Prostaglandin E2 inhibits TNF production in murine bone marrow-derived dendritic cells.

Abstract:

:Exposure to pathogens induces dendritic cells to release inflammatory cytokines and chemokines. The inflammatory response is controlled by endogenous agents such as anti-inflammatory cytokines, glucocorticoids, anti-inflammatory neuropeptides, and lipid mediators. This study is the first report on the inhibition by prostaglandin E2 (PGE2) of TNF release from bone marrow-derived dendritic cells stimulated with lipopolysaccharide (LPS), a TLR4 ligand, or peptidoglycan, a TLR2 ligand. The inhibition of TNF occurs at both mRNA and protein level. The inhibitory effect of PGE2 is mediated by the EP2 and EP4 receptors, and involves both PKA signaling and mediation by DC-derived IL-10. Intraperitoneal administration of PGE2 together with LPS results in a reduction in serum TNF and intracellular TNF in peritoneal exudate cells, compared to LPS alone. In addition, administration of PGE2 in vivo reduces the numbers of CD11c+ DCc that accumulate in the peritoneal cavity in response to LPS. The various implications of the PGE2-induced reduction in TNF are discussed.

journal_name

Cell Immunol

journal_title

Cellular immunology

authors

Vassiliou E,Jing H,Ganea D

doi

10.1016/s0008-8749(03)00158-8

subject

Has Abstract

pub_date

2003-06-01 00:00:00

pages

120-32

issue

2

eissn

0008-8749

issn

1090-2163

pii

S0008874903001588

journal_volume

223

pub_type

杂志文章
  • Proliferative effects of 12-O-tetradecanoylphorbol-13-acetate (TPA) and calcium ionophores on human large granular lymphocytes (LGL).

    abstract::The proliferative responses of natural killer (NK) cells to 12-O-tetradecanoylphorbol-13-acetate (TPA), which directly activates protein kinase c(PKC), and to the Ca2+ ionophores A23817 and ionomycin, known to enhance the intracellular calcium, have been investigated. Highly purified large granular lymphocytes (LGL) w...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(88)90007-x

    authors: Procopio A,Gismondi A,Paolini R,Morrone S,Testi R,Piccoli M,Frati L,Herberman RB,Santoni A

    更新日期:1988-04-15 00:00:00

  • Changes in purine nucleoside phosphorylase activity during thymosin-induced human null cell differentiation.

    abstract::Purine nucleoside phosphorylase (PNP) is a purine salvage pathway enzyme which we have found to be 8-10 times more active (per cell) in human peripheral blood null lymphocytes than in T lymphocytes. To test the hypothesis that null cells are, in part, pre-T lymphocytes we have defined an in vitro system for null cell ...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(83)90288-5

    authors: Cowan MJ,Fraga M,Ammann AJ

    更新日期:1983-06-01 00:00:00

  • Chemotactic subpopulation of macrophage cell line cells (M1 cells) discerned by three macrophage chemotactic factors from delayed hypersensitivity reaction sites.

    abstract::The functional specificity of three types of macrophage chemotactic factors ( MCFs ), -a, -b, and -c, from purified protein derivative of tuberculin (PPD)-induced delayed hypersensitivity reaction (DHR) skin sites on guinea pigs, was analyzed using macrophage cell line cells, M1, established from myeloid leukemia cell...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(84)90354-x

    authors: Honda M,Masuda T,Yoshimura T,Hayashi H

    更新日期:1984-06-01 00:00:00

  • Differential effect of cyclosporin-A on the mixed-lymphocyte culture-induced proliferative and cytotoxic responses of T lymphocytes with different cell densities.

    abstract::We have analyzed the effect of cyclosporin-A (CsA) on the proliferative and cytotoxic responses induced by mixed-lymphocyte cultures (MLC-s) on low and high density T lymphocytes. Allogeneic stimulation had a different impact on the two subsets. Proliferative and cytotoxic responses were inversely correlated; i.e., hi...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(86)90100-0

    authors: Bejarano MT,Masucci MG,Klein E

    更新日期:1986-12-01 00:00:00

  • Essential role of TNF-alpha in development of spleen fibroblastic reticular cells.

