Polymerase chain reaction detection of Neisseria meningitidis in the intraocular fluid of a patient with endogenous endophthalmitis but without associated meningitis.

Abstract:

PURPOSE:To report a patient with Neisseria meningitidis endophthalmitis without associated meningitis with full visual recovery, with early detection of the microorganism using polymerase chain reaction (PCR) analysis. DESIGN:Retrospective, observational case report. PARTICIPANTS:One patient with endogenous endophthalmitis. METHODS:Polymerase chain reaction analysis and culture of the intraocular fluid sample. Polymerase chain reaction analysis was performed with a universal (16S rRNA) primer set to detect bacterial DNA, and subsequently a specific probe was used to detect Neisseria species DNA. RESULTS:The 16S rRNA primers detected bacterial DNA, the specific probe detected Neisseria species DNA, and culture was positive for Neisseria meningitidis serotype C. CONCLUSIONS:A universal bacterial PCR can be very helpful for the diagnosis of endogenous bacterial endophthalmitis at an early stage of the disease.

journal_name

Ophthalmology

journal_title

Ophthalmology

authors

Kerkhoff FT,van der Zee A,Bergmans AM,Rothova A

doi

10.1016/S0161-6420(03)00834-0

subject

Has Abstract

pub_date

2003-11-01 00:00:00

pages

2134-6

issue

11

eissn

0161-6420

issn

1549-4713

pii

S0161-6420(03)00834-0

journal_volume

110

pub_type

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