An octamer element is required for the expression of the alpha H2B histone gene during the early development of the sea urchin.


:Early (alpha) histone genes are one of several histone gene families in the sea urchin genome. They are expressed at high levels in blastula-stage embryos and are inactivated by the early gastrula stage. By microinjecting mutant early H2B genes into sea urchin zygotes and monitoring their transcriptional activity in blastula- and gastrula-stage embryos, we sought to identify the cis-regulatory elements responsible for this dramatic change in early H2B gene activity. We found that deletion of DNA 5' of -71 and 3' of +591 did not affect the timing or magnitude of early H2B gene expression. Neither was early H2B gene expression affected by the replacement of sequences downstream of -36 with the corresponding region of the L1 late H2B gene, expressed after the peak transcription of the early H2B gene. Further deletion of early H2B promoter sequences from -71 to -56, removing a conserved octamer element, resulted in near-complete inactivation of the early H2B gene in both blastula- and gastrula-stage embryos. Also inactivating early H2B gene expression were an internal deletion of the octamer element and a base substitution mutation that altered its sequence. This base substitution mutation also caused a parallel reduction in the ability of the octamer element to bind a factor present in nuclear extracts of sea urchin blastulae. These data strongly suggest that the proper expression of the early H2B gene in cleavage- and blastula-stage embryos depends on the octamer element and a factor with which it interacts.


Dev Biol


Developmental biology


Bell J,Char BR,Maxson R




Has Abstract


1992-04-01 00:00:00














  • Caenorhabditis elegans cog-1 locus encodes GTX/Nkx6.1 homeodomain proteins and regulates multiple aspects of reproductive system development.

    abstract::The development of the reproductive system in Caenorhabditis elegans is a well-established model system for patterning and organogenesis. We report the characterization of the cog-1 gene, mutations in which cause novel phenotypes in late patterning in vulval lineages, establishment of the vulva-uterine connection, dev...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Palmer RE,Inoue T,Sherwood DR,Jiang LI,Sternberg PW

    更新日期:2002-12-15 00:00:00

  • Nerve and muscle development in paralysé mutant mice.

    abstract::Nerve and muscle development was studied in paralysé mutant mice. The mutant phenotype is first recognizable 6-7 days after birth (PN 6-PN 7) as cessation of muscle growth and weakness and incoordination of movement. Mutant animals die between 2 and 3 weeks of age. Muscle fibers from paralysé mutants had a unimodal di...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Blondet B,Duxson MJ,Harris AJ,Melki J,Guénet JL,Pinçon-Raymond M,Rieger F

    更新日期:1989-03-01 00:00:00

  • FGF-stimulated outgrowth and proliferation of limb mesoderm is dependent on syndecan-3.

    abstract::The outgrowth of the mesoderm of the developing limb bud in response to the apical ectodermal ridge (AER) is mediated at least in part by members of the FGF family. Recent studies have indicated that FGFs need to interact with heparan sulfate proteoglycans in order to bind to and activate their specific cell surface r...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Dealy CN,Seghatoleslami MR,Ferrari D,Kosher RA

    更新日期:1997-04-15 00:00:00

  • Caveolin-1 regulates dorsoventral patterning through direct interaction with beta-catenin in zebrafish.

    abstract::Caveolin-1 (Cav-1) is the principal component of plasma membrane caveolae that negatively regulates a number of cellular signaling events including canonical Wnt signaling. Activation of the Wnt/beta-catenin pathway is essential for dorsal organizer formation and specification in early vertebrate embryos, but it remai...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Mo S,Wang L,Li Q,Li J,Li Y,Thannickal VJ,Cui Z

    更新日期:2010-08-01 00:00:00

  • An organizing role for the TGF-β signaling pathway in axes formation of the annelid Capitella teleta.

    abstract::Embryonic organizers are signaling centers that coordinate developmental events within an embryo. Localized to either an individual cell or group of cells, embryonic organizing activity induces the specification of other cells in the embryo and can influence formation of body axes. In the spiralian Capitella teleta, p...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Lanza AR,Seaver EC

    更新日期:2018-03-01 00:00:00

  • Analysis of early human neural crest development.

