Effect of short-term training on GLUT-4 mRNA and protein expression in human skeletal muscle.

Abstract:

:Six untrained, male subjects (23 +/- 1 years old, 84 +/- 5 kg, (O(2)peak)= 3.7 +/- 0.8 l min(-1)) exercised for 60 min at 75 +/- 1%(O(2)peak) on 7 consecutive days. Muscle samples were obtained before the start of cycle exercise training and 24 h after the first and seventh exercise sessions and analysed for citrate synthase activity, glycogen and glucose transporter 4 (GLUT-4) mRNA and protein expression. Exercise training increased (P < 0.05) citrate synthase by approximately 20% and muscle glycogen concentration by approximately 40%. GLUT-4 mRNA levels 24 h after the first and seventh exercise sessions were similar to those measured before the start of exercise training. In contrast, GLUT-4 protein expression was increased after 7 days of exercise training (12.4 +/- 1.5 versus 3.4 +/- 1.0 arbitray units (a.u.), P < 0.05) and although it tended to be higher 24 h after the first exercise session (6.0 +/- 3.0 versus 3.4 +/- 1.0 a.u.), this was not significantly different (P= 0.09). These results support the suggestion that the adaptive increase in skeletal muscle GLUT-4 protein expression with short-term exercise training arises from the repeated, transient increases in GLUT-gene transcription following each exercise bout leading to a gradual accumulation of GLUT-4 protein, despite GLUT-4 mRNA returning to basal levels between exercise stimuli.

journal_name

Exp Physiol

journal_title

Experimental physiology

authors

Kraniou GN,Cameron-Smith D,Hargreaves M

doi

10.1113/expphysiol.2004.027409

subject

Has Abstract

pub_date

2004-09-01 00:00:00

pages

559-63

issue

5

eissn

0958-0670

issn

1469-445X

pii

expphysiol.2004.027409

journal_volume

89

pub_type

杂志文章
  • Eccentric cardiac hypertrophy was induced by long-term intermittent hypoxia in rats.

    abstract::It is unclear whether cardiac hypertrophy and hypertrophy-related pathways will be induced by long-term intermittent hypoxia. Thirty-six Sprague-Dawley rats were randomly assigned into three groups: normoxia, and long-term intermittent hypoxia (12% O(2), 8 h per day) for 4 weeks (4WLTIH) or for 8 weeks (8WLTIH). Myoca...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.2006.036590

    authors: Chen LM,Kuo WW,Yang JJ,Wang SG,Yeh YL,Tsai FJ,Ho YJ,Chang MH,Huang CY,Lee SD

    更新日期:2007-03-01 00:00:00

  • Intervisceral artery origins in patients with abdominal aortic aneurysmal disease; evidence for systemic vascular remodelling.

    abstract:NEW FINDINGS:What is the central question of this study? To what extent focal abdominal aortic aneurysmal (AAA) disease is associated with systemic remodelling of the vascular tree remains unknown. The present study examined whether anatomical differences exist between distances of the intervisceral artery origins and ...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/EP085804

    authors: Bailey DM,Evans TG,Thomas KG,White RD,Twine CP,Lewis MH,Williams IM

    更新日期:2016-08-01 00:00:00

  • The role of exercise on long-term effects of alloxan administered in neonatal rats.

    abstract::The present study was designed to analyse the effects of aerobic exercise on the metabolic effects of alloxan. Male Wistar newborn rats (2 days old) received alloxan (200 mg (kg body weight)(-1)) intraperitoneally (A rats). Vehicle-injected rats were used as controls (C rats). At 28 days old, some of the A rats were s...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.2004.028241

    authors: de Oliveira CA,Luciano E,de Mello MA

    更新日期:2005-01-01 00:00:00

  • Endothelial hypoxic preconditioning in rat hypoxic isolated aortic segments.

