Abstract:
:A number of tumor suppressor and tumor-related genes exhibit promoter hypermethylation with resulting gene silencing in human cancers. In addition, several gene promoters have also been shown to become hypermethylated in non-neoplastic cells during aging. To assess the physiological consequence and clinical significance of gene promoter methylation in gastric epithelia, our laboratory has studied the methylation status of tumor suppressor and tumor-related genes, including APC, DAP-kinase, DCC, E-cadherin, GSTP1, hMLH1, p16, PTEN, RASSF1A, RUNX3 and TSLC1, in neoplastic and non-neoplastic gastric epithelia. The tumor suppressor and tumor-related genes, except APC, were generally unmethylated in non-neoplastic gastric epithelia obtained from younger individuals. The frequencies of methylation increased with age to varying degrees in various genes, although GSTP1 and PTEN methylation was completely absent in both neoplastic and non-neoplastic gastric epithelia. The methylation frequencies in each gene were found to be comparable in neoplastic and non-neoplastic gastric epithelia, except the methylation of RUNX3 and TSLC1, which was mostly cancer-specific (P<0.01). When methylation frequencies were compared between non-neoplastic gastric epithelia from cancer-bearing and non-cancer-bearing stomachs, hMLH1 and p16 methylation was more frequent in those from cancer-bearing stomachs (P<0.01). Promoter methylation in tumor suppressor and tumor-related genes initially occurs in non-neoplastic gastric epithelia, increases with age, and ultimately silences gene function to constitute a field-defect that may predispose tissues to gastric cancer evolution. In clinical applications RUNX3 and TSLC1 methylation may be utilized as molecular diagnostic markers, and hMLH1 and p16 methylation as predictors of malignancy in the stomach.
journal_name
Histol Histopatholjournal_title
Histology and histopathologyauthors
Tamura Gdoi
10.14670/HH-19.221subject
Has Abstractpub_date
2004-01-01 00:00:00pages
221-8issue
1eissn
0213-3911issn
1699-5848journal_volume
19pub_type
杂志文章,评审abstract::A novel family of evolutionally conserved neuronal guidance cues, including ligands (i.e., Slit, netrin, epherin, and semaphorin) and their corresponding receptors (i.e., Robo, DCC/Unc5, Eph and plexin/ neuropilin), has been identified to play a crucial role in axon pathfinding and branching as well as neuronal cell m...
journal_title:Histology and histopathology
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
pub_type: 杂志文章
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
pub_type: 杂志文章,评审
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journal_title:Histology and histopathology
pub_type: 杂志文章,评审
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journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-11-778
更新日期:2017-02-01 00:00:00
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journal_title:Histology and histopathology
pub_type: 杂志文章,评审
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journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-19.391
更新日期:2004-04-01 00:00:00
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journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-11-762
更新日期:2016-08-01 00:00:00
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journal_title:Histology and histopathology
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更新日期:1991-10-01 00:00:00
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
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journal_title:Histology and histopathology
pub_type: 杂志文章,评审
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
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doi:10.14670/HH-26.1187
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
pub_type: 杂志文章,评审
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更新日期:2012-02-01 00:00:00
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
pub_type: 杂志文章,评审
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更新日期:2018-07-01 00:00:00