Inhibition of human red blood cell glutathione reductase by valproic acid.

abstract：:Naturally occurring amino acid substitutions conferring resistance to methotrexate (MTX) have been reported previously at codon positions 22 (leu-->arg, phe) and 31 (phe-->ser, trp) of mammalian dihydrofolate reductases (DHFR). To explore the character of other substitutions, a polymerase chain reaction (PCR)-assisted...

journal_title：Biochemical pharmacology

authors： Morris JA,McIvor RS

更新日期：1994-03-29 00:00:00

abstract：:Our previous work established that hypocholesterolemic agents altered K-ras intracellular localization in lung. Here, we examined K-ras activity to define further its potential importance in lung carcinogenesis. K-ras activity in lungs from male A/J, Swiss and C57BL/6 mice was examined. For 3 weeks, mice consumed eith...

journal_title：Biochemical pharmacology

authors： Calvert RJ,Tepper S,Kammouni W,Anderson LM,Kritchevsky D

更新日期：2006-12-15 00:00:00

abstract：:Purified ornithine decarboxylase (EC 4.1.1.17, ODC) transamidated with four putrescine moieties on four glutamine residues through the action of transglutaminase (EC 2.3.2.13, TGase) purified from guinea pig liver, when added to isolated rat liver nuclei, stoichiometrically increased the activity of RNA polymerase I (...

journal_title：Biochemical pharmacology

authors： Russell DH,Manen CA

更新日期：1982-11-01 00:00:00

abstract：:5-Hydroxytryptamine (serotonin, 5-HT) stimulates basal adenylyl cyclase activity in membranes from guinea pig or rat hippocampi, but 5-HT inhibits forskolin-stimulated adenylyl cyclase activity in these same membranes. The opposing effects of 5-HT on adenylyl cyclase activity indicate that distinct 5-HT receptors, pos...

journal_title：Biochemical pharmacology

authors： De Vivo M,Maayani S

更新日期：1990-10-01 00:00:00

abstract：:Endomorphin-2 (Tyr-Pro-Phe-PheNH(2)) was discovered as an endogenous ligand for the mu-opioid receptor. The physiological function of endomorphin-2 as a neurotransmitter or neuromodulator may cease through the rapid enzymatic process in the synapse of brain, as for other neuropeptides. The present study was conducted ...

journal_title：Biochemical pharmacology

authors： Sakurada C,Sakurada S,Hayashi T,Katsuyama S,Tan-No K,Sakurada T

更新日期：2003-08-15 00:00:00

abstract：:Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease characterized by synovial membrane hyperplasia, infiltration of inflammatory cells and bone tissue destruction. Although there have been many measures taken for RA therapy in recent years, they are not sufficiently safe or effective. Thus, it is very i...

journal_title：Biochemical pharmacology

authors： Wu Y,Pan W,Hu X,Zhang A,Wei W

更新日期：2021-01-01 00:00:00

abstract：:Acetaminophen (AP) is a widely-used analgesic agent that has been linked to human liver and kidney disease with prolonged or high-dose usage. In rodents, the target organs that are affected include liver, kidney, and the olfactory mucosa. AP toxicity requires cytochrome P450(CYP)-mediated metabolic activation, and the...

journal_title：Biochemical pharmacology

authors： Genter MB,Liang HC,Gu J,Ding X,Negishi M,McKinnon RA,Nebert DW

更新日期：1998-06-01 00:00:00

abstract：:Incubation of human carcinoma cells with mitoxantrone resulted in an intracellular distribution of the drug into cytoplasmic, nuclear and cytoskeletal compartments occurring within 1 min of drug treatment. Incubation of the cells in drug-free medium resulted in an efflux of the drug such that 80% of the intracellular ...

journal_title：Biochemical pharmacology

authors： Roberts RA,Cress AE,Dalton WS

更新日期：1989-12-01 00:00:00

abstract：:The development of pharmacological and biological inhibitors of receptor tyrosine kinases (RTKs) has changed the treatment paradigm of several neoplastic diseases. However, the occurrence of intrinsic and acquired resistance represents a limit to the efficacy of these drugs even in RTK-addicted cancers. The identifica...

journal_title：Biochemical pharmacology

authors： Lanzi C,Cassinelli G

更新日期：2020-08-01 00:00:00

abstract：:Diisopropyl-1,3-dithiol-2-ylidenemalonate (malotilate) was studied for and compared with cyanidanol, hydrocortisone and colchicin on its impact on fibroblast cultures. Under in vitro conditions, malotilate specifically reduces collagen synthesis of fibroblasts. In addition, malotilate is an efficient inhibitor of fibr...

