TMX, a human transmembrane oxidoreductase of the thioredoxin family: the possible role in disulfide-linked protein folding in the endoplasmic reticulum.

abstract：:We have studied the effectiveness of polyprenyl-P-mannose and polyprenol-P-glucose as donor substrates for the dolichyl-P-mannose:Man5(GlcNAc)2-PP-dolichol mannosyltransferase and the dolichyl-P-glucose:Man9(GlcNAc)2-PP-dolichol glucosyltransferase, respectively. The polyprenol moiety differs from dolichol only in the...

journal_title：Archives of biochemistry and biophysics

authors： D'Souza-Schorey C,McLachlan KR,Krag SS,Elbein AD

更新日期：1994-02-01 00:00:00

abstract：:The multifunctional tyrosine N-hydroxylase, cytochrome P450TYR (CYP79), from Sorghum bicolor catalyzing the conversion of tyrosine to p-hydroxyphenyl-acetaldoxime in the biosynthesis of the cyanogenic glucoside dhurrin, has been expressed in Escherichia coli using the isopropyl-beta-D-thiogalactopyranoside-inducible v...

journal_title：Archives of biochemistry and biophysics

authors： Halkier BA,Nielsen HL,Koch B,Møller BL

更新日期：1995-10-01 00:00:00

abstract：:Human plasma glutathione peroxidase was purified to homogeneity and partially sequenced. Overlapping peptide fragments from three endopeptidase digests permitted the determination of one sequence of 32 contiguous amino acids and one sequence of 23 contiguous amino acids. Five additional unique peptide sequences withou...

journal_title：Archives of biochemistry and biophysics

authors： Esworthy RS,Chu FF,Paxton RJ,Akman S,Doroshow JH

更新日期：1991-05-01 00:00:00

abstract：:We have found for the first time that a chromosomal gene, mlr6807, in Mesorhizobium loti encodes a new tetrameric pyridoxal 4-dehydrogenase (PLDH). The recombinant enzyme expressed in Escherichia coli cells was homogenously purified and characterized. The enzyme consisted of four subunits each with a molecular weight ...

journal_title：Archives of biochemistry and biophysics

authors： Yokochi N,Nishimura S,Yoshikane Y,Ohnishi K,Yagi T

更新日期：2006-08-01 00:00:00

abstract：:Cadmium is toxic and carcinogenic to humans and animals. The testis and lung are the target organs for cadmium carcinogenesis. Heat shock proteins (HSPs) as well as metallothionein (MT) and glutathione (GSH) play an important role in protection against its toxicity. HSP32, also known as heme oxygenase-1, is a 32-kDa p...

journal_title：Archives of biochemistry and biophysics

authors： Abe T,Yamamoto O,Gotoh S,Yan Y,Todaka N,Higashi K

更新日期：2000-10-01 00:00:00

abstract：:Low-temperature plasma is useful for the care of wounded skin. It accelerates wound healing. However, the mechanism of this effect has not been fully elucidated yet. Galectin-1 is reported to accelerate wound healing via the Smad signaling pathway. In the present study to clarify whether or not galectins were expresse...

journal_title：Archives of biochemistry and biophysics

authors： Akimoto Y,Ikehara S,Yamaguchi T,Kim J,Kawakami H,Shimizu N,Hori M,Sakakita H,Ikehara Y

更新日期：2016-09-01 00:00:00

abstract：:Glycosaminoglycan (GAG)-protein interactions regulate a myriad of physiologic and pathologic processes, yet an understanding of how these molecules interact is lacking. The role of the pattern and spacing of basic amino acids (arginine (R) and lysine (K)) in heparin binding sites was investigated using peptide analogs...

journal_title：Archives of biochemistry and biophysics

authors： Fromm JR,Hileman RE,Caldwell EE,Weiler JM,Linhardt RJ

更新日期：1997-07-01 00:00:00

abstract：:Concentrations of GDP, which are expected to bind to the catalytic site and inhibit the autophosphorylation of succinyl-CoA synthetase (SCS) when NTP is used as a substrate, were found to increase the level of phosphoenzyme formed. The ability of GDP to do so is dependent upon the presence of a protein distinct from S...

journal_title：Archives of biochemistry and biophysics

authors： Birney M,Um H,Klein C

更新日期：1997-11-01 00:00:00

abstract：:Phosphatidylserine (PS) is predominantly confined to the inner leaflet of plasma membrane in cells, but it is externalized on the cell surface during apoptosis. This externalized PS is required for effective phagocytosis of apoptotic cells by macrophages. Because PS trans-bilayer asymmetry is not absolute in different...

journal_title：Archives of biochemistry and biophysics

authors： Borisenko GG,Matsura T,Liu SX,Tyurin VA,Jianfei J,Serinkan FB,Kagan VE

更新日期：2003-05-01 00:00:00

abstract：:The Metabotropic glutamate receptor 2 (mGluR2) is involved in several neurological and psychiatric disorders and is an attractive drug target. It is believed to form a strict dimer and the dimeric assembly is necessary for glutamate induced activation. Although many studies have focused on glutamate induced conformati...

