Abstract:
BACKGROUND:Cigarette smoke (CS) is the main cause in the development of chronic obstructive pulmonary disease (COPD), the pathogenesis of which is related to an extended inflammatory response. In this study, we investigated the effect of low and high doses of gas phase cigarette smoke (GPS) on cultured lymphocyte progenitor cells, using techniques to assess cell viability and to elucidate whether cells die of apoptosis or necrosis upon exposure to different doses of GPS. METHODS:In our approach we utilised a newly-established system of exposure of cells to GPS that is highly controlled, accurately reproducible and simulates CS dosage and kinetics that take place in the smokers' lung. This system was used to study the mode of cell death upon exposure to GPS in conjunction with a range of techniques widely used for cell death studies such as Annexin V staining, activation of caspase -3, cytoplasmic release of cytochrome C, loss of mitochondrial membrane potential and DNA fragmentation. RESULTS:Low doses of GPS induced specific apoptotic indexes in CCRF-CEM cells. Specifically, cytochrome C release and cleaved caspase-3 were detected by immunofluorescence, upon treatment with 1-3 puffs GPS. At 4 h post-exposure, caspase-3 activation was observed in western blot analysis, showing a decreasing pattern as GPS doses increased. Concomitant with this behaviour, a dose-dependent change in Delta psi m depolarization was monitored by flow cytometry 2 h post-exposure, while at 4 h Delta psi m collapse was observed at the higher doses, indicative of a shift to a necrotic demise. A reduction in DNA fragmentation events produced by 5 puffs GPS as compared to those provoked by 3 puffs GPS, also pointed towards a necrotic response at the higher dose of GPS. CONCLUSION:Collectively, our results support that at low doses gas phase cigarette smoke induces apoptosis in cultured T-lymphocytes, whereas at high doses GPS leads to necrotic death, by-passing the characteristic stage of caspase-3 activation and, thus, the apoptotic route.
journal_name
Respir Resjournal_title
Respiratory researchauthors
Sdralia ND,Patmanidi AL,Velentzas AD,Margaritis LH,Baltatzis GE,Hatzinikolaou DG,Stavridou Adoi
10.1186/1465-9921-10-82subject
Has Abstractpub_date
2009-09-10 00:00:00pages
82eissn
1465-9921issn
1465-993Xpii
1465-9921-10-82journal_volume
10pub_type
杂志文章abstract:BACKGROUND:During a lipopolysaccharide-induced lung inflammation, a massive accumulation of neutrophils occurs, which is normally cleared by macrophage phagocytosis following neutrophil apoptosis. However, in cases of extensive apoptosis the normal clearance system may fail, resulting in extensive neutrophil secondary ...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-7-143
更新日期:2006-12-14 00:00:00
abstract:BACKGROUND:Chronic respiratory diseases (CRDs) are leading causes of morbidity worldwide. However, the spatial and temporal trends in prevalence and incidence of CRDs have not been estimated. METHODS:Based on data from the Global Burden of Diseases, Injuries, and Risk Factors Study 2017, we analyzed the prevalence and...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-020-1291-8
更新日期:2020-02-11 00:00:00
abstract:BACKGROUND:The receptor for advanced glycation end products (RAGE) is a multiligand signal transduction receptor that can initiate and perpetuate inflammation. Its soluble isoform (sRAGE) acts as a decoy receptor for RAGE ligands, and is thought to afford protection against inflammation. With the present study, we aime...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-12-37
更新日期:2011-03-30 00:00:00
abstract:BACKGROUND:An imbalance in the generation of pro-inflammatory leukotrienes, and counter-regulatory lipoxins is present in severe asthma. We measured leukotriene B4 (LTB4), and lipoxin A4 (LXA4) production by alveolar macrophages (AMs) and studied the impact of corticosteroids. METHODS:AMs obtained by fiberoptic bronch...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-11-71
更新日期:2010-06-07 00:00:00
abstract:BACKGROUND:Allergen-containing subpollen particles (SPP) are released from whole plant pollen upon contact with water or even high humidity. Because of their size SPP can preferentially reach the lower airways where they come into contact with surfactant protein (SP)-D. Our previous work demonstrated that SP-D increase...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-11-83
更新日期:2010-06-22 00:00:00
abstract:BACKGROUND:25-hydroxycholesterol (25-HC) is one of the oxysterols, which are oxidized derivatives of cholesterol, and has been reported to be involved in the pathogenesis of atherosclerosis and Alzheimer's disease. In lung, the possible involvement of 25-HC in airway diseases has been revealed. In the present study, we...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-13-63
更新日期:2012-07-31 00:00:00
abstract:BACKGROUND:Recent consensus statements demonstrate the breadth of the chronic rhinosinusitis (CRS) differential diagnosis. However, the classification and mechanisms of different CRS phenotypes remains problematic. METHOD:Statistical patterns of subjective and objective findings were assessed by retrospective chart re...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-6-149
更新日期:2005-12-19 00:00:00
abstract:BACKGROUND:Fibrocytes are implicated in Idiopathic Pulmonary Fibrosis (IPF) pathogenesis and increased proportions in the circulation are associated with poor prognosis. Upon tissue injury, fibrocytes migrate to the affected organ. In IPF patients, circulating fibrocytes are increased especially during exacerbations, h...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-018-0798-8
更新日期:2018-05-10 00:00:00
abstract::Transposable elements (TEs) are a class of mobile genetic elements (MGEs) that were long regarded as junk DNA, which make up approximately 45% of the genome. Although most of these elements are rendered inactive by mutations and other gene silencing mechanisms, TEs such as long interspersed nuclear elements (LINEs) ar...
