Homologous recombination in Candida albicans: role of CaRad52p in DNA repair, integration of linear DNA fragments and telomere length.

Abstract:

:Chromosomal rearrangements are common in both clinical isolates and spontaneous mutants of Candida albicans. It appears that many of these rearrangements are caused by translocations around the major sequence repeat (MSR) that is present in all chromosomes except chromosome 3, suggesting that homologous recombination (HR) may play an important role in the survival of this organism. In order to gain information on these processes, we have cloned the homologue of RAD52, which in Saccharomyces cerevisiae is the only gene required for all HR events. CaRAD52 complemented poorly a rad52 mutant of S. cerevisiae. Two null Carad52Delta/Carad52Delta mutants were constructed by sequential deletion of both alleles and two reconstituted strains were obtained by reintegration of the gene. Characterization of these mutants indicated that HR plays an essential role in the repair of DNA lesions caused by both UV light and the radiomimetic compound methyl-methane-sulphonate (MMS), whereas the non-homologous end-joining pathway (NHEJ) is used only in the absence of Rad52p or after extensive DNA damage. Repair by HR is more efficient in exponentially growing than in stationary cells, probably because a larger number of cells are in late S or G2 phases of the cell cycle (and therefore, can use a sister chromatid as a substrate for recombinational repair), whereas stationary phase cells are mainly in G0 or G1, and only can be repaired using the chromosomal homologue. In addition, CaRad52p is absolutely required for the integration of linear DNA with long flanking homologous sequences. Finally, the absence of CaRad52p results in the lengthening of telomeres, even in the presence of an active telomerase, an observation not described in any other organism. This raises the possibility that both telomerase and homologous recombination may function simultaneously at C. albicans telomeres.

journal_name

Mol Microbiol

journal_title

Molecular microbiology

authors

Ciudad T,Andaluz E,Steinberg-Neifach O,Lue NF,Gow NA,Calderone RA,Larriba G

doi

10.1111/j.1365-2958.2004.04197.x

subject

Has Abstract

pub_date

2004-08-01 00:00:00

pages

1177-94

issue

4

eissn

0950-382X

issn

1365-2958

pii

MMI4197

journal_volume

53

pub_type

杂志文章
  • Independent acquisition and insertion into different chromosomal locations of the same pathogenicity island in Yersinia pestis and Yersinia pseudotuberculosis.

    abstract::We show that Yersinia pestis and pesticin-sensitive isolates of Y. pseudotuberculosis possess a common 34 kbp DNA region that has all the hallmarks of a pathogenicity island and is inserted into different asparaginyl tRNA genes at different chromosomal locations in each species. This pathogenicity island (YP-HPI) is m...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1999.01172.x

    authors: Hare JM,Wagner AK,McDonough KA

    更新日期:1999-01-01 00:00:00

  • Phosphorylation-mediated regulation of heat shock response in Escherichia coli.

    abstract::Escherichia coli has two heat shock regulons under the transcriptional control of Esigma32 and EsigmaE RNA polymerases. These polymerases control the expression of genes, the products of which are needed for correct folding of proteins in the cytoplasm and the extracytoplasm respectively. In this study, we report that...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2003.03449.x

    authors: Klein G,Dartigalongue C,Raina S

    更新日期:2003-04-01 00:00:00

  • Strategies in pathogenesis: mechanistic specificity in the detection of generic signals.

    abstract::The virulence genes of the plant pathogen Agrobacterium tumefaciens are induced by more than 40 low-molecular-weight phenolic compounds. The prevailing opinion is that (i) wound-derived phenols produced on breach of the integrity of the cell wall act as the initiating signal in a series of events which results in host...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2958.1993.tb01155.x

    authors: Duban ME,Lee K,Lynn DG

    更新日期:1993-03-01 00:00:00

  • An essential role for phoP in Mycobacterium tuberculosis virulence.

    abstract::Two-component regulatory proteins function in bacteria as sensory and adaptive factors in response to a wide range of environmental stimuli. Some two-component systems, such as PhoP/PhoQ, control transcription of key virulence genes essential for survival in host cells in diverse intracellular bacterial pathogens, inc...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2001.02500.x

    authors: Pérez E,Samper S,Bordas Y,Guilhot C,Gicquel B,Martín C

    更新日期:2001-07-01 00:00:00

  • Polynucleotide phosphorylase is required for the rapid degradation of the RNase E-processed rpsO mRNA of Escherichia coli devoid of its 3' hairpin.

    abstract::The monocistronic transcript of rpsO undergoes an endonucleolytic cleavage downstream of the coding sequence, which removes the hairpin of the transcription terminator and initiates the rapid degradation of the message. We demonstrate here that the two rne-dependent cleavages, on both sides of the transcription termin...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1996.440971.x

    authors: Braun F,Hajnsdorf E,Régnier P

    更新日期:1996-03-01 00:00:00

  • Small mobile sequences in bacteria display diverse structure/function motifs.

    abstract::Small repeat sequences in bacterial genomes, which represent non-autonomous mobile elements, have close similarities to archaeon and eukaryotic miniature inverted repeat transposable elements. These repeat elements are found in both intergenic and intragenic chromosomal regions, and contain an array of diverse motifs....

