Abstract:
:The capabilities of porcine adrenal cortex mitochondria to oxidize glycerol-3-phosphate (GP) were studied. In comparison with bovine adrenal cortex mitochondria, porcine mitochondria oxidized GP about three times more actively (18.9 vs 6.1 nmol O(2)/min per mg protein in the presence of ADP) and the activity of mitochondrial glycerol-3-phosphate dehydrogenase was about four times higher (33.4 vs 8.2 nmol/min per mg protein). In porcine adrenal cortex mitochondria we found similar values for succinate and GP oxidation both in the absence and presence of ADP or deoxycorticosterone (DOC). Rotenone sensitivity of DOC stimulation of GP oxidation indicated that porcine adrenal cortex mitochondria are able to oxidize GP and thus to generate NADPH from GP, presumably via reverse electron transport followed by energy-dependent NADH-NADP transhydrogenation.
journal_name
Acta Biochim Poljournal_title
Acta biochimica Polonicaauthors
Popinigis J,Antosiewicz J,Kaczor JJ,Rauchová H,Lenaz Gdoi
0451041075subject
Has Abstractpub_date
2004-01-01 00:00:00pages
1075-80issue
4eissn
0001-527Xissn
1734-154Xjournal_volume
51pub_type
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