00:00:00
abstract::The development of autoimmune diabetes in NOD mice results from selective destruction of beta-cells by a T-cell-dependent autoimmune process. However, the mechanisms that control the generation of beta-cell cytotoxic T-cells in vivo are poorly understood. We recently established 8.3-T-cell receptor (TCR)-beta transgen...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.48.1.34
更新日期:1999-01-01 00:00:00
abstract::Immune modulation of pancreatic inflammation induces recovery from type 1 diabetes (T1D), but remission was not durable, perhaps because of an inability to sustain the formation and function of new pancreatic β-cells. We have previously shown that Ig-GAD2, carrying GAD 206-220 peptide, induced in hyperglycemic mice im...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db12-1281
更新日期:2013-08-01 00:00:00
abstract:OBJECTIVE:Atherosclerotic cardiovascular disease is the leading cause of death among people with diabetes. Generation of oxidized LDLs and reduced nitric oxide (NO) availability because of endothelial NO synthase (eNOS) dysfunction are critical events in atherosclerotic plaque formation. Biochemical mechanism leading f...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db09-0167
更新日期:2009-10-01 00:00:00
abstract::An in vivo microdialysis technique was used to study adrenal medullary function in 6 euglycemic and 6 hyperglycemic anesthetized Sprague-Dawley rats exposed to hypoxia. After stabilization of an adrenal dialysis probe, dialyzable adrenal epinephrine and norepinephrine were measured in response to 15 min of 7.8% oxygen...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.43.5.645
更新日期:1994-05-01 00:00:00
abstract::GAD autoantibodies (GADAs) are sensitive markers of islet autoimmunity and type 1 diabetes. They form the basis of robust prediction models and are widely used for the recruitment of subjects at high risk of type 1 diabetes to prevention trials. However, GADAs are also found in many individuals at low risk of diabetes...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db14-1693
更新日期:2015-09-01 00:00:00
abstract::A polymorphic region 5' to the human insulin gene has been associated with diabetes in earlier studies. This polymorphic region is composed of tandem repeats that fall into 3 general size classes, designated class 1 (600 base pairs), class 2 (1300 base pairs), and class 3 (2500 base pairs). Frequencies of these classe...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.34.5.433
更新日期:1985-05-01 00:00:00
abstract::To evaluate possible advantages of human insulin of recombinant DNA origin (HI) in the treatment of diabetic patients, we compared cellular and humoral immunoreactivities of HI and porcine insulin (PI). Anti-insulin IgE bound equal amounts of 125I-HI and 125I-PI. There was no difference between HI- and PI-stimulated l...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.6.516
更新日期:1983-06-01 00:00:00
abstract::Recently, missense mutations upstream of preproinsulin's signal peptide (SP) cleavage site were reported to cause mutant INS gene-induced diabetes of youth (MIDY). Our objective was to understand the molecular pathogenesis using metabolic labeling and assays of proinsulin export and insulin and C-peptide production to...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db11-0878
更新日期:2012-04-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) has been suggested to play a role in the pathogenesis of diabetic vascular complications. In the present study, we investigated whether expression of monocyte chemoattractant protein-1 (MCP-1), a chemokine that has been proposed to recruit leukocytes to sites of inflammation, ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.48.5.1131
更新日期:1999-05-01 00:00:00
abstract::Although ATP-binding cassette transporter A1 (ABCA1) is well known for its role in cholesterol efflux and HDL formation, it is expressed in various tissues, where it may have different functions. Because hypoalphalipoproteinemia is highly prevalent in Mexico, we screened the ABCA1 coding sequence in Mexican individual...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db06-0905
更新日期:2007-07-01 00:00:00
abstract:OBJECTIVE:Physiologically elevated insulin concentrations promote access of macromolecules to skeletal muscle in dogs. We investigated whether insulin has a stimulating effect on the access of macromolecules to insulin-sensitive tissues in humans as well. RESEARCH DESIGN AND METHODS:In a randomized, controlled trial, ...
journal_title:Diabetes
pub_type: 杂志文章,随机对照试验
doi:10.2337/db07-0238
更新日期:2007-09-01 00:00:00
abstract::The experiments reported here investigate the effects of two conditions of elevated lactogen activity on the threshold of glucose stimulation of insulin secretion and suprathreshold, glucose-stimulated insulin secretion in the isolated, perfused rat pancreas. In both the tumor-bearing animals and pregnant animals, the...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.34.4.337
更新日期:1985-04-01 00:00:00
abstract::Type 1 diabetes presents clinically with overt hyperglycemia resulting from progressive immune-mediated destruction of pancreatic β-cells and associated metabolic dysfunction. Combined genetic and immunological studies now highlight deficiencies in both the interleukin-2 (IL-2) receptor and its downstream signaling pa...
journal_title:Diabetes
pub_type: 杂志文章,评审
doi:10.2337/db11-1213
更新日期:2012-01-01 00:00:00