Mechanical stimulation promotes osteogenic differentiation of human bone marrow stromal cells on 3-D partially demineralized bone scaffolds in vitro.

Abstract:

:Bone is a dynamic tissue that is able to sense and adapt to mechanical stimuli by modulating its mass, geometry, and structure. Bone marrow stromal cells (BMSCs) are known to play an integral part in bone formation by providing an osteoprogenitor cell source capable of differentiating into mature osteoblasts in response to mechanical stresses. Characteristics of the in vivo bone environment including the three dimensional (3-D) lacunocanalicular structure and extracellular matrix composition have previously been shown to play major roles in influencing mechanotransduction processes within bone cells. To more accurately model this phenomenon in vitro, we cultured human BMSCs on 3-D, partially demineralized bone scaffolds in the presence of four-point bending loads within a novel bioreactor. The effect of mechanical loading and dexamethasone concentration on BMSC osteogenic differentiation and mineralized matrix production was studied for 8 and 16 days of culture. Mechanical stimulation after 16 days with 10 nM dexamethasone promoted osteogenic differentiation of BMSCs by significantly elevating alkaline phosphatase activity as well as alkaline phosphatase and osteopontin transcript levels over static controls. Mineralized matrix production also increased under these culture conditions. Dexamethasone concentration had a dramatic effect on the ability of mechanical stimulation to modulate these phenotypic and genotypic responses. These results provide increased insight into the role of mechanical stimulation on osteogenic differentiation of human BMSCs in vitro and may lead to improved strategies in bone tissue engineering.

journal_name

Calcif Tissue Int

authors

Mauney JR,Sjostorm S,Blumberg J,Horan R,O'Leary JP,Vunjak-Novakovic G,Volloch V,Kaplan DL

doi

10.1007/s00223-003-0104-7

subject

Has Abstract

pub_date

2004-05-01 00:00:00

pages

458-68

issue

5

eissn

0171-967X

issn

1432-0827

journal_volume

74

pub_type

杂志文章
  • Genetics of male osteoporosis.

    abstract::In the past years, several epidemiological and clinical observations have underlined the importance of genetics in the pathogenesis of both female and male osteoporosis. It has been estimated that from 50 to 80% of the inter-individual variability in bone mass is genetically determined. In rare instances, osteoporosis...

    journal_title:Calcified tissue international

    pub_type: 杂志文章,评审

    doi:10.1007/s00223-001-1049-3

    authors: Gennari L,Brandi ML

    更新日期:2001-10-01 00:00:00

  • A postmortem correlation of four techniques of assessment of osteoporosis with force of bone compression.

    abstract::Prosected vertebrae and phalanges from 32 cadavera were examined using noninvasive methods commonly used for assessment of osteoporosis. Bone measures were then compared with a strength variable obtained by mechanically crushing or breaking the bones. All of the phalangeal density assessment techniques were found to b...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02555150

    authors: Oyster N,Smith FW

    更新日期:1988-08-01 00:00:00

  • Cancellous bone of the spine is greater in black women.

    abstract::The bone mineral density (BMD) of the spine was measured in the posteroanterior (PA) and lateral projections as well as the total body BMD in 447 black and white women. The lateral projection is comprised predominantly of cancellous bone whereas the total body BMD is predominantly cortical bone, and the PA spine is in...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s002239900653

    authors: Aloia JF,Vaswani A,Mikhail M,Badshah M,Flaster E

    更新日期:1999-07-01 00:00:00

  • TGF-beta/extracellular matrix interactions in dentin matrix: a role in regulating sequestration and protection of bioactivity.

    abstract::TGF-beta isoforms sequestrated in dentin matrix potentially provide a reservoir of bioactive molecules that may influence cell behavior in the dentin-pulp complex following tissue injury. The association of these growth factors with dentin matrix and the influence of such associations on the bioactivity of growth fact...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-009-9248-4

    authors: Baker SM,Sugars RV,Wendel M,Smith AJ,Waddington RJ,Cooper PR,Sloan AJ

    更新日期:2009-07-01 00:00:00

  • Bone mineral density and muscle strength in young men with mental retardation (with and without Down syndrome).

    abstract::The objective of this study was to compare the bone mineral density (BMD) of men with Down syndrome (DS) to otherwise mentally retarded (MR) men and to investigate whether leg muscle strength of these patients is related to BMD. Two groups with MR (with and without DS) participated in the study, having met the followi...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s002230010035

    authors: Angelopoulou N,Matziari C,Tsimaras V,Sakadamis A,Souftas V,Mandroukas K

    更新日期:2000-03-01 00:00:00

  • Blockade of the pore-forming P2X7 receptor inhibits formation of multinucleated human osteoclasts in vitro.