    abstract::TNF-alpha plays an important role in the development of secondary lymphoid tissues. Earlier studies showed that fibroblastic reticular cells express TNF-alpha receptor, suggesting that TNF-alpha may affect the development of FRCs. To test this, we analyzed the development and function of FRCs in wild-type or TNF-alpha...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/j.cellimm.2015.01.006

    authors: Zhao L,Chen J,Liu L,Gao J,Guo B,Zhu B

    更新日期:2015-02-01 00:00:00

  • Enhancement of antigen-induced interleukin 4 and IgE production by specific IgG1 in murine lymphocytes.

    abstract::Conalbumin (CA)-specific type 2 helper T cell (Th2) clone, D10G4.1 (D10) produces IL4 when stimulated with varying doses of TNP-CA in the presence of mitomycin C-treated C3H spleen cells or purified B cells as antigen-presenting cells (APC). The production of IL4 was assessed by bioassay and by expression of IL4 mRNA....

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(92)90333-k

    authors: Ohmori H,Hase N,Hikida M,Takai T,Endo N

    更新日期:1992-12-01 00:00:00

  • Tissue distribution and appearance in ontogeny of alpha/beta T cell receptor (TCR2) in chicken.

    abstract::We have performed immunoperoxidase staining on cryostat tissue sections and immunofluorescence analysis on cell suspensions to identify cells expressing the alpha/beta T cell antigen receptor during ontogeny and adult life in chickens. We used the mouse monoclonal antibody, TCR2, which was previously shown to recogniz...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(90)90079-7

    authors: Vainio O,Lassila O,Cihak J,Lösch U,Houssaint E

    更新日期:1990-01-01 00:00:00

  • Comparison of linear and branched peptide forms (MAPs) in the induction of T helper responses to point-mutated ras immunogens.

    abstract::The utility of multiple antigenic peptides (MAPs) for the induction of antibody and cellular immune responses in animal models has been demonstrated for a variety of peptide epitopes involved in human disease. However, little is known about immune responses to MAPs constructed with antigenic tumor epitopes, nor has pe...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1006/cimm.1996.0310

    authors: Schott ME,Wells DT,Schlom J,Abrams SI

    更新日期:1996-12-15 00:00:00

  • Phenotypic and functional changes of GM-CSF differentiated human macrophages following exposure to apoptotic neutrophils.

    abstract::The engulfment of apoptotic cells by monocytes and unprimed macrophages results in M2 polarization. In the current study, we investigated whether apoptotic cells influence the phenotypic and functional characteristics of GM-CSF-differentiated human macrophages (GM-Mφ). Our results demonstrate that GM-Mφ preincubated w...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/j.cellimm.2018.06.002

    authors: Chernykh ER,Sakhno LV,Shevela EY,Tikhonova MA,Khonina NA,Ostanin AA

    更新日期:2018-09-01 00:00:00

  • Properties of anti-Lyb-2-mediated B-cell activation and the relationship between Lyb-2 molecules and receptors for B-cell stimulatory factor-1 on murine B lymphocytes.

    abstract::The murine B-cell differentiation antigen Lyb-2 has been shown to be involved in B-lymphocyte activation and has been postulated by some to be related to a receptor for B-cell stimulatory factor I (BSF-1) (H. Yakura et al., J. Immunol. 137, 1475, 1986). Here we have demonstrated that monoclonal antibody (mAB) to Lyb-2...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(88)90302-4

    authors: Subbarao B,Morris J,Baluyut AR

    更新日期:1988-04-01 00:00:00

  • Class I-like CD1A-C do not protect target cells from NK-mediated cytolysis.

