Abstract:
:Here we have investigated the function of TcRho1, a Rho family orthologue from the parasite Trypanosoma cruzi. We have selected parasites overexpressing wild-type TcRho1 and a truncated form of TcRho1 (TcRho1-DeltaCaaX) which is unable to undergo farnesylation and supposed to interfere with recruitment of Rho effectors to membranes. TcRho1 protein was localized at the anterior region of wild-type and TcRho1 overexpressing epimastigotes, suggesting association with the Golgi apparatus. Accordingly, parasites overexpressing TcRho1-DeltaCaaX presented cytoplasmic fluorescence. To address the function of TcRho1 during differentiation, from epimastigotes to trypomastigotes, we submitted parasites overexpressing the above-cited lineages to metacyclogenesis assays. Parasites overexpressing TcRho1-DeltaCaaX generated a discrete number of metacyclic trypomastigotes when compared with other lineages. Strikingly, TcRho1-DeltaCaaX cells died synchronously during the process of metacyclogenesis.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
de Melo LD,Nepomuceno-Silva JL,Sant'Anna C,Eisele N,Ferraro RB,Meyer-Fernandes JR,de Souza W,Cunha-e-Silva NL,Lopes UGdoi
10.1016/j.bbrc.2004.08.197subject
Has Abstractpub_date
2004-10-22 00:00:00pages
1009-16issue
3eissn
0006-291Xissn
1090-2104pii
S0006-291X(04)01989-8journal_volume
323pub_type
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