    abstract::Our aim was to analyse endothelial hypoxic preconditioning after hypoxia-reperfusion (HR). Endothelial functionality was analysed through the vasorelaxation responses to acetylcholine (Ach) and the level of serine1177 phosphorylated endothelial nitric oxide synthase (eNOS) (ser1177-eNOS) measured by Western blot in in...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.2005.030163

    authors: Carrasco-Martín C,Alonso-Orgaz S,De la Pinta JC,Marques M,Macaya C,Barrientos A,González MM,García-Méndez A,Mateos-Cáceres PJ,Porres JC,Rico LA,López-Farré AJ

    更新日期:2005-07-01 00:00:00

  • Natural killer cell mobilization and egress following acute exercise in men with prostate cancer.

    abstract:NEW FINDINGS:What is the central question of this study? What are the characteristics of the NK cell response following acute moderate-intensity aerobic exercise in prostate cancer survivors and is there a relationship between stress hormones and NK cell mobilization? What is the main finding and its importance? NK cel...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/EP088627

    authors: Hanson ED,Sakkal S,Que S,Cho E,Spielmann G,Kadife E,Violet JA,Battaglini CL,Stoner L,Bartlett DB,McConell GK,Hayes A

    更新日期:2020-09-01 00:00:00

  • Adenosine and hypoxanthine transport in horse erythrocytes: evidence for a polymorphism in the transport of hypoxanthine via a sodium-dependent cotransporter.

    abstract::The inward transport of two purines, adenosine and hypoxanthine, at 37 degrees C by horse erythrocytes was compared. No mediated transport of adenosine was detected in horse erythrocytes, nor was saturable, high-affinity binding of the potent facilitated-diffusion inhibitor nitrobenzylthioinosine demonstrable in horse...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1998.sp004104

    authors: Jarvis SM,Harris RC

    更新日期:1998-03-01 00:00:00

  • Appearance of the capillary endothelial glycocalyx in chronically stimulated rat skeletal muscles in relation to angiogenesis.

    abstract::The endothelial glycocalyx layer of capillaries was stained with Ruthenium Red and examined by transmission electron microscopy in rat extensor hallucis proprius muscles after 2 or 4 days of indirect electrical stimulation via implanted electrodes. The layer appeared continuous on the luminal surface of 63% capillarie...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1996.sp003989

    authors: Brown MD,Egginton S,Hudlicka O,Zhou AL

    更新日期:1996-11-01 00:00:00

  • Long non-coding RNA activated by transforming growth factor-β promotes proliferation and invasion of cervical cancer cells by regulating the miR-144/ITGA6 axis.

    abstract:NEW FINDINGS:What is the central question of this study? This study was designed to investigate the molecular mechanism and biological roles of long non-coding RNA activated by transforming growth factor-β (lncRNA ATB) in the progression of cervical cancer. What is the main finding and its importance? Our study provide...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/EP087656

    authors: Zhu Y,Wu Y,Yang L,Dou X,Jiang J,Wang L

    更新日期:2019-06-01 00:00:00

  • Reduced hypoxic ventilatory response in newborn mice knocked-out for the progesterone receptor.

    abstract::Recent studies showed that progesterone stimulates the hypoxic ventilatory response and may reduce apnoea frequency in newborn rats, but so far we still do not know by what mechanisms and whether endogenous progesterone might contribute to respiratory control in neonates. We therefore determined the role of the nuclea...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.2014.080986

    authors: Potvin C,Rossignol O,Uppari N,Dallongeville A,Bairam A,Joseph V

    更新日期:2014-11-01 00:00:00

  • A monocarboxylate transporter MCT1 is located at the basolateral pole of rat jejunum.

    abstract::We have functionally expressed and identified a monocarboxylate transporter (MCT1) from rat jejunal enterocyte and we provide evidence for its basolateral localization. Poly(A)+ RNA isolated from rat jejunum was injected into Xenopus laevis oocytes and expression of a proton-lactate symporter was investigated by means...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:

    authors: Orsenigo MN,Tosco M,Bazzini C,Laforenza U,Faelli A

    更新日期:1999-11-01 00:00:00

  • Confocal analysis of fluorescent bead uptake by mouse Peyer's patch follicle-associated M cells.