journal_title:Respiratory research
pub_type: 杂志文章,评审
doi:10.1186/1465-9921-14-99
更新日期:2013-10-05 00:00:00
abstract:BACKGROUND:Current therapy for relieving bronchoconstriction may be ineffective in severe asthma, particularly in the small airways. The aim of this study was to further characterise responses to the recently identified novel bronchodilators rosiglitazone (RGZ) and chloroquine (CQ) under conditions where β-adrenoceptor...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-15-29
更新日期:2014-03-12 00:00:00
abstract:BACKGROUND:CXCR4 is the receptor for chemokine CXCL12 and reportedly plays an important role in systemic vascular repair and remodeling, but the role of CXCR4 in development of pulmonary hypertension and vascular remodeling has not been fully understood. METHODS:In this study we investigated the role of CXCR4 in the d...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-12-21
更新日期:2011-02-04 00:00:00
abstract:BACKGROUND:WNT/β-catenin signaling plays an important role in the pathogenesis of idiopathic pulmonary fibrosis (IPF); however, the role of WNT10A via transforming growth factor (TGF)-β signaling remains unclear. METHODS:We evaluated the expression of WNT10A and TGF-β in bleomycin (BLM)-treated mice and the interactio...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-016-0357-0
更新日期:2016-04-12 00:00:00
abstract:BACKGROUND:It is generally accepted that emphysematous lungs are characterized by an increase in the numbers of neutrophils, macrophages, and CD8+ T lymphocytes, the lasts having increased cytotoxic activity. Because systemic inflammation is also a component of emphysema, we hypothesize that peripheral CD8+ T lymphocyt...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-8-62
更新日期:2007-09-06 00:00:00
abstract:BACKGROUND:Proteomic studies of respiratory disorders have the potential to identify protein biomarkers for diagnosis and disease monitoring. Utilisation of sensitive quantitative proteomic methods creates opportunities to determine individual patient proteomes. The aim of the current study was to determine if quantita...
journal_title:Respiratory research
pub_type: 杂志文章,随机对照试验
doi:10.1186/1465-9921-12-124
更新日期:2011-09-22 00:00:00
abstract:BACKGROUND:Idiopathic pulmonary fibrosis (IPF) is a chronically progressive interstitial lung disease of unknown etiology. Previously, we have demonstrated the selective upregulation of the macrophage-derived chemokine CCL22 and the thymus activation-regulated chemokine CCL17 among chemokines, in a rat model of radiati...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-10-80
更新日期:2009-08-29 00:00:00
abstract:BACKGROUND:Colonization of the airways with potential pathogenic bacteria is observed in a number of chronic respiratory diseases, such as COPD or cystic fibrosis. Infections with respiratory viruses are known triggers of exacerbations of these diseases. We here investigated if pre-exposure to bacteria alters the respo...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-016-0382-z
更新日期:2016-06-03 00:00:00
abstract::The novel coronavirus disease (COVID-19) outbreak started in December 2019 in Wuhan, China, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The CT image is used to assess the disease progress, whereas the continued two times of negative results from SARS-CoV-2 nucleic acid detection had been co...
journal_title:Respiratory research
pub_type: 信件
doi:10.1186/s12931-020-01363-7
更新日期:2020-04-22 00:00:00
abstract:BACKGROUND:Two randomised, double-blind, placebo-controlled trials have investigated the efficacy of IV alpha-1 antitrypsin (AAT) augmentation therapy on emphysema progression using CT densitometry. METHODS:Data from these similar trials, a 2-center Danish-Dutch study (n = 54) and the 3-center EXAcerbations and CT sca...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-11-136
更新日期:2010-10-05 00:00:00
abstract::The transcription factors nuclear factor interleukin-6 (NF-IL6), early growth response-1 (EGR-1) and hypoxia-inducible factor-1 (HIF-1) have important roles in the molecular pathophysiology of hypoxia-associated pulmonary disease. NF-IL6 controls the production of interleukin (IL)-6 in vascular endothelial cells, whic...
