Abstract:
BACKGROUND:The haptoglobin (Hp) 2-2 phenotype has been known to have less antioxidative activity than Hp 1-1 and Hp 2-1. However, few have reported the relationship between oxidative stress and Hp phenotypes by using oxidized LDL. The relationship between Hp phenotypes and oxidative stress was therefore investigated in healthy adults. METHODS:The serum Hp concentrations, albumin, uric acid, iron, L-ascorbic acid, total antioxidant status and oxidized LDL concentrations were measured and analysed in 200 healthy Korean men and women with Hp 2-1 and Hp 2-2 phenotypes. RESULTS:The serum concentrations of Hp were significantly higher in the Hp 2-1 compared with the Hp 2-2 phenotype. However, serum concentrations of total antioxidant status and oxidized LDL did not show any statistical differences in either sex. The L-ascorbic acid concentrations in serum showed lower values in the Hp 2-2 compared with the Hp 2-1 phenotype, for both sexes (P < 0.05). CONCLUSIONS:The concentration of L-ascorbic acid, the first line antioxidant, was lower in the Hp 2-2 than in the Hp 2-1 phenotype; this did not significantly affect the total antioxidant status, and there was no significant difference in oxidized LDL for either group in healthy adults.
journal_name
Ann Clin Biochemjournal_title
Annals of clinical biochemistryauthors
Lee YW,Min WK,Chun S,Lee W,Park H,Lee YK,Lee DH,Son JEdoi
10.1258/0004563042466703subject
Has Abstractpub_date
2004-11-01 00:00:00pages
485-7issue
Pt 6eissn
0004-5632issn
1758-1001journal_volume
41pub_type
杂志文章abstract:BACKGROUND:Supported liquid extraction (SLE) techniques are relatively new compared to other sample preparation approaches such as solid phase extraction (SPE), liquid-liquid extraction (LLE) and protein precipitation (PPE). We investigated the use of SLE as an alternative to SPE for the liquid chromatography tandem ma...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563213480758
更新日期:2013-09-01 00:00:00
abstract::A simultaneous deterioration in performance of several radioimmunoassays suggested that equipment was malfunctioning. Replicate counts from an automated gamma counter showed unacceptable variation during a 24-hour period; this variation was detected using Trigg's technique, a sensitive method for trend analysis, and w...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327801500126
更新日期:1978-03-01 00:00:00
abstract::Seventeen laboratories in the British Isles participated in a study to compare six different commercially available immunoassays for serum lipoprotein(a) (Lp(a)) and to establish reasons for the variations in the measurement of serum Lp(a) concentrations. Pooled serum was distributed neat and after dilution at a centr...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329603300110
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND:In the enzyme reaction for the determination of the unbound (free) bilirubin concentration by glucose oxidase and peroxidase, materials with low affinity for serum protein are reactive. The influence of these materials on the determination of serum unbound bilirubin was investigated. METHODS Serum samples fr...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456320203900606
更新日期:2002-11-01 00:00:00
abstract:BACKGROUND:Our study aims to explore the effect of serum long non-coding RNA (lncRNA) H19 level on the long-term prognosis of endoscopic keyhole surgery or craniotomy for glioma. METHODS:A total of 264 glioma patients were selected. Patients were randomly divided into the Craniotomy-high H19 group, the Craniotomy-low ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563220941888
更新日期:2020-09-01 00:00:00
abstract::The presence of antibodies in some patients' serum has long been known to be a potential source of interference in immunoassays, as shown by numerous case reports. These often appear after the introduction of a new analyte (e.g. troponin) and then decrease in number as the topic becomes exhausted. This highlights the ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1258/000456302760042128
更新日期:2002-07-01 00:00:00
abstract::Auto-antibodies to oxidized low-density lipoprotein (ox-LDL) are thought to play a pivotal role in the pathogenesis of atherosclerosis. This study investigates the value of auto-antibodies to ox-LDL as a predictive marker of atherosclerosis in patients with both homozygous and heterozygous familial hypercholesterolaem...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563001899177
更新日期:2000-03-01 00:00:00
abstract:BACKGROUND:Urinary excretion of the low molecular weight protein cystatin C is a marker of renal disorders and a good predictor of the severity of acute tubular necrosis. We evaluated the measurement of urinary cystatin C and determined its reference range. METHODS:Measurement of urinary cystatin C by particle-enhance...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456304322879980