    abstract::Previous studies have validated the capacity of classical (HLA-A,B,C) and nonclassical (HLA-G) MHC class I antigens expressed by target cells to influence natural killer (NK) cell-mediated cytolysis. Generally, elevated expression of these HLA class I molecules is correlated with enhanced resistance to lysis by NK cel...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1006/cimm.1996.0020

    authors: Storkus WJ,Wei M,Cresswell P,Dawson JR

    更新日期:1996-01-10 00:00:00

  • Expression of delta opioid receptors by splenocytes from SEB-treated mice and effects on phosphorylation of MAP kinase.

    abstract::Delta opioid receptors (DORs) are known to modulate multiple T-cell responses. However, little is known about the expression of these receptors. These studies evaluated the expression of DOR mRNA and protein after a single in vivo exposure to staphylococcal enterotoxin B (SEB). SEB (20 microg, ip) significantly enhanc...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1006/cimm.2000.1717

    authors: Shahabi NA,McAllen K,Matta SG,Sharp BM

    更新日期:2000-11-01 00:00:00

  • Role of macrophages as modulators but not as autonomous accessory cells in primary antibody response.

    abstract::The role of macrophages (M phi) in the in vitro primary antibody response of murine lymphocytes to sheep erythrocytes was investigated. Peritoneal M phi were activated to express Ia antigens either in vitro or in vivo. Nonactivated Ia- M phi were also examined. We observed that only Ia- M phi but also Ia+ M phi failed...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(85)90316-8

    authors: Komatsubara S,Hirayama Y,Inaba K,Naito K,Yoshida K,Kawai J,Muramatsu S

    更新日期:1985-10-15 00:00:00

  • Bcl-2 expression in the thymus and periphery.

    abstract::Bcl-2 proto-oncogene is expressed in normal lymphoid tissues and its role has been suggested to involve the selection of B and T cells. Here we report three-color flow cytometric studies on the expression of bcl-2 protein in the thymus and periphery. Bcl-2 was expressed in the majority of T and B cells and down-regula...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1006/cimm.1994.1127

    authors: Fujii Y,Okumura M,Takeuchi Y,Inada K,Nakahara K,Matsuda H,Tsujimoto Y

    更新日期:1994-05-01 00:00:00

  • Lipid raft-dependent endocytosis negatively regulates responsiveness of J774 macrophage-like cells to LPS by down regulating the cell surface expression of LPS receptors.

    abstract::Acting through CD14 and TLR4/MD-2, lipopolysaccharide (LPS) triggers strong pro-inflammatory activation of macrophages, which, if not appropriately controlled, may lead to lethal septic shock. Therefore, numerous mechanisms of negative regulation of responses to LPS exist, but whether they include down-regulation of L...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/j.cellimm.2016.11.008

    authors: Józefowski S,Śróttek M

    更新日期:2017-02-01 00:00:00

  • Dexamethasone modulates TCR zeta chain expression and antigen receptor-mediated early signaling events in human T lymphocytes.

    abstract::Dexamethasone is a potent anti-inflammatory and immunosupressive agent that has complex, yet incompletely defined, effects on the immune response. Here, we explored the effect of dexamethasone on the expression of TCR zeta chain and TCR/CD3-induced early signaling events in human T lymphocytes. Immunoblotting studies ...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1006/cimm.2001.1761

    authors: Nambiar MP,Enyedy EJ,Fisher CU,Warke VG,Juang YT,Tsokos GC

    更新日期:2001-02-25 00:00:00

  • Effector and precursor phenotypes of lymphokine-activated killer cells in mice with severe combined immunodeficiency (scid) and athymic (nude) mice.

    abstract::The lineage of lymphokine-activated killer (LAK) cells is poorly understood. To examine the relationship between LAK and natural killer (NK) cells we utilized two congenitally immunodeficient mice, namely severe combined immunodeficient (scid) and athymic (nude) mice that lack T cells but have normal NK cells. LAK act...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(89)90190-1

    authors: Hasui M,Saikawa Y,Miura M,Takano N,Ueno Y,Yachie A,Miyawaki T,Taniguchi N

    更新日期:1989-04-15 00:00:00

  • Inhibition of T cell activation and adhesion functions by soluble CD2 protein.