    abstract::Latex beads coated with secretory immunoglobulin (IgA) instilled into mouse intestinal loops are taken up by M cells present in Peyer's patch follicle-associated epithelial tissue. Bead adsorption and uptake is greater at the edge compared with the apex of follicle domes. Coating beads with bovine serum albumin (BSA) ...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1992.sp003662

    authors: Porta C,James PS,Phillips AD,Savidge TC,Smith MW,Cremaschi D

    更新日期:1992-11-01 00:00:00

  • White blood cell concentrations during lower body negative pressure and blood loss in humans.

    abstract:NEW FINDINGS:What is the central question of this study? Is lower body negative pressure a useful surrogate to study white blood cell responses to haemorrhage in humans? What is the main finding and its importance? We found that lower body negative pressure appears to be a useful surrogate to study the early white bloo...

    journal_title:Experimental physiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1113/EP085952

    authors: van Helmond N,Johnson BD,Curry TB,Cap AP,Convertino VA,Joyner MJ

    更新日期:2016-10-01 00:00:00

  • Human hypertension, sympathetic activity and the selfish brain.

    abstract:NEW FINDINGS:What is the topic of this review? This review article revisits an historical hypothesis that cerebral hypoperfusion, caused by elevated cerebral vascular resistances, causes the onset of high sympathetic nerve activity and hypertension in humans. What advances does it highlight? The review article highligh...

    journal_title:Experimental physiology

    pub_type: 杂志文章,评审

    doi:10.1113/EP085775

    authors: Hart EC

    更新日期:2016-12-01 00:00:00

  • Homeobox A5 and C10 genes modulate adaptation of brown adipose tissue during exercise training in juvenile rats.

    abstract:NEW FINDINGS:What is the central question of this study? Exercise can stimulate brown adipose tissue (BAT) with subsequent increase in uncoupling protein 1 expression and mitochondrial biogenesis. In that case, do BAT-specific Hox genes modify BAT functioning and cause uncoupling protein expression changes due to exerc...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/EP089114

    authors: Kato H,Ario T,Kishida T,Tadano M,Osawa S,Maeda Y,Takakura H,Izawa T

    更新日期:2020-12-28 00:00:00

  • Mineralocorticoid receptors, inflammation and sympathetic drive in a rat model of systolic heart failure.

    abstract::Appreciation for the role of aldosterone and mineralocorticoid receptors in cardiovascular disease is accelerating rapidly. Recent experimental work has unveiled a strong relationship between brain mineralocorticoid receptors and sympathetic drive, an important determinant of outcome in heart failure and hypertension....

    journal_title:Experimental physiology

    pub_type: 杂志文章,评审

    doi:10.1113/expphysiol.2008.045948

    authors: Felder RB

    更新日期:2010-01-01 00:00:00

  • Glycopyrrolate abolishes the exercise-induced increase in cerebral perfusion in humans.

    abstract::Brain blood vessels contain muscarinic receptors that are important for cerebral blood flow (CBF) regulation, but whether a cholinergic receptor mechanism is involved in the exercise-induced increase in cerebral perfusion or affects cerebral metabolism remains unknown. We evaluated CBF and cerebral metabolism (from ar...

    journal_title:Experimental physiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1113/expphysiol.2010.054346

    authors: Seifert T,Fisher JP,Young CN,Hartwich D,Ogoh S,Raven PB,Fadel PJ,Secher NH

    更新日期:2010-10-01 00:00:00

  • Mapping the central neurocircuitry that integrates the cardiovascular response to exercise in humans.

    abstract::There are abundant animal data attempting to identify the neural circuitry involved in cardiovascular control. Translating this research into humans has been made possible using functional neurosurgery during which deep brain stimulating electrodes are implanted into various brain nuclei for the treatment of chronic p...

