Abstract:
:Platelet aggregation and microaggregate formation were measured in samples of stirred whole blood by flow cytometry. Blood samples were stirred in a multi-sample agitator with ADP, fixed and labelled with a platelet-specific CD42a-FITC fluorescent antibody. The blood was then diluted and applied directly to a flow cytometer. Platelets were identified using a gating procedure based on their expression of CD42a and then quantified. Aggregation was monitored as a fall in the number of single platelets. Both reversible and irreversible aggregation responses to ADP were determined and these were found to correlate directly with aggregation responses determined using a well-established single platelet counting technique using the Ultra-Flo 100 Whole Blood Platelet Counter. We found from flow cytometry that ADP-induced aggregation was coupled with a transient formation of platelet microaggregates over the initial 60 s following ADP addition. Assessment of single platelet loss by flow cytometry was found to be a reliable way of monitoring aggregation responses and provided new information on rapid microaggregate formation in ADP-stimulated blood.
journal_name
Plateletsjournal_title
Plateletsauthors
Fox SC,Sasae R,Janson S,May JA,Heptinstall Sdoi
10.1080/09537100310001645979subject
Has Abstractpub_date
2004-03-01 00:00:00pages
85-93issue
2eissn
0953-7104issn
1369-1635pii
UDHBF229B9AC53W6journal_volume
15pub_type
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