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Vanadate activates Rho A translocation in association with contracting effects in ileal longitudinal smooth muscle of guinea pig.

Abstract：

:We characterized the effects of vanadate, an inhibitor of tyrosine phosphatase, on the tension, the level of myosin light chain (MLC) phosphorylation, and Rho A activation in intact ileal longitudinal smooth muscle of the guinea pig to study the role of tyrosine phosphorylation in contraction signaling. Vanadate exerted a sustained contraction with a slow onset of tension development, in a concentration-dependent manner. The contractile effects of vanadate were accompanied by increases in the level of MLC phosphorylation. The tyrosine kinase inhibitor genistein; the MLC kinase inhibitor 1-(5-chloronaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine hydrochloride (ML-9); and the Rho kinase inhibitor (+)-(R)-trans-4-(1-aminoethyl)-N-(4-pyridyl) cyclohexanecarboxamide dihydrochloride, monohydrate (Y-27632) inhibited the vanadate-induced contraction and MLC phosphorylation. Vanadate caused Rho A translocation from the cytosol to the membrane fraction, which was inhibited by genistein, but not by ML-9 and Y-27632. These data indicate that vanadate induces Rho A activation probably via protein tyrosine phosphorylation and the subsequent contraction through increases in the level of MLC phosphorylation.

journal_name

J Pharmacol Sci

journal_title

Journal of pharmacological sciences

authors

Mori M,Tsushima H

doi

10.1254/jphs.fp0030576

subject

Has Abstract

pub_date

2004-08-01 00:00:00

pages

443-51

issue

4

eissn

1347-8613

issn

1347-8648

pii

JST.JSTAGE/jphs/FP0030576

journal_volume

95

pub_type

杂志文章

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