Abstract：
:Using the fluorescent indicators 2',7'-bis(2-carboxyethyl)-5'-(6')-carboxyfluorescein and Oxonol V to monitor intracellular pH (pHi) and cell membrane potential respectively, we have investigated the involvement of H(+)-dependent ATPase and H(+)-dependent K+ channels in the recovery of the rat thyroid cell strain FRTL-5 from experimentally induced cytosolic acidification and membrane hyperpolarization events. Following exposure of cells to the weak acid sodium butyrate (24 mmol/l) under bicarbonate-free incubation conditions, cytoplasmic acidification was maximal after 3 min, attaining a pHi of 6.42. The subsequent recovery of pHi was unimpaired by the absence of extracellular K+, but was reduced in the presence of the Na+ antagonist amiloride (1 mmol/l), recovering by 0.11 +/- 0.003 units, compared with 0.27 +/- 0.02 units under amiloride-free conditions. In the presence of the H(+)-dependent ATPase antagonist N,N'-dicyclohexylcarbodiimide (DCC), the pHi recovery observed in amiloride-containing, K(+)-free buffer was abolished. The recovery of pHi in Na(+)- and K(+)-containing buffer was accompanied by hyperpolarization of the cell membrane, the later stage of which was reduced after blockade of K+ channels with BaCl2, implying a major contribution of transmembrane K+ movement to such events. In contrast to its attenuating effect on pHi recovery, DCC was ineffective in reducing butyrate-dependent membrane hyperpolarization, suggesting that H(+)-dependent ATPase may not be a major contributory factor to this event. However, when K+ channels were blocked by addition of BaCl2, addition of DCC abolished the butyrate-induced membrane depolarization.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
J Mol Endocrinoljournal_title
Journal of molecular endocrinologyauthors
Woods DJ,Soden J,Bidey SPdoi
10.1677/jme.0.0080079subject
Has Abstractpub_date
1992-02-01 00:00:00pages
79-86issue
1eissn
0952-5041issn
1479-6813journal_volume
8pub_type
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journal_title：Journal of molecular endocrinology
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更新日期：2009-03-01 00:00:00
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journal_title：Journal of molecular endocrinology
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更新日期：1992-04-01 00:00:00
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journal_title：Journal of molecular endocrinology
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doi：10.1677/jme.0.0320703
更新日期：2004-06-01 00:00:00
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journal_title：Journal of molecular endocrinology
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journal_title：Journal of molecular endocrinology
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