Evaluation of opa-based real-time PCR for detection of Neisseria gonorrhoeae.

Abstract:

OBJECTIVES:Detection of Neisseria gonorrhoeae by commercial and in-house-based assays has been hampered by false-positive and false-negative results. The current study describes a sensitive and specific real-time 5'-nuclease PCR assay targeting a 90-bp region of the multicopy opa gene. GOAL:To evaluate the sensitivity and specificity of this assay in detection of gonococcus. STUDY:Sensitivity and specificity were determined by testing a panel of 173 microorganisms. In addition, 135 clinical samples previously evaluated by 4 nucleic acid amplification methods were also tested. RESULTS:A sensitivity of 1 copy per reaction was achieved. Positive results were only obtained for N gonorrhoeae strains including 20 cppB-negative strains. Overall, 134 of 135 clinical sample results agreed with the consensus nucleic amplification methods. CONCLUSION:This study demonstrates opa-based target can be used as an accurate and rapid PCR assay for the detection of N gonorrhoeae in clinical specimens.

journal_name

Sex Transm Dis

authors

Tabrizi SN,Chen S,Tapsall J,Garland SM

doi

10.1097/01.olq.0000154495.24519.bf

subject

Has Abstract

pub_date

2005-03-01 00:00:00

pages

199-202

issue

3

eissn

0148-5717

issn

1537-4521

pii

00007435-200503000-00012

journal_volume

32

pub_type

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