Abstract:
BACKGROUND:To understand the molecular response of tumor cells to therapeutic ionizing radiation (IR), we previously reported that human breast cancer cells derived following chronic exposure to fractionated ionizing radiation (MCF+FIR) showed a transient radioresistance. MCF+FIR cells also demonstrated increased activity of NF-kappaB, increased expression of the mitochondrial antioxidant enzyme (MnSOD), and increased expression of a cell cycle regulatory protein (Cyclin B1). The present studies were designed to determine the relationship of NF-kappaB, MnSOD and Cyclin B1 expression in cellular adaptive responses to ionizing radiation. MATERIALS AND METHODS:The first intron of the cyclin B1 gene with a putative NF-kappaB element was cloned into the pGL3 luciferase reporter (pGL3CB1EI1). PGL3CB1EI1 and control NF-kappaB luciferase activities were determined in MCF-7 and MCF+FIR cells treated with a single dose of radiation, over expression of the dominant negative mutant IkB (mIkB) or over expression of the SOD2 gene. RESULTS:MCF+FIR cells derived from fractionated IR demonstrated increased transactivation of the pGL3CB1EI1 and NF-kappaB controlled reporter activities, relative to the parental cell line. Transfection of dominant negative mutant IkB that inhibits NF-kappaB nuclear translocation, inhibited pGL3CB1EI1 and NF-kappaB activity, indicating the NF-kappaB dependence of pGL3CB1EI1 mediated transcription. In addition, over expression of the human SOD2 gene (MnSOD) inhibited NF-kappaB and pGL3CB1EI1 activity, indicating that superoxide or some species derived from superoxide may have participated in the up-regulation of reporter activity in response to chronic exposure to fractionated ionizing radiation. These results provide evidence suggesting that a signaling pathway involving NF-kappaB and Cyclin B1 may contribute to adaptive radioresistance induced by chronic exposure to fractionated IR and support the conclusion that MnSOD appears to be a negative regulator of this pathway.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Ozeki M,Tamae D,Hou DX,Wang T,Lebon T,Spitz DR,Li JJsubject
Has Abstractpub_date
2004-09-01 00:00:00pages
2657-63issue
5Aeissn
0250-7005issn
1791-7530journal_volume
24pub_type
杂志文章abstract::Smoking is the primary factor responsible for the epidemic of squamous cell lung cancer (SqLC) and small cell lung cancer (SCLC), however, there have been few studies which reported the relationship between smoking and SCLC in female patients. To examine the relationship between smoking and SCLC and SqLC in female pat...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract::Intratumoral (cervix uterine tumors) distribution of platinum was studied in ten patients after IV administration of CDDP. Sequential tumoral measurement of platinum was carried out 4, 24, and 48 h after the IV infusion. Platinum concentration in the tumor samples ranged from 0 to 29,5 ng/mg tissue. For all patients, ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1985-07-01 00:00:00
abstract:UNLABELLED:The aim of the present study was to investigate hypoxia-inducible factor 1α (HIF-1α) and glucose transporter-1 (GLUT-1) expressions as predictors of response and survival after chemoradiotherapy in pretreatment biopsy specimens from patients with rectal cancer. MATERIALS AND METHODS:The immunohistological e...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2011-05-01 00:00:00
abstract:BACKGROUND/AIM:To develop a tool for estimating the risk of developing new cerebral lesions in 69 melanoma patients receiving radiosurgery for 1-3 cerebral metastases. PATIENTS AND METHODS:Ten factors were investigated: lactate dehydrogenase (LDH), radiosurgery dose, age, gender, performance status, maximum diameter, ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-10-01 00:00:00
abstract:AIM:Mitotane is used in adrenal cancer as adjuvant therapy, monotherapy or combined with other cytotoxic agents in advanced disease, but only 30% of patients respond. The aim of this study was to define the structural requirements for drug activity and to develop analogs with improved adrenalytic action. MATERIALS AND...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-07-01 00:00:00
abstract:BACKGROUND:To distinguish cholangiocarcinoma from inflammatory disease remains difficult when stricture is present in the bile duct. Endoscopic brushing cytology is a convenient method for stricture in the bile duct, however, the diagnostic sensitivity of this method for malignancy is reported to be low (<60%). Msh hom...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2011-03-01 00:00:00
abstract:BACKGROUND/AIM:This study aimed to assess whether low-molecular-weight heparin (LMWH) is effective and safe in preventing postoperative venous thromboembolism (VTE) in patients undergoing esophageal cancer surgery. PATIENTS AND METHODS:In this single-institution, prospective, randomized trial, 73 patients with esophag...
