Abstract:
:Polyadenosylation of nuclear enzymes is well known to regulate the cellular repair capacity after DNA damage. PARP mediates the transfer of poly-ADP-ribose moieties on itself and other nuclear proteins by the breakdown of NAD+. The present study investigated how modulation of PARP activity interferes with cell death induced by two different alkylating agents used in cancer chemotherapy. 1-methyl-3-nitro-1-nitrosoguanidinium (MNNG) decreased cellular reduction capacity (WST-1 assay) in HL60 and CCRF-CEM cells, accompanied by increased activity of PARP and depletion of intracellular NAD+ and ATP. Pretreatment with the PARP inhibitors 3-AB or 4-AN resulted in transient cell protection, which was associated with a switch from necrosis to apoptosis in CCRF-CEM cells and enhanced apoptosis in HL60 cells. Both PARP inhibitors delayed the drop in WST-1 reduction and retained NAD+ and ATP levels required for apoptosis. Furthermore, 3-AB or 4-AN prevented progressive DNA degradation in MNNG-treated CCRF-CEM cells. In contrast to MNNG, we did not observe early activation of PARP, decrease in WST-1 reduction, or wasteful consumption of NAD+ and ATP after treatment with melphalan. However, preincubation with 3-AB or 4-AN resulted in decreased HL60 cell membrane blebbing and reduced formation of apoptotic bodies. In conclusion, the cell death preventing effects of PARP inhibitors are mediated by their ability to maintain cellular energy metabolism, to inhibit the activation of endonucleolytic DNA degradation and to prevent cell blebbing. Surprisingly, these protective effects of PARP inhibitors on different cell functions seem to be independent of each other and are rather determined by the respective cytotoxic mechanisms implicated by different drugs. Our results support the hypothesis, that PARP activation and/or cleavage plays a regulatory role in the induction of apoptosis.
journal_name
Eur J Med Resjournal_title
European journal of medical researchauthors
Pogrebniak A,Schemainda I,Pelka-Fleischer R,Nüssler V,Hasmann Msubject
Has Abstractpub_date
2003-10-22 00:00:00pages
438-50issue
10eissn
0949-2321issn
2047-783Xjournal_volume
8pub_type
杂志文章abstract::Among the 763 patients with ulcers and stomach cancer, including the control group of 586 blood donors, the search for a relationship between the spread of infection and the main blood groups was defined by the appearance of IgG against H. pylori in blood plasma (the ELISA test). The statistical analysis, however, did...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:1996-03-19 00:00:00
abstract::A number of neurodegenerative diseases including Parkinson's disease (PD) have been shown to be associated with polymorphic variants of mitochondrial DNA. The A4336G mutation in the tRNA(Gln) gene was reported to occur at a higher frequency in individuals with Parkinson's disease and Alzheimer's disease (AD) than in a...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:1997-03-24 00:00:00
abstract:BACKGROUND:In subtotal central retinal artery occlusion (CRAO), distinct retinal edema with acute onset irreversible visual loss is common. - METHODS:Clinical observation, fluorescein angiogram. - PATIENT:A 69-year-old patient presented with acute visual loss of his right eye. Risk factors for CRAO were arterial hype...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2006-01-31 00:00:00
abstract:OBJECTIVE:To compare two reduced dose indinavir (IDV) + ritonavir (RTV) combinations guided by therapeutic drug monitoring (TDM) in treatment-naive HIV1-infected patients. METHODS:HIV1-infected treatment naive patients were prospectively randomized to treatment with IDV 600 mg or 400 mg BID each in combination with RT...
journal_title:European journal of medical research
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2007-07-26 00:00:00
abstract:BACKGROUND:The purpose was to explore possible risk factors of facet joint violation induced by adjacent superior vertebral pedicle screw during the minimally invasive surgery transforaminal lumbar interbody fusion (MIS-TLIF). METHODS:A total of 69 patients with lumbar degenerative disease, who underwent MIS-TLIF were...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-015-0174-9
更新日期:2015-09-24 00:00:00
abstract::In the treatment of pulmonary diseases the inhalation of aerosols plays a key role - it is the preferred route of drug delivery in asthma, chronic obstructive pulmonary disease (COPD) and cystic fibrosis. But, in contrast to oral and intravenous administration drug delivery to the lungs is controlled by additional par...
