Functional rescue of the inactive splice variant of the dopamine D3 receptor D3nf.


:We previously found that the dopamine D3 receptor can be split at the third cytoplasmic loop into two fragments (D3trunk and D3tail), and that the mixture of the two fragments retains the binding and functional activity of the wild type receptor. The dopamine D3 receptor gene gives rise to several inactive receptor splice variants, one of which is the D3nf. Since this gene variant very closely resembles our D3trunk fragment, in this study we investigated if the transfection of D3nf with D3tail could result in the rescue of a functional dopamine receptor. Our experiments showed that D3tail can indeed rescue the activity of D3nf, and that the pharmacological profile of this split D3nf/D3tail receptor is identical to that of the wild type D3 receptor.


Brain Res


Brain research


Scarselli M,Novi F,Corsini GU,Maggio R




Has Abstract


2003-10-17 00:00:00














  • S-allyl cysteine attenuates oxidative stress associated cognitive impairment and neurodegeneration in mouse model of streptozotocin-induced experimental dementia of Alzheimer's type.

    abstract::S-allyl cysteine (SAC), a sulfur containing amino acid derived from garlic, has been reported to have antioxidant, anti-cancer, antihepatotoxic and neurotrophic activity. This study was designed to examine the pre-treatment effects of SAC on cognitive deficits and oxidative damage in the hippocampus of intracerebroven...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Javed H,Khan MM,Khan A,Vaibhav K,Ahmad A,Khuwaja G,Ahmed ME,Raza SS,Ashafaq M,Tabassum R,Siddiqui MS,El-Agnaf OM,Safhi MM,Islam F

    更新日期:2011-05-10 00:00:00

  • Effects of AV3V lesion on pilocarpine-induced pressor response and salivary gland vasodilation.

    abstract::The cholinergic agonist pilocarpine injected intraperitoneally (ip) increases mean arterial pressure (MAP) and superior mesenteric (SM) vascular resistance and reduces submandibular/sublingual gland (SSG) vascular resistance. In the present study, we investigated the effects of electrolytic lesions of the anteroventra...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Takakura AC,Moreira TS,De Luca LA Jr,Renzi A,Menani JV,Colombari E

    更新日期:2005-09-07 00:00:00

  • Recruitment threshold force and its changing type of motor units during voluntary contraction at various speeds in man.

    abstract::The motor unit discharges in human hand muscles were recorded during voluntary isometric contraction. Bipolar wire electrodes were inserted in the right adductor pollicis muscle (AP) and first dorsal interosseous muscle (FDI) for the recordings. Motor unit activities from these muscles were investigated when subjects ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Yoneda T,Oishi K,Fujikura S,Ishida A

    更新日期:1986-04-30 00:00:00

  • Relationship of neuronal discharges in the precentral gyrus of monkeys to the performance of arm movements.

    abstract::Recordings have been made from 162 pyramidal tract neurones which discharged bursts of nerve impulses in characteristic temporal association with performances of a stereotyped motor task by monkeys. Clinical evaluation of the relationship between discharges of the neurones and free movement led to the view that each c...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Porter R,Lewis MM

    更新日期:1975-11-07 00:00:00

  • The morphogenesis of glutamic acid decarboxylase in the neostriatum of the cat: neuronal and ultrastructural localization.

    abstract::Correlative light and electron microscopic immunohistochemical methods were adapted for a descriptive analysis of the normal time course and pattern of expression and intraneuronal localization of the enzyme glutamic acid decarboxylase (GAD) in the neostriatum (Ns) of fetal, postnatal and adult cats. The differentiati...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Fisher RS,Levine MS,Adinolfi AM,Hull CD,Buchwald NA

    更新日期:1987-06-01 00:00:00

  • Electrophysiological properties of spinally-projecting A5 noradrenergic neurons.

    abstract::Spinally-projecting A5 neurons were studied with anatomical and electrophysiological techniques in the rat. A detailed study of the number and distribution of spinally-projecting catecholaminergic (CA) and non-catecholaminergic neurons present in a defined area of ventrolateral pontine reticular formation was performe...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Byrum CE,Stornetta R,Guyenet PG

    更新日期:1984-06-11 00:00:00

  • Reduced cerebral monocarboxylate transporters and lactate levels by ethanol and normobaric oxygen therapy in severe transient and permanent ischemic stroke.

