Abstract:
:A PCR-RFLP based method was developed to diagnose and identify the Leishmania species causing American cutaneous leishmaniasis (ACL) in a panel of clinical samples obtained from an endemic region of Brazil. The comparison of the results obtained by PCR-RFLP and PCR-hybridization in the identification of Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis were highly concordant (kappa=91.5%). The PCR-RFLP method was reliable, fast and easy to conduct on biopsies and presents potential value of utmost importance for the diagnosis and identification of Leishmania in clinical specimens, infected reservoirs and vectors.
journal_name
Acta Tropjournal_title
Acta tropicaauthors
Volpini AC,Passos VM,Oliveira GC,Romanha AJdoi
10.1016/j.actatropica.2003.10.008subject
Has Abstractpub_date
2004-03-01 00:00:00pages
31-7issue
1eissn
0001-706Xissn
1873-6254pii
S0001706X03002808journal_volume
90pub_type
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