journal_title:Anticancer research
pub_type: 杂志文章,随机对照试验
doi:10.21873/anticanres.13385
更新日期:2019-05-01 00:00:00
abstract::Squamous cell carcinomas (SCC) make up 96% of all oral cancers. Most laboratory SCC studies grow cells as a monolayer, which does not accurately represent the disease in vivo. We used a more relevant multicellular spheroid (MCS) model to study this disease. The SCC9β6KDFyn cell line, which expresses full-length β6 and...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-12-01 00:00:00
abstract::Bone metastases are a sign of advanced tumor disease. Surgical therapy is only occasionally curative. Therefore, the therapeutic goals are a limited surgical excision, immediate mobilization, effective stabilization and the avoidance of local recurrence. We investigated the effect of the anthracycline daunorubicin (DN...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
abstract:BACKGROUND:Melanoma is among the top three cancers to present with brain metastasis. The risk of brain metastases in advanced melanoma increases with disease duration. Cytotoxic chemotherapy does not have a significant role in the management of melanoma patients with brain metastases, neither alone nor in conjunction w...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11038
更新日期:2016-09-01 00:00:00
abstract:BACKGROUND/AIM:Elderly patients represent an important subgroup of patients with brain metastases. A survival score has been developed specifically for these patients. PATIENTS AND METHODS:A total of 544 elderly patients (aged ≥65 years) receiving whole-brain radiotherapy alone were divided into a test (n=272) and a v...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-05-01 00:00:00
abstract:BACKGROUND/AIM:Protein-bound polysaccharide-K (PSK) is extracted from Coriolus versicolor (CM101) and is clinically used in combination therapy for gastrointestinal cancer and small-cell lung carcinoma. We have previously demonstrated that PSK induces apoptosis and inhibites proliferation of promyelomonocytic leukemia ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-07-01 00:00:00
abstract:BACKGROUND:It has recently been reported that sulindac has an apoptotic effect on KYN-2 cells, an undifferentiated hepatoma cell line. The present work investigates whether sulindac also has an apoptotic effect on well-differentiated hepatoma cells and what its potential mechanism might be. MATERIALS AND METHODS:Hep G...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract:AIM:To evaluate (18)F-fluorodihydroxyphenylalanine-positron emission tomography/ contrast-enhanced computed tomography ((18)F-DOPA PET/CT) for the detection of paragangliomas (PARA) without any patient selection, such as genetic predisposition for the development of these tumors, history of metastatic PARA or hormonal ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2016-08-01 00:00:00
abstract:BACKGROUND/AIM:Accumulating evidence shows that various types of cancers induce a specific immune response resulting in the production of antibodies against self-components (autoantibodies). The aim of the present study was to identify antigens for autoantibodies in sera from patients with ovarian cancer, especially cl...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-02-01 00:00:00
abstract::We investigated the in vitro effects of heparin on the growth and interaction of SW480 colon adenocarcinoma cells with the extracellular matrix proteins laminin, fibronectin and collagen IV. Cell adhesion assays were performed in wells precoated with the proteins mentioned. Tumor cell migration and invasiveness were a...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1995-07-01 00:00:00
abstract::Previous studies have demonstrated that berberine decreases N-acetyltransferase (NAT) activity in human leukemia HL-60 cells, however, NAT gene expression has not been investigated. In this study, berberine was selected for testing the inhibition of N-acetylation of 2-aminofluorene (AF) and NAT gene expression in huma...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2005-11-01 00:00:00
abstract::The LAI reactivity of a colon organ specific neoantigen (OSN) was recovered from the urine of advanced colon cancer patients. In this study three physico-chemical steps were employed; precipitation by 80% ammonium sulfate, ion exchange, and molecular sieve chromatography. Each isolate was tested for activity and speci...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1986-07-01 00:00:00