journal_title：Biochemical pharmacology

authors： Poeschl A,Rehn D,Dumont JM,Mueller PK,Hennings G

更新日期：1987-11-15 00:00:00

abstract：:Western blotting and densitometric analysis of extracts obtained from EDTA extraction of skin segments showed greater extracellular Lipocortin 1 (LC1) in skin sites from steroid-treated animals compared to that seen in matched vehicle treated animals. Extracellular LC1 was maximal 3 hr after steroid, less was found in...

journal_title：Biochemical pharmacology

authors： Ahluwalia A,Mohamed RW,Flower RJ

更新日期：1994-10-18 00:00:00

abstract：:We studied the effect of DW2282-,[(S)-(+)-4-phenyl-1-[N-(4-aminobenzoyl)-indoline-5-sulfonyl-4,5-dihydro-2-imidazolone].hydrochloride], a newly developed anti-cancer agent, on cell proliferation, cell cycle progression, and induction of apoptosis in human promyelocytic leukemia (HL-60) cells. DW2282, a diarylsulfonylu...

journal_title：Biochemical pharmacology

authors： Piao W,Yoo J,Lee DK,Hwang HJ,Kim JH

更新日期：2001-12-01 00:00:00

abstract：:The effects of cetaben and clofibric acid were compared on the activities of peroxisomal enzymes in the liver and kidney of male Wistar rats. Cetaben at 200 mg/kg body wt increased the activities of all of the enzymes in the liver that were studied two to eight times, whereas the changes induced by the same dose of cl...

journal_title：Biochemical pharmacology

authors： Chandoga J,Rojeková I,Hampl L,Hocman G

更新日期：1994-02-09 00:00:00

abstract：:Activation of protein kinase C (PKC) has been suggested to play a role in bone resorption. However, phorbol esters, which activate PKC, have been reported to have both stimulatory and inhibitory effects on bone resorption. To study the role of PKC in bone resorption further, we have measured calcium release elicited b...

journal_title：Biochemical pharmacology

authors： Lee SK,Stern PH

更新日期：2000-10-01 00:00:00

abstract：:2-Chloroacetaldehyde (CAA)-induced cytotoxicity in isolated hepatocytes was enhanced markedly if hepatocyte alcohol or aldehyde dehydrogenase was inhibited prior to CAA addition. Hepatocyte GSH depletion, ATP depletion and lipid peroxidation by CAA were also enhanced markedly. Furthermore, CAA was about 10- and 70-fol...

journal_title：Biochemical pharmacology

authors： Sood C,O'Brien PJ

更新日期：1994-08-30 00:00:00

abstract：:The present study was designed to evaluate the radioprotective effect of N- acetylcysteine (NAC) on gamma-radiation induced toxicity in hepatic tissue in rat. The cellular changes were estimated using malondialdehyde (MDA, an index of lipid peroxidation), superoxide dismutase (SOD), glutathione peroxidase (GSHPx), red...

journal_title：Biochemical pharmacology

authors： Mansour HH,Hafez HF,Fahmy NM,Hanafi N

更新日期：2008-02-01 00:00:00

abstract：:In the combat against inflammation, glucocorticoids (GCs) are a widespread therapeutic. These ligands of the glucocorticoid receptor (GR) inhibit the transactivation of various transcription factors, including nuclear factor-kappaB (NF-kappaB), and alter the composition of the pro-inflammatory enhanceosome, culminatin...

journal_title：Biochemical pharmacology

authors： Beck IM,Vanden Berghe W,Gerlo S,Bougarne N,Vermeulen L,De Bosscher K,Haegeman G

更新日期：2009-04-01 00:00:00

abstract：BACKGROUND AND PURPOSE:Chronic nicotine exposure upregulates α4β2* nicotinic acetylcholine receptors (nAChRs) in the brain. The goal of this study was to examine the role of three serine residues in the large cytoplasmic loop of the α4 subunit on α4β2* upregulation in neurons. EXPERIMENTAL APPROACH:Serine residues S33...

journal_title：Biochemical pharmacology

authors： Zambrano CA,Escobar D,Ramos-Santiago T,Bollinger I,Stitzel J

更新日期：2019-01-01 00:00:00

abstract：:Multivalent dendrimeric conjugates of GPCR ligands may have increased potency or selectivity in comparison to monomeric ligands, a phenomenon that was tested in a model of cytoprotection in mouse HL-1 cardiomyocytes. Quantitative RT-PCR indicated high expression levels of endogenous A(1) and A(2A) adenosine receptors ...

journal_title：Biochemical pharmacology

authors： Keene AM,Balasubramanian R,Lloyd J,Shainberg A,Jacobson KA

更新日期：2010-07-15 00:00:00

abstract：:During a cellular screening of thiocolchicine analogs, thiocolchicine dimers resulted particularly active in cisplatin-resistant A2780-CIS cells. In order to discover by which mechanism(s) thiocolchicine dimers overcame cisplatin resistance, p53, p21waf1 and MLH1 were assessed by Western blot. Results pointed out that...