    abstract::Osteoclasts are large, multinucleated, terminally differentiated cells formed by the fusion of mononuclear hemopoietic precursors. Their function is the resorption of bone, which is an essential part of the growth, modeling and remodeling of the skeleton. Though some osteoclast differentiation factors have recently be...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-002-2098-y

    authors: Gartland A,Buckley KA,Bowler WB,Gallagher JA

    更新日期:2003-10-01 00:00:00

  • A scaffold-free multicellular three-dimensional in vitro model of osteogenesis.

    abstract::In vitro models of osteogenesis are essential for investigating bone biology and the effects of pharmaceutical, chemical, and physical cues on bone formation. Osteogenesis takes place in a complex three-dimensional (3D) environment with cells from both mesenchymal and hematopoietic origins. Existing in vitro models of...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-011-9467-3

    authors: Gurkan UA,Kishore V,Condon KW,Bellido TM,Akkus O

    更新日期:2011-05-01 00:00:00

  • Effects of risedronate treatment on bone density and vertebral fracture in patients on corticosteroid therapy.

    abstract::Men and women (n = 518) receiving moderate-to-high doses of corticosteroids were enrolled in two studies with similar protocols and randomly assigned to receive either placebo or risedronate (2.5 or 5 mg) for 1 year. All patients received daily calcium supplementation (500-1000 mg), and most also received supplemental...

    journal_title:Calcified tissue international

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验

    doi:10.1007/s002230001146

    authors: Wallach S,Cohen S,Reid DM,Hughes RA,Hosking DJ,Laan RF,Doherty SM,Maricic M,Rosen C,Brown J,Barton I,Chines AA

    更新日期:2000-10-01 00:00:00

  • Influence of Teriparatide and Ibandronate on Cortical Bone in New Zealand White Rabbits: A HR-QCT Study.

    abstract::Teriparatide (TPTD) is known to increase the cortical thickness and porosity. The purpose of the present study was to determine whether switching from TPTD to ibandronate (IBN) would be useful for improving cortical bone parameters as assessed using high-resolution quantitative computed tomography (HR-QCT) analyses in...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-016-0180-0

    authors: Iwamoto J,Seki A,Nango N

    更新日期:2016-11-01 00:00:00

  • Anatomical Distribution of Ochronotic Pigment in Alkaptonuric Mice is Associated with Calcified Cartilage Chondrocytes at Osteochondral Interfaces.

    abstract::Alkaptonuria (AKU) is characterised by increased circulating homogentisic acid and deposition of ochronotic pigment in collagen-rich connective tissues (ochronosis), stiffening the tissue. This process over many years leads to a painful and severe osteoarthropathy, particularly affecting the cartilage of the spine and...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-020-00764-6

    authors: Hughes JH,Keenan CM,Sutherland H,Edwards HR,Wilson PJM,Ranganath LR,Jarvis JC,Bou-Gharios G,Gallagher JA

    更新日期:2021-02-01 00:00:00

  • Increase in tartrate-resistant acid phosphatase of bone at the early stage of ascorbic acid deficiency in the ascorbate-requiring Osteogenic Disorder Shionogi (ODS) rat.

    abstract::The effect of ascorbic acid deficiency on bone metabolism was evaluated using the ascorbate-requiring Osteogenic Disorder Shionogi (ODS) rat model. Ascorbic acid (Asc)-deficient rats gained body weight in a manner similar to Asc-supplemented rats (control) during 3 weeks, but began to lose weight during the 4th week o...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-002-2040-3

    authors: Goto A,Tsukamoto I

    更新日期:2003-08-01 00:00:00

  • The effect of fluoride on the size and morphology of apatite crystals grown from physiologic solutions.

    abstract::In adult human bone, fluoride uptake is accompanied by an increase in apatite crystal size. This increase, however, is not isotropic but is restricted primarily to growth in width and/or thickness, with no measurable change in length. In the present study, seeded growth experiments were conducted in vitro to determine...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s002239900522

    authors: Eanes ED,Hailer AW

    更新日期:1998-09-01 00:00:00

  • Diet, bone mass, and osteocalcin: a cross-sectional study.

    abstract::To determine the relationships among nutrients intake, bone mass, and bone turnover in women we have investigated these issues in a population-based, cross-sectional, observational study in one county in central Sweden. A total of 175 women aged 28-74 at entry to the study were included. Dietary assessment was made by...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF00298425

    authors: Michaëlsson K,Holmberg L,Mallmin H,Wolk A,Bergström R,Ljunghall S

    更新日期:1995-08-01 00:00:00

  • Muscle Function in Osteogenesis Imperfecta Type IV.