更新日期:2004-03-01 00:00:00
abstract:BACKGROUND:Measurement of aldosterone and/or renin is essential to aid the differential diagnosis of secondary hypertension, guide strategy for therapeutic management of hypertension and assess adequacy of mineralocorticoid replacement. AIM:The objective was to establish normative data for aldosterone and renin using ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563215603401
更新日期:2016-05-01 00:00:00
abstract::A group of international experts prepared two lists of drugs with their serum/plasma and urine concentrations, which should be used when evaluating the performance of a new laboratory method. The two lists were verified by running in vitro interference studies in three European laboratories on Hitachi instruments. The...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,多中心研究
doi:10.1258/0004563011900696
更新日期:2001-07-01 00:00:00
abstract::17-Hydroxyprogesterone (17-OHP) is an intermediate steroid in the adrenal biosynthetic pathway from cholesterol to cortisol and is the substrate for steroid 21-hydroxylase. An inherited deficiency of 21-hydroxylase leads to greatly increased serum concentrations of 17-OHP, while the absence of cortisol synthesis cause...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563214529748
更新日期:2014-07-01 00:00:00
abstract::The total cost of a haematology and clinical chemistry laboratory, including external and hospital costs, was studied in a district general hospital in The Netherlands. The main conclusion is that the number of samples analysed is the most important cost-setting factor, but that reduction in the number of requests doe...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328402100206
更新日期:1984-03-01 00:00:00
abstract::An audit of troponin measurement protocols in use in Wales showed significant variations in practice with no consensus on analytical methods, or in the selection of a decision limit for the diagnosis of myocardial damage. Peer review data on assay imprecision at concentrations approaching the analytical limit of detec...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,多中心研究
doi:10.1258/0004563054890006
更新日期:2005-09-01 00:00:00
abstract:BACKGROUND:Golgi protein-73 (GP73) is a newly identified candidate serum marker for liver diseases. The utility of this biomarker remains limited, largely due to the lack of quantitative information. The aims of this study were to quantify serum GP73 (sGP73) in healthy individuals and in patients with liver diseases, a...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2008.008088
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Sickle cell disease is a heterogenous disorder characterized by an abnormal haemoglobin and sickling phenomena of red cells. It is prevalent among certain nomadic tribes in Sudan. Painful, aplastic and haemolytic crises mark sickle cell anaemia. Haemoglobin S (HbS) is detected using haemoglobin electrophores...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2008.008155
更新日期:2009-03-01 00:00:00
abstract::Experience in the use of an in-house urinary free catecholamine assay for the investigation of possible neural crest tumours in a District General Hospital laboratory is described. Elevated excretion of catecholamines and vanillylmandelic acid was found in a number of cases including phaeochromocytoma and neuroblastom...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328702400512
更新日期:1987-09-01 00:00:00
abstract::Smoking is a major risk factor for periodontal disease, causing bone and tooth loss. Effective management of many oral conditions requires accurate information about smoking. We describe a disposable, 10-min, near-patient saliva test to monitor cigarette smoking. A plastic device contains the dried reagents to measure...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563001899717
更新日期:2000-09-01 00:00:00
abstract::The most widely used methods for the assay of total bilirubin in serum are based on a coupling reaction with various diazo dyes in the presence of an accelerating agent. The 'direct' reaction, without accelerator, gives a good estimate of the conjugated and protein bound (delta) species of bilirubin only if carefully ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1177/000456329102800202
更新日期:1991-03-01 00:00:00
abstract::A comparison has been made between three methods for measuring capillary blood glucose collected on filter paper, including a new method using a Beckman glucose analyser 2. The methods are suitable for use with capillary blood spots collected onto filter paper for monitoring the control of diabetic patients. Their acc...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328201900105