    abstract::The outstanding migration and differentiation capacities of neural crest cells (NCCs) have fascinated scientists since Wilhelm His described this cell population in 1868. Today, after intense research using vertebrate model organisms, we have gained considerable knowledge regarding the origin, migration and differenti...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Betters E,Liu Y,Kjaeldgaard A,Sundström E,García-Castro MI

    更新日期:2010-08-15 00:00:00

  • The Arabidopsis TFIID factor AtTAF6 controls pollen tube growth.

    abstract::Initiation of transcription mediated by RNA polymerase II requires a number of transcription factors among which TFIID is the major core promoter recognition factor. TFIID is composed of highly conserved factors which include the TATA-binding protein (TBP) and about 14 TBP-associated factors (TAFs). Recently, the comp...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Lago C,Clerici E,Dreni L,Horlow C,Caporali E,Colombo L,Kater MM

    更新日期:2005-09-01 00:00:00

  • Regulation of locomotion and motoneuron trajectory selection and targeting by the Drosophila homolog of Olig family transcription factors.

    abstract::During the development of locomotion circuits it is essential that motoneurons with distinct subtype identities select the correct trajectories and target muscles. In vertebrates, the generation of motoneurons and myelinating glia depends on Olig2, one of the five Olig family bHLH transcription factors. We investigate...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Oyallon J,Apitz H,Miguel-Aliaga I,Timofeev K,Ferreira L,Salecker I

    更新日期:2012-09-15 00:00:00

  • Hepatic Notch2 deficiency leads to bile duct agenesis perinatally and secondary bile duct formation after weaning.

    abstract:UNLABELLED:Notch signaling plays an acknowledged role in bile-duct development, but its involvement in cholangiocyte-fate determination remains incompletely understood. We investigated the effects of early Notch2 deletion in Notch2(fl/fl)/Alfp-Cre(tg/-) ("Notch2-cKO") and Notch2(fl/fl)/Alfp-Cre(-/-) ("control") mice. F...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Falix FA,Weeda VB,Labruyere WT,Poncy A,de Waart DR,Hakvoort TB,Lemaigre F,Gaemers IC,Aronson DC,Lamers WH

    更新日期:2014-12-15 00:00:00

  • Studies on the differentiation of egg envelopes. I. The starfish, Astropecten aurantiacus.

    abstract::We have studied the differentiation of the oocyte vitelline coat (VC) and jelly coat (JC) of the starfish, Astropecten aurantiacus. The precursor material of both envelopes is secreted by the oocyte while the follicle cells do not appear to participate in the secretory process. The first indication of differentiation ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Santella L,Monroy A,Rosati F

    更新日期:1983-10-01 00:00:00

  • Expression of activin transcripts in follicle cells and oocytes of Xenopus laevis.

    abstract::Activin is a potent inducer of axial mesoderm in vitro and may have a similar role in vivo. Xenopus laevis eggs contain significant amounts of activin or activin-like factors, but maternal activin transcripts have not been detected in Xenopus eggs. The maternal activin protein might be translated from activin beta A o...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Rebagliati MR,Dawid IB

    更新日期:1993-10-01 00:00:00

  • Sgn1, a basic helix-loop-helix transcription factor delineates the salivary gland duct cell lineage in mice.

    abstract::The salivary system in mammals is comprised of three independently developed pairs of organs, the parotid, submaxillar, and sublingual glands. Each gland is composed of various ductal and acinar cell types that fulfill multiple roles. However, the molecular mechanisms regulating their biogenesis and functions are stil...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Yoshida S,Ohbo K,Takakura A,Takebayashi H,Okada T,Abe K,Nabeshima Y

    更新日期:2001-12-15 00:00:00

  • Sequential programs of retinoic acid synthesis in the myocardial and epicardial layers of the developing avian heart.

    abstract::Endogenous patterns of retinoic acid (RA) signaling in avian cardiac morphogenesis were characterized by localized expression of a key RA-synthetic enzyme, RALDH2, which displayed a biphasic pattern during heart development. RALDH2 immunoreactivity was initially apparent posterior to Hensen's node of stage 5-6 embryos...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Xavier-Neto J,Shapiro MD,Houghton L,Rosenthal N

    更新日期:2000-03-01 00:00:00

  • C. elegans patched-3 is an essential gene implicated in osmoregulation and requiring an intact permease transporter domain.