    abstract:OBJECTIVES:Neuroprotective benefits of ethanol (EtOH) and normobaric oxygenation (NBO) were previously demonstrated in transient and permanent ischemic stroke. Here we sought to identify whether the enhanced lactic acidosis and increased expression of monocarboxylate transporters (MCTs) observed after stroke might be a...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Geng X,Sy CA,Kwiecien TD,Ji X,Peng C,Rastogi R,Cai L,Du H,Brogan D,Singh S,Rafols JA,Ding Y

    更新日期:2015-04-07 00:00:00

  • Behavioral model for diffuse noxious inhibitory controls (DNIC): potentiation by 5-hydroxytryptophan.

    abstract::The effects of the serotonin precursor 5-HTP, were determined in a behavioral DNIC paradigm (increase in vocalization threshold after intraperitoneal injection of the algogenic agent, phenylbenzoquinone). This counter-irritation phenomenon was strongly potentiated by 5-HTP, such potentiation being blocked by the 5-HT ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Kraus E,Besson JM,Le Bars D

    更新日期:1982-01-14 00:00:00

  • Microdialysates of amines and metabolites from core nucleus accumbens of freely moving rats are altered by dizocilpine.

    abstract::In vivo microdialysis combined with high performance liquid chromatography (HPLC) with electrochemical detection, was used to study the effect of MK-801 (0.1 mg/kg i.p.) on extracellular concentrations of dopamine (DA) 3,4-dihydroxyphenylacetic acid (DOPAC), serotonin (5-HT), norepinephrine (NE) and DOPAC/DA ratio in ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Hatip-Al-Khatib I,Mishima K,Iwasaki K,Fujiwara M

    更新日期:2001-05-25 00:00:00

  • Alterations in tyrosine hydroxylase activity elicited by raphe nuclei lesions in the rat locus coeruleus: evidence for the involvement of serotonin afferents.

    abstract::The time course of the variations in tyrosine hydroxylase (TH) activity was measured in the rat locus coeruleus (LC) after lesions of the nucleus raphe dorsalis (NRD), nucleus raphe centralis superior (NRCS) and nucleus raphe pontis (NRP). A certain number of lesions were performed in the raphe magnus (RM), the caudal...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: McRae-Degueurce A,Berod A,Mermet A,Keller A,Chouvet G,Joh TH,Pujol JF

    更新日期:1982-03-11 00:00:00

  • Somatostatin inhibition of adenylate cyclase activity in different brain areas.

    abstract::Somatostatin receptors have been identified in different brain areas but the characterization of their postreceptor effect is still lacking. In this study we analyze the somatostatin effect on adenylate cyclase activity in different brain regions, namely frontal cortex, striatum, hypothalamus and hippocampus. Somatost...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Schettini G,Florio T,Meucci O,Landolfi E,Grimaldi M,Ventra C,Marino A

    更新日期:1989-07-17 00:00:00

  • The effects of stimuli on the activity and functional connectivity of local neuronal groups in the cat auditory cortex.

    abstract::Simultaneous extracellular recordings from one electrode of 'local' groups of 3-6 neurons were obtained from the auditory cortex of unanesthetized, paralyzed cats. The activity and functional connectivity of local microenvironments were examined under various auditory stimuli. Single cell response patterns were examin...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Frostig RD,Gottlieb Y,Vaadia E,Abeles M

    更新日期:1983-08-08 00:00:00

  • Effects of repeated injections of the neurotensin analog NT69L on dopamine release and uptake in rat striatum in vitro.

    abstract::The effect of five daily intraperitoneal (i.p.) injections of NT69L on in vitro dopamine release, uptake, and [(3)H]NT binding in rat striatal tissue was investigated. NT69L perfusion increased K(+)-evoked and electrically evoked [(3)H]DA release. NT receptor-1 antagonist SR48692 inhibited the stimulatory effect of NT...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Wang R,Boules M,Tiner W,Richelson E

    更新日期:2004-10-29 00:00:00

  • Quantitative autoradiography of adenosine receptors and NBTI-sensitive adenosine transporters in the brains and spinal cords of mice deficient in the mu-opioid receptor gene.

    abstract::There is a large body of evidence indicating important interactions between the adenosine and opioid systems in regulating pain at both the spinal and supraspinal level. Mice lacking the mu-opioid receptor (MOR) gene have been successfully developed and the animals show complete loss of analgesic responses to morphine...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Bailey A,Matthes H,Kieffer B,Slowe S,Hourani SM,Kitchen I

