Abstract:
:Bone is a dynamic tissue that is able to sense and adapt to mechanical stimuli by modulating its mass, geometry, and structure. Bone marrow stromal cells (BMSCs) are known to play an integral part in bone formation by providing an osteoprogenitor cell source capable of differentiating into mature osteoblasts in response to mechanical stresses. Characteristics of the in vivo bone environment including the three dimensional (3-D) lacunocanalicular structure and extracellular matrix composition have previously been shown to play major roles in influencing mechanotransduction processes within bone cells. To more accurately model this phenomenon in vitro, we cultured human BMSCs on 3-D, partially demineralized bone scaffolds in the presence of four-point bending loads within a novel bioreactor. The effect of mechanical loading and dexamethasone concentration on BMSC osteogenic differentiation and mineralized matrix production was studied for 8 and 16 days of culture. Mechanical stimulation after 16 days with 10 nM dexamethasone promoted osteogenic differentiation of BMSCs by significantly elevating alkaline phosphatase activity as well as alkaline phosphatase and osteopontin transcript levels over static controls. Mineralized matrix production also increased under these culture conditions. Dexamethasone concentration had a dramatic effect on the ability of mechanical stimulation to modulate these phenotypic and genotypic responses. These results provide increased insight into the role of mechanical stimulation on osteogenic differentiation of human BMSCs in vitro and may lead to improved strategies in bone tissue engineering.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Mauney JR,Sjostorm S,Blumberg J,Horan R,O'Leary JP,Vunjak-Novakovic G,Volloch V,Kaplan DLdoi
10.1007/s00223-003-0104-7subject
Has Abstractpub_date
2004-05-01 00:00:00pages
458-68issue
5eissn
0171-967Xissn
1432-0827journal_volume
74pub_type
杂志文章abstract::In the past years, several epidemiological and clinical observations have underlined the importance of genetics in the pathogenesis of both female and male osteoporosis. It has been estimated that from 50 to 80% of the inter-individual variability in bone mass is genetically determined. In rare instances, osteoporosis...
journal_title:Calcified tissue international
pub_type: 杂志文章,评审
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abstract::Prosected vertebrae and phalanges from 32 cadavera were examined using noninvasive methods commonly used for assessment of osteoporosis. Bone measures were then compared with a strength variable obtained by mechanically crushing or breaking the bones. All of the phalangeal density assessment techniques were found to b...
journal_title:Calcified tissue international
pub_type: 杂志文章
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
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更新日期:2000-10-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002239900638
更新日期:1999-06-01 00:00:00
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journal_title:Calcified tissue international
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