    abstract::The nematode Caenorhabditis elegans has retained a rudimentary Hedgehog (Hh) signalling pathway; Hh and Smoothened (Smo) homologs are absent, but two highly related Patched gene homologs, ptc-1 and ptc-3, and 24 ptc-related (ptr) genes are present. We previously showed that ptc-1 is essential for germ line cytokinesis...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Soloviev A,Gallagher J,Marnef A,Kuwabara PE

    更新日期:2011-03-15 00:00:00

  • Live cell imaging of cell movement and transdifferentiation during regeneration of an amputated multicellular body of the social amoeba Dictyostelium discoideum.

    abstract::The regeneration of lost body parts is a fascinating phenomenon exhibited by some multicellular organisms. In social amoebae, such as Dictyostelium discoideum, the pseudoplasmodium is a temporary migratory multicellular structure with high regeneration ability. It consists of future stalk cells (prestalk cells) at the...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Mohri K,Tanaka R,Nagano S

    更新日期:2020-01-01 00:00:00

  • Saunders's framework for understanding limb development as a platform for investigating limb evolution.

    abstract::John W. Saunders, Jr. made seminal discoveries unveiling how chick embryos develop their limbs. He discovered the apical ectodermal ridge (AER), the zone of polarizing activity (ZPA), and the domains of interdigital cell death within the developing limb and determined their function through experimental analysis. Thes...

    journal_title:Developmental biology

    pub_type: 杂志文章,评审


    authors: Young JJ,Tabin CJ

    更新日期:2017-09-15 00:00:00

  • Opioid receptor-induced GTPgamma35S binding during mouse development.

    abstract::Although a large superfamily of G-protein-coupled receptors serves multiple functions, little is known about their functional activation during ontogeny. To examine the functional activation of the mu-opioid receptor (MOR) and the delta-opioid receptor (DOR) during development, sections of mouse embryos and fetuses fr...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Nitsche JF,Pintar JE

    更新日期:2003-01-01 00:00:00

  • Developmental clustering of ion channels at and near the node of Ranvier.

    abstract::Voltage-gated Na(+) and K(+) channels are localized to distinct subcellular domains in mammalian myelinated nerve fibers. Specifically, Na(+) channels are clustered in high densities at nodes of Ranvier, while K(+) channels are found in juxtaparanodal zones just beyond regions of axoglial contact where sequential laye...

    journal_title:Developmental biology

    pub_type: 杂志文章,评审


    authors: Rasband MN,Trimmer JS

    更新日期:2001-08-01 00:00:00

  • Changes in the catalytic properties of DNA ligases during early sea urchin development.

    abstract::Two distinct DNA ligases are expressed during early sea urchin embryogenesis. A light form (50 kDa) is found in unfertilized eggs (oocyte form) and a heavier enzyme (110 kDa) is observed at the two-cell stage (embryonic form). The chronology of the change reveals that the embryonic form is detected 90 min after fertil...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Prigent C,Maniey D,Lefresne J,Epel D,Signoret J,David JC

    更新日期:1987-11-01 00:00:00

  • Syd/JIP3 controls tissue size by regulating Diap1 protein turnover downstream of Yorkie/YAP.

    abstract::How organisms control organ size is not fully understood. We found that Syd/JIP3 is required for proper wing size in Drosophila. JIP3 mutations are associated with organ size defects in mammals. The underlying mechanisms are not well understood. We discovered that Syd/JIP3 inhibition results in a downregulation of the...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Ahmad V,Vadla GP,Chabu CY

    更新日期:2021-01-01 00:00:00

  • A growth-promoting influence from the mesonephros during limb outgrowth.

    abstract::It has been suggested that the mesonephros has a role in normal limb development. This hypothesis was directly tested by removing the mesonephros adjacent to the presumptive limb region of stage 12-18 chick embryos using microsurgery or laser ablation. The experimental manipulation resulted in reduced limb outgrowth o...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Geduspan JS,Solursh M

    更新日期:1992-05-01 00:00:00

  • Cell lineage conversion in the sea urchin embryo.

    abstract::The mesoderm of the sea urchin embryo conventionally is divided into two populations of cells; the primary mesenchyme cells (PMCs), which produce the larval skeleton, and the secondary mesenchyme cells (SMCs), which differentiate into a variety of cell types but do not participate in skeletogenesis. In this study we e...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Ettensohn CA,McClay DR