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2958.2007.06068.x

    authors: Delihas N

    更新日期:2008-02-01 00:00:00

  • Identification and characterization of the pupB gene encoding an inducible ferric-pseudobactin receptor of Pseudomonas putida WCS358.

    abstract::Pseudomonas putida WCS358 can transport iron complexed to a wide variety of pseudobactins produced by other Pseudomonas strains. The pupB gene encoding an outer membrane ferric-pseudobactin receptor was isolated from a genomic library of P. putida WCS358. The PupB receptor facilitated iron transport via two distinct h...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1993.tb01603.x

    authors: Koster M,van de Vossenberg J,Leong J,Weisbeek PJ

    更新日期:1993-05-01 00:00:00

  • Non-canonical roles of tRNAs and tRNA mimics in bacterial cell biology.

    abstract::Transfer RNAs (tRNAs) are the macromolecules that transfer activated amino acids from aminoacyl-tRNA synthetases to the ribosome, where they are used for the mRNA guided synthesis of proteins. Transfer RNAs are ancient molecules, perhaps even predating the existence of the translation machinery. Albeit old, these mole...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/mmi.13419

    authors: Katz A,Elgamal S,Rajkovic A,Ibba M

    更新日期:2016-08-01 00:00:00

  • Activation of biosynthetic gene clusters by the global transcriptional regulator TRI6 in Fusarium graminearum.

    abstract::In F. graminearum, the transcription factor TRI6 positively regulates the trichothecene biosynthetic gene cluster (BGC) leading to the production of the secondary metabolite 15-acetyl deoxynivalenol. Secondary metabolites are not essential for survival, instead, they enable the pathogen to successfully infect its host...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.14575

    authors: Shostak K,Bonner C,Sproule A,Thapa I,Shields SWJ,Blackwell B,Vierula J,Overy D,Subramaniam R

    更新日期:2020-10-01 00:00:00

  • Identification of structural and molecular determinants of the tyrosine-kinase Wzc and implications in capsular polysaccharide export.

    abstract::Capsular polysaccharides are well-established virulence factors of pathogenic bacteria. Their biosynthesis and export are regulated within the transmembrane polysaccharide assembly machinery by the autophosphorylation of atypical tyrosine-kinases, named BY-kinases. However, the accurate functioning of these tyrosine-k...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2010.07291.x

    authors: Bechet E,Gruszczyk J,Terreux R,Gueguen-Chaignon V,Vigouroux A,Obadia B,Cozzone AJ,Nessler S,Grangeasse C

    更新日期:2010-09-01 00:00:00

  • Molecular analysis of the psa permease complex of Streptococcus pneumoniae.

    abstract::The psaBCA locus of Streptococcus pneumoniae encodes a putative ABC Mn2+-permease complex. Downstream of the operon is psaD, which may be co-transcribed and encodes a thiol peroxidase. Previously, there has been discordance concerning the phenotypic impact of mutations in the psa locus, resolution of which has been co...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2004.04164.x

    authors: McAllister LJ,Tseng HJ,Ogunniyi AD,Jennings MP,McEwan AG,Paton JC

    更新日期:2004-08-01 00:00:00

  • The transcription of bradyzoite genes in Toxoplasma gondii is controlled by autonomous promoter elements.

    abstract::Experimental evidence suggests that apicomplexan parasites possess bipartite promoters with basal and regulated cis-elements similar to other eukaryotes. Using a dual luciferase model adapted for recombinational cloning and use in Toxoplasma gondii, we show that genomic regions flanking 16 parasite genes, which encomp...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2008.06249.x

    authors: Behnke MS,Radke JB,Smith AT,Sullivan WJ Jr,White MW

    更新日期:2008-06-01 00:00:00

  • The bacterial condensin/cohesin-like protein complex acts in DNA repair and regulation of gene expression.

    abstract::Structural maintenance of chromosome (SMC) and the SMC-interacting kleisin protein families have key functions in the chromosome organization of most organisms. Here, we report that the Bacillus subtilis kleisin, ScpA, can form a ternary complex with the SMC and ScpB proteins in a yeast tri-hybrid assay, supporting th...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2003.03951.x

    authors: Dervyn E,Noirot-Gros MF,Mervelet P,McGovern S,Ehrlich SD,Polard P,Noirot P

