Abstract:
:HIV-1 positive patients generate Nef-specific CTL response, indicating that Nef is a potent immunogen. However, Nef is also known to down regulate the expression of CD4 and MHC-I molecules, thereby protecting virally infected target cells. We compared the immunogenicity of non-functional nef vaccine constructs to wild type functional nef as potential immunogen. Mice were immunized with different nef constructs and assessed for their ability to induce cellular immune responses. Evaluation of T cell immune responses in mice showed that non-functional nef vaccine constructs are capable of inducing a significant T cell immune response measured by IFN-gamma ELISPOT. Further epitope mapping studies indicate that one of our attenuated constructs, Nef R-38, has multiple CTL epitopes spanning throughout the gene. Our results indicate that functionally attenuated Nef antigen might be a better candidate for future multiprotein HIV-1 vaccine.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Majumder B,Gray B,McBurney S,Schaefer TM,Dentchev T,Mahalingam S,Reinhart TA,Ayyavoo Vdoi
10.1016/s0165-2478(03)00141-xsubject
Has Abstractpub_date
2003-10-31 00:00:00pages
207-14issue
2-3eissn
0165-2478issn
1879-0542pii
S016524780300141Xjournal_volume
89pub_type
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pub_type: 临床试验,杂志文章
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更新日期:2018-02-01 00:00:00
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journal_title:Immunology letters
pub_type: 杂志文章
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journal_title:Immunology letters
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pub_type: 杂志文章
doi:10.1016/j.imlet.2008.03.001
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journal_title:Immunology letters
pub_type: 杂志文章
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journal_title:Immunology letters
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journal_title:Immunology letters
pub_type: 杂志文章
doi:
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journal_title:Immunology letters
pub_type: 杂志文章
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pub_type: 杂志文章
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