    更新日期:1988-02-01 00:00:00

  • Spectrin in mouse gametogenesis and embryogenesis.

    abstract::Antibodies to nonerythroid alpha spectrin (p 230) were used to study the distribution of this polypeptide in mouse germ cells, zygote, and early embryonic cells. In the primordial germ cells, fetal oocytes, and spermatogonia, spectrin was found predominantly in the form of a narrow condensed subplasmalemmal band, as i...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Damjanov I,Damjanov A,Lehto VP,Virtanen I

    更新日期:1986-03-01 00:00:00

  • The N-BAR domain protein, Bin3, regulates Rac1- and Cdc42-dependent processes in myogenesis.

    abstract::Actin dynamics are necessary at multiple steps in the formation of multinucleated muscle cells. BAR domain proteins can regulate actin dynamics in several cell types, but have been little studied in skeletal muscle. Here, we identify novel functions for the N-BAR domain protein, Bridging integrator 3 (Bin3), during my...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Simionescu-Bankston A,Leoni G,Wang Y,Pham PP,Ramalingam A,DuHadaway JB,Faundez V,Nusrat A,Prendergast GC,Pavlath GK

    更新日期:2013-10-01 00:00:00

  • Mef2c factors are required for early but not late addition of cardiomyocytes to the ventricle.

    abstract::During heart formation, the heart grows and undergoes dramatic morphogenesis to achieve efficient embryonic function. Both in fish and amniotes, much of the growth occurring after initial heart tube formation arises from second heart field (SHF)-derived progenitor cell addition to the arterial pole, allowing chamber f...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Kula-Alwar D,Marber MS,Hughes SM,Hinits Y

    更新日期:2021-02-01 00:00:00

  • spe-43 is required for sperm activation in C. elegans.

    abstract::Successful fertilization requires that sperm are activated prior to contacting an oocyte. In C. elegans, this activation process, called spermiogenesis, transforms round immobile spermatids into motile, fertilization-competent spermatozoa. We describe the phenotypic and genetic characterization of spe-43, a new compon...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Krauchunas AR,Mendez E,Ni JZ,Druzhinina M,Mulia A,Parry J,Gu SG,Stanfield GM,Singson A

    更新日期:2018-04-15 00:00:00

  • Analysis of visual system development in Drosophila melanogaster: mutations at the Glued locus.

    abstract::We have analyzed several aspects of the development of flies carrying mutations at the Glued locus. Optic lobe abnormalities in individuals heterozygous for the original Glued allele were previously shown to result from an action of this mutation in the retinula cells. We have estimated when the functioning of this ge...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Harte PJ,Kankel DR

    更新日期:1983-09-01 00:00:00

  • Mesendoderm extension and mantle closure in Xenopus laevis gastrulation: combined roles for integrin alpha(5)beta(1), fibronectin, and tissue geometry.

    abstract::We describe mesendoderm morphogenesis during gastrulation in the frog Xenopus laevis and investigate the mechanics of these movements with tissue explants. When a dorsal marginal zone explant is plated onto fibronectin, the mesendoderm moves away from the dorsal axial tissues as an intact sheet. Mesendodermal cells wi...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Davidson LA,Hoffstrom BG,Keller R,DeSimone DW

    更新日期:2002-02-15 00:00:00

  • Inductive events in the patterning of the Xenopus laevis hatching and cement glands, two cell types which delimit head boundaries.

    abstract::In Xenopus, the hatching and cement glands form at important boundaries in the head. We have examined induction of these glands in order to see the basis for this ectodermal patterning. Ectoderm can be induced to form hatching and cement glands by dorsal mesoderm and by the neural plate. Because the neural plate is su...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Drysdale TA,Elinson RP

    更新日期:1993-07-01 00:00:00

  • The anterior visceral endoderm of the mouse embryo is established from both preimplantation precursor cells and by de novo gene expression after implantation.

    abstract::Initiation of the development of the anterior-posterior axis in the mouse embryo has been thought to take place only when the anterior visceral endoderm (AVE) emerges and starts its asymmetric migration. However, expression of Lefty1, a marker of the AVE, was recently found to initiate before embryo implantation. This...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Torres-Padilla ME,Richardson L,Kolasinska P,Meilhac SM,Luetke-Eversloh MV,Zernicka-Goetz M

    更新日期:2007-09-01 00:00:00