journal_title:Respiratory research
pub_type: 杂志文章,评审
doi:10.1186/rr27
更新日期:2000-01-01 00:00:00
abstract::By airway surface liquid, we mean a thin fluid continuum consisting of the airway lining layer and the alveolar lining layer, which not only serves as a protective barrier against foreign particles but also contributes to maintaining normal respiratory mechanics. In recent years, measurements of the rheological proper...
journal_title:Respiratory research
pub_type: 杂志文章,评审
doi:10.1186/s12931-019-1229-1
更新日期:2019-12-04 00:00:00
abstract:BACKGROUND:The identification of smoking-related lung disease in current and former smokers with normal FEV1 is complex, leading to debate regarding using a ratio of forced expiratory volume in 1 s to forced vital capacity (FEV1/FVC) of less than 0.70 versus the predicted lower limit of normal (LLN) for diagnosis of ai...
journal_title:Respiratory research
pub_type: 杂志文章,多中心研究
doi:10.1186/s12931-018-0911-z
更新日期:2018-11-20 00:00:00
abstract:BACKGROUND:Recent studies have indicated the presence of multipotent mesenchymal stromal cells (MSCs) in human lung diseases. Excess airway smooth muscle, myofibroblasts and activated fibroblasts have each been noted in asthma, suggesting that mesenchymal progenitor cells play a role in asthma pathogenesis. We therefor...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-11-127
更新日期:2010-09-21 00:00:00
abstract:BACKGROUND:Idiopathic pulmonary fibrosis (IPF) is a progressive, fatal disorder with a variable disease course. The recent advancement of antifibrotic therapy has increased the need for reliable and specific biomarkers. This study aimed to assess alveolar epithelial biomarkers as predictors for the efficacy of the anti...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-020-01582-y
更新日期:2020-11-30 00:00:00
abstract:BACKGROUND:Pulmonary arterial hypertension (PAH) is associated with inflammatory response but it is unknown whether it is associated with alterations in NNMT activity and MNA plasma concentration. Here we examined changes in NNMT-MNA pathway in PAH in rats and humans. METHODS:PAH in rats was induced by a single subcut...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-016-0423-7
更新日期:2016-08-31 00:00:00
abstract:BACKGROUND:Allergic asthma is characterized by airway inflammation in response to antigen exposure, leading to airway remodeling and lung dysfunction. Epithelial-mesenchymal transition (EMT) may play a role in airway remodeling through the acquisition of a mesenchymal phenotype in airway epithelial cells. TGF-β1 is kno...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-14-118
更新日期:2013-11-01 00:00:00
abstract:BACKGROUND:The investigation of mesenchymal stromal cell (MSC)-conditioned medium or extracellular vesicles (exosomes or microvesicles) as a remedy for acute lung injury (ALI) or acute respiratory distress syndrome (ARDS) has become a fast-growing field in recent years. Our purpose was to conduct a meta-analysis to inv...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-020-01574-y
更新日期:2020-11-20 00:00:00
abstract:BACKGROUND:Early life impairments leading to lower lung function by adulthood are considered as risk factors for chronic obstructive pulmonary disease (COPD). Recently, we compared the lung transcriptomic profile between two mouse strains with extreme total lung capacities to identify plausible pulmonary function deter...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-018-0795-y
更新日期:2018-06-06 00:00:00
abstract:BACKGROUND:Organ regeneration in mammals is hypothesized to require a functional pool of stem or progenitor cells, but the role of these cells in lung regeneration is unknown. METHODS:Based on the fact that postnatal regeneration of alveolar tissue has been attributed to alveolar epithelial cells, we established a hem...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-016-0480-y
更新日期:2016-12-07 00:00:00
abstract:BACKGROUND:Exposure to cigarette smoke is considered a major risk factor for the development of lung diseases, since its causative role has been assessed in the induction and maintenance of an inflamed state in the airways. Lung fibroblasts can contribute to these processes, due to their ability to produce proinflammat...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/1465-9921-8-23
更新日期:2007-03-12 00:00:00
abstract:INTRODUCTION:Bacteria have been extensively implicated in the development of smoking related diseases, such as COPD, by either direct infection or bacteria-mediated inflammation. In response to the health risks associated with tobacco exposure, the use of electronic cigarettes (e-cigs) has increased. This study compare...
journal_title:Respiratory research
pub_type: 杂志文章
doi:10.1186/s12931-019-1206-8
更新日期:2019-12-18 00:00:00