    abstract::Results of previous studies suggest that children and adolescents with osteogenesis imperfecta (OI) type IV have muscle force deficits. However, muscle function remains to be objectively quantified in this population. This study aimed to assess upper and lower extremity muscle function in patients with OI type IV. It ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-017-0287-y

    authors: Veilleux LN,Darsaklis VB,Montpetit K,Glorieux FH,Rauch F

    更新日期:2017-10-01 00:00:00

  • Glucose transport system in UMR-106-01 osteoblastic osteosarcoma cells: regulation by insulin.

    abstract::Insulin is a potent stimulator of collagen synthesis and other osteoblastic cell functions. In various insulin-sensitive tissues, stimulation of glucose transport and glycolytic metabolism are hallmarks of insulin action and may play a role in insulin regulation of cellular function. However, the effects of insulin on...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02556657

    authors: Ituarte EA,Halstead LR,Iida-Klein A,Ituarte HG,Hahn TJ

    更新日期:1989-07-01 00:00:00

  • Association of oxidative stress with postmenopausal osteoporosis and the effects of hydrogen peroxide on osteoclast formation in human bone marrow cell cultures.

    abstract::It has been suggested that oxidative stress is associated with the pathogenesis of osteoporosis. The objective of this study was to explore the association between a marker of oxidative stress and either bone turnover markers or bone mineral density (BMD) in postmenopausal women. In addition, the effects of oxidative ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-010-9393-9

    authors: Baek KH,Oh KW,Lee WY,Lee SS,Kim MK,Kwon HS,Rhee EJ,Han JH,Song KH,Cha BY,Lee KW,Kang MI

    更新日期:2010-09-01 00:00:00

  • Immobility as a major cause of bone remodeling in residents of a long-stay geriatric ward.

    abstract::Residents of a long-stay geriatric ward at the University Hospital Basel were included in a study to investigate the effects of hypovitaminosis D and immobility. All 91 women (mean age 82.5 years) and 92 men (mean age 78.7 years) were enrolled in the study. Measurements included bone resorption, as measured by urinary...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s002239900638

    authors: Bischoff H,Stähelin HB,Vogt P,Friderich P,Vonthein R,Tyndall A,Theiler R

    更新日期:1999-06-01 00:00:00

  • A population-based comparison of quantitative dual-energy X-ray absorptiometry with dual-photon absorptiometry of the spine and hip.

    abstract::Dual photon absorptiometry (DPA) is currently the most widely used method for noninvasive bone mineral density (BMD) measurement of the axial skeleton. Dual energy X-ray absorptiometry (DEXA) is a recently developed technique that uses an X-ray tube as a photon source; it has demonstrated several significant advantage...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02556250

    authors: Holbrook TL,Barrett-Connor E,Klauber M,Sartoris D

    更新日期:1991-11-01 00:00:00

  • Adiponectin and peak bone mass in men: a cross-sectional, population-based study.

    abstract::Adiponectin, a protein classically known to be secreted by adipocytes, is also secreted by bone-forming cells. Results of previous studies have been contradictory as to whether serum adiponectin and bone mineral density (BMD) are associated. The aim of this study was to investigate a possible association between serum...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-010-9376-x

    authors: Frost M,Abrahamsen B,Nielsen TL,Frystyk J,Flyvbjerg A,Hagen C,Andersen M,Brixen K

    更新日期:2010-07-01 00:00:00

  • Hormonal regulation of vitamin D receptor levels and osteocalcin synthesis in human osteosarcoma cells.

    abstract::1,25(OH)2D3 was found to regulate its own receptor levels via an increase in corresponding mRNA levels in human osteoblast-like osteosarcoma cells (MG-63). In addition, exposure of the cells for 24h to dexamethasone, estradiol, retinoic acid, or triiodothyronine resulted in a dose-dependent accumulation of hVDR mRNA. ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02555101

    authors: Mäenpää P,Mahonen A,Pirskanen A

    更新日期:1991-01-01 00:00:00

  • Ultrastructural immunolocalization of cartilage oligomeric matrix protein (COMP) in porcine growth cartilage.

    abstract::Cartilage oligomeric matrix protein (COMP) is a macromolecule of yet unknown function with restricted distribution among tissues. In the present study, the ultrastructural localization of COMP in porcine immature joint cartilage and growth plate cartilage was semiquantitatively delineated. Tissues were fixed in a mixt...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s002239900278

    authors: Ekman S,Reinholt FP,Hultenby K,Heinegârd D

    更新日期:1997-06-01 00:00:00

  • Dkk1 KO Mice Treated with Sclerostin Antibody Have Additional Increases in Bone Volume.

    abstract::Dickkopf-1 (DKK1) and sclerostin are antagonists of the Wnt/β-catenin pathway and decreased expression of either results in increased bone formation and mass. As both affect the same signaling pathway, we aimed to elucidate the redundancy and/or compensation of sclerostin and DKK1. Weekly sclerostin antibody (Scl-Ab) ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-018-0420-6

    authors: Morse A,Cheng TL,Schindeler A,McDonald MM,Mohanty ST,Kneissel M,Kramer I,Little DG