    journal_title:Experimental physiology

    pub_type: 杂志文章,评审

    doi:10.1113/expphysiol.2011.060848

    authors: Basnayake SD,Green AL,Paterson DJ

    更新日期:2012-01-01 00:00:00

  • Ghrelin ameliorates blood-brain barrier disruption during systemic hypoxia.

    abstract:NEW FINDINGS:What is the central question of this study? Is an anti-oedematous effect of ghrelin associated with increased expression of tight junction proteins in the hypoxic brain? What is the main finding and its importance? We showed that injection of ghrelin during acute and chronic systemic hypoxia is associated ...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/EP086068

    authors: Mohaddes G,Abdolalizadeh J,Babri S,Hossienzadeh F

    更新日期:2017-03-01 00:00:00

  • Human amniotic membrane mesenchymal stem cell-conditioned medium reduces inflammatory factors and fibrosis in ovalbumin-induced asthma in mice.

    abstract:NEW FINDINGS:What is the central question of this study? Is mesenchymal stem cell-conditioned medium capable of improving the pathological alterations of ovalbumin-induced asthma in mice? What is the main finding and its importance? Our study indicated that human amniotic membrane mesenchymal stem cell-conditioned medi...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/EP088911

    authors: Dalouchi F,Falak R,Bakhshesh M,Sharifiaghdam Z,Azizi Y,Aboutaleb N

    更新日期:2020-12-01 00:00:00

  • Endogenous peripheral hydrogen sulfide is propyretic: its permissive role in brown adipose tissue thermogenesis in rats.

    abstract:NEW FINDINGS:What is the central question of this study? In fever, the most striking response in the acute phase reaction of systemic inflammation, plasma H2 S concentration increases. However, the role of endogenous peripheral H2 S in fever is unknown. What is the main finding and its importance? Endogenous peripheral...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/EP086775

    authors: Soriano RN,Braga SP,Breder JSC,Batalhao ME,Oliveira-Pelegrin GR,Ferreira LFR,Rocha MJA,Carnio EC,Branco LGS

    更新日期:2018-03-01 00:00:00

  • Carotid body chemoreflex: a driver of autonomic abnormalities in sleep apnoea.

    abstract::What is the topic of this review? This article presents emerging evidence for heightened carotid body chemoreflex activity as a major driver of sympathetic activation and hypertension in sleep apnoea patients. What advances does it heighlight? This article discusses the recent advances on cellular, molecular and epige...

    journal_title:Experimental physiology

    pub_type: 杂志文章,评审

    doi:10.1113/EP085624

    authors: Prabhakar NR

    更新日期:2016-08-01 00:00:00

  • Cerebral haemodynamics and oxygenation during whole-body exercise over 5 days at high altitude.

    abstract:NEW FINDINGS:What is the central question of this study? Impairment and subsequent improvement in cerebral oxygenation during acute and prolonged exposure to high altitude affect exercise performance. This study innovates by investigating the effect of acute and prolonged high-altitude exposure on cerebral haemodynamic...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/EP088354

    authors: Marillier M,Rupp T,Bouzat P,Walther G,Baillieul S,Millet GY,Robach P,Verges S

    更新日期:2021-01-01 00:00:00

  • Neuronal circuits in the hypothalamus controlling gonadotrophin-releasing hormone release: the neuroanatomical projections of kisspeptin neurons.

    abstract::The hypothalamus regulates the key hormonal signalling events essential for reproduction and fertility in mammals. The gonadotrophin-releasing hormone (GnRH) neurons are the principal neurons of the complex neuronal network that co-ordinates multiple internal homeostatic and external factors necessary for fertility. K...

    journal_title:Experimental physiology

    pub_type: 杂志文章,评审

    doi:10.1113/expphysiol.2013.071944

    authors: Yeo SH

    更新日期:2013-11-01 00:00:00

  • Peroxidase and kallikrein in atropine-resistant secretion of submandibular saliva on parasympathetic nerve stimulation in anaesthetized rats.