更新日期:1982-01-01 00:00:00
abstract::The development and evaluation in routine clinical practice of a simple, rapid, high-throughput, immunoradiometric assay (IRMA) for alpha-foetoprotein (AFP) in serum and amniotic fluid is described. The assay uses readily available reagents; a radioiodinated mouse monoclonal antibody produced by the authors and the im...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328702400411
更新日期:1987-07-01 00:00:00
abstract::Inherited metabolic disorders account for a small but significant number of sudden unexplained deaths in neonates, infants and occasionally older children. In particular, inherited disorders of fatty acid oxidation may closely mimic sudden infant death syndrome. Post-mortem investigations offer the final opportunity t...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1258/0004563041201473
更新日期:2004-07-01 00:00:00
abstract::We examined the reactivity of human serum albumin-bound bilirubin and its photoisomers as substrates for a direct bilirubin assay using bilirubin oxidase. The reduction of (EZ)-cyclobilirubin reached 100% 5 min after addition of the enzyme at any pH tested (3.5-7.4) in 0.1 mol/L phosphate buffer, whereas the reduction...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456320003700404
更新日期:2000-07-01 00:00:00
abstract::A rapid gas liquid chromatography method has been developed which is suitable for emergency estimations of paracetamol in overdose. The method is compared with a standard ultraviolet method. ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327501200102
更新日期:1975-01-01 00:00:00
abstract::The spectral interference caused by haemoglobin in the calcium-cresolphthalein complexone reaction was investigated. The results show that the nature of the haemoglobin derivative in the reaction mixture influences the degree of interference; in turn, the derivative formed depends on the order in which the sample and ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329002700212
更新日期:1990-03-01 00:00:00
abstract::A rapid vacuum-driven procedure, using pre-treated Sep-Pak C18 cartridges, has been developed for the simultaneous extraction of arginine vasopressin (AVP) and atrial natriuretic peptide (ANP) from plasma. Non-specific interference was removed by fractional elution with an aqueous methanol/trifluoroacetic acid (TFA) m...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329202900606
更新日期:1992-11-01 00:00:00
abstract::Heterophilic antibodies are well described, but poorly appreciated interferents and is often not a recognized problem affecting most immunoassays. We report a patient presented with ectopic Cushing's syndrome (CS), but repeated plasma adrenocorticotropic hormone (ACTH) concentrations conducted by immunoassay were inap...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563214520751
更新日期:2014-07-01 00:00:00
abstract::Acid-urea extract of rat brain was examined by glucagon-like peptide-1 (GLP-1) specific radioimmunoassay. A single peak was observed which co-eluted with GLP-1(7-36)amide on gel filtration and anion exchange chromatography. In contrast, GLP-1(1-37) was not detected under our experimental conditions. The fact that GLP-...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328902600214
更新日期:1989-03-01 00:00:00
abstract::Erythrocyte and fasting plasma magnesium were assayed by a dyebinding colorimetric method using the metallochromic dye, Calmagite, in 51 diabetic Nigerians and 25 controls. Erythrocyte magnesium concentrations were similar in the diabetics and the controls. Fasting plasma magnesium concentrations were significantly lo...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328902600111
更新日期:1989-01-01 00:00:00
abstract::A 19-year-old man who developed extensive oesophageal lye (Alkali) stricture and received long-term enteral nutrition (eight months) with a jejunostomy tube developed macrocytic anaemia (Hb: 41 g/L) with leucopenia (white blood cell [WBC]: 3.0 x 10(9)/L). The patient's serum vitamin B12, folate, iron and liver functio...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563053857879
更新日期:2005-05-01 00:00:00
abstract::Gestational diabetes is a common pregnancy disorder which is generally managed with diet, exercise, metformin or insulin treatment and which usually resolves after delivery of the infant. Identifying and treating gestational diabetes improves maternal and fetal outcomes and allows for health promotion to reduce the mo...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1177/0004563216674743
更新日期:2017-01-01 00:00:00