    更新日期:2004-03-01 00:00:00

  • The free and bound forms of Lpp occupy distinct subcellular locations in Escherichia coli.

    abstract::The lipoprotein Lpp is the most numerically abundant protein in Escherichia coli, has been investigated for over 40 years, and has served as the paradigmatic bacterial lipoprotein since its initial discovery. It exists in two distinct forms: a 'bound-form', which is covalently bound to the cell's peptidoglycan layer, ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2011.07539.x

    authors: Cowles CE,Li Y,Semmelhack MF,Cristea IM,Silhavy TJ

    更新日期:2011-03-01 00:00:00

  • In vitro interaction between components of the inner membrane complex of the maltose ABC transporter of Escherichia coli: modulation by ATP.

    abstract::Interactions between domains of ATP-binding cassette (ABC) transporters are of great functional importance and yet are poorly understood. To gain further knowledge of these protein-protein interactions, we studied the inner membrane complex of the maltose transporter of Escherichia coli. We focused on interactions bet...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1998.01070.x

    authors: Mourez M,Jéhanno M,Schneider E,Dassa E

    更新日期:1998-10-01 00:00:00

  • Temperature sensing in bacterial gene regulation--what it all boils down to.

    abstract::Many bacterial gene regulatory circuits are controlled by temperature. Temperature-mediated regulation occurs at the level of transcription and translation. Supercoiling, changes in mRNA conformation and protein conformation are all implicated in thermosensing. Bacterial virulence functions are often temperature regul...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审

    doi:10.1046/j.1365-2958.1998.01049.x

    authors: Hurme R,Rhen M

    更新日期:1998-10-01 00:00:00

  • What are archaebacteria: life's third domain or monoderm prokaryotes related to gram-positive bacteria? A new proposal for the classification of prokaryotic organisms.

    abstract::The evolutionary relationship within prokaryotes is examined based on signature sequences (defined as conserved inserts or deletions shared by specific taxa) and phylogenies derived from different proteins. Archaebacteria are indicated as being monophyletic by a number of proteins related to the information transfer p...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审

    doi:10.1046/j.1365-2958.1998.00978.x

    authors: Gupta RS

    更新日期:1998-08-01 00:00:00

  • A novel RNA polymerase-binding protein controlling genes involved in spore germination in Bacillus subtilis.

    abstract::A growing class of proteins regulates transcription through interaction with DNA-dependent RNA polymerase. Here we report that a recently identified, highly conserved sporulation gene ylyA encodes a novel RNA polymerase-binding protein that influences the expression of genes under the control of the late-acting, sporu...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.12262

    authors: Traag BA,Ramirez-Peralta A,Wang Erickson AF,Setlow P,Losick R

    更新日期:2013-07-01 00:00:00

  • Reversible non-genetic phenotypic heterogeneity in bacterial quorum sensing.

    abstract::Bacteria co-ordinate their social behaviour in a density-dependent manner by production of diffusible signal molecules by a process known as quorum sensing (QS). It is generally assumed that in homogenous environments and at high cell density, QS synchronizes cells in the population to perform collective social tasks ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.12575

    authors: Pradhan BB,Chatterjee S

    更新日期:2014-05-01 00:00:00

  • Analysis of a cloned sequence of Legionella pneumophila encoding a 38 kD metalloprotease possessing haemolytic and cytotoxic activities.

    abstract::The DNA encoding the zinc metalloprotease of Legionella pneumophila Philadelphia 1 has been isolated and expressed in Escherichia coli. This protein, which is 38,000 Daltons in size, possesses immunological and biochemical properties identical to those previously described for the purified L. pneumophila protease. Per...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1989.tb00228.x

    authors: Quinn FD,Tompkins LS

    更新日期:1989-06-01 00:00:00

  • An antisense RNA controls synthesis of an SOS-induced toxin evolved from an antitoxin.

    abstract::Only few small, regulatory RNAs encoded opposite another gene have been identified in bacteria. Here, we report the characterization of a locus where a small RNA (SymR) is encoded in cis to an SOS-induced gene whose product shows homology to the antitoxin MazE (SymE). Synthesis of the SymE protein is tightly repressed...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2007.05688.x

    authors: Kawano M,Aravind L,Storz G

    更新日期:2007-05-01 00:00:00

  • The Rhodobacter capsulatus hupSLC promoter: identification of cis-regulatory elements and of trans-activating factors involved in H2 activation of hupSLC transcription.