    abstract::The CD2 (T11) molecule belongs to a family of cell-surface glycoproteins that function as adhesion molecules in the immune system. Human CD2 is found exclusively on cells of the T lineage: peripheral T lymphocytes, NK cells, and thymocytes. CD2 binds specifically to the surface glycoprotein LFA-3. CD2/LFA-3 adhesion i...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1006/cimm.1993.1133

    authors: Rabin EM,Gordon K,Knoppers MH,Luther MA,Neidhardt EA,Flynn JF,Sardonini CA,Sampo TM,Concino MF,Recny MA

    更新日期:1993-06-01 00:00:00

  • Comparative analysis of the heterogeneity of mononuclear cells present in adult and cord blood by simultaneous examination of multiple phenotypic characteristics.

    abstract::In an attempt to better relate specific membrane characteristics of human adult and cord blood lymphocytes to specific functional activities, the phenotypic differences that exist in these two populations have been examined. Cord blood cells have considerably more spontaneous suppressor cell activity than adult cells....

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(83)90214-9

    authors: Johnson C,Dwyer JM

    更新日期:1983-10-01 00:00:00

  • Dialyzable leukocyte extract (transfer factor) in the treatment of superinfected fistulating tuberculosis of the bone.

    abstract::The effect of the addition of dialyzable leukocyte extract (DLE)(transfer factor) to tuberculostatic drugs in the treatment of superinfected fistulating tuberculosis of bones and joints was evaluated in a controlled study. Eleven patients whose disease had persisted for a mean of 20 +/- 4.8 years and had proved to be ...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(84)90091-1

    authors: Zielinski CC,Savoini E,Ciotti M,Orani R,Königswieser H,Eibl MM

    更新日期:1984-03-01 00:00:00

  • Inhibition of proinflammatory macrophage responses and lymphocyte proliferation in vitro by ethyl acetate leaf extract from Daphne gnidium.

    abstract::Medicinal plants are considered immunomodulatory as they display various biological activities. There is no report addressing the anti-inflammatory effects of Daphne gnidium. In this study, we investigated the effects of D. gnidium ethyl acetate (EA) leaf extract on mice immune cell function in vitro. Production of pr...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/j.cellimm.2010.12.002

    authors: Harizi H,Chaabane F,Ghedira K,Chekir-Ghedira L

    更新日期:2011-01-01 00:00:00

  • A proliferation-inducing ligand (APRIL) induced hyper-production of IgA from tonsillar mononuclear cells in patients with IgA nephropathy.

    abstract::IgA nephropathy (IgAN) is a tonsil-related disease. We previously showed that oligodeoxynucleotides with CpG (CpG-ODN) and B-cell activation factor (BAFF) are involved in hyperproduction of IgA from tonsillar mononuclear cells of patients with IgAN (IgAN-TMCs). In this study, we focused on a proliferation-inducing lig...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/j.cellimm.2019.103925

    authors: Takahara M,Nagato T,Nozaki Y,Kumai T,Katada A,Hayashi T,Harabuchi Y

    更新日期:2019-07-01 00:00:00

  • L3T4+ T-cell-independent reactivity of Lyt2+ T cells in vivo.

    abstract::The aim of this study was to analyze in vivo the L3T4+ T-cell-subset-independent reactivity of Lyt2+ T cells toward transplantation alloantigens. To this end, we depleted normal mice of L3T4+ T cells by injection of monoclonal antibodies to the L3T4 antigen. This procedure not only led phenotypically to a disappearanc...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(88)90059-7

    authors: Heeg K,Steeg C,Wagner H

    更新日期:1988-01-01 00:00:00

  • Effects of modulators of adenylyl cyclase on interleukin-2 production, cytosolic Ca2+ elevation, and K+ channel activity in Jurkat T cells.