journal_title:European journal of medical research
pub_type: 杂志文章,评审
doi:10.1186/2047-783x-14-s4-71
更新日期:2009-12-07 00:00:00
abstract::So far, neither treatment with standard unfractionated heparin (UFH) nor with low-molecular-weight heparin (LMWH) has been shown to reduce mortality or to improve neurological outcome in patients with acute ischemic stroke. Although a reduction of early recurrent stroke has been demonstrated for the use of subcutaneou...
journal_title:European journal of medical research
pub_type: 杂志文章,评审
doi:
更新日期:2004-04-30 00:00:00
abstract::Plexiform neurofibroma (PN) is a rare benign tumor and a special subtype of neurofibromatosis type 1 (NF1). Though the incidence is low, giant PN of the craniomaxillofacial region could result in severe hemifacial hypertrophy which is known as a typical manifestation of NF1 in young children. Here, we retrospectively ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783x-15-2-84
更新日期:2010-02-26 00:00:00
abstract:OBJECTIVE:Changes in therapeutic concepts can only be justified by a significant improvement of outcome parameters. Furthermore, detailed statistics of complications are needed to guarantee high quality of treatment. This study describes the new University of Munich Lung Cancer Group Database. METHODS:The MLCG-Databas...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2007-10-30 00:00:00
abstract:BACKGROUND:Postoperative hyperthyroidism occurs in approximately one third of patients following parathyroidectomy due to primary hyperparathyroidism (PHP), but has only rarely been described in secondary hyperparathyroidism (SHP). The frequency, course, and laboratory markers of postoperative hyperthyroidism in SHP re...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783x-16-8-375
更新日期:2011-08-08 00:00:00
abstract:BACKGROUND:Several studies have been performed in tumour patients to analyse the immunological response to mistletoe extracts. Considering the fact that these extracts are given subcutaneously in most instances, the kind of application resembles a typical immunization schedule. We therefore wanted to see how those extr...
journal_title:European journal of medical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2002-04-30 00:00:00
abstract::The purpose of the present study was to evaluate clinically the effect of an anaesthetic gel (lidocaine 20 mg/g as active agent) on pain sensitivity and early wound healing following nonsurgical periodontal therapy. A total of 40 patients with chronic periodontitis were enrolled in this randomized, split-mouth, double...
journal_title:European journal of medical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2007-05-29 00:00:00
abstract::Under antidepressive treatment with amitryptiline (100 mg/d) a 71-year old woman developed delirious symptoms, hyponatremia and a grand mal seizure followed by cardiovascular arrest. A few month later she ingested 48 mg reboxetine with suicidal intent. Overdosing of reboxetine, a selective noradrenaline re-uptake inhi...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2002-09-30 00:00:00
abstract:BACKGROUND:Prophylaxis efforts for the maintenance of tooth health led to a distinctive decrease in caries frequency with children and young persons. Because the toothbrush can still be described as the most important oral hygiene aid, possible differences between the effectivity of electric and manual toothbrushes sho...
journal_title:European journal of medical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2001-01-29 00:00:00
abstract::The 825T-allele of the C825T polymorphism in GNB3, the gene for the G protein beta3 subunit, has been reported to be associated with essential hypertension and obesity. Expression of Gbeta3s, the gene product of GNB3 associated with the GNB3 825T-allele, causes increased signal transduction which may contribute to pat...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2003-03-27 00:00:00
abstract:BACKGROUND:Prosthetic joint infection (PJI) is a serious complication of orthopedic implant surgery. Treatment often includes the use of an antibiotic-loaded Polymethyl methacrylate (PMMA) bone cement spacer. Several antibiotics are commonly used for the preparation of these spacers, but due to the increasing number of...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-020-00428-z
更新日期:2020-08-18 00:00:00
abstract::Human Immunodeficiency virus can affect the peripheral (PNS) and central nervous system (CNS). In the ART / HAART era especially the CNS is said to be a virus reservoir so that neurologists have to be more and more integrated in therapy planning. Therefore the present paper describes neurological indications for ART, ...
