Abstract:
:A new coding sequence of the procarboxypeptidase B gene was obtained from SD rat fresh pancreas by RT-PCR and highly expressed in Escherichia coli in inclusion bodies. The folded procarboxypeptidase B was subjected to trypsin enzymatic cleavage to produce active carboxypeptidase B, subsequently, carboxypeptidase B was effectively purified with anion exchange chromatography DEAE-FF and hydrophobic interaction chromatography Octyl FF, as a result, 40 mg carboxypeptidase B per litre cell culture with specific activity 7.42 u/mg was achieved. Further research showed that the obtained recombinant carboxypeptidase B could substitute carboxypeptidase B isolated from pancreas.
journal_name
Protein Pept Lettjournal_title
Protein and peptide lettersauthors
Su-Xia L,Yu-Jian Z,Li-Ping T,Qin-Sheng Y,Yi Gdoi
10.2174/0929866033478627subject
Has Abstractpub_date
2003-12-01 00:00:00pages
581-90issue
6eissn
0929-8665issn
1875-5305journal_volume
10pub_type
杂志文章abstract:BACKGROUND:Development of proteins and peptides into drugs has been considered as a promising strategy to target certain diseases. However, only few proteins and peptides have been approved as new drugs into the market each year. One major problem is that proteins and peptides often exhibit short plasma half-life times...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866525666180530122835
更新日期:2018-01-01 00:00:00
abstract::Proteases have been used not only for proteolysis but also in organic solvent-assisted religation processes. Here, we demonstrated the effect of salts on peptide bond resynthesis in Glutathione-S-transferase (GST) and have found it to be in the purview of the Hofmeister phenomena. Our results show that the efficiency ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986610789909395
更新日期:2010-01-01 00:00:00
abstract::For biophysical studies using heteronuclear NMR analysis of amyloid beta peptide, construction of an efficient and high yield expression system of uniformly isotopic labeled amyloid beta peptide is desirable. Here we succeeded in obtaining (15)N-labeled amyloid beta 1-40 expressed by attachment to hen egg white lysozy...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986607781483741
更新日期:2007-01-01 00:00:00
abstract::The superoxide anion radical is a highly reactive toxic species produced during the metabolic processes. A number of copper (II) complexes with amino acids and peptides are known to show superoxide dismutase (SOD) like activity. The design and application of synthetic low molecular weight metal complexes as SOD mimics...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986611797642724
更新日期:2011-12-01 00:00:00
abstract::Human butyrylcholinesterase (BuChE) is a serine enzyme present in most organs and plasma. No clear physiological function has yet been assigned to BuChE, but it is a pharmacologically and toxicologically important enzyme that plays a role in degradation of numerous ester-containing drugs and poisonous esters. Thus, Bu...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/092986609789071207
更新日期:2009-01-01 00:00:00
abstract::Infections by antibiotic-resistant bacteria are becoming a great risk for human health, leading to an urgent need for new efficient antibacterial therapies. The short, proline-rich antimicrobial peptides from insects gained a lot of interest as a potential antibacterial treatment, having a low toxicity profile and bei...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/09298665113206660110
更新日期:2014-04-01 00:00:00
abstract::A computer-aided docking study was carried out to quickly clarify the binding structure of the ligand-receptor complex between bisphenol A (BPA), a well-known endocrine disruptor, and estrogen-related receptor gamma (ERRgamma). The resulting complex indicated that BPA binds to the ligand-binding pocket of ERRgamma wit...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608783744261
更新日期:2008-01-01 00:00:00
abstract::The stability of Merrifield linker in Fmoc deprotection process was quantitatively investigated by establishing working curve of two major decomposition components from two resin bound dipeptide models. By sampling reaction solution and analyzing with RP-HPLC, decomposition rate was determined. The results indicated t...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986613804725343
更新日期:2013-02-01 00:00:00
abstract::The physiological stability curve--a plot of the free energy of unfolding versus temperature--is calculated for hen egg white lysozyme from a combination of extrapolated unfolding thermodynamic data from reversible conditions and isothermal titrations with guanidine hydrochloride. The shape of the curve suggests the e...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986606777841163
更新日期:2006-01-01 00:00:00
abstract::The etiology of multiple sclerosis (MS) remains unclear. To determine if autoantibodies to myelin basic protein (MBP) are produced during parvovirus B19 infection, a competitive ELISA was performed using plasma from MS patients exhibiting high IgG titers for parvovirus. Our results showed the addition of MBP decreased...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986606775101715
更新日期:2006-01-01 00:00:00
abstract::VML is a lectin from Vatairea macrocarpa seeds that has various biological activities. Here, we describe three new lectin isoforms from V. macrocarpa identified through genomic DNA analysis. One of these isoforms has high similarity to VML, while another that has noteworthy differences. We have denoted the new isoform...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/09298665113209990049
更新日期:2013-11-01 00:00:00
abstract::Classification of newly determined protein structures is important in understanding their function and mechanism of action. Currently available methods employ a global structure alignment strategy and are computationally expensive. We propose a two-step methodology with a quick screen to significantly reduce the numbe...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986607781483877
更新日期:2007-01-01 00:00:00
abstract::Retro-ACP (74-65) fragment was synthesized on a novel 4% tri-(propylene glycol) glycerolate diacrylate cross-linked polystyrene (PS-TRPGGDA) support. The peptide is grown from the functional site present in the cross-linker, which makes it unique and cost-effective among other styrene based polymer supports. A compara...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866033478753
更新日期:2003-10-01 00:00:00
abstract:BACKGROUND:Chickpea is a widely grown legume in India, Australia, Canada, and Mediterranean regions. Seeds of chickpea are good source of protein for both human and animals. Wild relatives of chickpea (Cicer arietinum) are the potential gene pool for crop improvement; however, very little information is available on th...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866527666200910164118