    abstract::Flow of submandibular saliva and the constituents secreted during chorda-lingual nerve stimulation at 40 Hz (3 x 10 min) were studied after alpha- and beta-adrenergic blockade, and in the absence (control) or presence of cholinergic blockade in anaesthetized rats. Peroxidase and true tissue kallikrein (rK1) were asses...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1996.sp003940

    authors: Garrett JR,Anderson LC,Zhang XS,Proctor GB

    更新日期:1996-05-01 00:00:00

  • Inhibition of vasopressin and aldosterone release by atrial natriuretic peptide in conscious rabbits.

    abstract::To elucidate the regulatory role of atrial natriuretic factor (ANF) on vasopressin (AVP) and aldosterone release in conscious rabbits, ANF was administered systematically at a rate of 15 pmol min-1 (kg body wt)-1 for 15 min in two series of experimental animals in which AVP and/or aldosterone production was stimulated...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1992.sp003622

    authors: Gerstberger R,Schütz H,Luther-Dyroff D,Keil R,Simon E

    更新日期:1992-07-01 00:00:00

  • Current computational models do not reveal the importance of the nervous system in long-term control of arterial pressure.

    abstract::Arterial pressure is regulated over long periods of time by neural, hormonal and local control mechanisms, which ultimately determine the total blood volume and how it is distributed between the various vascular compartments of the circulation. A full understanding of the complex interplay of these mechanisms can be g...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.2008.043281

    authors: Osborn JW,Averina VA,Fink GD

    更新日期:2009-04-01 00:00:00

  • Influence of fitness and age on the endothelial response to acute inflammation.

    abstract:NEW FINDINGS:What is the central question of the study? What are the effects of age and fitness on the vascular response to acute inflammation in younger and older adults? What is the main finding and its importance? In older adults, cardiorespiratory fitness level has a differential impact on endothelial function afte...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/EP086922

    authors: Schroeder EC,Lane-Cordova AD,Ranadive SM,Baynard T,Fernhall B

    更新日期:2018-06-01 00:00:00

  • Heavy and moderate interval exercise training alters low-flow-mediated constriction but does not increase circulating progenitor cells in healthy humans.

    abstract::Moderate-intensity endurance exercise training improves vascular endothelial vasomotor function; however, the impact of high-intensity exercise training has been equivocal. Thus, the effect of the physiological stress of the exercise remains poorly understood. Furthermore, enhanced vascular repair mediated by circulat...

    journal_title:Experimental physiology

    pub_type: 临床试验,杂志文章

    doi:10.1113/expphysiol.2011.062836

    authors: Rakobowchuk M,Harris E,Taylor A,Baliga V,Cubbon RM,Rossiter HB,Birch KM

    更新日期:2012-03-01 00:00:00

  • Osteopetrosis in obese female rats is site-specifically inhibited by physical training.

    abstract:NEW FINDINGS:What is the central question of this study? Clinical studies suggest that obesity 'protects' against osteoporosis. However, these studies used only bone densitometry and assessed only one bone site, which is insufficient to enable conclusions to be drawn about the response of the whole skeleton. Furthermor...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.2014.082511

    authors: Lacerda DR,Serakides R,de Melo Ocarino N,Ferreira AV,Moraes MM,Boeloni JN,Silva JF,de Oliveira MC,de Barcellos LA,Rodrigues LO,Soares DD

    更新日期:2015-01-01 00:00:00

  • Extracellular calcium chelation and attenuation of calcium entry decrease in vivo cholinergic-induced eccrine sweating sensitivity in humans.

    abstract:NEW FINDINGS:What is the central question of this study? Calcium is an important second messenger in eccrine sweating; however, whether modulation of extracellular Ca(2+) and Ca(2+) entry has the capacity to modulate sweat rate in non-glabrous human skin has not been explored. What is the main finding and its importanc...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.2013.076547

    authors: Metzler-Wilson K,Sammons DL,Ossim MA,Metzger NR,Jurovcik AJ,Krause BA,Wilson TE

    更新日期:2014-02-01 00:00:00