journal_title：Biochemical pharmacology

authors： Raspaglio G,Ferlini C,Mozzetti S,Prislei S,Gallo D,Das N,Scambia G

更新日期：2005-01-01 00:00:00

abstract：:Intracellular GSH has some effects on protecting cells against cadmium and is involved in the development of resistance to cisplatin (CDDP). To determine the effects of intracellular GSH on expression of the heat shock genes (hsp) induced by cadmium in CDDP-resistant cancer cells, we used two human ovarian cancer cell...

journal_title：Biochemical pharmacology

authors： Abe T,Gotoh S,Higashi K

更新日期：1999-07-01 00:00:00

abstract：:Dimercaptosuccinic acid (DMSA) was shown to lower blood pressure in rat models of arterial hypertension. Thus, there is evidence that-besides its chelating properties-DMSA has a direct vascular effect, e.g. through scavenging of reactive oxygen species (ROS). We speculated that, in addition, intracellular calcium mobi...

journal_title：Biochemical pharmacology

authors： Kramer HJ,Mensikova V,Bäcker A,Meyer-Lehnert H,Gonick HC

更新日期：2003-05-15 00:00:00

abstract：:To investigate the potential interactions between the angiotensin II (Ang II) and insulin signaling systems, regulation of IRS-1 phosphorylation and insulin-induced Akt activation by Ang II were examined in clone 9 (C9) hepatocytes. In these cells, Ang II specifically inhibited activation of insulin-induced Akt Thr(30...

journal_title：Biochemical pharmacology

authors： Arellano-Plancarte A,Hernandez-Aranda J,Catt KJ,Olivares-Reyes JA

更新日期：2010-03-01 00:00:00

abstract：:Adherence of Plasmodium falciparum-infected erythrocytes (IE) to the venular endothelium in brain and other organs is characteristic of cerebral malaria, an often fatal complication in infected individuals. It has been shown that cytoadherence may be mediated through interaction of IE with glycoproteins on host target...

journal_title：Biochemical pharmacology

authors： Wright PS,Cross-Doersen DE,Schroeder KK,Bowlin TL,McCann PP,Bitonti AJ

更新日期：1991-06-15 00:00:00

abstract：:Two methods avoiding the widespread technique of collagenase perfusion have been employed to study the regulation of total cytochrome P450 content in rat hepatocyte culture. One technique required the perfusion of the liver with the chelating agent EDTA to dissociate the parenchymal cells prior to culture. Over a peri...

journal_title：Biochemical pharmacology

authors： Wright MC,Paine AJ

更新日期：1992-01-22 00:00:00

abstract：UNLABELLED:ZD1839 ("Iressa"), a selective epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), is currently undergoing preclinical and clinical evaluation in several solid tumors. The present study aimed to assess the effect of ZD1839 in combination with oxaliplatin in the colon cancer cell lines HT-2...

journal_title：Biochemical pharmacology

authors： Xu JM,Azzariti A,Severino M,Lu B,Colucci G,Paradiso A

更新日期：2003-08-15 00:00:00

abstract：:Bernserazide (D,L-serine 2-[2,3,4-trihydroxybenzyl]-hydrazide) as been shown to inhibit the clorgyline-resistant amine oxidase (CRAO) activities which metabolize benzylamine in homogenates of rat aorta, heart and brown adipose tissue. In vitro studies showed a concentration- and time-dependent inhibition of CRAO in he...

journal_title：Biochemical pharmacology

authors： Lyles GA,Callingham BA

更新日期：1982-04-01 00:00:00

abstract：:The side effects and low bioavailability of paclitaxel (PTX) limit its clinical application. The formation of self-assembled nanomedicines without structural modification is attractive for biomedical applications. Here, we constructed a supramolecular co-assembled nanoparticles (NPs), which is formed by betulonic acid...

journal_title：Biochemical pharmacology

authors： Wang J,Qiao W,Zhao H,Yang X

更新日期：2020-12-01 00:00:00

abstract：:A number of synthetic manganese complexes exhibit both in vitro and in vivo catalytic antioxidant activities. This study reports that the antioxidant potencies of a new series of meso-N,N'-dialkyl-imidazolium substituted manganese(III) porphyrins are dependent, in part, on their ability to redox cycle with endogenous ...

journal_title：Biochemical pharmacology

authors： Kachadourian R,Johnson CA,Min E,Spasojevic I,Day BJ

更新日期：2004-01-01 00:00:00

abstract：:It is increasingly clear that some of the effects of both free and derivatised long chain fatty acids in pancreatic beta-cells are mediated by a group of G-protein coupled receptors. Some of these display close structural homology while others are more divergent. This Commentary reviews the expression and functional r...

journal_title：Biochemical pharmacology

authors： Morgan NG,Dhayal S

更新日期：2009-12-15 00:00:00