更新日期:2020-09-10 00:00:00
abstract::The mutation, conferring streptomycin and deoxyglucose resistance on cells, had profound effect on the kinetic and thermodynamic parameters inferring thermostabilization of beta-glucosidase from mutant 51 SM(r) of Cellulomonas biazotea. Free energy of activation for substrate binding, enthalpy and entropy of activatio...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866053587183
更新日期:2005-04-01 00:00:00
abstract::Coiled coils are important structural modules and domains of protein-protein interactions. Further, they provide the required framework to proteins, like kinesins, myosins and SNAREs, which are either structural or involved in transport of biomolecules. We provide an interactive webserver to measure the strength of in...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608783330314
更新日期:2008-01-01 00:00:00
abstract::Antimicrobial peptides (AMPs) constitute an important alternative in the search for new treatments against pathogens. We analyzed the sequence variability in cytokine and chemokine proteins to investigate whether these molecules contain a sequence useful in the development of new AMPs. Cluster analysis allowed the ide...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866511320050001
更新日期:2013-05-01 00:00:00
abstract::Identification on protein folding types is always based on the 27-class folds dataset, which was provided by Ding & Dubchak in 2001. But with the avalanche of protein sequences, fold data is also expanding, so it will be the inevitable trend to improve the existing dataset and expand more folding types. In this paper,...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986612799789378
更新日期:2012-04-01 00:00:00
abstract::DrTI was effective against trypsin-like enzymes from A. kuehniella and C. cephalonica, however an artificial diet was insufficient to affect the survival and body weight of either insect. The inhibitor stimulated chymotrypsin-like enzymes and probably induced the synthesis of enzymes insensitive to TLCK in neonate lar...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609789839395
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:A core sequence (the 9 C-terminal residues) of calcification-associated peptide (CAP- 1) isolated from the exoskeleton of the red swamp crayfish was previously shown to control calcium carbonate precipitation with chitin. In addition, a modified core sequence in which the phosphorylated serine at the N termi...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866525666171221114658
更新日期:2018-01-01 00:00:00
abstract::PepX is a X-prolyl dipeptidyl aminopeptidase of S15 family that cleaves dipeptides from the N-terminus of polypeptides having a proline or alanine residue at the second position. Involved in bacterial nutrition and in peptide maturation, this serine exopeptidase, counterpart of the mammalian DDP-4, has been proposed t...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract::Here, we show for the first time that non-fibrillar and spherical aggregates produced from PrP-(23-98) in the presence of NADPH plus copper ions are toxic to cultured cells and induce apoptotic signals. It is also confirmed that endogenous cellular PrP isoform is not required for toxicity to occur. ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609787049475
更新日期:2009-01-01 00:00:00
abstract::Quantum mechanical fragment molecular orbital calculations have been performed for receptor binding of the hemagglutinin protein of the recently pandemic influenza 2009 H1N1, A/swine/Iowa/1930, and A/Puerto Rico/8/1934 viruses to α2-6 linked sialyloligosaccharides, as analogs of human receptors. The strongest receptor...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986611794927893
更新日期:2011-05-01 00:00:00
abstract::Though protein folding is a regular phenomenon inside the cellular milieu, slight differences in the folding pattern of proteins may lead to disease-causing forms. Many diseases have been identified that are caused by these misfolded macromolecules and a considerable amount of focus is still directed towards better un...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866043406409
更新日期:2004-12-01 00:00:00
abstract::Chemical probing of histidine residues using specific modifiers, iodoacetic acid (IAA) and diethylpyrocarbonate (DEP) resulted in the inactivation of phytase (phy A). The kinetic theory of the substrate reaction during the modification of enzyme activity was applied to a study of the kinetics of the course of inactiva...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986606777145788
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Cryptochrome is a flavin-binding blue-light photoreceptor that functions in growth and development in plants, the circadian clock in animals and navigation in birds. However, a lack of purified cryptochrome has hindered studies of the structure and function of this protein. In this study, we obtained a subst...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866525666181004112308
更新日期:2018-01-01 00:00:00
abstract::Protein palmitoylation is an important and common post-translational lipid modification of proteins and plays a critical role in various cellular processes. Identification of Palmitoylation sites is fundamental to decipher the mechanisms of these biological processes. However, experimental determination of palmitoylat...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986611794475084
更新日期:2011-02-01 00:00:00
abstract::Application of complementary B and T cell epitopes in inducing anti-idiotypic and anti-clonotypic antibodies capable of regulating or suppressing the autoimmune responses in experimental autoimmune myasthenia gravis (EAMG), allergic neuritis (EAN) and allergic encephalomyelitis (EAE) has been the stimulus of many rese...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866043407020
更新日期:2004-08-01 00:00:00
abstract::Catalase is an enzyme that occurs in almost all aerobic organisms. Its main metabolic function is to prevent oxidative damage to tissues induced by hydrogen peroxide which is a strong oxidizing agent. Catalase is very effective in performing this task, since it has the highest turnover rate among all the enzymes. The ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986611796011392
更新日期:2011-09-01 00:00:00
abstract:BACKGROUND:Multiple Sequence Alignment (MSA) is a fundamental task in bioinformatics and is required for many biological analysis tasks. The more accurate the alignments are, the more credible the downstream analyses. Most protein MSA algorithms realign an alignment to refine it by dividing it into two groups horizonta...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866526666190806143959
更新日期:2020-01-01 00:00:00