Application of a molecular beacon-real-time PCR technology to detect Salmonella species contaminating fruits and vegetables.

Abstract:

:An oligonucleotide probe that becomes fluorescent upon hybridization to the target DNA (molecular beacon; MB) was evaluated in a real-time polymerase chain reaction (PCR) assay to detect the presence of Salmonella species. As few as 1-4 colony-forming units (CFU) per PCR reaction could be detected. The capability of the assay to detect Salmonella species from artificially inoculated fresh-cut produce such as cantaloupe, mixed-salad, cilantro, and alfalfa sprouts was demonstrated. A comparison of two commercially available kits utilizing MB-PCR (iQ-Check, Bio-Rad Laboratories) and conventional Association of Official Analytical Chemists (AOAC)-approved PCR (BAX, Dupont Qualicon) was performed on artificially inoculated produce. As few as 4 CFU/25 g of produce were detected after 16 h of enrichment in buffered peptone broth. These assays could be carried out entirely in sealed PCR tubes, enabling a rapid and high-throughput detection of Salmonella species in a large number of food and environmental samples. This is the first report of the application of MB probe being used for real-time detection of Salmonella species in whole and fresh-cut fruits and vegetables.

journal_name

Int J Food Microbiol

authors

Liming SH,Bhagwat AA

doi

10.1016/j.ijfoodmicro.2004.02.013

subject

Has Abstract

pub_date

2004-09-01 00:00:00

pages

177-87

issue

2

eissn

0168-1605

issn

1879-3460

pii

S0168160504001175

journal_volume

95

pub_type

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