Abstract:
BACKGROUND AND AIMS:Pancreatic stellate cells (PSCs) play a key role in the development of pancreatic fibrosis. The molecular mechanisms underlying their activation in response to profibrogenic mediators, however, are largely unknown. Extending previous studies on the transcriptional regulation of PSC activation, we have now focused on the involvement of activator protein (AP)-1. MATERIALS AND METHODS:Using cultured rat PSCs, phenotypic transition of PSCs towards activated myofibroblasts was monitored by an immunoblot analysis of alpha-smooth muscle actin (alpha-SMA) expression. Transcription factor activation profiles were studied by electrophoretic mobility shift assays. DNA synthesis in PSCs was assessed through the quantification of 5-bromo-2'-deoxyuridine incorporation. RESULTS:Activated AP-1 complexes were detectable already before high levels of alpha-SMA were expressed. Maximal DNA binding activity of AP-1, as well as of NF-kappaB, was observed early in the course of PSC culture, while the strongest activation of STAT3 was observed much later. A detailed analysis of AP-1 complex composition revealed that phenotypic transition of PSCs towards myofibroblasts was accompanied by an increase of the JunD content relative to the one of JunB. Studies on the role of JunB and JunD in PSC activation indicated an inhibition of platelet-derived growth factor-induced DNA synthesis by antisense oligonucleotides to JunB but not JunD. CONCLUSIONS:The results of this study implicate AP-1 in PSC activation and suggest distinct roles of individual Jun proteins in the regulation of PSC function. In further studies, it should be analyzed whether signaling pathways involved in PSC activation might be suitable targets for antifibrotic therapies.
journal_name
Int J Colorectal Disjournal_title
International journal of colorectal diseaseauthors
Fitzner B,Sparmann G,Emmrich J,Liebe S,Jaster Rdoi
10.1007/s00384-003-0565-1subject
Has Abstractpub_date
2004-09-01 00:00:00pages
414-20issue
5eissn
0179-1958issn
1432-1262journal_volume
19pub_type
杂志文章abstract::440 patients were prospectively enrolled in a randomized, multicenter trial to compare 4 types of manual (84 interrupted end-to-end, 77 continuous end-to-end, 82 interrupted end-to-side, and 91 continuous end-to-side) (polyglycolic derived suture) and 1 type of stapled (106 side-to-side with GIA+TA devices) ileocoloni...
journal_title:International journal of colorectal disease
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1007/BF00341273
更新日期:1993-03-01 00:00:00
abstract:PURPOSE:Since colorectal endoscopic submucosal dissection (ESD) requires higher-level skills than endoscopic mucosal resection (EMR), it is recommended to acquire sufficient experience in gastric ESD prior to attempting colorectal ESD. We evaluated the ability of experienced endoscopists with limited experience in gast...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-015-2334-3
更新日期:2015-12-01 00:00:00
abstract:PURPOSE:It is controversial whether patients fare better with conservative or surgical treatment in certain stages of acute diverticulitis (AD), in particular when phlegmonous inflammation or covered micro- or macro-perforation are present. The aim of this study was to determine long-term quality of life (QoL) for AD p...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-018-2969-y
更新日期:2018-03-01 00:00:00
abstract:AIM:Evidence is lacking whether an abscess associated with anastomotic leak after an ileal pouch-anal anastomosis (IPAA) should be drained by transanal or CT-guided drainage. Therefore, the aim of this study was to compare outcomes after the two techniques for drainage. METHOD:Patients who underwent IPAA (1984-2009) a...
journal_title:International journal of colorectal disease
pub_type: 临床试验,杂志文章
doi:10.1007/s00384-011-1272-y
更新日期:2011-11-01 00:00:00
abstract::Twenty-two patients with colorectal cancer and synchronous unresectable hepatic metastases were treated by resection the primary tumour with concurrent insertion of an Infusaid infusaport system for regional chemoperfusion (hepatic arterial 20, portal venous 2). Four patients in addition had cryotherapy the liver meta...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/BF00418854
更新日期:1996-01-01 00:00:00
abstract:PURPOSE:Rectal advancement flap is an accepted approach for treating complex fistula-in-ano. However, a diversity of technical modifications have been described. The aim of this study was to evaluate recurrence and fecal continence rates after performing rectal advancement flaps depending upon flap thickness (full-thic...
journal_title:International journal of colorectal disease
pub_type: 杂志文章,meta分析,评审
doi:10.1007/s00384-017-2779-7
更新日期:2017-05-01 00:00:00
abstract::The authors of the published version of this article inadvertently omitted the table note of the Table 2 under the Appendix section. The complete Table 2 is shown in this article]. ...