journal_title：Archives of biochemistry and biophysics

authors： Singh DR,Pandey K,Mishra AK,Pandey P,Vivcharuk V

更新日期：2021-01-15 00:00:00

abstract：:The aim of the present study was to further understand how changes in membrane organization can lead to higher rates of lipid oxidation. We previously demonstrated that Al(3+), Sc(3+), Ga(3+), Be(2+), Y(3+), and La(3+) promote lipid packing and lateral phase separation. Using the probe Laurdan, we evaluated in liposom...

journal_title：Archives of biochemistry and biophysics

authors： Verstraeten SV,Oteiza PI

更新日期：2000-03-15 00:00:00

abstract：:A green manganese desferrioxamine complex is rapidly formed at room temperature upon stirring freshly precipitated manganese dioxide in a solution of the ligand. Spectral studies and low-temperature ESR indicate that this compound, which has been previously described as a manganese(III) complex, is better characterize...

journal_title：Archives of biochemistry and biophysics

authors： Rush JD,Maskos Z,Koppenol WH

更新日期：1991-08-15 00:00:00

abstract：:Some compounds are known to induce solute-nonselective permeability of the inner mitochondrial membrane (IMM) in Ca2+-loaded mitochondria. Existing data suggest that this process, following the opening of a mitochondrial permeability transition pore, is preceded by different solute-selective permeable states of IMM. A...

journal_title：Archives of biochemistry and biophysics

authors： Balakirev MY,Zimmer G

更新日期：1998-08-01 00:00:00

abstract：:The G1 glycoprotein of Punta Toro virus, a member of the bunyavirus family, accumulates in the Golgi complex when it is expressed from cloned cDNA. We previously reported that the information necessary for Golgi retention of the G1 protein is located within the transmembrane domain and a portion of the cytoplasmic dom...

journal_title：Archives of biochemistry and biophysics

authors： Matsuoka Y,Chen SY,Holland CE,Compans RW

更新日期：1996-12-01 00:00:00

abstract：:(6R)-5,6,7,8-Tetrahydro-L-biopterin (R-THBP) is a cofactor not only for aromatic amino acid hydroxylases in mammalian tissues but also for nitric oxide synthase (NOS) induced by endotoxins or cytokines in some kinds of cells. Recently it has been reported that nitric oxide (NO) has biological activity in endothelium a...

journal_title：Archives of biochemistry and biophysics

authors： Nunokawa Y,Ishihara T,Kanai T,Noguchi T

更新日期：1992-11-01 00:00:00

abstract：:Hemorrhagic toxin f (HT-f) was isolated from Crotalus atrox (Western Diamondback Rattlesnake) venom by a five-step purification procedure. Homogeneity was established by the formation of a single band in acrylamide gel electrophoresis, isoelectric focusing, and sodium dodecyl sulfate (SDS)-electrophoresis. HT-f has a ...

journal_title：Archives of biochemistry and biophysics

authors： Nikai T,Mori N,Kishida M,Sugihara H,Tu AT

更新日期：1984-06-01 00:00:00

abstract：:Glycine residues are recognized as important structural determinants in nucleotide-binding domains of many enzymes. The functional significance of seven glycine residues invariant in all 22 eNTPDase sequences was therefore examined. Glycine-to-alanine mutants of eNTPDase3 were analyzed for nucleotidase activities and ...

journal_title：Archives of biochemistry and biophysics

authors： Kirley TL,Yang F,Ivanenkov VV

更新日期：2001-11-01 00:00:00

abstract：:Two cDNAs encoding taxoid-O-acetyl transferases (TAX 9 and TAX 14) were obtained from a previously isolated family of Taxus acyl/aroyl transferase cDNA clones. The recombinant enzymes catalyze the acetylation of taxadien-5alpha,13alpha-diacetoxy-9alpha,10beta-diol to generate taxadien-5alpha,10beta,13alpha-tri-acetoxy...

journal_title：Archives of biochemistry and biophysics

authors： Hampel D,Mau CJ,Croteau RB

更新日期：2009-07-15 00:00:00

abstract：:This paper describes the effect of an ionic (sodium dodecyl sulfate; SDS) and a nonionic detergent (Triton X-100) on the substrate and enzyme components of the lecithin: cholesterol acyltransferase (LCAT) reaction. When the enzyme sources (purified or partially purified) or the respective substrates [high-density lipo...

journal_title：Archives of biochemistry and biophysics

authors： Park MC,McConathy WJ,Lacko AG

更新日期：1984-08-15 00:00:00

abstract：:The dicarbonyl compounds, phenylgloxyl and 2,3-butanedione were used to demonstrate the presence of an essential arginine residue in the mechanism of the red beet (Beta vulgaris L.) plasma membrane ATPase. Treatment of the red beet ATPase with either of these reagents resulted in an inhibition of ATP hydrolytic activi...