    更新日期:2018-09-01 00:00:00

  • Dietary patterns in relation to bone mineral density among menopausal Iranian women.

    abstract::The association of dietary patterns and bone health is not yet well known, and findings from the rare previous studies conducted on this issue are contradictory. We assessed the dietary patterns in relation to bone mineral density (BMD) in a sample of menopausal Iranian women. In this cross-sectional study, 160 menopa...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-012-9608-3

    authors: Karamati M,Jessri M,Shariati-Bafghi SE,Rashidkhani B

    更新日期:2012-07-01 00:00:00

  • Evaluation of partially demineralized osteoporotic cancellous bone matrix combined with human bone marrow stromal cells for tissue engineering: an in vitro and in vivo study.

    abstract::Allogenous demineralized bone matrix (DBM) represents a potential scaffold for bone tissue engineering due to its close relation in structure and function with autologous bone, but its supply is often restricted by donor availability. Thus, an expanded source of human bone is needed. The aim of this study was to evalu...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-008-9159-9

    authors: Liu G,Sun J,Li Y,Zhou H,Cui L,Liu W,Cao Y

    更新日期:2008-09-01 00:00:00

  • Influence of birth weight on calcaneal bone stiffness in Belgian preadolescent children.

    abstract::The aim of this study was to investigate the relation between birth weight and calcaneal bone stiffness in a large sample of Belgian, healthy, preadolescent children. Participants were 827 children (3.6-11.2 years, 51.6 % boys) from the Belgian cohort of the IDEFICS study. Birth weight was obtained using a parental qu...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-012-9636-z

    authors: Van den Bussche K,Michels N,Gracia-Marco L,Herrmann D,Eiben G,De Henauw S,Sioen I

    更新日期:2012-10-01 00:00:00

  • Demonstration of calmodulin-sensitive calcium translocation by isolated osteoclast plasma membrane vesicles.

    abstract::Plasma membrane vesicles were prepared from chicken osteoclasts, and active calcium transport was demonstrated in a spectrofluorimetric assay using the fluorescent calcium concentration indicator, fura-2. Transport activity was inhibited by quercetin (10 microM), sodium vanadate (10 microM), and the anticalmodulin age...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF00334493

    authors: Bekker PJ,Gay CV

    更新日期:1992-10-01 00:00:00

  • Centrifugal isolation of bone marrow from bone: an improved method for the recovery and quantitation of bone marrow osteoprogenitor cells from rat tibiae and femurae.

    abstract::SUMMARY. The high variation often observed in the ex vivo fibroblastic-colony forming unit (CFU-f) assay is likely to be due to both biological and experimental variation. To determine whether we could improve experimental methods we developed an alternative method of bone marrow cell (BMC) isolation employing a centr...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s002239900723

    authors: Dobson KR,Reading L,Haberey M,Marine X,Scutt A

    更新日期:1999-11-01 00:00:00

  • Blood:bone disequilibrium. VI. Studies of the solubility characteristics of brushite: apatite mixtures and their stabilization by noncollagenous proteins of bone.

    abstract::Recent evidence of the occurrence of brushite in newly formed bone mineral prompted a study of the solubility properties of brushite:apatite mixtures under physiological conditions and the influence on them of pH, lactate, pyruvate, and, particularly, noncollagenous bone proteins (NCBPs). Brushite alone was surprising...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02411226

    authors: Neuman WF,Neuman MW,Diamond AG,Menanteau J,Gibbons WS

    更新日期:1982-03-01 00:00:00

  • Age-dependent effect of oral glucocorticoids on markers of bone resorption in patients with acute asthma.

    abstract::It is generally accepted that bone formation is depressed during corticosteroid treatment, but the effects of glucocorticoids on bone resorption are less well characterized. We have investigated the effects of short-term treatment with high-dose oral glucocorticoids on biochemical markers of bone turnover in 20 consec...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s002239900482

    authors: Bornefalk E,Dahlén I,Michaëlsson K,Ljunggren,Ljunghall S

    更新日期:1998-07-01 00:00:00

  • Cathepsin K preferentially solubilizes matured bone matrix.

    abstract::Bone collagen undergoes a series of enzymatic and nonenzymatic posttranslational modifications with maturation. The aim of this study was to analyze the collagenolytic efficiency of cathepsin K in relation to the extent of bone collagen age. Bone collagen posttranslational maturation was induced in vitro by preincubat...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-012-9604-7

    authors: Borel O,Gineyts E,Bertholon C,Garnero P

    更新日期:2012-07-01 00:00:00