    更新日期:2002-07-05 00:00:00

  • Glycine-induced changes in synaptic efficacy in hippocampal slices involve changes in AMPA receptors.

    abstract::Brief applications of high glycine concentrations to hippocampal slices have been shown to produce long-lasting changes in synaptic efficacy. In the present study, we show that glycine application transiently and reversibly increases the amplitude and prolongs the duration of synaptic potentials mediated by N-methyl-D...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Shahi K,Baudry M

    更新日期:1993-11-12 00:00:00

  • Development of testosterone-metabolizing pathways in the avian brain: enzyme localization and characteristics.

    abstract::Metabolites formed from testosterone in the brain, their neuroanatomical distribution and the kinetic properties of the enzymes were studied in male and female Japanese quail chicks and compared with the adult brain using an in vitro radioassay. Oestradiol-17 beta. 5 alpha-dihydrotestosterone, 5 beta-dihydrotestostero...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Hutchison JB,Schumacher M

    更新日期:1986-02-01 00:00:00

  • Release of amino acids into regional superfusates of the spinal cord by mechano-stimulation of the reproductive tract.

    abstract::Based on pharmacological evidence that inhibitory amino acids mediate vaginocervical mechano-stimulation produced analgesia (VSPA), we hypothesized that inhibitory amino acids would be released endogenously in the spinal cord in response to vaginocervical mechano-stimulation (VS). This hypothesis was tested by HPLC an...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Masters DB,Jordan F,Beyer C,Komisaruk BR

    更新日期:1993-09-10 00:00:00

  • Amylin-leptin coadministration stimulates central histaminergic signaling in rats.

    abstract::Combined amylin+leptin (AMN+LEP) can reduce diet induced obesity and is very effective in combating LEP resistance. The purpose of this study was to evaluate the effect of AMN+LEP on central histaminergic signaling in lean and obese rats. Male rats were administered LEP (300 μg/kg/d), AMN (100 μg/kg/d), AMN+LEP or veh...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Seth R,Terry DE,Parrish B,Bhatt R,Overton JM

    更新日期:2012-03-09 00:00:00

  • Metabotropic glutamate receptor/phospholipase C system in female rat heart.

    abstract::Glutamate is the main excitatory neurotransmitter in the central nervous system. This amino acid mediates learning and memory processes acting through ionotropic and metabotropic receptor binding. Metabotropic glutamate receptors (mGluRs) are G protein-coupled receptors that stimulate phospholipase C (PLC) or inhibit ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Iglesias I,Castillo CA,León D,Ruíz MA,Albasanz JL,Martín M

    更新日期:2007-06-11 00:00:00

  • (2S,1'R,2'R,3'R)-2-(2,3-dicarboxycyclopropyl) glycine positively modulates metabotropic glutamate receptors coupled to polyphosphoinositide hydrolysis in rat hippocampal slices.

    abstract::In rat hippocampal slices, the novel metabotropic glutamate receptor (mGluR) ligand, (1S,1'R,2'R,3'R)-2-(2,3-dicarboxycyclopropyl)glycine (DCG-IV) enhanced the stimulation of polyphosphoinositide (PPI) hydrolysis elicited by quisqualate or by submaximal concentrations of ibotenate or (1S,3R)-1-aminocyclopentane-1,3-di...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Genazzani AA,L'Episcopo MR,Casabona G,Shinozaki H,Nicoletti F

    更新日期:1994-10-03 00:00:00

  • Time- and frequency-dependent effects of potassium channel blockers on large and medium diameter optic tract axons.

    abstract::Compound action potential recording techniques were used to investigate the time- and frequency-dependent effects of 4-aminopyridine (4-AP) and tetraethylammonium (TEA) on large diameter, fast conducting (t1) and medium diameter, middle conducting (t2) optic tract axons in anesthetized hooded rats. Single-pulse studie...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Fox DA,Ruan DY

    更新日期:1989-10-02 00:00:00

  • Discrimination between changes in glucocorticoid receptor expression and activation in rat brain using western blot analysis.

    abstract::These studies investigated autoregulation of glucocorticoid receptor (GR) protein expression and activation in rat brain using western blot methodology. By comparing GR immunoblot reactivity present in various tissue subcellular preparations we were able to discriminate between corticosterone-induced changes in GR act...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Spencer RL,Kalman BA,Cotter CS,Deak T