journal_title:International journal of colorectal disease
pub_type: 已发布勘误
doi:10.1007/s00384-020-03572-4
更新日期:2020-04-20 00:00:00
abstract:PURPOSE:Inflammatory bowel disease has been associated with neurological symptoms including restless legs syndrome. Here, we investigated the impact of restless legs syndrome in patients with inflammatory bowel disease on sleep, fatigue, mood, cognition, and quality of life. METHODS:Two groups of inflammatory bowel di...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-016-2681-8
更新日期:2017-01-01 00:00:00
abstract:PURPOSE:Ambulatory laparoscopic appendectomy has gained popularity due to the improved understanding of patient selection criteria, the application of enhanced recovery pathways, and the potential for improving healthcare resource utilization. The aim of the review was to compare the morbidity and readmission rates bet...
journal_title:International journal of colorectal disease
pub_type: 杂志文章,meta分析
doi:10.1007/s00384-019-03341-y
更新日期:2019-08-01 00:00:00
abstract:BACKGROUND:There is no general consensus regarding the ideal timing of surgery in patients with refractory ulcerative colitis (UC). Decision-making and timing of restorative proctocolectomy with ileal pouch-anal anastomosis (IPAA) is influenced by treating physicians and patients themselves. The aim of this study was t...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-018-3129-0
更新日期:2018-10-01 00:00:00
abstract::Pouchitis is a well recognized entity but its aetiology is still controversial. The aim of this study is to determine if gastric acidity has a role in the physiopathology of this problem. In a series of 70 consecutive patients with pelvic reservoir (65 mucosal ulcerative colitis (UC), 5 familial adenomatous polyposis ...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/BF00300403
更新日期:1990-08-01 00:00:00
abstract::Disruption of the anal sphincter results from obstetric injury, ano-rectal operations or external trauma. Obstetric or surgical division of the sphincteric mechanism may not be immediately apparent and the clinical presentation of incontinence may occur several years later [1, 2]. Reconstruction of the sphincter using...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/BF00699424
更新日期:1994-05-01 00:00:00
abstract:BACKGROUND:Human phosphatases of regenerating liver (PRLs) can induce cell growth, differentiation, and malignant transformation. In this study, we used specific polyclonal antibodies against PRLs to investigate their expression in colonic adenocarcinomas and its correlation with patient gender, age, tumor differentiat...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-007-0303-1
更新日期:2007-10-01 00:00:00
abstract:PURPOSE:The ideal timing for patients with colorectal cancer to undergo surgery for resectable synchronous liver metastases remains under debate. We describe a new sequential approach using laparoscopic/robotic surgery for the treatment of synchronous liver metastases. METHODS:A 73-year-old man presented with sigmoid ...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-010-1000-z
更新日期:2010-12-01 00:00:00
abstract:PURPOSE:For rectal neuroendocrine tumors (NETs) ≤ 10 mm, endoscopic resection is a standard treatment. However, there is no consensus whether additional surgery should be performed for patients at risk of lymph node metastasis (LNM) after endoscopic resection. The purpose of this study was to analyze the results of end...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-020-03826-1
更新日期:2021-01-02 00:00:00
abstract:PURPOSE:Colonic manometry (CM) can be of additive value in the diagnostic workup of colonic motility in chronic constipated patients. However, it is claimed that colonic motor disturbances occur in normal-transit constipation (NTC) and slow-transit (STC) constipation, as measured using a radio-opaque marker study, and ...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-019-03376-1
更新日期:2019-10-01 00:00:00
abstract:PURPOSE:Kras mutations are negative predictors of anti-EGFR therapy, occurring in 40% of colorectal carcinomas (CRCs). Point substitutions in codon 12 or 13 are the most frequent mutations in Kras, but multiple mutations (MMs) in other codons can also develop. Few data exist on MMs with regard to their frequency and th...