    abstract::We have studied the effects of prostaglandin E2 (PGE2) and cholera toxin, two modulators of adenylyl cyclase, and 8-bromo cAMP (8-BrcAMP) on various parameters of lymphocyte activation using the human T cell line Jurkat. Our results show that PGE2 and cholera toxin inhibit, in a dose-related manner, the phytohemagglut...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(90)90035-p

    authors: Bastin B,Payet MD,Dupuis G

    更新日期:1990-07-01 00:00:00

  • Ability of T cell subsets and their soluble mediators to modulate the replication of Mycobacterium bovis in bovine macrophages.

    abstract::Peripheral blood mononuclear cells (PBMCs) from cattle vaccinated with Bacillus Calmette-Guerin (BCG) were obtained and expanded in vitro by incubation with purified protein derivative. The ability of these cells to modulate the replication of virulent Mycobacterium bovis in autologous-infected macrophages was compare...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/j.cellimm.2005.01.003

    authors: Denis M,Wedlock DN,Buddle BM

    更新日期:2004-11-01 00:00:00

  • Suppressive effects of soluble histocompatibility antigens on the in vitro generation of cytotoxic T cells to D-end alloantigens.

    abstract::Murine histocompatibility antigens were solubilized from the spleens and lungs of C57BL/6 (H-2b) animals with hypertonic salt (3 M KC1). Aggregate-free soluble antigens were incubated with nonadherent lymph node cells from BALB/c (H-2d) mice for 18 hr prior to their use as responder cells in the mixed-lymphocyte react...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(83)90281-2

    authors: Long PM,Sommer CV,Kong YM

    更新日期:1983-06-01 00:00:00

  • Activation of human T cells with the physiological regulator of protein kinase C.

    abstract::We investigated whether sn-1,2-dioctanoylglycerol (diC8) activates highly purified human T cells. diC8's signaling activity was also compared with that of 12-O-tetradecanoylphorbol-13-acetate (TPA). diC8 and ionomycin were synergistic in promoting T-cell proliferation. The proliferative response was dependent upon an ...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(88)90243-2

    authors: Subramaniam A,Sehajpal P,Murthi VK,Stenzel KH,Suthanthiran M

    更新日期:1988-10-15 00:00:00

  • Toll-like receptor 3 is involved in airway epithelial cell response to nontypeable Haemophilus influenzae.

    abstract::Nontypeable Haemophilus influenzae (NTHi) is the etiological agent most frequently associated with bacterial exacerbations of chronic obstructive pulmonary disease (COPD). The present work shows that NTHi strains induced in primary normal human bronchial epithelial cells (NHBE) a cytokine/chemokine response in which C...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/j.cellimm.2009.09.008

    authors: Teng F,Slavik V,Duffy KE,San Mateo L,Goldschmidt R

    更新日期:2010-01-01 00:00:00

  • Enhanced proliferation of murine T cell lines following interaction of poly(Glu60,Phe40) (GPhe) and antigen-presenting accessory cells. I. GPhe-stimulated enhancement of antigen-dependent proliferative responses.

    abstract::Following interaction of the random polymer (Glu60,Phe40)n (GPhe) with antigen-presenting accessory cells (APC), unusual costimulatory activities were noted in several murine T cell systems. When GPhe, in contrast with other random copolymers (GT,GL), was added during "inhibition" and T cell "repertoire" studies as a ...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(90)90198-z

    authors: Liu DC,Grun JL,Maurer PH

    更新日期:1990-08-01 00:00:00

  • Enhanced engraftment of human peripheral blood lymphocytes into anti-murine interferon-gamma monoclonal antibody-treated C.B.-17-scid mice.

    abstract::To improve the engraftment of human peripheral blood lymphocytes (PBL) to severe combined immunodeficient (SCID) mice, and to elucidate the factors which prevent the PBL's survival, we treated SCID mice with mAb, which neutralizes murine IFN-gamma's ability to activate cell-mediated immunity. Compared with untreated m...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1006/cimm.1997.1238

    authors: Shibata S,Asano T,Noguchi A,Kimura H,Ogura A,Naiki M,Doi K

    更新日期:1998-01-10 00:00:00