abstract:BACKGROUND:Deregulated RAS/RAF/MAPK and PI3K/AKT/mTOR signaling pathways are found in hepatocellular carcinoma (HCC). This study aimed to test the inhibitory effects of PI-103 (a small molecule inhibitor of PI3K and mTOR) and sorafenib as single agents and in combination on HCC tumorigenesis in an in vivo xenograft mod...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-07-01 00:00:00
abstract:BACKGROUND:While procarbazine with 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea (PC) added to vincristine (PCV) was proven beneficial in the treatment of co-deleted anaplastic oligodendroglioma (AO), the question of whether PC alone is sufficient is important, as vincristine adds toxicity with uncertain benefit. This r...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-10-01 00:00:00
abstract:BACKGROUND:Inhibition of the vascular endothelial growth factor receptor (VEGFR) has been shown to improve the radiation response of several experimental tumors. The present experimental study compares the effect of neoadjuvant, concomitant and adjuvant treatment with PTK787/ZK222584, a specific inhibitor of the VEGFR,...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-09-01 00:00:00
abstract::Brain tumors were induced in inbred Fischer rats (F344) by administration of methylnitrosourea in the drinking water. One of the induced tumors, a pleomorphic glioma (78-219), was established in vitro and propagated as 78FR-G-219 permanent cell line. The tumorigenicity of the established line was investigated by intra...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1981-01-01 00:00:00
abstract::Sirolimus, and its antiproliferative capacity, was studied in vivo in three different syngenic rat tumours in the liver. Sirolimus is an inhibitor of the cytosolic mTOR-kinase, associated with the phosphoinositide-3-kinase/Akt pathway. After one week of daily sirolimus treatment, initiated on the day of tumour-cell in...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2005-03-01 00:00:00
abstract:BACKGROUND/AIM:Typical carcinoids (TC) and atypical carcinoids (AC) are rare diseases. A paucity of randomized studies and disagreements among various guidelines makes the management challenging. PATIENTS AND METHODS:Using codes for TC (8240) and AC (8249) in the National Cancer Database (NCDB), all surgically resecte...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13900
更新日期:2019-12-01 00:00:00
abstract::NMR spectroscopy was used to analyse perchloric acid extracts of normal human brain, murine brain cell cultures, glioblastoma tissue and the glioblastoma cell line U-87. 1H NMR spectra revealed the presence of elevated levels of UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine in glioblastoma extracts and the gli...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1994-05-01 00:00:00
abstract:PURPOSE:The aim of this study was to determine the relationship between survival and value of molecular markers in the primary tumour in a group of patients with irresectable rectal cancer, treated with preoperative chemo-radiotherapy. MATERIALS AND METHODS:Immunohistochemistry for p53, p21, bcl-2 and Ki-67 was perfor...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2004-03-01 00:00:00
abstract::The recent meeting of the AGMIF (Working Group for Mistletoe Therapy and Immunological Research) held in Herdecke, Germany, on October, 3rd, 1997, covered recent developments in the field of immunological and biological properties of Viscum album L., the European mistletoe, which is used for adjuvant cancer treatment....
journal_title:Anticancer research
pub_type:
doi:
更新日期:1998-09-01 00:00:00
abstract:BACKGROUND/AIM:In addition to its cytocidal effects as a microtubule dynamics inhibitor, eribulin mesylate (eribulin) regulates the tumour microenvironment. We examined the clinical significance of tumour infiltrating lymphocytes (TILs) and transforming growth factor-β (TGF-β), which are local markers of host immunity,...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.14317
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND:The BCL2 proto-oncogene in non-Hodgkin's lymphoma is a dominant inhibitor of apoptosis. The goal of this work was to develop a (177)Lu-labeled anti-BCL2-peptide nucleic acid (PNA) conjugate designed for dual modality NHL therapy, i.e., simultaneous down-regulation of BCL2-mediated resistance to apoptosis and...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2011-10-01 00:00:00
abstract:AIM:We analyzed pentafecta outcomes [complication-free, continence, potency, negative surgical margins (NSM)], biochemical recurrence (BCR)-free] of 230 patients undergoing robotic-assisted radical prostatectomy (RARP) with bilateral neurovascular (NVB) preservation. PATIENTS AND METHODS:Patient outcomes (group I, cas...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-09-01 00:00:00