journal_title:International journal of colorectal disease
pub_type: 杂志文章,评审
doi:10.1007/s00384-011-1238-0
更新日期:2011-10-01 00:00:00
abstract:BACKGROUND:Gastrointestinal stromal tumors (GISTs) of the rectum are rarely found, and radical surgery such as abdominoperineal resection would be necessary for large rectal GIST. On the other hand, therapy for GIST has changed significantly with the use of imatinib. Neoadjuvant imatinib therapy may reduce tumor size a...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-013-1769-7
更新日期:2014-01-01 00:00:00
abstract:PURPOSE:Our previous study reported that carcinoembryonic antigen (CEA) levels in peritoneal fluid were significantly correlated with the prevalence of peritoneal carcinomatosis (PC) in colorectal cancer (CRC). The purpose of this study was a long-term follow up of the author's previous study, as well as the identifica...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-019-03448-2
更新日期:2020-01-01 00:00:00
abstract:PURPOSE:Lymph node (LN) yield is a critical component of colon cancer staging and is often a surrogate for quality assessment in surgery. We investigated the impact of pathologists' training on LN harvest. METHODS:This is a retrospective review on 137 patients undergoing elective colectomy for adenocarcinoma at a sing...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-011-1240-6
更新日期:2011-09-01 00:00:00
abstract:BACKGROUND AND AIMS:MMP-7, a member of the matrix metalloproteinase family, is believed to play a significant role in the growth and proliferation of colon cancer cells. The aim of this study was to evaluate MMP-7 gene expression in comparison with MMP-1, MMP-3, and MMP-13 in patients with resectable rectal and colon c...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-004-0592-6
更新日期:2004-11-01 00:00:00
abstract:BACKGROUND AND AIMS:A 5-bp insertion-deletion (indel) polymorphism in the promoter of interferon regulatory factor 5 (IRF5) has been associated with inflammatory bowel diseases (IBD). This polymorphism generates an additional binding site for the transcription factor SP1 and has been shown to augment the expression of ...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-010-0874-0
更新日期:2010-05-01 00:00:00
abstract:PURPOSE:Cancerous involvement of a ureter is sometimes encountered in pelvic surgery for malignancy. We usually perform transureteroureterostomy (TUU) in cases of unilateral lower ureteral cancerous involvement. We report the outcomes in patients treated with TUU in our institute. METHODS:We retrospectively reviewed t...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-016-2672-9
更新日期:2017-01-01 00:00:00
abstract:PURPOSE:This study investigated whether covering the colonic anastomoses with amniotic membrane (AM) protects the anastomotic healing from the adverse effects of immediate 5-fluorouracil (5-FU) administration. METHODS:One hundred twenty wistar albino rats were randomized to one of four groups (I-IV, 30 rats in each) a...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-009-0833-9
更新日期:2010-02-01 00:00:00
abstract:AIMS:This study assesses the value of endorectal ultrasound (ERUS)-directed biopsy in detecting local recurrence of rectal cancer. METHODS:We reviewed the records of patients undergoing ERUS by a single surgeon for surveillance after treatment of rectal adenocarcinoma. Lesions suggestive of local recurrence underwent ...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-005-0785-7
更新日期:2006-04-01 00:00:00
abstract:BACKGROUND:The debate over sentinel lymph node mapping (SLNM) and focused pathologic examination to detect micrometastases in patients with colorectal cancer (CRC) continues. We present in this paper our experience with SLNM for CRCs to improve staging. In addition, we have detailed the mapping procedure on an anatomic...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-006-0132-7
更新日期:2007-02-01 00:00:00
abstract:BACKGROUND:Postoperative ileus is a significant clinical challenge lacking effective management strategies. Ghrelin-receptor stimulation has promotility effects in the upper and lower gastrointestinal tract. OBJECTIVE:This proof-of-concept, phase 2, randomized study evaluated the safety and efficacy of the ghrelin-rec...
journal_title:International journal of colorectal disease
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00384-014-2030-8
更新日期:2014-12-01 00:00:00
abstract:BACKGROUND AND AIMS:Anal abscess is a frequent acute proctological disorder and whether the underlying fistula should be treated at the same time when the abscess is drained remains controversial. We examined indications for drainage alone versus drainage plus fistulotomy in terms of recurrence and continence. PATIENT...
journal_title:International journal of colorectal disease
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s00384-002-0429-0
更新日期:2003-03-01 00:00:00
abstract:PURPOSE:Pelvic radiotherapy may lead to changes of anorectal function resulting in incontinence-related complaints. The aim of this study was to systematically review objective findings of late anorectal physiology and mucosal appearance after irradiation for prostate cancer. METHODS:MEDLINE, EMBASE, and the Cochrane ...
journal_title:International journal of colorectal disease
pub_type: 杂志文章,评审
doi:10.1007/s00384-013-1784-8
更新日期:2014-03-01 00:00:00
abstract::Although the cause of Crohn's disease remains obscure, considerable progress has been made in recent years in unraveling the pathogenesis of the inflammatory processes seen in chronic idiopathic inflammatory bowel diseases. Th-1 lymphocytes seem to orchestrate the inflammation through the production of proinflammatory...
journal_title:International journal of colorectal disease
pub_type: 杂志文章,评审
doi:10.1007/s003840050182
更新日期:1999-02-01 00:00:00