    abstract::The [NiFe]hydrogenase of the photosynthetic bacterium Rhodobacter capsulatus is encoded by the structural hupSLC operon, the expression of which is induced by H2. H2 activation was no longer observable in chromosomal hupR mutants, an indication that HupR is implicated directly in the activation by H2 of hupS gene expr...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1997.6291996.x

    authors: Toussaint B,de Sury d'Aspremont R,Delic-Attree I,Berchet V,Elsen S,Colbeau A,Dischert W,Lazzaroni Y,Vignais PM

    更新日期:1997-12-01 00:00:00

  • The inflammation-associated Salmonella SopA is a HECT-like E3 ubiquitin ligase.

    abstract::Salmonella translocate a group of type III effectors into the host cells to induce entry, promote survival and cause intestinal inflammation. Although the biochemical and cellular mechanisms of how bacterial effectors function inside host cells remain largely unknown, studies have indicated that a likely strategy is t...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2006.05407.x

    authors: Zhang Y,Higashide WM,McCormick BA,Chen J,Zhou D

    更新日期:2006-11-01 00:00:00

  • Pseudomonas aeruginosa defense systems against microbicidal oxidants.

    abstract::The most abundant oxidants controlling bacterial colonization on mucosal barrier epithelia are hypochlorous acid (HOCl), hypobromous acid (HOBr) and hypothiocyanous acid (HOSCN). All three oxidants are highly antimicrobial but little is known about their relative efficacies, their respective cellular targets, or what ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.13768

    authors: Groitl B,Dahl JU,Schroeder JW,Jakob U

    更新日期:2017-11-01 00:00:00

  • Three new RelE-homologous mRNA interferases of Escherichia coli differentially induced by environmental stresses.

    abstract::Prokaryotic toxin - antitoxin (TA) loci encode mRNA interferases that inhibit translation, either by cleaving mRNA codons at the ribosomal A site or by cleaving any RNA site-specifically. So far, seven mRNA interferases of Escherichia coli have been identified, four of which cleave mRNA by a translation-dependent mech...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2009.06969.x

    authors: Christensen-Dalsgaard M,Jørgensen MG,Gerdes K

    更新日期:2010-01-01 00:00:00

  • Integration host factor affects expression of two genes at the conjugal transfer origin of plasmid R100.

    abstract::Integration host factor (IHF) binds to two sites near the origin of transfer of the conjugative antibiotic resistance plasmid, R100. DNase I footprinting shows that one site is immediately adjacent to oriT and the gene X promoter, and another is adjacent to the traM promoter. A third site, known only from retardation ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1990.tb00674.x

    authors: Dempsey WB,Fee BE

    更新日期:1990-06-01 00:00:00

  • The complete nucleotide sequence of the Pasteurella multocida toxin gene and evidence for a transcriptional repressor, TxaR.

    abstract::The osteolytic toxin of Pasteurella multocida induces bone resorption in vivo and in vitro (Foged et al., 1988; Kimman et al., 1987). In this report the toxin-encoding toxA gene is sequenced, and the deduced primary structure of the toxin shows a protein of 1285 amino acids, containing a striking homology to a metal-b...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1990.tb00652.x

    authors: Petersen SK

    更新日期:1990-05-01 00:00:00

  • Open complex formation by Escherichia coli RNA polymerase: the mechanism of polymerase-induced strand separation of double helical DNA.

    abstract::Escherichia coli RNA polymerase is able to site-specifically melt 12 bp of promoter DNA at temperatures far below those normally associated with DNA melting. Here we consider several models to explain how RNA polymerase destabilizes duplex DNA. One popular model proposes that upon binding to the promoter, RNA polymera...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2958.1995.tb02309.x

    authors: deHaseth PL,Helmann JD

    更新日期:1995-06-01 00:00:00

  • Control of cell shape and elongation by the rodA gene in Bacillus subtilis.

    abstract::The Escherichia coli rodA and ftsW genes and the spoVE gene of Bacillus subtilis encode membrane proteins that control peptidoglycan synthesis during cellular elongation, division and sporulation respectively. While rodA and ftsW are essential genes in E. coli, the B. subtilis spoVE gene is dispensable for growth and ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1998.00766.x

    authors: Henriques AO,Glaser P,Piggot PJ,Moran CP Jr

    更新日期:1998-04-01 00:00:00

  • An isogenic haemolysin-deficient mutant of Haemophilus ducreyi lacks the ability to produce cytopathic effects on human foreskin fibroblasts.

    abstract::The haemolysin of Haemophilus ducreyi is the newest member of the Proteus/Serratia family of pore-forming toxins. In order to assess the role of the haemolysin in virulence, we constructed an isogenic haemolysin-deficient mutant of H. ducreyi strain 35000 This strain, designated 35000-3, lacks detectable haemolytic ac...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1996.00615.x

    authors: Palmer KL,Goldman WE,Munson RS Jr

    更新日期:1996-07-01 00:00:00