journal_title:European journal of medical research
pub_type: 杂志文章,评审
doi:
更新日期:1999-11-22 00:00:00
abstract::Exposure to high concentrations of oxygen is known to induce changes in lung function through effects on several pulmonary cell types, including alveolar macrophages (AM). In this study, we studied the in vitro effects of hyperoxia on the release of proinflammatory cytokines and the expression of surface receptors in ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:1997-04-21 00:00:00
abstract:BACKGROUND:Recent studies were able to demonstrate involvement of the complement cascade in bone biology. Further studies analyzed the role of complement in traumatic injuries and demonstrated negative effects after excessive systemic activation of the inflammatory response with early abrogation of complement activatio...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-016-0236-7
更新日期:2016-10-26 00:00:00
abstract:OBJECTIVE:given sparse research on the issue, this study sought to shed light upon the interactions of alexithymia, emotion processing, and social anxiety in adults with attention-deficit hyperactivity disorder (ADHD). SUBJECTS AND METHODS:73 German adults with ADHD according to DSM-IV diagnostic criteria participated...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783x-15-9-403
更新日期:2010-09-24 00:00:00
abstract::Triple A (four A; Allgrove syndrome) syndrome is a an autosomal recessive disorder characterized by alacrimia, achalasia, adrenocortical insufficiency, and various neurological abnormalities. We report a case of triple A syndrome in a 14-year-old female patient together with oral manifestations. The importance of this...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2003-07-31 00:00:00
abstract:BACKGROUND:Dopamine agonists (DA) are the first-choice drug for treatment of the early stage of Parkinson's disease (PD) in subjects younger than 70 years. Recently, a number of third generation DA have been marketed, including transdermal patch of rotigotine and extended release oral formulation of ropinirole and pram...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783X-18-60
更新日期:2013-12-21 00:00:00
abstract:BACKGROUND:The purpose of this study was to assess the association between leisure-time physical activity (LPA) and the risk of metabolic syndrome (MS). METHODS:Prospective cohort studies of the association between LPA and the risk of MS were retrieved from the PubMed and Embase databases up to 12 August 2013. The sta...
journal_title:European journal of medical research
pub_type: 杂志文章,meta分析,收录出版
doi:10.1186/2047-783X-19-22
更新日期:2014-04-23 00:00:00
abstract:BACKGROUND:Increasing rates of prosthetic joint infection (PJI) have presented challenges for general practitioners, orthopedic surgeons and the health care system in the recent years. The diagnosis of PJI is complex; multiple diagnostic tools are used in the attempt to correctly diagnose PJI. Evidence-based algorithms...
journal_title:European journal of medical research
pub_type: 杂志文章,meta分析
doi:10.1186/s40001-017-0245-1
更新日期:2017-03-09 00:00:00
abstract::Acute liver failure represents one of the most challenging conditions in intensive care treatment. In most cases there is no causal medical therapy available for survive making the intensive care treatment as the most important management tool, as bridge to transplant or still the recovery of the liver! These patients...
journal_title:European journal of medical research
pub_type: 杂志文章,评审
doi:
更新日期:2004-05-28 00:00:00
abstract:OBJECTIVE:The influence of cytomechanical forces in cellular migration, proliferation and differentiation of mesenchymal stem cells (MSCs) is still poorly understood in detail. METHODS:Human MSCs were isolated and cultivated onto the surface of a 3 x 3 mm porcine collagen I / III carrier. After incubation, cell cultur...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783x-15-1-35
更新日期:2010-01-29 00:00:00
abstract:BACKGROUND:We aimed to analyze the association between hypertension and deep vein thrombosis (DVT) after orthopedic surgery. METHODS:Relevant studies were identified by a search of PubMed, Embase, China National Knowledge Infrastructure, Wanfang, the Chinese Biomedical Literature, and Weipu database until December 201...
journal_title:European journal of medical research
pub_type: 杂志文章,meta分析
doi:10.1186/s40001-016-0207-z
更新日期:2016-03-22 00:00:00
abstract::CCR5 receptor inhibitors are currently being introduced into clinical practice. In some instances treatment failure is related to the selection of pre-existing CXCR4-tropic minority virus strains. Up to date it is unclear whether the outgrowth of a CXCR4 using reservoir is associated with accelerated HIV-disease. In a...
journal_title:European journal of medical research
pub_type: 杂志文章,评审
doi:
更新日期:2007-10-15 00:00:00
abstract::Endorectal ultrasonography (ERUS) and magnetic resonance imaging (MRI) allow exploring the morphology of the rectum in detail. Use of such data, especially assessment of the rectal wall, is an important tool for ascertaining the perianal fistula localization as well as stage of the cancer and planning it appropriate t...
journal_title:European journal of medical research
pub_type: 杂志文章,评审
doi:10.1186/s40001-014-0078-0
更新日期:2015-01-14 00:00:00
abstract:BACKGROUND:A great variety of dental materials are now available for use in restorative treatments. Much attention has been devoted to physical and chemical properties, but the biological effects of these materials have been less frequently studied. AIM:The aim of this study is to test the influence of resin based res...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2001-10-29 00:00:00