journal_title：Archives of biochemistry and biophysics

authors： Gildensoph LH,Briskin DP

更新日期：1989-05-15 00:00:00

abstract：:The decomposition of S-nitrosoglutathione (GSNO) in the presence of Cu2+ and glutathione (GSH) was studied by stopped-flow/rapid-scan spectroscopy. Reduction of Cu2+ by GSH and subsequent formation of a GS-*Cu+ complex occurred within 200 ms, with the amount of complex formed depending on the GSH-to-Cu2+ ratio. The ra...

journal_title：Archives of biochemistry and biophysics

authors： Gorren AC,Schrammel Astrid,Schmidt K,Mayer B

更新日期：1996-06-15 00:00:00

abstract：:Aldehyde oxidase (AO) and xanthine oxidase (XO) are cytosolic enzymes that have been involved in some pathological conditions and play an important role in the biotransformation of drugs and xenobiotics. The increasing interest in these enzymes demands for a simple and rapid procedure for their purification. This pape...

journal_title：Archives of biochemistry and biophysics

authors： Maia L,Mira L

更新日期：2002-04-01 00:00:00

abstract：:Calcium ion is an uncompetitive inhibitor of the inorganic pyrophosphatases of bovine heart and rat liver mitochondria with respect to substrate MgPPi at pH 8.5 and a non-competitive inhibitor of the former enzyme at pH 7.2. The concentration of Ca2+ required to decrease the maximal velocities for both enzymes at pH 8...

journal_title：Archives of biochemistry and biophysics

authors： Baykov AA,Volk SE,Unguryte A

更新日期：1989-09-01 00:00:00

abstract：:CYP119 from Sulfolobus solfataricus is the first well-characterized thermophilic cytochrome P450 enzyme. The endogenous substrate for this enzyme is not known but it hydroxylates lauric acid in a reaction supported by surrogate mesophilic electron donors. However, reconstitution of a high-temperature catalytic system ...

journal_title：Archives of biochemistry and biophysics

authors： Puchkaev AV,Ortiz de Montellano PR

更新日期：2005-02-01 00:00:00

abstract：:Electrophoretically homogeneous preparations of catalytic subunit (C) of cAMP-dependent protein kinase isolated according to two different procedures from holoenzyme type I and type II from rabbit and from holoenzyme type II from rat skeletal muscle and from bovine cardiac muscle can be separated on carboxymethyl cell...

journal_title：Archives of biochemistry and biophysics

authors： Kinzel V,Hotz A,König N,Gagelmann M,Pyerin W,Reed J,Kübler D,Hofmann F,Obst C,Gensheimer HP

更新日期：1987-03-01 00:00:00

abstract：:Effects of free fatty acids on parameters of (Na+,K+)-ATPase regulation related to enzyme conformation were examined. Sensitivity to inhibition by free fatty acid increased as the number of double bonds increased. Free fatty acids reduced affinity for K+ or Na+ at their regulatory sites without altering apparent K+ af...

journal_title：Archives of biochemistry and biophysics

authors： Swann AC

更新日期：1984-09-01 00:00:00

abstract：:A kinetic quantification of the lipoperoxyl radical-scavenging activity of all-trans-retinol has been carried out in homogeneous solution, when radicals were produced from the oxidation of methyl linoleate in methanol, initiated by the lipid-soluble 2,2'-azobis (2,4-dimethylvaleronitrile) (AMVN) as well as in a soybea...

journal_title：Archives of biochemistry and biophysics

authors： Tesoriere L,Ciaccio M,Bongiorno A,Riccio A,Pintaudi AM,Livrea MA

更新日期：1993-11-15 00:00:00

abstract：:Considerable metabolic energy is expended in ensuring that membranes possess a characteristic fatty acid composition. The nature of the specific requirement of the retina for high levels of docosahexaenoic acid (DHA) is as yet undefined. Previous work has speculated that DHA is required to maintain the fluid nature an...

journal_title：Archives of biochemistry and biophysics

authors： Treen M,Uauy RD,Jameson DM,Thomas VL,Hoffman DR

更新日期：1992-05-01 00:00:00

abstract：:Aldehyde oxidase (EC 1.2.3.1) plays a role in the oxidation of aromatic heterocyclic compounds ingested by some higher vertebrates. To better understand this function, the specificity of the rabbit liver enzyme toward purines and their analogs was quantitatively studied. The chemical nature of the 6-substituent of pur...

journal_title：Archives of biochemistry and biophysics

authors： Hall WW,Krenitsky TA

更新日期：1986-11-15 00:00:00

abstract：:An N-acetyl-D-galactosamine-specific lectin has been isolated from the two seed forms of the hog peanut (Amphicarpaea bracteata) using an affinity support containing the synthetic type A blood group trisaccharide alpha-D-GalNAc-(1,3)-[alpha-L-Fuc-(1,2)]-beta-D-Gal (Synsorb A). The affinity-purified lectin appears to b...

journal_title：Archives of biochemistry and biophysics

authors： Maliarik MJ,Roberts DD,Goldstein IJ

更新日期：1987-05-15 00:00:00