Abstract:
:Expression of the human beta-myosin heavy chain (beta MHC) gene was studied by transient assay in culture and in situ by direct injection of plasmids into adult rat hearts. In this report we describe a unique repressor region located -326/-309 (5'-TTGGTGGTCGTGGTCAGT-3') of the human beta MHC gene that is conserved among the rat, rabbit, and human beta MHC genes. This sequence conferred repression onto heterologous promoters when the sequence was located 5' but not 3' to the promoters. This partial positional dependency suggests that the factor may act by limiting the binding of enhancers, located more proximally, to their DNA binding sites.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Edwards JG,Bahl JJ,Flink I,Milavetz J,Goldman S,Morkin Edoi
10.1016/0006-291x(92)91586-fsubject
Has Abstractpub_date
1992-11-30 00:00:00pages
504-10issue
1eissn
0006-291Xissn
1090-2104pii
0006-291X(92)91586-Fjournal_volume
189pub_type
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