Endometrial lymphoid tissue in the timed endometrial biopsy: morphometric and immunohistochemical aspects.

Abstract:

OBJECTIVES:The purpose of this study was to provide a morphometric profile of endometrial granulated lymphocytes and to investigate qualitative and quantitative differences in leukocyte subsets in precisely timed luteal phase endometrial biopsies. STUDY DESIGN:Endometrial biopsies were obtained from 24 normal fertile women at 4, 7, 10, and 13 days after the luteinizing hormone surge. Endometrial granulated lymphocytes were assessed morphometrically in 2 microns resin sections. Eleven monoclonal antibodies were used to characterize leukocytes in frozen sections. Semiquantitation was performed with a Quantimet 970 image analyzer. Data were analyzed with one-way analysis of variance. RESULTS:CD8+ (T suppressor-cytotoxic) cells increased significantly from 4 to 7 days after the luteinizing hormone surge, whereas CD68+ macrophages increased from days 10 to 13. Lymphocytes with an unusual phenotype (CD56+, CD38+, CD2+) increased dramatically after 7 days. The volume fraction of endometrium occupied by the nuclei of endometrial granulated lymphocytes did not alter, but their mean nuclear diameter and axial ratio decreased from days 7 to 13. CONCLUSION:The morphometric findings indicate in situ proliferation of endometrial granulated lymphocytes rather than migration from the peripheral circulation. T lymphocytes, macrophages, and endometrial granulated lymphocytes increase significantly between certain stages of the luteal phase.

journal_name

Am J Obstet Gynecol

authors

Klentzeris LD,Bulmer JN,Warren A,Morrison L,Li TC,Cooke ID

doi

10.1016/s0002-9378(11)91568-3

subject

Has Abstract

pub_date

1992-09-01 00:00:00

pages

667-74

issue

3

eissn

0002-9378

issn

1097-6868

pii

S0002-9378(11)91568-3

journal_volume

167

pub_type

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