The use of quantitative scintigraphy in the measurement of portal-systemic shunting in rats.

Abstract:

:Portal-systemic shunting was studied in 54 portal hypertensive rats both in vivo and in vitro using radioactive microspheres. The animals underwent partial portal vein ligation around needles of varying diameter to produce a wide range of shunting. Two to four weeks later, quantitative lung-liver scintigraphic and whole body images were obtained in vivo following ileocolic vein injection with 99mTc-MAA. After sacrifice, the lung and liver activities were determined by the gamma camera, a dose calibrator, and a well counter. Portal-systemic shunting ranged from 0.1-97.6%. When shunting was compared in vivo and in vitro, an excellent correlation was found (r = 0.99, p < 0.001). A subgroup of 24 animals had consecutive injections of 99mTc-MAA and 51Cr-labeled 15 microns microspheres, which, although different in size, yielded similar results (r = 0.89, p < 0.001). We conclude that in small laboratory animals a wide range of shunting can be measured accurately in vivo by quantitative scintigraphy.

journal_name

Ann Nucl Med

authors

Stauber RE,Mochizuki T,Van Thiel DH,Tauxe WN

doi

10.1007/BF03164656

subject

Has Abstract

pub_date

1992-11-01 00:00:00

pages

209-14

issue

4

eissn

0914-7187

issn

1864-6433

journal_volume

6

pub_type

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