    更新日期:2000-06-23 00:00:00

  • Abnormal myelinogenesis both in the white and gray matter of the attractin-deficient mv rat.

    abstract::The myelin vacuolation (mv) rat exhibits hypomyelination and vacuole formation in the myelin throughout the CNS, caused by a null mutation in the attractin gene. Myelin alterations in the spinal cord of mv rats progress during postnatal development and are more prominent in the white matter. In contrast, microglial ac...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Izawa T,Yamate J,Franklin RJ,Kuwamura M

    更新日期:2010-02-02 00:00:00

  • Generalization of seizures parallels the formation of "dark" neurons in the hippocampus and pontine reticular formation after focal-cortical application of 4-aminopyridine (4-AP) in the rat.

    abstract::Distribution and time course of the occurrence of "dark" neurons were compared with the EEG activity and behavior of rats during 4-aminopyridine (4-AP) induced epileptic seizures. A crystal of the K(+) channel blocker 4-AP (0.5 mg/kg) was placed onto the exposed parieto-occipital cortex of Halothane-anesthetized rats ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Baracskay P,Szepesi Z,Orbán G,Juhász G,Czurkó A

    更新日期:2008-09-04 00:00:00

  • Monosodium glutamate: increased neurotoxicity after removal of neuronal re-uptake sites.

    abstract::Microinjections of monosodium glutamate (MSG; 300 microgram/0.5 microliter) into the hippocampus of the adult rat result in only marginal damage to local neurons. Perforant path transections, removing glutamatergic afferents to hippocampal granule cells, make the latter markedly more vulnerable to a subsequent MSG inj...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Köhler C,Schwarcz R

    更新日期:1981-05-04 00:00:00

  • The effects of aging on muscarinic receptor/G-protein coupling in the rat hippocampus and striatum.

    abstract::In the striatum and hippocampus, there is a loss of sensitivity to muscarinic agonists with age which has been traced to events early in the signal transduction pathway. Our laboratory has therefore focussed on investigations at this level. The current experiments investigate the effects of age on G-protein/receptor i...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Anson RM,Cutler R,Joseph JA,Yamagami K,Roth GS

    更新日期:1992-12-11 00:00:00

  • Heterogeneity of neurons in the crustacean X-organ as revealed by intracellular recording and injection of horseradish peroxidase.

    abstract::The intracellular injection technique of HRP with simultaneous recordings of intracellular potential was applied to the crab X-organ-sinus gland peptidergic neurosecretory neurons. At least two classes of neurons were discriminated from the usual type of neurosecretory neurons morphologically as well as electrophysiol...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Nagano M

    更新日期:1986-01-08 00:00:00

  • Dose-independent antinociceptive interaction of endogenous ligands at the spinal level.

    abstract::Adenosine, agmatine and kynurenic acid are endogenous ligands acting on different (e.g. adenosine, NMDA, alpha(2)-adrenergic and imidazoline) receptors with a potential role in nociception at the spinal level. Their antinociceptive effects have already been investigated as monotherapy, but only a few studies have repo...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Kekesi G,Joo G,Csullog E,Peter-Szabo M,Benedek G,Horvath G

    更新日期:2004-12-10 00:00:00

  • Oligodendroglial pathology in the development of myelin breakdown in the dmy mutant rat.

    abstract::The dmy rat is an autosomal recessive mutant that exhibits severe myelin destruction throughout the white matter of the central nervous system. Recently, a point mutation in intron 3 of the Mrs2 has been found in the dmy rat. Mrs2 encodes an essential component of the major electrophoretic Mg(2+) influx system in mito...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Kuwamura M,Inumaki K,Tanaka M,Shirai M,Izawa T,Yamate J,Franklin RJ,Kuramoto T,Serikawa T

    更新日期:2011-05-10 00:00:00

  • Differential modulation of the glutamate transporters GLT1, GLAST and EAAC1 by docosahexaenoic acid.

    abstract::At present, the ability of polyunsaturated fatty acids (PUFAs) to regulate individual glutamate transporter subtypes is poorly understood and very little information exists on the mechanism(s) by which PUFAs achieve their effects on the transport process. Here we investigate the effect of cis-4,7,10,13,16,19-docosahex...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Berry CB,Hayes D,Murphy A,Wiessner M,Rauen T,McBean GJ

    更新日期:2005-03-10 00:00:00