PCR-RFLP to identify Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis causing American cutaneous leishmaniasis.

Abstract:

:A PCR-RFLP based method was developed to diagnose and identify the Leishmania species causing American cutaneous leishmaniasis (ACL) in a panel of clinical samples obtained from an endemic region of Brazil. The comparison of the results obtained by PCR-RFLP and PCR-hybridization in the identification of Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis were highly concordant (kappa=91.5%). The PCR-RFLP method was reliable, fast and easy to conduct on biopsies and presents potential value of utmost importance for the diagnosis and identification of Leishmania in clinical specimens, infected reservoirs and vectors.

journal_name

Acta Trop

journal_title

Acta tropica

authors

Volpini AC,Passos VM,Oliveira GC,Romanha AJ

doi

10.1016/j.actatropica.2003.10.008

subject

Has Abstract

pub_date

2004-03-01 00:00:00

pages

31-7

issue

1

eissn

0001-706X

issn

1873-6254

pii

S0001706X03002808

journal_volume

90

pub_type

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